Computer vision uncovers predictors of physical urban change

Which neighborhoods experience physical improvements? In this paper, we introduce a computer vision method to measure changes in the physical appearances of neighborhoods from time-series street-level imagery. We connect changes in the physical appearance of five US cities with economic and demographic data and find three factors that predict neighborhood improvement. First, neighborhoods that are densely populated by college-educated adults are more likely to experience physical improvements—an observation that is compatible with the economic literature linking human capital and local success. Second, neighborhoods with better initial appearances experience, on average, larger positive improvements—an observation that is consistent with “tipping” theories of urban change. Third, neighborhood improvement correlates positively with physical proximity to the central business district and to other physically attractive neighborhoods—an observation that is consistent with the “invasion” theories of urban sociology. Together, our results provide support for three classical theories of urban change and illustrate the value of using computer vision methods and street-level imagery to understand the physical dynamics of cities

Outcomes after angiography with sodium bicarbonate and acetylcysteine

Background: Intravenous sodium bicarbonate and oral acetylcysteine are widely used to prevent acute kidney injury and associated adverse outcomes after angiography without definitive evidence of their efficacy. Methods: Using a 2-by-2 factorial design, we randomly assigned 5177 patients at high risk for renal complications who were scheduled for angiography to receive intravenous 1.26% sodium bicarbonate or intravenous 0.9% sodium chloride and 5 days of oral acetylcysteine or oral placebo; of these patients, 4993 were included in the modified intention-to-treat analysis. The primary end point was a composite of death, the need for dialysis, or a persistent increase of at least 50% from baseline in the serum creatinine level at 90 days. Contrast-associated acute kidney injury was a secondary end point. Results: The sponsor stopped the trial after a prespecified interim analysis. There was no interaction between sodium bicarbonate and acetylcysteine with respect to the primary end point (P=0.33). The primary end point occurred in 110 of 2511 patients (4.4%) in the sodium bicarbonate group as compared with 116 of 2482 (4.7%) in the sodium chloride group (odds ratio, 0.93; 95% confidence interval [CI], 0.72 to 1.22; P=0.62) and in 114 of 2495 patients (4.6%) in the acetylcysteine group as compared with 112 of 2498 (4.5%) in the placebo group (odds ratio, 1.02; 95% CI, 0.78 to 1.33; P=0.88). There were no significant between-group differences in the rates of contrast-associated acute kidney injury. Conclusions: Among patients at high risk for renal complications who were undergoing angiography, there was no benefit of intravenous sodium bicarbonate over intravenous sodium chloride or of oral acetylcysteine over placebo for the prevention of death, need for dialysis, or persistent decline in kidney function at 90 days or for the prevention of contrast-associated acute kidney injury. (Funded by the U.S. Department of Veterans Affairs Office of Research and Development and the National Health and Medical Research Council of Australia; PRESERVE ClinicalTrials.gov number, NCT01467466.

Multiplexed enrichment and genomic profiling of peripheral blood cells reveal subset-specific immune signatures

Specialized immune cell subsets are involved in autoimmune disease, cancer immunity, and infectious disease through a diverse range of functions mediated by overlapping pathways and signals. However, subset-specific responses may not be detectable in analyses of whole blood samples, and no efficient approach for profiling cell subsets at high throughput from small samples is available. We present a low-input microfluidic system for sorting immune cells into subsets and profiling their gene expression. We validate the system’s technical performance against standard subset isolation and library construction protocols and demonstrate the importance of subset-specific profiling through in vitro stimulation experiments. We show the ability of this integrated platform to identify subset-specific disease signatures by profiling four immune cell subsets in blood from patients with systemic lupus erythematosus (SLE) and matched control subjects. The platform has the potential to make multiplexed subset-specific analysis routine in many research laboratories and clinical settings.National Institute of Allergy and Infectious Diseases (U.S.) (Grant U24 AI118668

Hyperbaric oxygen in the management of wound tissue necrosis after external dacrocystorhinostomy

Hyperbaric oxygen is an adjunctive treatment for promoting wound healing and reducing infection. We present an unusual case of wound tissue necrosis occurring after external dacryocystorhinostomy (ExtDCR) that was subsequently treated with hyperbaric oxygen (HBO) and advancement flaps with good outcome. HBO improves vascularization of ischemic tissues after ExtDCR for greater success after reconstructive surgery

Self-starting cumulative sum harvest control rule (SS-CUSUM-HCR) for status-quo management of data-limited fisheries

We demonstrate a harvest control rule based on the self-starting cumulative sum (SS-CUSUM) control chart that can maintain a fish stock at its starting (status-quo) level. The SS-CUSUM is an indicator monitoring tool commonly used in quality control engineering and does not require a long time series or predefined reference point for detecting temporal trends. The reference points in SS-CUSUM are calibrated in the form of running means that are updated on an ongoing basis when new observations become available. The SS-CUSUM can be initiated with as few as two observations in the time series and can be applied long before many other methods, soon after initial data become available. A wide range of stock indicators can be monitored, but in this study, we demonstrate the method using an equally weighted sum of two indicators: a recruitment indicator and a large fish indicator from a simulated fishery. We assume that no life history data are available other than 2 years of both indicator data and current harvest levels when the SS-CUSUM initiates. The signals generated from SS-CUSUM trigger a harvest control rule (SS-CUSUM-HCR), where the shift that occurs in the indicator time series is computed and is used as an adjustment factor for updating the total allowable catch. Our study shows that the SS-CUSUM-HCR can maintain the fish stock at its starting status-quo level (even for overfished initial states) but has limited scope if the fishery is already in an undesirable state such as a stock collapse. We discuss how the SS-CUSUM approach could be adapted to move beyond a status-quo management strategy, if additional information on the desirable state of the fishery is available. </jats:p

Some theoretical and practical possibilities of plant genetic manipulation using protoplasts

Protoplasts capable of division and plant regeneration are now available for a large number of vegetable, oil and forage crops. However, routine hybrid production is not possible due to methodological limitations in selection and culture of hybrid cells. Recent improvement in techniques are the use of a double mutant as a universal hybridizer and the use of fluorescence activated cell sorter to recover hybrid cells. Interest is also centered on limited gene transfer by protoplast fusion. We propose a model of generating triploid plants by somatic cell fusion to transfer limited genomic information from an alien plant to a crop plant. Somatic hybridization has some novel features but, in practice and conception, it is an extension of the methods of sexual hybridization. By contrast, genetic transformation is a radically different approach to plant genetic manipulation. The success of this approach will depend upon how readily genotype can be related to phenotype in a tangible way so as to ascertain what biochemical and developmental activity is controlled or modulated by a DNA sequence

The immune cell landscape in kidneys of patients with lupus nephritis.

Lupus nephritis is a potentially fatal autoimmune disease for which the current treatment is ineffective and often toxic. To develop mechanistic hypotheses of disease, we analyzed kidney samples from patients with lupus nephritis and from healthy control subjects using single-cell RNA sequencing. Our analysis revealed 21 subsets of leukocytes active in disease, including multiple populations of myeloid cells, T cells, natural killer cells and B cells that demonstrated both pro-inflammatory responses and inflammation-resolving responses. We found evidence of local activation of B cells correlated with an age-associated B-cell signature and evidence of progressive stages of monocyte differentiation within the kidney. A clear interferon response was observed in most cells. Two chemokine receptors, CXCR4 and CX3CR1, were broadly expressed, implying a potentially central role in cell trafficking. Gene expression of immune cells in urine and kidney was highly correlated, which would suggest that urine might serve as a surrogate for kidney biopsies

EN-BIRTH Data Collector Training - Handbook and Manual

The EN-BIRTH study aims to validate selected newborn and maternal indicators for routine facility-based tracking of coverage and quality of care for use at district, national and global levels. The item contains the EN-BIRTH_Trainer's Manual (14 June 2017) and EN-BIRTH_Training Handbook (23 May 2017)