Imprint cytology of osteosarcoma of the jaw: a case report

Introduction. Osteosarcomas are highly malignant bone-forming neoplasms that account for about 20% of all sarcomas. In light of their aggressive behavior, early diagnosis is crucial for determining adequate treatment. Dental professionals may be the first to detect jaw osteosarcomas in their initial stages. The aim of this case report is to draw attention to the possibility of diagnosing this tumor based on clinical, radiographical and cytological characteristics before confirmation by histology. Case presentation. A 24-year-old Afro-Brazilian man presented with swelling and pain on the left side of the mandible in the region of the third molar (tooth 38). Radiography showed a poorly delimited intraosseous lesion with radiolucent and radiopaque areas. The cytological aspects were consistent with the diagnosis of osteosarcoma, which was confirmed by biopsy. Conclusion. Imprint cytology was found to be a reliable, rapid and easy complementary examination. An early diagnosis of osteosarcoma of the jaw is fundamental to the early determination of an adequate treatment. © 2009 Cabral et al; licensee BioMed Central Ltd

A uniform phase representation for the harmonic model in speech synthesis applications

Feature-based vocoders, e.g., STRAIGHT, offer a way to manipulate the perceived characteristics of the speech signal in speech transformation and synthesis. For the harmonic model, which provide excellent perceived quality, features for the amplitude parameters already exist (e.g., Line Spectral Frequencies (LSF), Mel-Frequency Cepstral Coefficients (MFCC)). However, because of the wrapping of the phase parameters, phase features are more difficult to design. To randomize the phase of the harmonic model during synthesis, a voicing feature is commonly used, which distinguishes voiced and unvoiced segments. However, voice production allows smooth transitions between voiced/unvoiced states which makes voicing segmentation sometimes tricky to estimate. In this article, two-phase features are suggested to represent the phase of the harmonic model in a uniform way, without voicing decision. The synthesis quality of the resulting vocoder has been evaluated, using subjective listening tests, in the context of resynthesis, pitch scaling, and Hidden Markov Model (HMM)-based synthesis. The experiments show that the suggested signal model is comparable to STRAIGHT or even better in some scenarios. They also reveal some limitations of the harmonic framework itself in the case of high fundamental frequencies.G. Degottex has been funded by the Swiss National Science Foundation (SNSF) (grants PBSKP2_134325, PBSKP2_140021), Switzerland, and the Foundation for Research and Technology-Hellas (FORTH), Heraklion, Greece. D. Erro has been funded by the Basque Government (BER2TEK, IE12-333) and the Spanish Ministry of Economy and Competitiveness (SpeechTech4All, TEC2012-38939-C03-03)

Risky sexual practices among men who have sex with men in Northeast Brazil: results from four sequential surveys Práticas sexuais de risco de homens que fazem sexo com homens no Nordeste do Brasil: resultados de quatro inquéritos seqüenciais

This paper focuses on recent trends in risky sexual practices for HIV/AIDS among men who have sex with men (MSM) in Fortaleza, Ceará State, Brazil. Four cross-sectional surveys were conducted (1995, 1998, 2002, and 2005) among MSM 14 years or older who reported oral or anal sex in the previous 12 months. Sexual practices were considered risky whenever the respondent reported unprotected receptive or insertive anal intercourse in the six months preceding the interview. Different selection techniques were used to recruit the study population: snowball (1995, 1998, 2002 - 32%); time-space sampling (2002 - 68%); and respondent-driven sampling (2005). Analyses were based on the comparison between proportions. High rates of risky sexual practices were reported in 1995 (49.9%), decreasing in 1998 (32.6%), increasing again in 2002 (51.3%), and showing the lowest level in 2005 (31.4%). Participants with more schooling increased their risky practices from 1998 to 2002, decreasing in 2005. Among individuals with medium or low schooling, risky behavior declined from 2002 to 2005. The article highlights the need for behavioral surveillance to properly address STD/HIV prevention.<br>Estudaram-se as tendências recentes das práticas sexuais de risco para DST/AIDS entre homens que fazem sexo com homens (HSH) em Fortaleza, Ceará, Brasil. Realizaram-se quatro estudos seccionais (1995, 1998, 2002 e 2005) em HSH, com 14 anos ou mais, que tenham referido prática sexual oral ou anal com homens nos últimos 12 meses. Definiu-se prática sexual de risco uma relação anal insertiva ou receptiva sem uso de preservativo nos seis meses que antecederam a entrevista. Utilizaram-se técnicas de recrutamento do tipo snow ball (1995, 1998, 2002 - 32%); time space sampling (2002 - 68%) e respondent driven sampling (2005). Análises basearam-se nas comparações entre proporções. Elevados percentuais de práticas sexuais de risco foram referidos em 1995 (49,9%), decrescendo em 1998 (32,6%), crescendo em 2002 (51,3%) e apresentando os menores percentuais em 2005 (31,4%). Indivíduos com escolaridade mais elevada aumentaram as práticas de risco entre 1998 e 2002, decrescendo no último período. Nos de estrato de escolaridade baixa ou média observou-se declínio no comportamento de risco entre 2002 e 2005. Os resultados deste estudo salientam a importância da vigilância comportamental para adequação dos programas de prevenção das DST/AIDS nessa população

A fluorescent membrane tension probe

Cells and organelles are delimited by lipid bilayers in which high deformability is essential to many cell processes, including motility, endocytosis and cell division. Membrane tension is therefore a major regulator of the cell processes that remodel membranes, albeit one that is very hard to measure in vivo. Here we show that a planarizable push-pull fluorescent probe called FliptR (fluorescent lipid tension reporter) can monitor changes in membrane tension by changing its fluorescence lifetime as a function of the twist between its fluorescent groups. The fluorescence lifetime depends linearly on membrane tension within cells, enabling an easy quantification of membrane tension by fluorescence lifetime imaging microscopy. We further show, using model membranes, that this linear dependency between lifetime of the probe and membrane tension relies on a membrane-tension-dependent lipid phase separation. We also provide calibration curves that enable accurate measurement of membrane tension using fluorescence lifetime imaging microscopy