Familien von kranken Kindern - Soziale Arbeit im Kontext von Unterstützung und Trauerbegleitung : eine empirische Untersuchung zur Arbeit im Tätigkeitsfeld

Die Bachelorarbeit befasst sich mit Familien von kranken Kindern und deren Begleitung durch die soziale Arbeit. Dabei werden theoretische Inhalte aufgezeigt, welche die Arbeit in diesem Feld betreffen. Im Anschluss wurde mithilfe von Expert*inneninterviews eine Darstellung der Arbeit in Begleitung und Unterstützung der Zielgruppen erstellt.The bachelor thesis deals with families of sick children and their support by social work. Theoretical content is shown that concerns the work in this field. Subsequently, with the help of expert interviews, a presentation of the work was created in the accompaniment and support of the target groups

MALDI-TOF Mass Spectrometry Discriminates Known Species and Marine Environmental Isolates of Pseudoalteromonas

The genus Pseudoalteromonas constitutes an ecologically significant group of marine Gammaproteobacteria with potential biotechnological value as producers of bioactive compounds and of enzymes. Understanding their roles in the environment and bioprospecting for novel products depend on efficient ways of identifying environmental isolates. Matrix Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) biotyping has promise as a rapid and reliable method of identifying and distinguishing between different types of bacteria, but has had relatively limited application to marine bacteria and has not been applied systematically to Pseudoalteromonas. Therefore, we constructed a MALDI-TOF MS database of 31 known Pseudoalteromonas species, to which new isolates can be compared by MALDI-TOF biotyping. The ability of MALDI-TOF MS to distinguish between species was scrutinized by comparison with 16S rRNA gene sequencing. The patterns of similarity given by the two approaches were broadly but not completely consistent. In general, the resolution of MALDI-TOF MS was greater than that of 16S rRNA gene sequencing. The database was tested with 13 environmental Pseudoalteromonas isolates from UK waters. All of the test strains could be identified to genus level by MALDI-TOF MS biotyping, but most could not be definitely identified to species level. We conclude that several of these isolates, and possibly most, represent new species. Thus, further taxonomic investigation of Pseudoalteromonas is needed before MALDI-TOF MS biotyping can be used reliably for species identification. It is, however, a powerful tool for characterizing and distinguishing among environmental isolates and can make an important contribution to taxonomic studies

Satellite monitoring of agricultural land

ATANASOV, Asparuh, DEMIREV, Vladimir, MIHOVA, Galina. Satellite monitoring of agricultural land. In: Development Through Research and Innovation - 2021 [online]: The 2nd International Scientific Conference: Online Conference for Researchers, PhD and Post-Doctoral Students, August 27th, 2021, Chişinău: Conference Proceedings. Chişinău, ASEM, 2021, pp. 16-22. ISBN 978-9975-155-54-0.The object of the present work are the satellite images of agricultural lands, in order to obtain additional information about the development of crops. Farmers cannot afford to process "raw" satellite images, but there are companies available around the world that supply up-to-date images. In addition to images in a wide frequency range, users are offered meteorological information about the terrain, recommendations for fertilizer application and crop treatment, time series and total values of temperatures, precipitation, vegetation indices and the like. The space segment in the collection of data on agricultural crops has become increasingly relevant in recent decades and is an integral part of a comprehensive approach to precision agriculture. The study aims to trace the opportunities and differences in different sources of information. JEL: O13

APPLICATION OF GPS NAVIGATION IN AGRICULTURAL AGGREGATES

A brief analysis has been made about the emergence of the concept of "navigation". The benefits of optimal guidance of agricultural aggregates through GPS navigation are outlined. Some of the positioning accuracy methods that are practiced in agricultural aggregates are analyzed. A poll has been conducted among users of agricultural equipment using GPS navigation. Issue and conclusions are drawn on the benefits and problems for more efficient use of GPS navigation in agriculture

Hydroblack 91 : report of the CTD Intercalibration Workshop

An Intercalibration Workshop was held at the Woods Hole Oceanographic Institution (W.H.O.I.) from 1-10 December, 1991, for the CTD data acquired during HYDROBLACK '91. This intercalibration exercise was a prelude to an interdisciplinary HYDROBLACK '91 intercalibration to be held in Crimea, Ukraine, in February, 1992, incorporating the full suite of physical, biological, and chemical measurements acquired during the cruise. HYDROBLACK '91 acquired for the first time a complete hydrographic, biological, and chemical data set for the entire Black Sea, to 200 m water depth, with the participation of all Black Sea riparian countres as well as the U.S. Nearly 300 hydrographic stations were occupied to full water depth; biological and chemical measurements were made at 100 of these stations. This quasi-synoptic survey was accomplished using five ships during an interval of approximately three weeks. Results show some disparities between CTD's from the different regions, but the intercalibrated results show a consistent and high resolution detail of the dynamic topography and other physical characteristics of the entire Black Sea basin. The intercalibrated data set is now available within each country and from W.H.O.I., and will form the basis for studies on ocean physics as well as interdisciplinary issues such as oxygen depletion within the basin and hydrogen sulfide distribution. This effort provides an intercalibrated, spatially-dense baseline against which all future and past measurements can be compared. In spite of significant economic pressures arising from the changes in the eastern European countries, and the inadequate scientific exchange with the west during the past two decades, HYDROBLACK '91 is considered a success and a model for future international scientific and monitoring efforts thoughout the Black Sea. Similar efforts are anticipated twice-yearly in the framework of the new Cooperative Marine Science Program for the Black Sea.Funding was provided by the National Science Foundation through Grant No. OCE-9121788, the Vetleson Foundation, the Andrew W. Mellon Foundation, the Mobil Foundation, Inc. and the Regional Environmental Center for Central & Eastern Europe (Budapest)

Identification of Tsetse (Glossina spp.) using matrix-assisted laser desorption/ionisation time of flight mass spectrometry

Glossina (G.) spp. (Diptera: Glossinidae), known as tsetse flies, are vectors of African trypanosomes that cause sleeping sickness in humans and nagana in domestic livestock. Knowledge on tsetse distribution and accurate species identification help identify potential vector intervention sites. Morphological species identification of tsetse is challenging and sometimes not accurate. The matrix-assisted laser desorption/ionisation time of flight mass spectrometry (MALDI TOF MS) technique, already standardised for microbial identification, could become a standard method for tsetse fly diagnostics. Therefore, a unique spectra reference database was created for five lab-reared species of riverine-, savannah- and forest- type tsetse flies and incorporated with the commercial Biotyper 3.0 database. The standard formic acid/acetonitrile extraction of male and female whole insects and their body parts (head, thorax, abdomen, wings and legs) was used to obtain the flies' proteins. The computed composite correlation index and cluster analysis revealed the suitability of any tsetse body part for a rapid taxonomical identification. Phyloproteomic analysis revealed that the peak patterns of G. brevipalpis differed greatly from the other tsetse. This outcome was comparable to previous theories that they might be considered as a sister group to other tsetse spp. Freshly extracted samples were found to be matched at the species level. However, sex differentiation proved to be less reliable. Similarly processed samples of the common house fly Musca domestica (Diptera: Muscidae; strain: Lei) did not yield any match with the tsetse reference database. The inclusion of additional strains of morphologically defined wild caught flies of known origin and the availability of large-scale mass spectrometry data could facilitate rapid tsetse species identification in the futur

Detection of Plasmodium Falciparum in Pregnancy by Laser Desorption Mass Spectrometry

Detection of Plasmodium falciparum malaria during pregnancy is complicated by sequestration of parasites in the placenta, which reduces peripheral blood microscopic detection. Laser desorption mass spectrometry (LDMS) has previously demonstrated sensitive detection of hemozoin from P. falciparum blood cultures and the ability to track parasitemia in a Plasmodium yoelii malaria mouse model. Here we use a simple, dilution in water, blood sample preparation protocol for LDMS detection of malaria in 45 asymptomatic, pregnant Zambian women. We compare LDMS to microscopy and polymerase chain reaction (PCR) analysis. All women were microscopy negative. LDMS detected P. falciparum hemozoin in 15 out of 45 women, while PCR results were positive in 25 women. Compared with PCR, which analyzed 20-30 μL of blood, the sensitivity of LDMS, which analyzed \u3c 1 μL of blood, was 52%, with a specificity of 92%. LDMS is a potentially rapid and more sensitive alternate diagnostic method than microscopy. Copyright © 2005 by The American Society of Tropical Medicine and Hygiene

The role of acyl-coenzyme A carboxylase complex in lipstatin biosynthesis of Streptomyces toxytricini

Streptomyces toxytricini produces lipstatin, a specific inhibitor of pancreatic lipase, which is derived from two fatty acid moieties with eight and 14 carbon atoms. The pccB gene locus in 10.6 kb fragment of S. toxytricini chromosomal DNA contains three genes for acyl-coenzyme A carboxylase (ACCase) complex accA3, pccB, and pccE that are presumed to be involved in secondary metabolism. The pccB gene encoding a β subunit of ACCase [carboxyltransferase (CT)] was identified upstream of pccE gene for a small protein of ε subunit. The accA3 encoding the α subunit of ACCase [biotin carboxylase (BC)] was also identified downstream of pccB gene. When the pccB and pccE genes were inactivated by homologous recombination, the lipstatin production was reduced as much as 80%. In contrast, the accumulation of another compound, tetradeca-5.8-dienoic acid (the major lipstatin precursor), was 4.5-fold increased in disruptant compared with wild-type. It implies that PccB of S. toxytricini is involved in the activation of octanoic acid to hexylmalonic acid for lipstatin biosynthesis

Evaluation of Rapid Diagnostics for Plasmodium falciparum and P. vivax in Mae Sot Malaria Endemic Area, Thailand

Prompt and accurate diagnosis of malaria is the key to prevent disease morbidity and mortality. This study was carried out to evaluate diagnostic performance of 3 commercial rapid detection tests (RDTs), i.e., Malaria Antigen Pf/Pan™, Malaria Ag-Pf™, and Malaria Ag-Pv™ tests, in comparison with the microscopic and PCR methods. A total of 460 blood samples microscopically positive for Plasmodium falciparum (211 samples), P. vivax (218), mixed with P. falciparum and P. vivax (30), or P. ovale (1), and 124 samples of healthy subjects or patients with other fever-related infections, were collected. The sensitivities of Malaria Ag-Pf™ and Malaria Antigen Pf/Pan™ compared with the microscopic method for P. falciparum or P. vivax detection were 97.6% and 99.0%, or 98.6% and 99.0%, respectively. The specificities of Malaria Ag-Pf™, Malaria Ag-Pv™, and Malaria Antigen Pf/Pan™ were 93.3%, 98.8%, and 94.4%, respectively. The sensitivities of Malaria Ag-Pf™, Malaria Antigen Pf/Pan™, and microscopic method, when PCR was used as a reference method for P. falciparum or P. vivax detection were 91.8%, 100%, and 96.7%, or 91.9%, 92.6%, and 97.3%, respectively. The specificities of Malaria Ag-Pf™, Malaria Ag-Pv™, Malaria Antigen Pf/Pan™, and microscopic method were 66.2%, 92.7%, 73.9%, and 78.2%, respectively. Results indicated that the diagnostic performances of all the commercial RDTs are satisfactory for application to malaria diagnosis