Colour reverse learning and animal personalities: the advantage of behavioural diversity assessed with agent-based simulations

Foraging bees use colour cues to help identify rewarding from unrewarding flowers, but as conditions change, bees may require behavioural flexibility to reverse their learnt preferences. Perceptually similar colours are learnt slowly by honeybees and thus potentially pose a difficult task to reverse-learn. Free-flying honeybees (N = 32) were trained to learn a fine colour discrimination task that could be resolved at ca. 70% accuracy following extended differential conditioning, and were then tested for their ability to reverse-learn this visual problem multiple times. Subsequent analyses identified three different strategies: ‘Deliberative-decisive’ bees that could, after several flower visits, decisively make a large change to learnt preferences; ‘Fickle- circumspect’ bees that changed their preferences by a small amount every time they encountered evidence in their environment; and ‘Stay’ bees that did not change from their initially learnt preference. The next aim was to determine if there was any advantage to a colony in maintaining bees with a variety of decision-making strategies. To understand the potential benefits of the observed behavioural diversity agent-based computer simulations were conducted by systematically varying parameters for flower reward switch oscillation frequency, flower handling time, and fraction of defective ‘target’ stimuli. These simulations revealed that when there is a relatively high frequency of reward reversals, fickle-circumspect bees are more efficient at nectar collection. However, as the reward reversal frequency decreases the performance of deliberative-decisive bees becomes most efficient. These findings show there to be an evolutionary benefit for honeybee colonies with individuals exhibiting these different strategies for managing resource change. The strategies have similarities to some complex decision-making processes observed in humans, and algorithms implemented in artificial intelligence systems

Kondo effect and anti-ferromagnetic correlation in transport through tunneling-coupled double quantum dots

We propose to study the transport through tunneling-coupled double quantum dots (DQDs) connected in series to leads, using the finite-$U$ slave-boson mean field approach developed initially by Kotliar and Ruckenstein [Phys. Rev. Lett. {\bf 57}, 1362 (1986)]. This approach treats the dot-lead coupling and the inter-dot tunnelling $t$ nonperturbatively at arbitrary Coulomb correlation $U$, thus allows the anti-ferromagnetic exchange coupling parameter $J=4t^2/U$ to appear naturally. We find that, with increasing the inter-dot hopping, the DQDs manifest three distinct physical scenarios: the Kondo singlet state of each dot with its adjacent lead, the spin singlet state consisting of local spins on each dot and the doubly occupied bonding orbital of the coupled dots. The three states exhibit remarkably distinct behavior in transmission spectrum, linear and differential conductance and their magnetic-field dependence. Theoretical predictions agree with numerical renormalization group and Lanczos calculations, and some of them have been observed in recent experiments.Comment: 5 pages, 5 figures. Physics Review B (Rapid Communication) (in press

Involvement of Plasmodium falciparum protein kinase CK2 in the chromatin assembly pathway

<p>Abstract</p> <p>Background</p> <p>Protein kinase CK2 is a pleiotropic serine/threonine protein kinase with hundreds of reported substrates, and plays an important role in a number of cellular processes. The cellular functions of <it>Plasmodium falciparum </it>CK2 (PfCK2) are unknown. The parasite's genome encodes one catalytic subunit, PfCK2α, which we have previously shown to be essential for completion of the asexual erythrocytic cycle, and two putative regulatory subunits, PfCK2β1 and PfCK2β2.</p> <p>Results</p> <p>We now show that the genes encoding both regulatory PfCK2 subunits (PfCK2β1 and PfCK2β2) cannot be disrupted. Using immunofluorescence and electron microscopy, we examined the intra-erythrocytic stages of transgenic parasite lines expressing hemagglutinin (HA)-tagged catalytic and regulatory subunits (HA-CK2α, HA-PfCK2β1 or HA-PfCK2β2), and localized all three subunits to both cytoplasmic and nuclear compartments of the parasite. The same transgenic parasite lines were used to purify PfCK2β1- and PfCK2β2-containing complexes, which were analyzed by mass spectrometry. The recovered proteins were unevenly distributed between various pathways, with a large proportion of components of the chromatin assembly pathway being present in both PfCK2β1 and PfCK2β2 precipitates, implicating PfCK2 in chromatin dynamics. We also found that chromatin-related substrates such as nucleosome assembly proteins (Naps), histones, and two members of the Alba family are phosphorylated by PfCK2α <it>in vitro</it>.</p> <p>Conclusions</p> <p>Our reverse-genetics data show that each of the two regulatory PfCK2 subunits is required for completion of the asexual erythrocytic cycle. Our interactome study points to an implication of PfCK2 in many cellular pathways, with chromatin dynamics being identified as a major process regulated by PfCK2. This study paves the way for a kinome-wide interactomics-based approach to elucidate protein kinase function in malaria parasites.</p

Dimensional Crossover of Weak Localization in a Magnetic Field

We study the dimensional crossover of weak localization in strongly anisotropic systems. This crossover from three-dimensional behavior to an effective lower dimensional system is triggered by increasing temperature if the phase coherence length gets shorter than the lattice spacing $a$. A similar effect occurs in a magnetic field if the magnetic length $L_m$ becomes shorter than $a(D_{||}/D_\perp)^\gamma$, where \D_{||}/D_\perp is the ratio of the diffusion coefficients parallel and perpendicular to the planes or chains. $\gamma$ depends on the direction of the magnetic field, e.g. $\gamma=1/4$ or 1/2 for a magnetic field parallel or perpendicular to the planes in a quasi two-dimensional system. We show that even in the limit of large magnetic field, weak localization is not fully suppressed in a lattice system. Experimental implications are discussed in detail.Comment: RevTeX, 11 pages, 4 figures; three references added and discusse

Plasma Resonance in Layered Normal Metals and Superconductors

A microscopic theory of the plasma resonance in layered metals is presented. It is shown that electron-impurity scattering can suppress the plasma resonance in the normal state and sharpen it in the superconducting state. Analytic properties of the conductivity for the electronic transport perpendicular to the layers are investigated. The dissipative part of the electromagnetic response in c-direction has been found to depend on frequency in a highly non-trivial manner. This sort of behavior cannot be incorporated in the widely used phenomenological Gorter-Kazimir model.Comment: 34 pages including 12 figures in uuencoded.file. A revised version. Several formulas and a number of misprints are corrected. A problem with printing of figures is fixe

The Metal-Insulator Transition in the Doubly Degenerate Hubbard Model

A systematic study has been made on the metal-insulator (MI) transition of the doubly degenerate Hubbard model (DHM) in the paramagnetic ground state, by using the slave-boson mean-field theory which is equivalent to the Gutzwiller approximation (GA). For the case of infinite electron-electron interactions, we obtain the analytic solution, which becomes exact in the limit of infinite spatial dimension. On the contrary, the finite-interaction case is investigated by numerical methods with the use of the simple-cubic model with the nearest-neighbor hopping. The mass-enhancement factor, $Z$, is shown to increase divergently as one approaches the integer fillings ($N = 1, 2, 3$), at which the MI transition takes place, $N$ being the total number of electrons. The calculated $N$ dependence of $Z$ is compared with the observed specific-heat coefficient, $\gamma$, of $Sr_{1-x}La_xTiO_3$ which is reported to significantly increase as $x$ approaches unity.Comment: Latex 16 pages, 10 ps figures included, published in J. Phys. Soc. Jpn. with some minor modifications. ([email protected]

NRG Oncology-Radiation Therapy Oncology Group Study 1014: 1-Year Toxicity Report From a Phase 2 Study of Repeat Breast-Preserving Surgery and 3-Dimensional Conformal Partial-Breast Reirradiation for In-Breast Recurrence.

PURPOSE: To determine the associated toxicity, tolerance, and safety of partial-breast reirradiation. METHODS AND MATERIALS: Eligibility criteria included in-breast recurrence occurring \u3e1 year after whole-breast irradiation, \u3c3 \u3ecm, unifocal, and resected with negative margins. Partial-breast reirradiation was targeted to the surgical cavity plus 1.5 cm; a prescription dose of 45 Gy in 1.5 Gy twice daily for 30 treatments was used. The primary objective was to evaluate the rate of grade ≥3 treatment-related skin, fibrosis, and/or breast pain adverse events (AEs), occurring ≤1 year from re-treatment completion. A rate of ≥13% for these AEs in a cohort of 55 patients was determined to be unacceptable (86% power, 1-sided α = 0.07). RESULTS: Between 2010 and 2013, 65 patients were accrued, and the first 55 eligible and with 1 year follow-up were analyzed. Median age was 68 years. Twenty-two patients had ductal carcinoma in situ, and 33 had invasive disease: 19 ≤1 cm, 13 \u3e1 to ≤2 cm, and 1 \u3e2 cm. All patients were clinically node negative. Systemic therapy was delivered in 51%. All treatment plans underwent quality review for contouring accuracy and dosimetric compliance. All treatment plans scored acceptable for tumor volume contouring and tumor volume dose-volume analysis. Only 4 (7%) scored unacceptable for organs at risk contouring and organs at risk dose-volume analysis. Treatment-related skin, fibrosis, and/or breast pain AEs were recorded as grade 1 in 64% and grade 2 in 7%, with only 1 ( CONCLUSION: Partial-breast reirradiation with 3-dimensional conformal radiation therapy after second lumpectomy for patients experiencing in-breast failures after whole-breast irradiation is safe and feasible, with acceptable treatment quality achieved. Skin, fibrosis, and breast pain toxicity was acceptable, and grade 3 toxicity was rare