Illegal Intrusion Detection for In-Vehicle CAN Bus Based on Immunology Principle

The controller area network (CAN) bus has become one of the most commonly used protocols in automotive networks. Some potential attackers inject malicious data packets into the CAN bus through external interfaces for implementing illegal operations (intrusion). Anomaly detection is a technique for network intrusion detection which can detect malicious data packs by comparing the normal data packets with incoming data packets obtained from the network traffic. The data of a normal network is in a symmetric and stable state, which will become asymmetric when compromised. Considering the in-vehicle network, the CAN bus is symmetrically similar to the immune system in terms of internal network structure and external invasion threats. In this work, we use an intrusion detection method based on the dendritic cell algorithm (DCA). However, existing studies suggest the use of optimization methods to improve the accuracy of classification algorithms, and the current optimization of the parameters of the detection method mostly relies on the manual tuning of the parameters, which is a large workload. In view of the above challenges, this paper proposes a new detection algorithm based on the particle swarm optimization algorithm (PSO) and gravitational search algorithm (GSA) to improve the dendritic cell algorithm (PSO-GSA-DCA). PSO-GSA-DCA achieves adaptive parameter tuning and improves detection accuracy by mixing optimization algorithms and using them to optimize the dendritic cell algorithm classifier. Additionally, DCA-based CAN message attribute matching rules (measured by information gain and standard deviation of CAN data) are proposed for matching the three input signals (PAMP, DS, SS) of the DCA. The experimental results show that our proposed scheme has a significant improvement in accuracy, which can reach 91.64%, and lower time loss compared with other correlation anomaly detection schemes. Our proposed method also enables adaptive tuning, which solves the problem that most models now rely on manual tuning

Soil, Plant, and Microorganism Interactions Drive Secondary Succession in Alpine Grassland Restoration

Plant secondary succession has been explored extensively in restoring degraded grasslands in semiarid or dry environments. However, the dynamics of soil microbial communities and their interactions with plant succession following restoration efforts remain understudied, particularly in alpine ecosystems. This study investigates the interplay between soil properties, plant communities, and microbial populations across a chronosequence of grassland restoration on the Qinghai–Tibet Plateau in China. We examined five succession stages representing artificial grasslands of varying recovery durations from 0 to 19. We characterized soil microbial compositions using high-throughput sequencing, enzymatic activity assessments, and biomass analyses. Our findings reveal distinct plant and microbial secondary succession patterns, marked by increased soil organic carbon, total phosphorus, and NH4+-N contents. Soil microbial biomass, enzymatic activities, and microbial community diversity increased as recovery time progressed, attributed to increased plant aboveground biomass, cover, and diversity. The observed patterns in biomass and diversity dynamics of plant, bacterial, and fungal communities suggest parallel plant and fungal succession occurrences. Indicators of bacterial and fungal communities, including biomass, enzymatic activities, and community composition, exhibited sensitivity to variations in plant biomass and diversity. Fungal succession, in particular, exhibited susceptibility to changes in the soil C: N ratio. Our results underscore the significant roles of plant biomass, cover, and diversity in shaping microbial community composition attributed to vegetation-induced alterations in soil nutrients and soil microclimates. This study contributes valuable insights into the intricate relationships driving secondary succession in alpine grassland restoration

Establishment of a novel alloxan‐induced rabbit model exhibiting unique diabetic retinal neuropathy features assessed via ERG + VEP

Abstract Background Diabetic retinal neuropathy (DRN) leads to significant visual impairment; however, no existing animal model fully replicates its neural alterations, and inconsistent induction protocols with high mortality rates hinder long‐term investigations. Methods Adult male rabbits were randomly assigned to four experimental groups, each receiving a single intravenous injection of varying doses of alloxan and one control group. The safety and efficacy of alloxan in inducing diabetes were evaluated to determine the optimal dose. At 9 weeks following injection with alloxan, retinal function was assessed using full‐field electroretinography (ERG) and visual evoked potentials (VEPs). Retinal structure was examined in rabbits using spectral‐domain optical coherence tomography (SD‐OCT), Optos ultra‐widefield (Optos UWF) false‐color imaging, and widefield fundus fluorescein angiography (WF‐FFA). Results Rabbits in the 80 mg/kg alloxan group exhibited fewer complications, lower mortality, and a higher model success rate compared to other groups. At 9 weeks post‐injection, these rabbits demonstrated significantly elevated hemoglobin A1c and total cholesterol (p < 0.05) relative to controls. ERG revealed statistically significant reductions in oscillatory potential and b‐wave amplitudes (p < 0.05), while VEP indicated decreased P2 amplitude (p < 0.001) and prolonged P2 latency (p < 0.05). SD‐OCT, Optos UWF imaging, and WF‐FFA demonstrated no significant changes in vascular abnormalities. Additionally, Hematoxylin and Eosin staining revealed retinal swelling (p < 0.05), and immunofluorescence confirmed glial activation and neuronal loss. Conclusions A single intravenous injection of 80 mg/kg alloxan effectively and safely induced DRN in rabbits, resulting in neural retina damage, thereby establishing this model as an ideal model for DRN research

Fossil fruits of <i>Trapa</i> L. from the late Miocene of southeastern Qaidam Basin (Qinghai, China)

Trapa L. (Lythraceae J. ST.-HIL) is a genus of annual free-floating plant, widely distributed in the Northern Hemisphere. The Qaidam Basin is regarded as an ideal region to understand the Cenozoic palaeoclimatic and palaeobiological evolution of the northern Tibetan Plateau. A few records of Trapa have been previously mentioned from the Cenozoic deposits of the Qaidam Basin, northern Tibetan Plateau. Here, we describe some fossil fruits of Trapa from the late Miocene Shangyoushashan Formation of southeastern Qaidam Basin (Tuosu Lake area), which were identified as Trapa qaidamensis sp. nov. based on their morphology. Extant species of Trapa usually live in the environments with mean temperature of warmest quarter (MTWQ) ranging from 14.9°C to 30.9°C, as well as mean temperature of warmest month (MTWM) ranging from 19.6°C to 37.3°C in the living areas of Trapa. Compared with MTWQ 16.5°C and MTWM 17.5°C in current fossil locality, the present fossil site was warmer during the Miocene than in the present day. urn:lsid:zoobank.org:act:9F7DAD1B-DA2D-4734-BE27-99276F57DD2A</p

A new cyprinid from the Oligocene of Qaidam Basin, north-eastern Tibetan plateau, and its implications

The Qaidam Basin, lying in the north-eastern Tibetan Plateau, China, is key to understanding the Cenozoic climatic and biological changes that have occurred on the plateau; however, information from a palaeontological perspective on this Palaeogene basin is scant. Recently, fossil cyprinids were found in the middle portion of the Shangganchaigou Formation (= Upper Ganchaigou Formation; Oligocene) in the north-western Qaidam Basin. These share many osteological characters with barbines and ‘morphologically primitive clade schizothoracines’ (= primitive schizothoracines; i.e. Schizothorax and Aspiorhynchus), and they closely resemble the latter in the size and shape of the scales: body scales are small and oval; the number of lateral line scales exceeds 100; and the long-oval scales from the pre-anal region are very similar to the ‘anal scales’, which are unique to schizothoracines. Compared with another fossil fish, Paleoschizothorax qaidamensis, from the same formation, the major differences are: (1) the posterior part of the entopterygoid in the new material is normal and not expanded while that of P. qaidamensis is obviously expanded; and (2) the premaxilla process of maxilla is well developed in P. qaidamensis but weak in the new specimens. Therefore, they are described as a new species of the fossil genus Paleoschizothorax (subfamily Schizothoracinae): Paleoschizothorax diluculum sp. nov. A phylogenetic analysis, which included 13 extant genera and three fossil forms of barbines and schizothoracines and 70 morphological characters, also supports the close relationship between P. diluculum and primitive schizothoracines. A preliminary correlation analysis suggests that the degree of reduction of body scale size is negatively correlated with habitat mean temperatures among most Chinese cyprinids. Moreover, we speculate that the ‘primitive schizothoracines’ likely originated in the north-eastern–central Tibetan Plateau based on the fossil records and molecular phylogeny of the extant taxa. http://zoobank.org/urn:lsid:zoobank.org:pub:C461C7C0-9BAB-44DD-8DEE-0B5BB03D6479</p

A new species of Pimplinae (Hymenoptera: Ichneumonidae) from the Oligocene of Huatugou area, Qaidam Basin, Tibetan Plateau, China

A new species Theronia qaidamensis sp. nov. (Ichneumonidae, Pimplinae) is described from the Oligocene Shangganchaigou Formation of the northwestern Qaidam Basin (Huatugou area), northeast of the Tibetan Plateau (TP). The new species represents the first report of the Ichneumonidae from the Qaidam Basin, which reveals an insect linkage between the Qaidam Basin and Europe. In addition, the study of associated fossils from the Shangganchaigou Formation shows that the palaeoenvironment of the northwestern Qaidam Basin during the Oligocene was warm and moist, which provided suitable living conditions for the current fossil.</p