In Vivo Imaging of Transplanted Islets with ^(64)Cu-DO3A-VS-Cys^(40)-Exendin-4 by Targeting GLP-1 Receptor

Glucagon-like peptide 1 receptor (GLP-1R) is highly expressed in pancreatic islets, especially on β-cells. Therefore, a properly labeled ligand that binds to GLP-1R could be used for in vivo pancreatic islet imaging. Because native GLP-1 is degraded rapidly by dipeptidyl peptidase-IV (DPP-IV), a more stable agonist of GLP-1 such as Exendin-4 is a preferred imaging agent. In this study, DO3A-VS-Cys^(40)-Exendin-4 was prepared through the conjugation of DO3A-VS with Cys^(40)-Exendin-4. The in vitro binding affinity of DO3A-VS-Cys^(40)-Exendin-4 was evaluated in INS-1 cells, which overexpress GLP-1R. After ^(64)Cu labeling, biodistribution studies and microPET imaging of ^(64)Cu-DO3A-VS-Cys^(40)-Exendin-4 were performed on both subcutaneous INS-1 tumors and islet transplantation models. The subcutaneous INS-1 tumor was clearly visualized with microPET imaging after the injection of ^(64)Cu-DO3A-VS-Cys^(40)-Exendin-4. GLP-1R positive organs, such as pancreas and lung, showed high uptake. Tumor uptake was saturable, reduced dramatically by a 20-fold excess of unlabeled Exendin-4. In the intraportal islet transplantation models, ^(64)Cu-DO3A-VS-Cys^(40)-Exendin-4 demonstrated almost two times higher uptake compared with normal mice. ^(64)Cu-DO3A-VS-Cys^(40)-Exendin-4 demonstrated persistent and specific uptake in the mouse pancreas, the subcutaneous insulinoma mouse model, and the intraportal human islet transplantation mouse model. This novel PET probe may be suitable for in vivo pancreatic islets imaging in the human

Electron spin resonance of Er–oxygen complexes in GaAs grown by metal organic chemical vapor deposition

We have performed electron spin resonance (ESR) measurements on Er-doped GaAs grown with oxygen codoping by metal organic chemical vapor deposition. An isotropic line (an effective g value, g = 5.95) which had been already reported was observed in samples without oxygen codoping. On the other hand, for samples with oxygen codoping other strong anisotropic ESR lines originated from four kinds of Er3 + (4f11) centers (A, B, C, and D) were newly observed in addition to the weaker isotropic line. The anisotropic g tensors obtained by analyzing the angular dependence of the ESR lines indicate that B and C centers are of orthorhombic C2v symmetry, A center has lower symmetry than orthorhombic symmetry, and D center is of trigonal C3i symmetry. The ESR intensities of A, B, and C centers were approximately two orders of magnitude higher than that of the isotropic line with g = 5.95. The ESR intensity of D center was one order of magnitude lower than those of A, B, and C. The Er concentration dependence of the relative ESR intensities of these centers was investigated, which indicates (i) the ESR intensities of A and D increase with increasing Er concentration, and (ii) those of B and C are saturated above the Er concentration [Er] >= 1018 cm – 3. The ESR measurement under light illumination, as well as the Er concentration dependence, suggests that the B center with C2v symmetry corresponds to the dominant Er luminescent center under host photoexcitation

Demographic history and interspecific hybridization of four Shorea species (Dipterocarpaceae) from Peninsular Malaysia inferred from nucleotide polymorphism in nuclear gene regions

Shorea acuminata Dyer, Shorea curtisii Dyer ex King, Shorea leprosula Miq., and Shorea parvifolia Dyer are dominant tree species in the tropical rainforest of Peninsular Malaysia, which experienced several climatic changes during Pleistocene. To investigate the current population structure and demographic history of these species, we analyzed levels and patterns of nucleotide polymorphism of the nuclear gene region PgiC. We also used sequence data of the GapC gene region obtained in our previous study. Negative Tajima's D values observed in both investigated gene regions for S. curtisii, S. leprosula, and S. parvifolia implied that all three species have experienced population expansion events. Little to moderate levels of population differentiation in S. acuminata and S. curtisii suggested recent divergence of the investigated populations after postglacial colonization of the Peninsular Malaysia. In addition, some haplotypes were similar or identical to haplotypes of the other species. The existence of such haplotypes could be partially explained by interspecific hybridization. Indeed, we found some putative hybrid individuals. Interspecific hybridization among closely related species might have contributed to the polymorphism of the investigated species

Statistics of Substructures in Dark Matter Haloes

We study the amount and distribution of dark matter substructures within dark matter haloes, using a large set of high-resolution simulations ranging from group size to cluster size haloes, and carried our within a cosmological model consistent with WMAP 7-year data. In particular, we study how the measured properties of subhaloes vary as a function of the parent halo mass, the physical properties of the parent halo, and redshift. The fraction of halo mass in substructures increases with increasing mass. There is, however, a very large halo-to-halo scatter that can be explained only in part by a range of halo physical properties, e.g. concentration. At given halo mass, less concentrated haloes contain significantly larger fractions of mass in substructures because of the reduced strength of tidal disruption. Most of the substructure mass is located at the outskirts of the parent haloes, in relatively few massive subhaloes. This mass segregation appears to become stronger at increasing redshift, and should reflect into a more significant mass segregation of the galaxy population at different cosmic epochs. When haloes are accreted onto larger structures, their mass is significantly reduced by tidal stripping. Haloes that are more massive at the time of accretion (these should host more luminous galaxies) are brought closer to the centre on shorter time-scales by dynamical friction, and therefore suffer of a more significant stripping. The halo merger rate depends strongly on the environment with substructure in more massive haloes suffering more important mergers than their counterparts residing in less massive systems. This should translate into a different morphological mix for haloes of different mass.Comment: 13 pages, 11 figures and 1 table. MNRAS 2011 in pres

Band Calculation for Ce-compounds on the basis of Dynamical Mean Field Theory

The band calculation scheme for $f$ electron compounds is developed on the basis of the dynamical mean field theory (DMFT) and the LMTO method. The auxiliary impurity problem is solved by a method named as NCA$f^{2}$v', which includes the correct exchange process of the $f^{1} \to f^{2}$ virtual excitation as the vertex correction to the non-crossing approximation (NCA) for the $f^{1} \to f^{0}$ fluctuation. This method leads to the correct magnitude of the Kondo temperature, $T_{\rm K}$, and makes it possible to carry out quantitative DMFT calculation including the crystalline field (CF) and the spin-orbit (SO) splitting of the self-energy. The magnetic excitation spectra are also calculated to estimate $T_{\rm K}$. It is applied to Ce metal and CeSb at T=300 K as the first step. In Ce metal, the hybridization intensity (HI) just below the Fermi energy is reduced in the DMFT band. The photo-emission spectra (PES) have a conspicuous SO side peak, similar to that of experiments. $T_{\rm K}$ is estimated to be about 70 K in $\gamma$-Ce, while to be about 1700 K in $\alpha$-Ce. In CeSb, the double-peak-like structure of PES is reproduced. In addition, $T_{\rm K}$ which is not so low is obtained because HI is enhanced just at the Fermi energy in the DMFT band.Comment: 30pages, 18 figure

Vortex Lattice Structures of a Bose-Einstein Condensate in a Rotating Lattice Potential

We study vortex lattice structures of a trapped Bose-Einstein condensate in a rotating lattice potential by numerically solving the time-dependent Gross-Pitaevskii equation. By rotating the lattice potential, we observe the transition from the Abrikosov vortex lattice to the pinned lattice. We investigate the transition of the vortex lattice structure by changing conditions such as angular velocity, intensity, and lattice constant of the rotating lattice potential.Comment: 6 pages, 8 figures, submitted to Quantum Fluids and Solids Conference (QFS 2006

Engaging Undergraduates in Science Research: Not Just About Faculty Willingness.

Despite the many benefits of involving undergraduates in research and the growing number of undergraduate research programs, few scholars have investigated the factors that affect faculty members' decisions to involve undergraduates in their research projects. We investigated the individual factors and institutional contexts that predict faculty members' likelihood of engaging undergraduates in their research project(s). Using data from the Higher Education Research Institute's 2007-2008 Faculty Survey, we employ hierarchical generalized linear modeling to analyze data from 4,832 science, technology, engineering, and mathematics (STEM) faculty across 194 institutions to examine how organizational citizenship behavior theory and social exchange theory relate to mentoring students in research. Key findings show that faculty who work in the life sciences and those who receive government funding for their research are more likely to involve undergraduates in their research project(s). In addition, faculty at liberal arts or historically Black colleges are significantly more likely to involve undergraduate students in research. Implications for advancing undergraduate research opportunities are discussed