Single-cell RNA Sequencing (scRNA-seq) Exploratory Analyses to Investigate Aging-dependent Changes and Regional Heterogeneity in Glial Cells

My thesis project research consisted of single-cell RNA-sequencing and bioinformatics using R programming and Neuroscience Multi-omic Data Archive (NeMO) Analytics to examine the impact of aging, brain region, and other variables on the genotypes and phenotypes of oligodendrocytes (OLGs) and oligodendrocyte precursor cells (OPCs). The overarching goal was to increase understanding of glial cell fate and function. After determining bioinformatical findings, follow-up wet-bench experiments were performed to validate results. RNA in situ hybridization and immunohistochemistry were performed to confirm differential gene expression across cell types and brain regions. These experiments also revealed any changes in oligodendrocyte (OLG) development and maturation with the loss of the gene of interest (Choi et al., 2018). The conclusions impact the broader neurobioinformatics field, specifically revolving around the relationship between neural-glial cell interactions and neurodegenerative diseases

The Zero Suicide Program (ZSP) as a Strategy for Reduction and Prevention of Suicides Among Active Duty United States Air Force Members: A Program and Evaluation Plan

The problem of suicide among our military members is one of growing concern for military commanders and political leaders alike. Traditionally the national suicide rates have usually remained higher than that of the military at about 20 per 100,000. Depending on whose calculations to believe though, it appears that the rate of rise in suicides is much higher in military members than among their civilian counterparts. Although not as high as the United States Army (USA), rates among members of the United States Air Force (USAF) have also been on the rise. The only formal program instituted by the USAF to counteract suicides is the USAF Suicide Prevention Program (SPP) launched over 18 years ago in 1996. Although it may have had a positive effect in the years immediately following its launch, recent figures both published and unpublished suggest that effect no longer seems to be present. This paper begins with a review of existing suicide prevention programs by first outlining the results of a systematic review of the published literature. A proposed Zero Suicides Program (ZSP) model to update and improve the current existing SPP, will then be introduced. This will be accomplished with a focus on causal factors possibly unique to the USAF, by describing its relevant theory basis, goals and objectives, implementation, logic model and evaluation plan. Implementation of the ZSP will be accomplished by meeting a series of short and long term objectives, with an eventual goal of wide spread dissemination to the Air Force and military wide community.Master of Public Healt

Olivier Devillers (éd.), Autour de Pline le Jeune. En hommage à Nicole Méthy

Cet ouvrage, publié en hommage à N. Méthy, a été l’occasion pour ses col­lègues de l’Université de Bordeaux 3 de recueillir différentes études (dont quatre de N. Méthy, parmi lesquelles un inédit qui ouvre le volume) sur l’un de ses sujets de prédilection, Pline le Jeune. Plusieurs contributions permettent, par ailleurs, d’évoquer le contexte historique et cultu­rel au début des Antonins. Vingt textes ont ainsi été répartis en trois parties : la pre­mière traite du contexte politique et idéo­..

Pd-catalyzed sp-sp³cross-coupling of benzyl bromides using lithium acetylides

Organolithium-based cross-coupling reactions have emerged as an indispensable method to construct C-C bonds. These transformations have proven particularly useful for the direct and fast coupling of various organolithium reagents (sp, sp2, and sp3) with aromatic (pseudo) halides (sp2). Here we present an efficient method for the cross-coupling of benzyl bromides (sp3) with lithium acetylides (sp). The reaction proceeds within 10 min at room temperature and can be performed in the presence of organolithium-sensitive functional groups such as esters, nitriles, amides and boronic esters. The potential application of the methodology is demonstrated in the preparation of key intermediates used in pharmaceuticals, chemical biology and natural products