Biomarker Exploration in Human Peripheral Blood Mononuclear Cells for Monitoring Sulforaphane Treatment Responses in Autism Spectrum Disorder

Autism Spectrum Disorder (ASD) is one of the most common neurodevelopmental disorders with no drugs treating the core symptoms and no validated biomarkers for clinical use. The multi-functional phytochemical sulforaphane affects many of the biochemical abnormalities associated with ASD. We investigated potential molecular markers from three ASD-associated physiological pathways that can be affected by sulforaphane: redox metabolism/oxidative stress; heat shock response; and immune dysregulation/inflammation, in peripheral blood mononuclear cells (PBMCs) from healthy donors and patients with ASD. We first analyzed the mRNA levels of selected molecular markers in response to sulforaphane ex vivo treatment in PBMCs from healthy donors by real-time quantitative PCR. All of the tested markers showed quantifiability, accuracy and reproducibility. We then compared the expression levels of those markers in PBMCs taken from ASD patients in response to orally-delivered sulforaphane. The mRNA levels of cytoprotective enzymes (NQO1, HO-1, AKR1C1), and heat shock proteins (HSP27 and HSP70), increased. Conversely, mRNA levels of pro-inflammatory markers (IL-6, IL-1beta, COX-2 and TNF-alpha) decreased. Individually none is sufficiently specific or sensitive, but when grouped by function as two panels, these biomarkers show promise for monitoring pharmacodynamic responses to sulforaphane in both healthy and autistic humans, and providing guidance for biomedical interventions

Characterising a solid state qubit via environmental noise

We propose a method for characterising the energy level structure of a solid-state qubit by monitoring the noise level in its environment. We consider a model persistent-current qubit in a lossy resevoir and demonstrate that the noise in a classical bias field is a sensitive function of the applied field.Comment: 3 Figure

Near-Surface Imaging of a Hydrogeothermal System at Mount Princeton, Colorado Using 3D Seismic, Self-Potential, and DC Resistivity Data

The Upper Arkansas Valley in the Rocky Mountains of central Colorado is the northernmost extensional basin of the Rio Grande Rift(Figure 1). The valley is a half graben bordered to the east and west by the Mosquito and Sawatch ranges,respectively. The Sawatch Range is home to the Collegiate Peaks,which include some of the highest summits in the Rocky Mountains. Some Collegiate Peaks over 4250 m (14,000 ft) from north to south include Mount Harvard, Mount Yale, Mount Princeton,and Mount Antero. The Sawatch range-front normal fault strikes north-northwest along the eastern margin of the Collegiate Peaks and is characterized by a right-lateral offset between the Mount Princeton batholith and Mount Antero. This offset in basin-bounding faults is accommodated by a northeast-southwest dextral strike-slip transfer fault (Richards et al.,2010) and coincides with an area of hydrogeothermal activity and Mount Princeton Hot Springs. This transfer fault is here termed the Chalk Creek fault due to it\u27s alignment with the Chalk Creek valley. A 250-m high erosional scarp, called the Chalk Cliffs, lies along the northern boundary of this valley.The cliffs are geothermally altered quartz monzonite and not chalk.These cliffs coincide with the Chalk Creek fault, whose intersection with the Sawatch range-front normal fault results in a primary pathway for upwelling geothermal waters

Clinical academic career pathway for nursing and allied health professionals: clinical academic role descriptors

The clinical academic pathway outlined highlights the range of typical practice and research-focused activities that a practitioner on a clinical academic career pathway might normally engage in at different levels and points along this career path. The activities are intended as a guide for practitioners interested in learning more about the practice and research components of a clinical academic career, as well as those already employed in clinical academic roles. They may also be useful for health care organisations and Higher Education Institutions as a tool for developing clinical academic roles

Conservative Management Of A Massive Rotator Cuff Tear And Partial Tear Of The Long Head Of The Biceps: A Case Report

A rotator cuff tear (RCT) is a common injury to the shoulder musculature that increases in prevalence with age. RCT’s can be classified into five categories determined by the muscular involved: Type A: supraspinatus & superior subscapularis; Type B: supraspinatus & entire subscapularis; Type C: supraspinatus, superior subscapularis & infraspinatus; Type D: supraspinatus & infraspinatus tears; Type E: supraspinatus,infraspinatus & teres minor. Massive RCT is classified as \u3e5cm in size, or complete tear of two or more tendons. Some massive tears may be considered irreparable due to the extent of tendon retraction, atrophy, arthritis and mobilization. Current literature supports the use of physical therapy (PT) as the primary conservative treatment method for irreparable RCTs. The purpose of this case report was to establish a comprehensive physical therapy plan of care in order to improve the shoulder function of a patient with a non-traumatic, massive RCT, with subsequent partial tear of the long head of the biceps.https://dune.une.edu/pt_studcrposter/1179/thumbnail.jp

The Patent-Antitrust Problem

The Patent-Antitrust section of the Report of the Attorney General\u27s National Committee to Study the Antitrust Laws is an excellent analysis of the existing law and is an invaluable handbook for practitioners in this difficult field. The writer\u27s approach to the problem is different from that of the committee and before commenting specifically on the Report it is only fair to state the writer\u27s views of the problem lest differences in viewpoint give the impression of criticism of the Report. In any approach to the patent-antitrust problem there is a basic question: does a patent-antitrust problem exist? The Report of the Attorney General\u27s Committee indicates that there is such a problem and that the problem involves many ramifications which are difficult of solution. The writer believes that there is no such problem