Some personal and historical notes on the utility of deep-etch electron microscopy for making cell structure/function correlations

This brief essay talks up the advantages of metal replicas for electron microscopy and explains why they are still the best way to image frozen cells in the electron microscope. Then it explains our approach to freezing, namely the Van Harreveld trick of “slamming” living cells onto a supercold block of metal sprayed with liquid helium at −269ºC, and further talks up this slamming over the alternative of high-pressure freezing, which is much trickier but enjoys greater favor at the moment. This leads me to bemoan the fact that there are not more young investigators today who want to get their hands on electron microscopes and use our approach to get the most “true to life” views of cells out of them with a minimum of hassle. Finally, it ends with a few perspectives on my own career and concludes that, personally, I'm permanently stuck with the view of the “founding fathers” that cell ultrastructure will ultimately display and explain all of cell function, or as Palade said in his Nobel lecture,electron micrographs are “irresistible and half transparent … their meaning buried under only a few years of work,” and “reasonable working hypotheses are already suggested by the ultrastructural organization itself.

Analytical study of the optimum geometric configuration of a space shuttle materials laboratory

A steady state, collisionless flow analysis was made of the density distribution within a hemisphere-disc system due to independent, uniformly distributed internal gas sources. The model was used to estimate the density within a molecular shield, deployed from the shuttle orbiter, which contained internal experiments having a prescribed gas source. Contour plots of the density distribution within the system were presented for disc-to-hemisphere radius ratios of .1, .3, .5, .7, and for disc-to-hemisphere surface emission flux density ratios of .01, 1, 100. The hemisphere-disc system was compared to the empty hemisphere, and it was found that if the disc emission flux density was the same as the hemisphere and the disc radius was not greater than 1/3 of the hemisphere radius, the increase in density at the center of the hemisphere-disc system was less than 50%

Field-tuned quantum critical point of antiferromagnetic metals

A magnetic field applied to a three-dimensional antiferromagnetic metal can destroy the long-range order and thereby induce a quantum critical point. Such field-induced quantum critical behavior is the focus of many recent experiments. We investigate theoretically the quantum critical behavior of clean antiferromagnetic metals subject to a static, spatially uniform external magnetic field. The external field does not only suppress (or induce in some systems) antiferromagnetism but also influences the dynamics of the order parameter by inducing spin precession. This leads to an exactly marginal correction to spin-fluctuation theory. We investigate how the interplay of precession and damping determines the specific heat, magnetization, magnetocaloric effect, susceptibility and scattering rates. We point out that the precession can change the sign of the leading \sqrt{T} correction to the specific heat coefficient c(T)/T and can induce a characteristic maximum in c(T)/T for certain parameters. We argue that the susceptibility \chi =\partial M/\partial B is the thermodynamic quantity which shows the most significant change upon approaching the quantum critical point and which gives experimental access to the (dangerously irrelevant) spin-spin interactions.Comment: 12 pages, 8 figure

The population of white dwarf binaries with hot subdwarf companions

Hot subdwarfs (sdBs) are core helium-burning stars, which lost almost their entire hydrogen envelope in the red-giant phase. Since a high fraction of those stars are in close binary systems, common envelope ejection is an important formation channel. We identified a total population of 51 close sdB+WD binaries based on time-resolved spectroscopy and multi-band photometry, derive the WD mass distribution and constrain the future evolution of these systems. Most WDs in those binaries have masses significantly below the average mass of single WDs and a high fraction of them might therefore have helium cores. We found 12 systems that will merge in less than a Hubble time and evolve to become either massive C/O WDs, AM\,CVn systems, RCrB stars or even explode as supernovae type Ia.Comment: 5 pages, 2 figures, to appear in the proceedings of the 19th European White Dwarf Workshop, ASP Conf. Se

Green's function for a Schroedinger operator and some related summation formulas

Summation formulas are obtained for products of associated Lagurre polynomials by means of the Green's function K for the Hamiltonian H = -{d^2\over dx^2} + x^2 + Ax^{-2}, A > 0. K is constructed by an application of a Mercer type theorem that arises in connection with integral equations. The new approach introduced in this paper may be useful for the construction of wider classes of generating function.Comment: 14 page

Eisosome ultrastructure and evolution in fungi, microalgae, and lichens

Eisosomes are among the few remaining eukaryotic cellular differentations that lack a defined function(s). These trough-shaped invaginations of the plasma membrane have largely been studied in Saccharomyces cerevisiae, in which their associated proteins, including two BAR domain proteins, have been identified, and homologues have been found throughout the fungal radiation. Using quick-freeze deep-etch electron microscopy to generate high-resolution replicas of membrane fracture faces without the use of chemical fixation, we report that eisosomes are also present in a subset of red and green microalgae as well as in the cysts of the ciliate Euplotes. Eisosome assembly is closely correlated with both the presence and the nature of cell walls. Microalgal eisosomes vary extensively in topology and internal organization. Unlike fungi, their convex fracture faces can carry lineage-specific arrays of intramembranous particles, and their concave fracture faces usually display fine striations, also seen in fungi, that are pitched at lineage-specific angles and, in some cases, adopt a broad-banded patterning. The conserved genes that encode fungal eisosome-associated proteins are not found in sequenced algal genomes, but we identified genes encoding two algal lineage-specific families of predicted BAR domain proteins, called Green-BAR and Red-BAR, that are candidate eisosome organizers. We propose a model for eisosome formation wherein (i) positively charged recognition patches first establish contact with target membrane regions and (ii) a (partial) unwinding of the coiled-coil conformation of the BAR domains then allows interactions between the hydrophobic faces of their amphipathic helices and the lipid phase of the inner membrane leaflet, generating the striated patterns

Order and nFl Behavior in UCu4Pd

We have studied the role of disorder in the non-Fermi liquid system UCu4Pd using annealing as a control parameter. Measurement of the lattice parameter indicates that this procedure increases the crystallographic order by rearranging the Pd atoms from the 16e to the 4c sites. We find that the low temperature properties depend strongly on annealing. Whereas the non-Fermi liquid behavior in the specific heat can be observed over a larger temperature range after annealing, the clear non-Fermi liquid behavior of the resistivity of the unannealed sample below 10 K disappears. We come to the conclusion that this argues against the Kondo disorder model as an explanation for the non-Fermi liquid properties of both as-prepared and annealed UCu4Pd

Plasma membrane deformation by circular arrays of ESCRT-III protein filaments

Endosomal sorting complex required for transport III (ESCRT-III) proteins function in multivesicular body biogenesis and viral budding. They are recruited from the cytoplasm to the membrane, where they assemble into large complexes. We used “deep-etch” electron microscopy to examine polymers formed by the ESCRT-III proteins hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B). When overexpressed, these proteins target to endosomes and the plasma membrane. Both hSnf7 proteins assemble into regular approximately 5-nm filaments that curve and self-associate to create circular arrays. Binding to a coexpressed adenosine triphosphate hydrolysis–deficient mutant of VPS4B draws these filaments together into tight circular scaffolds that bend the membrane away from the cytoplasm to form buds and tubules protruding from the cell surface. Similar buds develop in the absence of mutant VPS4B when hSnf7-1 is expressed without its regulatory C-terminal domain. We demonstrate that hSnf7 proteins form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding. We suggest that ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies

Examine the species and beam-energy dependence of particle spectra using Tsallis Statistics

Tsallis Statistics was used to investigate the non-Boltzmann distribution of particle spectra and their dependence on particle species and beam energy in the relativistic heavy-ion collisions at SPS and RHIC. Produced particles are assumed to acquire radial flow and be of non-extensive statistics at freeze-out. J/psi and the particles containing strangeness were examined separately to study their radial flow and freeze-out. We found that the strange hadrons approach equilibrium quickly from peripheral to central A+A collisions and they tend to decouple earlier from the system than the light hadrons but with the same final radial flow. These results provide an alternative picture of freeze-outs: a thermalized system is produced at partonic phase; the hadronic scattering at later stage is not enough to maintain the system in equilibrium and does not increase the radial flow of the copiously produced light hadrons. The J/psi in Pb+Pb collisions at SPS is consistent with early decoupling and obtains little radial flow. The J/psi spectra at RHIC are also inconsistent with the bulk flow profile.Comment: 12 pages, 4 figures, added several references and some clarifications et

Specific heat of heavy fermion CePd2Si2 in high magnetic fields

We report specific heat measurements on the heavy fermion compound CePd2Si2 in magnetic fields up to 16 T and in the temperature range 1.4-16 K. A sharp peak in the specific heat signals the antiferromagnetic transition at T_N ~ 9.3 K in zero field. The transition is found to shift to lower temperatures when a magnetic field is applied along the crystallographic a-axis, while a field applied parallel to the tetragonal c-axis does not affect the transition. The magnetic contribution to the specific heat below T_N is well described by a sum of a linear electronic term and an antiferromagnetic spin wave contribution. Just below T_N, an additional positive curvature, especially at high fields, arises most probably due to thermal fluctuations. The field dependence of the coefficient of the low temperature linear term, gamma_0, extracted from the fits shows a maximum at about 6 T, at the point where an anomaly was detected in susceptibility measurements. The relative field dependence of both T_N and the magnetic entropy at T_N scales as [1-(B/B_0)^2] for B // a, suggesting the disappearance of antiferromagnetism at B_0 ~ 42 T. The expected suppression of the antiferromagnetic transition temperature to zero makes the existence of a magnetic quantum critical point possible.Comment: to be published in Journal of Physics: Condensed Matte