A comparison of methodologies for the staining and quantification of intracellular components of Arbuscular Mychorrizal (AM) fungi in the root cortex of two varieties of winter wheat

© 2019 The Authors. The definitive peer reviewed, edited version of this article is published in Access Microbiology, https://doi.org/10.1099/acmi.0.000083. This is an open-access article distributed under the terms of the Creative Commons Attribution License.Arbuscular Mychorrizal (AM) fungi are one of the most common fungal organisms to exist in symbiosis with terrestrial plants facilitating the growth and maintenance of arable crops. Wheat has been studied extensively for AM fungal symbiosis using the carcinogen trypan blue as the identifying stain for fungal components, namely arbuscles, vesicles and hyphal structures. The present study uses Sheaffer® blue ink with a lower risk as an alternative to this carcinogenic stain. Justification for this is determined by stained wheat root sections (n = 120), with statistically significant increases in the observed abundance of intracellular root cortical fungal structures stained with Sheaffer® blue ink compared to trypan blue for both Zulu (P = 0.003) and Siskin (P = 0.0003) varieties of winter wheat. This new alternative combines an improved quantification of intracellular fungal components with a lower hazard risk at a lower cost.Peer reviewe

P-mode observations on Alpha Cen A

We have made a clear detection of p-mode oscillations in the nearest solar-like G2V star $\alpha$ Cen A with the CORALIE spectrograph on the 1.2-m Swiss telescope at the ESO La Silla Observatory. We report the 5 nights of observation on this star in May 2001 during which 1260 high precision radial velocity measurements were obtained. The power spectrum clearly shows several identifiable peaks between 1.7 and 3 mHz. The average large splitting of 105.7 $\mu$Hz and the amplitude of about 35 cm s$^{-1}$ of these modes are in agreement with theoretical expectations.Comment: 4 pages, 4 figures, A&A Letter accepte

Domain walls in (Ga,Mn)As diluted magnetic semiconductor

We report experimental and theoretical studies of magnetic domain walls in an in-plane magnetized (Ga,Mn)As dilute moment ferromagnetic semiconductor. Our high-resolution electron holography technique provides direct images of domain wall magnetization profiles. The experiments are interpreted based on microscopic calculations of the micromagnetic parameters and Landau-Lifshitz-Gilbert simulations. We find that the competition of uniaxial and biaxial magnetocrystalline anisotropies in the film is directly reflected in orientation dependent wall widths, ranging from approximately 40 nm to 120 nm. The domain walls are of the N\'eel type and evolve from near-$90^{\circ}$ walls at low-temperatures to large angle [1$\bar{1}$0]-oriented walls and small angle [110]-oriented walls at higher temperatures.Comment: 5 pages, 4 figure

Molecular elasticity and the geometric phase

We present a method for solving the Worm Like Chain (WLC) model for twisting semiflexible polymers to any desired accuracy. We show that the WLC free energy is a periodic function of the applied twist with period 4 pi. We develop an analogy between WLC elasticity and the geometric phase of a spin half system. These analogies are used to predict elastic properties of twist-storing polymers. We graphically display the elastic response of a single molecule to an applied torque. This study is relevant to mechanical properties of biopolymers like DNA.Comment: five pages, one figure, revtex, revised in the light of referee's comments, to appear in PR

Measurement of viscous sound absorption at 50-150 kHz in a model turbid environment

The visco-thermal absorption of sound by suspended particulate matter can be reliably measured using a reverberation technique. This absorption may have an adverse effect on the performance of sonars operating at 50–300 kHz in coastal waters where suspensions are often present in significant concentrations. A series of experiments has been performed to study the viscous absorption by suspensions in the frequency range of 50–150 kHz. In the test volumes employed, the effect is small. It is therefore measured by taking the difference in reverberation times of a volume of water with and without particles. This greatly reduces the effect on the measurement of the other sources of absorption. Even so, it is necessary to design the experiment to characterize and minimize acoustic losses which occur at the surfaces of the container, the hydrophones, and their cables, and losses associated with bubbles and turbulence. These effects are discussed and results for particulate absorption for suspensions of spherical glass beads are presented and compared to theoretical predictions. Measured absorption agrees well with that predicted by theory for concentrations above 0.5 kg/m3 and up to 2.0 kg/m

The Skyrme energy functional and low lying 2+ states in Sn, Cd and Te isotopes

We study the predictive power of Skyrme forces with respect to low lying quadrupole spectra along the chains of Sn, Cd, and Te isotopes. Excitation energies and B(E2) values for the lowest quadrupole states are computed from a collective Schroedinger equation which as deduced through collective path generated by constraint Skyrme-Hartree-Fock (SHF) plus self-consistent cranking for the dynamical response. We compare the results from four different Skyrme forces, all treated with two different pairing forces (volume versus density-dependent pairing). The region around the neutron shell closure N=82 is very sensitive to changes in the Skyrme while the mid-shell isotopes in the region N<82 depend mainly on the adjustment of pairing. The neutron rich isotopes are most sensitive and depend on both aspects

Shell model study of the isobaric chains A=50, A=51 and A=52

Shell model calculations in the full pf-shell are carried out for the A=50, 51 and 52 isobars. The most frequently used effective interactions for the pf-shell, KB3 and FPD6 are revisited and their behaviour at the N=28 and Z=28 closures examined. Cures to their -relatively minor- defaults are proposed, and a new mass dependent version called KB3G is released. Energy spectra, electromagnetic transitions and moments as well as beta decay properties are computed and compared with the experiment and with the results of the earlier interactions. A high quality description is achieved. Other miscellaneous topics are addressed; the Coulomb energy differences of the yrast states of the mirror pair 51Mn-51Fe and the systematics of the magnetic moments of the N=28 isotones.Comment: 45 pages, 34 figures, Latex. Submitted for publicatio

Collective Modes of Tri-Nuclear Molecules

A geometrical model for tri-nuclear molecules is presented. An analytical solution is obtained provided the nuclei, which are taken to be prolately deformed, are connected in line to each other. Furthermore, the tri-nuclear molecule is composed of two heavy and one light cluster, the later sandwiched between the two heavy clusters. A basis is constructed in which Hamiltonians of more general configurations can be diagonalized. In the calculation of the interaction between the clusters higher multipole deformations are taken into account, including the hexadecupole one. A repulsive nuclear core is introduced in the potential in order to insure a quasi-stable configuration of the system. The model is applied to three nuclear molecules, namely $^{96}$Sr + $^{10}$Be + $^{146}$Ba, $^{108}$Mo + $^{10}$Be + $^{134}$Te and $^{112}$Ru + $^{10}$Be + $^{130}$Sn.Comment: 24 pages, 9 figure

Characteristics of solar-like oscillations of clusters simulated by stellar population synthesis

Using a stellar population-synthesis method, we studied the distributions of {\nu}_max and {\Delta}{\nu} of simulated clusters with various ages and metallicities. Except for the confirmed peak (RC peak) of {\Delta}{\nu} of red-clump (RC) stars, i.e. core-helium burning stars, there are a gap and a main sequence (MS) peak in the distributions of {\nu}_max and {\Delta}{\nu} of young clusters. The gap corresponds mainly to the Hertzsprung gap phase of evolution. The RC peak is caused by the fact that the radius of many RC stars near the zero-age horizontal branch concentrates in a certain range. The MS peak also results from the fact that many MS stars which are located in a certain mass range have an approximate radius in the early phase of MS. The MS peak barely exists in the simulated clusters with age < 1.0 Gyr. The location of the MS peak moves to a lower frequency with increasing age or metallicity, which may be applied to constrain the age and metallicity of young clusters. For the simulated clusters with Z = 0.02, the frequency of the location of the dominant RC peak increases with age when age < 1.2 Gyr, and then decreases with age when age > 1.2 Gyr; but it scarcely varies when age > 2.4 Gyr. This is relative to the degeneracy of the hydrogen-exhausted core at the time of helium ignition. In addition, the RC peak is not sensitive to the metallicity, especially for the clusters with age > 2.4 Gyr. Asteroseismical observation for clusters with age < 2.4 Gyr may aid in testing the theory of the degeneracy of the hydrogen-exhausted core. Moreover, for the clusters with 1.1 M{\odot} < M_hook < 1.3 M{\odot}, there are a MS gap and a peak on the left of the MS gap in the distributions of{\nu}_max and {\Delta}{\nu}, which may be applied to constrain the central hydrogen abundance of stars in the MS gap and the peak.Comment: Accepted for publication in MNRAS. 8 pages, 8 figure

Observing solar-like oscillations

We review techniques for measuring stellar oscillations in solar-type stars. Despite great efforts, no unambiguous detections have been made. A new method, based on monitoring the equivalent widths of strong lines, shows promise but is yet to be confirmed. We also discuss several subtleties, such as the need to correct for CCD non-linearities and the importance of data weighting