Financial Analysis of Implementing an Anaerobic Digester and Free Stall Barn System on a Mississippi Dairy Farm

The installation of an anaerobic digestion system and a free stall barn on a Mississippi dairy farm is only feasible with the presence of a cost-share plan. A 60% cost-share plan is the minimum amount of support a farmer must receive in order to install both facilities.Anaerobic digester, Dairy farm, Cost-share plan, Farm Management,

The Bela Bash

There was a meeting recently in Cambridge to celebrate Bollobas’ 60th birthday. There were a number of very good talks presenting many new results and a number of open problems. This talk will be a survey of some of the problems and results that were presented

The role of the systemic inflammatory response, the JAK STAT pathway and the MAPK pathway in the prognosis of resectable pancreatic cancer

Pancreatic cancer is a devastating disease with a five year survival of only 2-3%. Only 10-15% of patients have resectable disease at presentation and the only potential cure is major surgery with adjuvant chemotherapy. The outcomes of surgery are disappointing with a median survival of only15-17 months and operative mortality and morbidity figures of 5-10% and 40% respectively. This abysmal prognosis is likely due to the highly aggressive nature of the tumour, its resistance to adjuvant therapy, its late presentation and the likely presence of micro-metastases not detectable at staging or surgery. A pre-operative systemic inflammatory response (as measured by CRP) is known to be associated with a poor prognosis in a number of cancers including pancreatic cancer. The reasons behind this poor prognosis are not yet known. The main driver of plasma CRP levels is the cytokine IL-6, known to be elevated in the plasma of patients with pancreatic cancer. This thesis hypothesises that upregulation of two IL-6-dependent pathways, the JAK STAT and MAPK pathways is responsible for the poor prognosis associated with an inflammatory response in pancreatic cancer. Both of these pathways are known to be involved in cellular growth, differentiation and apoptosis and when activated they may confer a growth or survival advantage to tumour cells. The aims of this thesis were to establish the prognostic role of a systemic inflammatory response in resectable pancreatic cancer in both a retrospective and prospective cohort and establish whether increased protein expression in either the JAK STAT or MAPK pathways is associated with a poor prognosis in the same retrospective cohort. A retrospective database of 148 patients who had undergone Whipple resection for either pancreatic cancer (PC) or non-pancreatic peri-ampullary cancer (NPPC) was created with pre-operative CRP values and survival data. The author then created tissue micro-arrays (TMA’s) with both tumour and normal pancreatic duct tissue from each of the 148 patients in the retrospective cohort and carried out immunohistochemistry on 12 antibodies known to be crucial in IL-6 signalling (6 in the JAK STAT and 6 in the MAPK pathways). Following staining the author scored each of the antibodies using the weighted histoscore to allow analysis of antigen expression. During the period of research the author also created a prospective database of 36 patients who underwent surgery for either PC or NPPC. Plasma was stored pre-operatively from each of the patients and this was later thawed and using an ELISA kit another research fellow (JL) was able to establish plasma levels of IL-6 in the prospective cohort. On univariate analysis a raised pre-operative CRP was associated with poorer survival, 374 days versus 618 days (p=0.0001) in the retrospective PC group only. On multivariate analysis, only pre-operative CRP retained statistical significance amongst those factors shown to be significant on univariate analysis (P=0.009). In the prospective group, patients with low levels of IL-6 had a median survival of 799 days, against a median survival of 537 days in those with high plasma IL-6 levels (P=0.002) when all 36 patients were analysed together. On analysis of protein expression, no significant relationship between increased expression and poor survival was seen for any of the 12 proteins analysed. The results from this thesis confirm that a pre-operative inflammatory response is associated with poor survival in patients with resectable pancreatic cancer. Raised plasma levels of IL-6 are also associated with poorer survival in similar patients. However, the poor prognosis appears to be via a JAK STAT/ MAPK independent mechanism. Other possible explanations for this poor prognosis including the connection between inflammation and cachexia and other important inflammatory proteins such as NF-κB and SOCS are explored in the discussion of this thesis

Distribution of p63, a novel myoepithelial marker, in fine-needle aspiration biopsies of the breast: an analysis of 82 samples

BACKGROUND. The presence of myoepithelial cells (MECs) in fine-needle aspiration biopsies (FNAB) of the breast constitute an important criterion used to diagnose benign breast lesions. However, MECs sometimes have a distorted cytomorphology, and most of the previously evaluated myoepithelial markers do not have satisfactory sensitivity and specificity. p63, a recently characterized p53 homolog, is a nuclear transcription factor that is expressed in basal cells of multilayered epithelia and myoepithelial cells of the breast. The authors analyzed the immunocytochemical distribution of p63 in a series of 82 breast FNABs (30 benign lesions and 52 malignant breast lesions). METHODS. Eighty-two archival, Papanicolaou-stained smears of breast lesions were retrieved from the files of the authors’ institutions. Immunocytochemistry was performed according to the streptavidin-biotin-peroxidase complex technique using the antibody 4A4 (against all p63 isoforms). Two pathologists evaluated the distribution of p63 positive cells. Only nuclear reactivity was considered specific. RESULTS. In benign lesions, p63 decorated the nuclei of MECs in all samples. p63 also stained naked nuclei in fibroadenomas. In malignant lesions, p63 was positive in MECs overlying malignant cell clusters in all 8 samples of ductal carcinoma in situ (DCIS), in 9 of 16 samples of pure invasive carcinomas (IC), and in 16 of 20 samples that contained both DCIS and IC. In 18 samples (36%), a variable population of p63 positive, malignant cells was observed. p63 failed to decorate stromal, neural, adipocytic, and smooth muscle cells in all samples. CONCLUSIONS. p63 is a reliable nuclear marker of MECs in breast aspirates. Regardless of the fact that variable proportions of p63 positive, malignant cells were observed in 36% of breast carcinoma aspirates, p63 may be a useful adjunct antibody to confirm the presence of MECs in FNABs of benign breast lesions.Fundação para a Ciência e a Tecnologia (FCT) - SFRH/BD/5386/2001

I heard it through the grapevine : A Randomized Controlled Trial on the Direct and Vicarious Effects of Preventative Specific Deterrence Initiatives in Criminal Networks

A rich body of literature exists on deterrence, yet little is known about how deterrence messages are communicated through social networks. This is an important gap in our understanding, because such communication gives rise to the possibility that social institutions can utilize the vicarious effect of the threat of punishment against one individual to reduce the rate of reoffending amongst their criminal associates. To test this, we identified criminals with an extensive offending history (prolific offenders) and their co-offenders using social network analysis and then conducted a randomized controlled trial to measure the effect on both prolific offenders and their cooffenders of delivering a “specific deterrence” message. The treatment— preemptive engagements with prolific offenders by a police officer offering both ‘carrots’ (desistance pathways) and ‘sticks’ (increased sanction threat)—was applied to the prolific offenders, but not to their co-offenders. The outcomes suggest that a single officer–offender engagement leads to a crime suppression effect in all comparisons, with 21.3%, 11.0%, and 15.0% reductions for specific, vicarious, and total network deterrence effects, respectively. The findings suggest that (a) social network analysis based on in-house police records can be used to cartographically understand social networks of offenders, with an aim of preventing crime; (b) deterrence messages promulgated by the police have the capacity to reduce crime beyond what was previously assumed, as the cascading of threats in cooffending relationships carries a vicarious crime reduction impact; (c) unlike “reactive specific deterrence” (i.e., a threat of punishment following a specific and detected crime) which can have perverse effects on certain offenders, preventative specific deterrence is a promising crime policy

"I heard it through the grapevine": A Randomized Controlled Trial on the Direct and Vicarious Effects of Preventative Specific Deterrence Initiatives in Criminal Networks

A rich body of literature exists on deterrence, yet little is known about how deterrence messages are communicated through social networks. This is an important gap in our understanding, because such communication gives rise to the possibility that social institutions can utilize the vicarious effect of the threat of punishment against one individual to reduce the rate of reoffending amongst their criminal associates. To test this, we identified criminals with an extensive offending history (“prolific offenders”) and their co-offenders using social network analysis and then conducted a randomized controlled trial to measure the effect on both prolific offenders and their co-offenders of delivering a “specific deterrence” message. The treatment—preemptive engagements with prolific offenders by a police officer offering both ‘carrots’ (desistance pathways) and ‘sticks’ (increased sanction threat)—was applied to the prolific offenders, but not to their co-offenders. The outcomes suggest that a single officer–offender engagement leads to a crime suppression effect in all comparisons, with 21.3%, 11.0%, and 15.0% reductions for specific, vicarious, and total network deterrence effects, respectively. The findings suggest that (a) social network analysis based on in-house police records can be used to prevent crime; (b) deterrence messages promulgated by the police have the capacity to reduce crime beyond what was previously assumed, as the cascading of threats in co-offending relationships carries a vicarious crime reduction impact; (c) unlike “reactive specific deterrence” (i.e., a threat of punishment following a specific and detected crime) which can have perverse effects on certain offenders, preventative specific deterrence is a promising crime policy.N/

‘Eke out our performance with your mind’: Teaching Shakespeare in the New Millennium

There are hundreds of How-to-Teach-Shakespeare books flooding the market, and more tomes pile up annually. Some are geared to elementary and high school teachers, others to teachers of literature and drama at tertiary level, and of course many too are aimed at current performers of Shakespeare. Having directed or performed about half of Shakespeare’s canon, as well as having created many anthology plays based on his work, I am constantly on the lookout for new ways to bring life to Shakespeare. I have also been privileged to work at London’s rebuilt Globe Theatre. Although the experience of playing Shakespeare outdoors is not new, that of playing in this reconstructed space is, and lessons from that experience cannot help but have affected all my subsequent work and readings. This article will examine five recent texts that, with varying levels of success, discuss varying approaches to opening up his works. The scope is wide and the authors aim to assist both teachers and students of Shakespeare, whether from a practical hands-on approach to the text or through an analytical theoretical approach