Butterfly learning and the diversification of plant leaf shape

Visual cues are important for insects to find flowers and host plants. It has been proposed that the diversity of leaf shape in Passiflora vines could be a result of negative frequency dependent selection driven by visual searching behavior among their butterfly herbivores. Here we tested the hypothesis that Heliconius butterflies use leaf shape as a cue to initiate approach toward a host plant. We first tested for the ability to recognize shapes using a food reward conditioning experiment. Butterflies showed an innate preference for flowers with three and five petals. However, they could be trained to increase the frequency of visits to a non-preferred flower with two petals, indicating an ability to learn to associate shape with a reward. Next we investigated shape learning specifically in the context of oviposition by conditioning females to lay eggs on two shoots associated with different artificial leaf shapes: their own host plant, Passiflora biflora, and a lanceolate non-biflora leaf shape. The conditioning treatment had a significant effect on the approach of butterflies to the two leaf shapes, consistent with a role for shape learning in oviposition behavior. This study is the first to show that Heliconius butterflies use shape as a cue for feeding and oviposition, and can learn shape preference for both flowers and leaves. This demonstrates the potential for Heliconius to drive negative frequency dependent selection on the leaf shape of their Passiflora host plants

The diversification of Heliconius butterflies: what have we learned in 150 years?

Research into Heliconius butterflies has made a significant contribution to evolutionary biology. Here, we review our understanding of the diversification of these butterflies, covering recent advances and a vast foundation of earlier work. Whereas no single group of organisms can be sufficient for understanding life's diversity, after years of intensive study, research into Heliconius has addressed a wide variety of evolutionary questions. We first discuss evidence for widespread gene flow between Heliconius species and what this reveals about the nature of species. We then address the evolution and diversity of warning patterns, both as the target of selection and with respect to their underlying genetic basis. The identification of major genes involved in mimetic shifts, and homology at these loci between distantly related taxa, has revealed a surprising predictability in the genetic basis of evolution. In the final sections, we consider the evolution of warning patterns, and Heliconius diversity more generally, within a broader context of ecological and sexual selection. We consider how different traits and modes of selection can interact and influence the evolution of reproductive isolation.RMM is funded by a Junior Research Fellowship at King’s College, Cambridge. KMK is supported by the Balfour Studentship, University of Cambridge, SHMa by a Research Fellowship at St John's College, Cambridge, and SHMo by a Research Fellowship from the Royal Commission for the Exhibition of 1851. Our work on Heliconius has been additionally supported by the Agence Nationale de la Recherche (France), the Biology and Biotechnology Research Council (UK), the British Ecological Society, the European Research Council, the Natural Environment Research Council (UK), and the Smithsonian Tropical Research Institute.This is the author accepted manuscript. The final version is available from Wiley via http://dx.doi.org/10.1111/jeb.1267

Psychological Indicators and Perceptions of Adolescents in Residential Care

Abstract The institutionalization of adolescents has been mentioned in the literature with positive and negative aspects. This study investigated 61 adolescents in residential care aiming to evaluate psychological problems and perceptions related to the care, using interviews and the YSR. Data was evaluated using quantitative and qualitative analysis, using gender, age and length of institutionalization as variables. Results indicated clinical scores for psychological problems, except for externalizing problems, in younger girls recently taken into care and older boys institutionalized for longer periods. The perceptions about the care were negative or indifferent; elements of positivity were expressed by adolescents institutionalized for longer periods. Instability in the permanence in the care service and the reasons for having been taken into care were manifested with psychological distress. There were few contacts with the family of origin; the adolescents mentioned the importance of their families and the desire to leave the institution. Members of the institutional teams were indicated as sources of care and protection. This study reflects the challenges for the positive development of this population

Avoidance of an aposematically coloured butterfly by wild birds in a tropical forest.

1. Birds are considered to be the primary selective agents for warning colouration in butterflies, and select for aposematic mimicry by learning to avoid brightly coloured prey after unpleasant experiences. It has long been thought that bright colouration plays an important role in promoting the avoidance of distasteful prey by birds. 2. The hypothesis that warning colouration facilitates memorability and promotes predator avoidance was tested by means of a field experiment using distasteful model butterflies. Artificial butterflies with a Heliconius colour pattern unknown to local birds were generated using bird vision models, either coloured or achromatic, and hung in tree branches in a tropical forest. Two sequential trials were conducted at each site to test avoidance by naïve and experienced predators. 3. There was a significant reduction in predation in the second trial. Also, coloured models were attacked less than achromatic models. Specifically, coloured butterflies were attacked significantly less in the second trial, but there was no significant decrease in predation on achromatic models. 4. The present results imply an important role for colour in enhancing aversion of aposematic butterflies. It has also been demonstrated that previous experience of distasteful prey can lead to enhanced avoidance in subsequent trials, supporting mimicry theory.CAPES Cambridge Trust Biotechnology and Biological Sciences Research Council (BBSRC) David Phillips Research Fellowship. Grant Number: BB/G022887/

Sintomas depressivos e eventos estressores em crianças e adolescentes no contexto de institucionalização Depressive symptoms and stressful events in children and adolescents in the institutionalized context

INTRODUÇÃO: O presente estudo verificou a manifestação de sintomas depressivos e a freqüência e o impacto de eventos estressores em crianças e adolescentes institucionalizados e não-institucionalizados. MÉTODO: Participaram 257 jovens de 7 a 16 anos de ambos os sexos, sendo que 130 residiam em abrigos de proteção e 127 moravam com suas famílias na região metropolitana de Porto Alegre. Todos os participantes freqüentavam da 1ª à 8ª série do ensino fundamental de escolas públicas localizadas em bairros de baixas condições socioeconômicas. Foram aplicados os instrumentos Children's Depression Inventory e o Inventário de Eventos Estressores na Infância e Adolescência. RESULTADOS: Foram encontradas médias mais altas no Children's Depression Inventory e no Inventário de Eventos Estressores na Infância e Adolescência no grupo institucionalizado, com diferença significativa. Os eventos mais freqüentes foram "ter que obedecer às ordens de seus pais" para o grupo da família e "ser levado para um abrigo" no outro grupo. Os eventos que apresentaram maior impacto foram "morte de um dos pais", "ser estuprado", "ser rejeitado pelos familiares" e "ser tocado sexualmente contra a vontade". Foram encontradas diferenças entre os sexos e entre os contextos no impacto atribuído aos eventos. Os adolescentes, independentemente dos contextos, apresentaram freqüência de eventos significativamente maior do que as crianças, o que não ocorreu quanto ao impacto. CONCLUSÃO: Os jovens abrigados apresentaram maior exposição a situações potenciais de risco, o que pode estar relacionado à maior manifestação de sintomas depressivos. A avaliação do impacto de um evento estressante, no entanto, não esteve correlacionada com o Children's Depression Inventory em nenhum dos contextos.<br>INTRODUCTION: The present study examined the manifestation of depressive symptoms, as well as frequency and impact of stressful events in institutionalized and non-institutionalized children and adolescents. METHOD: A total of 257 youths, of both genders, aged 7-16 years were included in this study. Of these, 130 lived in protection youth shelters and 127 lived with their families in the metropolitan area of Porto Alegre. All participants were attending 1st to 8th grades of public elementary schools located in low-income neighborhoods. Children's Depression Inventory and Stressful Events in Childhood and Adolescence Inventory were used. RESULTS: Data pointed out a significant difference, with higher means in the Children's Depression Inventory and Stressful Events in Childhood and Adolescence Inventory for the institutionalized group. The most frequent events for the family group was "to have to obey your parent's rules," and "to be taken to a protection shelter" was most frequent for the other group. The events of greater impact were "death of one parent," "to be raped," "to be rejected by members of the family," and "to be sexually touched against one's will". Considering the participant's gender and context, differences were found in attributed impact. Adolescents, regardless of context, had a higher frequency of significant events than children, which did not occur concerning impact. CONCLUSION: Results indicated that institutionalized youths were more exposed to potential risk situations, which could be related to the manifestation of more depressive symptoms. However, assessment of the impact of a stressful event was not correlated with the Children's Depression Inventory in any context