Cytoplasmic DNA in the unfertilized sea urchin egg: Physical properties of circular mitochondrial DNA and the occurrence of catenated forms

The mitochondrial DNA in the unfertilized egg of the sea urchin Lytechinus pictus is present in an amount approximately seven times that of the haploid nuclear DNA.(1) The mitochondrial DNA has a higher buoyant density than the nuclear DNA and consists of circular duplex molecules of a uniform size of about 5µ. The circular DNA has been recovered(1) in both the intact (closed) and nicked (open) states characteristic of the circular duplex viral DNA's(2) and the mitochondrial DNA's from birds and mammals.(3, 4

Estimating the Impacts of Storage Dry Matter Losses on Switchgrass Production

This poster estimates dry matter losses as a function of harvest method, storage treatment, and time in storage. We then calculate the cost to store switchgrass bales under alternate harvest method and storage treatment scenarios; and determine the breakeven harvest method and storage treatment as a function of biomass price and time in storage.Biomass, bioenergy crops, function form, sustainable systems, Farm Management, Production Economics, Q10, Q42,

VARIABLE RATE NITROGEN APPLICATION ON CORN FIELDS: THE ROLE OF SPATIAL VARIABILITY AND WEATHER

Meta-response functions for corn yields and nitrogen losses were estimated from EPIC-generated data for three soil types and three weather scenarios. These metamodels were used to evaluate variable rate (VRT) versus uniform rate (URT) nitrogen application technologies for alternative weather scenarios and policy option. Except under very dry conditions, returns per acre for VRT were higher than for URT and the economic advantage of VRT increased as realized rainfall decreased from expected average rainfall. Nitrogen losses to the environment from VRT were lower for all situation examined, except on fields with little spatial variability.Corn, environment, meta-response functions, nitrogen restriction, precision farming, site-specific management, spatial variability, weather variability, Crop Production/Industries,

Economic Analysis of the Effects of Winter Cover Crops on No-Tillage Corn Yield Response to Fertilizer Nitrogen

Crop Production/Industries,

Reevaluating the Management of Chronic Temporomandibular Pain Are We Treating PTSD with Debridement and Lavage?

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High-entropy high-hardness metal carbides discovered by entropy descriptors

High-entropy materials have attracted considerable interest due to the combination of useful properties and promising applications. Predicting their formation remains the major hindrance to the discovery of new systems. Here we propose a descriptor - entropy forming ability - for addressing synthesizability from first principles. The formalism, based on the energy distribution spectrum of randomized calculations, captures the accessibility of equally-sampled states near the ground state and quantifies configurational disorder capable of stabilizing high-entropy homogeneous phases. The methodology is applied to disordered refractory 5-metal carbides - promising candidates for high-hardness applications. The descriptor correctly predicts the ease with which compositions can be experimentally synthesized as rock-salt high-entropy homogeneous phases, validating the ansatz, and in some cases, going beyond intuition. Several of these materials exhibit hardness up to 50% higher than rule of mixtures estimations. The entropy descriptor method has the potential to accelerate the search for high-entropy systems by rationally combining first principles with experimental synthesis and characterization.Comment: 12 pages, 2 figure

Is Switchgrass Yield Response to Nitrogen Fertilizer Dynamic? Implications for Profitability and Sustainability at the Farm Level

Revised version of the paper submitted 2/11/2010Biomass, Energy Crops, Sequential Inputs, West Tennessee, Crop Production/Industries, Farm Management, Production Economics, Resource /Energy Economics and Policy,

Switchgrass Production in Marginal Environments: A Comparative Economic Analysis across Four West Tennessee Landscapes

Switchgrass (Panicum virgatum L.) has been identified as a model feedstock for the emerging biofuels industry. Its selection was based, in part, upon the observation that switchgrass can produce high yields in marginal production environments. This trait may become particularly valuable in coming years, as renewable fuel mandates begin to take effect and concerns over the food-versus-fuel debate increase. Relatively little research information exists about how management practices and production costs vary across different production environments. The objectives of this research were (a) to compare switchgrass yields as influenced by seeding rate and nitrogen fertilization rates in low-, intermediate-, and high-yielding switchgrass production environments, (b) to determine the economically optimal seeding rate and nitrogen fertilization rate for each environment, and (c) to calculate per-ton production costs. Experimental yield data from four locations were utilized for this study. Plots were seeded in 2004 with treatments of 2.5, 5.0, 7.5, 10.0, and 12.5 lbs/acre. Nitrogen was applied in subsequent intervals at 0, 60, 120 and 180 lbs/acre. For an expected stand lifespan of 10 years, production costs ranged from $45 per ton in a well drained level upland environment ideal for the production of row crops to$70 per ton in a marginal, poorly drained flood plain in which the switchgrass stand was slow to establish and which demonstrated lower overall yields.Crop Production/Industries, International Relations/Trade,

Inhibition of adenovirus serotype 14 infection by octadecyloxyethyl esters of (S)-[(3-hydroxy-2-phosphonomethoxy)propyl]- nucleosides in vitro.

On September 22, 2008, a physician on Prince of Wales Island, Alaska, notified the Alaska Department of Health and Social Services (ADHSS) of an unusually high number of adult patients with recently diagnosed pneumonia (n = 10), including three persons who required hospitalization and one who died. ADHSS and CDC conducted an investigation to determine the cause and distribution of the outbreak, identify risk factors for hospitalization, and implement control measures. This report summarizes the results of that investigation, which found that the outbreak was caused by adenovirus 14 (Ad14), an emerging adenovirus serotype in the United States that is associated with a higher rate of severe illness compared with other adenoviruses. Among the 46 cases identified in the outbreak from September 1 through October 27, 2008, the most frequently observed characteristics included the following: male (70%), Alaska Native (61%), underlying pulmonary disease (44%), aged > or = 65 years (26%), and current smoker (48%). Patients aged > or = 65 years had a fivefold increased risk for hospitalization. The most commonly reported symptoms were cough (100%), shortness of breath (87%), and fever (74%). Of the 11 hospitalized patients, three required intensive care, and one required mechanical ventilation. One death was reported. Ad14 isolates obtained during the outbreak were identical genetically to those in recent community-acquired outbreaks in the United States which suggests the emergence of a new, and possibly more virulent Ad14 variant. Clinicians should consider Ad14 infection in the differential diagnosis for patients with community-acquired pneumonia, particularly when unexplained clusters of severe respiratory infections are detected