Evaluation of total and non-fatty ether extract in feeds and cattle feces using two analytical methods

AbstractThe objective of this study was to evaluate ether extract (EE) concentrations, pigments, and wax in forages (n=14), concentrates (n=23), and cattle feces (n=100) using extraction methods recommended by Association of Official Analytical Chemists (AOAC; method 920.39) and American Oil Chemist's Society (AOCS; method Am 5-04). The EE contents were compared by adjusting a linear regression model for each sample type. For the feces and forage samples, the EE contents produced by the AOCS method were greater (P<0.05) compared to those obtained using the AOAC method. No differences between methods were observed in EE content of concentrates (P>0.05). Concentration of vegetable pigments and wax were evaluated by using analysis for variance. Vegetable pigments were lower (P<0.05) in the post-extraction residues using the AOCS method, than the AOAC method, indicating greater participation of vegetable pigments in the EE. No differences were observed between the methods in wax concentration of the post-extraction residues (P>0.05). The quantification method of the EE content that is recommended by AOCS is not suggested for analyses of forage and feces of ruminants because it possibly increases the removal of non-fatty material, mostly pigments, in comparison to the method recommended by AOAC

Efficiency of lysine utilization by growing steers

Citation: Batista, E. D., Hussein, A. H., Detmann, E., Miesner, M. D., & Titgemeyer, E. C. (2016). Efficiency of lysine utilization by growing steers. Journal of Animal Science, 94(2), 648-655. doi:10.2527/jas2015-9716This study evaluated the efficiency of Lys utilization by growing steers. Five ruminally cannulated Holstein steers (165 +/- 8 kg) housed in metabolism crates were used in a 6 x 6 Latin square design; data from a sixth steer was excluded due to erratic feed intake. All steers were limit fed (2.46 kg DM/d), twice daily, diets low in RUP (81% soybean hulls, 8% wheat straw, 6% cane molasses, and 5% vitamins and minerals). Treatments were 0, 3, 6, 9, 12, and 15 g/d of L-Lys continuously abomasally infused. To prevent AA other than Lys from limiting performance, a mixture providing all essential AA to excess was continuously abomasally infused. Additional continuous infusions included 10 g urea/d, 200 g acetic acid/d, 200 g propionic acid/d, and 50 g butyric acid/d to the rumen and 300 g glucose/d to the abomasum. These infusions provided adequate ruminal ammonia and increased energy supply without increasing microbial protein supply. Each 6-d period included 2 d for adaptation and 4 d for total fecal and urinary collections for measuring N balance. Blood was collected on d 6 (10 h after feeding). Diet OM digestibility was not altered (P >= 0.66) by treatment and averaged 73.7%. Urinary N excretion was decreased from 32.3 to 24.3 g/d by increasing Lys supplementation to 9 g/d, with no further reduction when more than 9 g/d of Lys was supplied (linear and quadratic, P < 0.01). Changes in total urinary N excretion predominantly were due to changes in urinary urea N. Increasing Lys supply from 0 to 9 g/d increased N retention from 21.4 to 30.7 g/d, with no further increase beyond 9 g/d of Lys (linear and quadratic, P < 0.01). Break-point analysis estimated maximal N retention at 9 g/d supplemental Lys. Over the linear response surface of 0 to 9 g/d Lys, the efficiency of Lys utilization for protein deposition was 40%. Plasma urea N tended to be linearly decreased (P = 0.06) by Lys supplementation in agreement with the reduction in urinary urea N excretion. Plasma concentrations of Lys linearly increased (P < 0.001), but Leu, Ser, Val, and Tyr (P <= 0.02) were linearly reduced by Lys supplementation, likely reflecting increased uptake for protein deposition. In our model, Lys supplementation promoted significant increases in N retention and was maximized at 9 g/d supplemental Lys with an efficiency of utilization of 40%

Nutritional requirements of energy, protein and macrominerals for maintenance and weight gain of young crossbred Nellore × Holstein bulls on pasture.

The objective of this study was to estimate requirements of energy, protein and macrominerals of young Nellore/Holstein crossbreds bulls supplemented on pastures of Brachiaria decumbens Stapf. Thirty-five young bulls, at 8.53±0.18 months of age and with initial body weight of 230.6±6.1 kg were used. Ten animals were slaughtered as reference, in different weight range, and the other animals were slaughtered at the end of the experimental period. For estimate of net energy requirements for weight, a regression equation between log of retained energy (RE) and log of empty body weight gain (EBWG) was constructed. Net requirements of Ca, P, Mg, Na and K were determined by the equation Y' = a.b.Xb-1, in which a and b represent the intercept and the coefficient of equation of prediction of macrominerals in body content, respectively. Requirements of metabolizable energy for maintenance (MEm) were obtained from retained energy in function of metabolizable energy intake (MEI). The requirements of MEm of Nellore/Holstein crossbreds young bulls on pasture was 125 kcal/EBW0.75/day. The efficiency of ME utilization for maintenance (km) of grazing Nellore/Holstein crossbred young bulls was 0.58 and 0.24 for gain. The total metabolizable protein requirements for an animal with 400 kg and with average daily gain of 1.0 kg, were 638.36 g/day. The dietetic requirements of Ca and P for an animal with 400 kg BW were 0.49 and 0.21% of DM, respectively. Daily metabolizable energy requirement for maintenance of grazing Nellore/Holstein crossbred young bulls was 11.6% greater than the values found for cattle in feedlot in Brazil (112 kcal/kg EBW0.75)

Supplementation levels for growing beef cattle grazing in the dry-rainy transition season.

The objective of this experiment was to study the effects of different levels of supplementation on the performance, intake, digestibility, pH and rumen ammonia concentration in growing bulls grazing Brachiaria decumbens Stapf. pasture, during the dry-rainy transition season. For evaluation of performance, intake and digestibility, it was used 25 non-castrated steers at 11 months of age and initial average body weight of 270 kg, grouped in five plots of five animals each, following a completely randomized design. Each plot received one of the following feeding treatments: mineral mixture and supplement at the proportion of 0.5, 1.0, 1.5 and 2.0 kg/animal/day, corresponding to 0.18, 0.36, 0.54 and 0.72% of the average body weight of the animals. The area designated for the animals was constituted of five 2.0-ha paddocks. For the evaluation of the pH and rumen ammonia concentration, five crossbred non-castrated steers were used, with average body weight of 240 kg, fistulated in the esophagus, rumen and abomasum, disposed in a 5 × 5 Latin square, with five treatments and five experimental periods. Animal performance behaved in a positive linear manner according to the supplementation levels, responding with an increase of more than 80% on weight gains of the animals. Intakes of total and pasture dry matter (DM), organic matter and neutral detergent fiber were not influenced by supplementation. Intakes of crude protein, nonfibrous carbohydrates, ether extract and total digestible nutrients as well as nutrient digestibility and rumen ammonia concentration showed a positive linear pattern in response to supplementation levels. Increasing levels of concentrate supplementation influence positively the performance of bulls growing on pastures during the dry-rainy transition season

Effects of varying ruminally undegradable protein supplementation on forage digestion, nitrogen metabolism, and urea kinetics in Nellore cattle fed low-quality tropical forage

Citation: Batista, E. D., Detmann, E., Titgemeyer, E. C., Valadares, S. C., Valadares, R. F. D., Prates, L. L., . . . Paulino, M. F. (2016). Effects of varying ruminally undegradable protein supplementation on forage digestion, nitrogen metabolism, and urea kinetics in Nellore cattle fed low-quality tropical forage. Journal of Animal Science, 94(1), 201-216. doi:10.2527/jas2015-9493Effects of supplemental RDP and RUP on nutrient digestion, N metabolism, urea kinetics, and muscle protein degradation were evaluated in Nellore heifers (Bos indicus) consuming low-quality signal grass hay (5% CP and 80% NDF, DM basis). Five ruminally and abomasally cannulated Nellore heifers (248 +/- 9 kg) were used in a 5 x 5 Latin square. Treatments were the control (no supplement) and RDP supplementation to meet 100% of the RDP requirement plus RUP provision to supply 0, 50, 100, or 150% of the RUP requirement. Supplemental RDP (casein plus NPN) was ruminally dosed twice daily, and RUP supply (casein) was continuously infused abomasally. Jugular infusion of [(NN)-N-15-N-15]-urea with measurement of enrichment in urine was used to evaluate urea kinetics. The ratio of urinary 3-methylhistidine to creatinine was used to estimate skeletal muscle protein degradation. Forage NDF intake (2.48 kg/d) was not affected (P >= 0.37) by supplementation, but supplementation did increase ruminal NDF digestion (P < 0.01). Total N intake (by design) and N retention increased (P < 0.001) with supplementation and also linearly increased with RUP provision. Urea entry rate and gastrointestinal entry rate of urea were increased by supplementation (P < 0.001). Supplementation with RUP linearly increased (P = 0.02) urea entry rate and tended (P = 0.07) to linearly increase gastrointestinal entry rate of urea. Urea use for anabolic purposes tended (P = 0.07) to be increased by supplementation, and RUP provision also tended (P = 0.08) to linearly increase the amount of urea used for anabolism. The fraction of recycled urea N incorporated into microbial N was greater (P < 0.001) for control (22%) than for supplemented (9%) heifers. Urinary 3-methylhistidine: creatinine of control heifers was more than double that of supplemented heifers (P < 0.001). Control heifers reabsorbed a greater (P < 0.001) fraction of urea from the renal tubule than did supplemented heifers. Overall, unsupplemented heifers had greater mobilization of AA from myofibrillar protein, which provided N for urea synthesis and subsequent recycling. Supplemental RUP, when RDP was supplied, not only increased N retention but also supported increased urea N recycling and increased ruminal microbial protein synthesis

Ofertas de suplementos múltiplos para tourinhos Nelore na fase de recria em pastagens durante o período da seca: desempenho produtivo e características nutricionais.

Avaliaram-se o desempenho produtivo e as características nutricionais em tourinhos Nelore em fase de recria, não-castrados, recebendo diferentes ofertas de suplementos em pastagem de Brachiaria decumbens, de julho a setembro de 2006. A área foi dividida em cinco piquetes de 2,0 ha, com disponibilidade média de matéria seca (MS) e matéria seca potencialmente digestível (MSpd) de 3,88 e 2,22 t/ha, respectivamente. Foram utilizados 30 bezerros com peso e idade iniciais médios de 230,0 ± 6,14 kg e 8,5 ± 0,18 meses, em delineamento inteiramente casualizado contendo cinco tratamentos: quatro ofertas de suplementos em comparação a mistura mineral (MM). Foram avaliados a MM (60 g/animal) e suplementos múltiplos, fornecidos diariamente nas quantidades de 0,5; 1,0; 1,5 e 2,0 kg/animal para suprir 300 g/dia de PB. Os animais responderam ao uso de suplementos múltiplos, ganhando mais peso (88,72%) e apresentaram maiores níveis séricos de N-ureia (19,95 vs. 9,66) em comparação àqueles que receberam mistura mineral. Os animais sob suplementação apresentaram maiores consumos de MS, MO, EE, FDN, CNF e NDT e esses consumos aumentaram com os níveis de oferta de suplemento. A suplementação aumentou o coeficiente de digestão da maioria das variáveis avaliadas, independentemente do nível de suplemento fornecido. Os níveis mais moderados (0,5 kg) de suplementos múltiplos podem ser fornecidos aos tourinhos em fase de recria durante o período da seca para estimular o consumo de pasto, porém a máxima produção microbiana é obtida com oferta de 1,34 kg de suplemento. As melhores respostas de desempenho e características nutricionais podem ser obtidas com o fornecimento diário de suplemento em níveis de 0,5 a 1,34 kg/animal

On the estimation of non-fibrous carbohydrates in feeds and diets

Os carboidratos não fibrosos (CNF) contemplam a fração dos alimentos com elevada capacidade de produção de energia, destacando-se o amido, os açúcares, a pectina, etc. Por constituir a porção do alimento estimada pela diferença entre o todo a as partes avaliadas analiticamente (proteína bruta, matéria mineral, extrato etéreo e fibra em detergente neutro), o teor de CNF tende a absorver os erros cometidos na avaliação de cada parte. Aspectos da estimação do teor de CNF em alimentos e dietas são apresentados e discutidos