TP53-induced glycolysis and apoptosis regulator promotes proliferation and invasiveness of nasopharyngeal carcinoma cells

The TP53induced glycolysis and apoptosis regulator (TIGAR) is the protein product of the p53 target gene, C12orf5. TIGAR blocks glycolysis and promotes cellular metabolism via the pentose phosphate pathway; it promotes the production of cellular nicotinamide adenine dinucleotide phosphate (NADPH), which leads to enhanced scavenging of intracellular reactive oxygen species, and inhibition of oxidative stressinduced apoptosis in normal cells. Our previous study identified a novel nucleoside analog that inhibited cellular growth and induced apoptosis in nasopharyngeal carcinoma (NPC) cell lines via downregulation of TIGAR expression. Furthermore, the growth inhibitory effects of cMet tyrosine kinase inhibitors were ameliorated by the overexpression of TIGAR in the NPC cell lines. These results indicate a significant role for TIGAR expression in the survival of NPCs. The present study aimed to further define the function of TIGAR expression in NPC cells. In total, 36 formalinfixed, paraffinembedded NPC tissue samples were obtained for the immunohistochemical determination of TIGAR expression. The effects of TIGAR expression on cell proliferation, NADPH production and cellular invasiveness were also assessed in NPC cell lines. Overall, TIGAR was overexpressed in 27/36 (75%) of the NPC tissues compared with the adjacent noncancer epithelial cells. Similarly, TIGAR overexpression was also observed in a panel of six NPC cell lines compared with normal NP460 hTert and Het1A cell lines. TIGAR overexpression led to increased cellular growth, NADPH production and invasiveness of the NPC cell lines, whereas a knockdown of TIGAR expression resulted in significant inhibition of cellular growth and invasiveness. The expression of the two mesenchymal markers, fibronectin and vimentin, was increased by TIGAR overexpression, but reduced following TIGARknockdown. The present study revealed that TIGAR overexpression led to increased cellular growth, NADPH production and invasiveness, and the maintenance of a mesenchymal phenotype, in NPC tissues.published_or_final_versio

Boundary layer profile of decaying and non-decaying tropical storms near landfall

The vertical profile of the wind structure of translating tropical cyclones, including the associated azimuthal asymmetry, has been the subject of existing theoretical and observational studies using dropsondes. Most of these studies are based on data collected from relatively strong cyclones over the Atlantic. Here we explore the tropical cyclone boundary layer wind profile of mainly relatively weak landfalling cyclones near Hong Kong. We find that decaying tropical storms have a much larger mid- to low-level inflow angle than those that are intensifying or in steady-state. The inflow angles of intensifying, steady-state and decaying tropical storms converge towards the top of the boundary layer. The wind speed reduces through the boundary layer in a similar way in all three cases. The combination of these factors means that decaying tropical storms have stronger inflow than intensifying and steady-state ones. We attribute these local effects to remote enhanced surface friction over land when the storms are weakening

Changes in the upper airway, hyoid bone and craniofacial morphology between patients treated with headgear activator and Herbst appliance: A retrospective study on lateral cephalometry

Background: The present study compared the treatment changes in the upper airway, hyoid bone position and craniofacial morphology between two groups of children with skeletal class II malocclusion treated with the headgear activator (HGA) and Herbst appliance (Herbst). Setting and sample population: Orthodontic population from the Faculty of Dentistry of the University of Hong Kong. Methods: Thirty-four skeletal class II patients treated with the HGA (17 patients, mean age 10.6 ± 1.5 years) and the Herbst (17 patients, mean age 11.0 ± 1.4 years) were matched for sex, age, overjet, skeletal class and mandibular divergence. The patients received lateral cephalometric radiographs (LCRs) at the beginning of treatment (T1 ), after treatment (T2 ) and at follow-up (T3 ). In the HGA group, patients underwent LCRs 7 months before the beginning of treatment (T0 ), which were used as growth reference for intra-group comparison. Paired Student's t tests were used for intra- and inter-group comparisons (α = .05). Results: Treatment changes (T2 -T1 ) did not differ significantly between the groups. However, at follow-up (T3 -T1 ) the Herbst group showed a smaller increase than the HGA group in the vertical position of the hyoid bone relative to the Frankfort plane (P = .013) and mandibular plane (P = .013). Conclusions: There were no significant differences in the upper airway, hyoid bone position and craniofacial morphology between the groups at the end of treatment. However, the Herbst may provide better long-term control of the vertical position of the hyoid bone than the HGA in children with skeletal class II malocclusion

Anti-Inflammatory and Wound Healing Effect of Acupuncture in Treating Phonotraumatic Vocal Fold Pathologies

Speech-Language Pathology Session 2BConference Theme: Care of the Professional VoiceBackground: Acupuncture has been shown to be effective in bringing about improvements in benign vocal lesions and vocal function (Yiu et al., 2006). The underlying biological mechanism of acupuncture in the treatment of benign vocal pathologies is not fully understood yet. The improvement is, however, not a result of stress reduction that is often attributed to acupuncture (Kwong & Yiu, 2010). Objective: This study set out to investigate whether acupuncture would influence the anti-inflammatory process in vocal fold lesion healing ...published_or_final_versio

Using blood transcriptome analysis for Alzheimer's disease diagnosis and patient stratification

INTRODUCTION: Blood protein biomarkers demonstrate potential for Alzheimer's disease (AD) diagnosis. Limited studies examine the molecular changes in AD blood cells. METHODS: Bulk RNA-sequencing of blood cells was performed on AD patients of Chinese descent (n = 214 and 26 in the discovery and validation cohorts, respectively) with normal controls (n = 208 and 38 in the discovery and validation cohorts, respectively). Weighted gene co-expression network analysis (WGCNA) and deconvolution analysis identified AD-associated gene modules and blood cell types. Regression and unsupervised clustering analysis identified AD-associated genes, gene modules, cell types, and established AD classification models. RESULTS: WGCNA on differentially expressed genes revealed 15 gene modules, with 6 accurately classifying AD (areas under the receiver operating characteristics curve [auROCs] > 0.90). These modules stratified AD patients into subgroups with distinct disease states. Cell-type deconvolution analysis identified specific blood cell types potentially associated with AD pathogenesis. DISCUSSION: This study highlights the potential of blood transcriptome for AD diagnosis, patient stratification, and mechanistic studies. Highlights: We comprehensively analyze the blood transcriptomes of a well-characterized Alzheimer's disease cohort to identify genes, gene modules, pathways, and specific blood cells associated with the disease. Blood transcriptome analysis accurately classifies and stratifies patients with Alzheimer's disease, with some gene modules achieving classification accuracy comparable to that of the plasma ATN biomarkers. Immune-associated pathways and immune cells, such as neutrophils, have potential roles in the pathogenesis and progression of Alzheimer's disease

Integration of chromosomal microarray into paediatric clinical care in Hong Kong

Poster Presentation 9BACKGROUND AND AIMS: Chromosomal microarray (CMA) has emerged as a major tool to identify unbalanced chromosomal aberrations in children and is recommended as the first tiered investigation for intellectual disability, autism spectrum disorders and multiple congenital anomalies. While the clinical interpretation and genetic counseling remain as ongoing challenge, data about potential downstream benefits and harms of CMA is lacking, especially in paediatric ...postprin

Impact of Bacillus Calmette-Guérin (BCG) vaccination on postoperative mortality in patients with perioperative SARS-CoV-2 infection

There is little evidence around the potentially protective role of previous Bacillus Calmette-Guerin (BCG) vaccination on postoperative mortality in patients with perioperative SARS-CoV-2 vaccination. Prior BCG vaccination did not protect SARS-CoV-2 infected patients against postoperative pulmonary complications and 30-day mortality

Searching the academy: a Chinese Opera and the rule of Law

Session 8.13: Legal Aesthetics, Aesthetic’s Archives: Panelist 2This paper contributes to the emergence of chinese-language material in law and literature scholarship by exploring the legal issues in a canonical cantonese opera, Searching the Academy. At the center of the opera is the confrontation between a Confucian scholar-official, and a military officer in imperial china over who has the right of possession over a young bondswoman and hence, the right to decide whether she has committed an offence in law. The legal narrative deals with a fundamental 'rule of law' issue: the foundation of a system of rules from which it derives legitimate force that, in turn, has impact upon its everyday administration and enforcement. To elaborate the opera as historical discourse on the rule of law in a Chinese context, I will discuss the opera's beginnings in imperial China, its canonization in the early years of the People's Republic, and recent restaging in post-1997 Hong Kong

Searching the Academy (Soushuyuan 搜書院):: A Chinese Opera as Rule of Law and Legal Narrative

This paper explores the significance of legal narratives in a leading Chinese Opera in its contemporary contex