Detection of extended spectrum B-lactamases in urinary isolates of Klebsiella pneumoniae in relation to Bla SHV, Bla TEM and Bla CTX-M gene carriage

Background: Resistance to contemporary broad-spectrum β-lactam antibiotics mediated by extended-spectrum β-lactamases (ESBLs) is increasing worldwide. Klebsiella pneumoniae, an important cause of nosocomial and community acquired urinary tract infections has rapidly become the most common ESBL producing organism. We examined ESBL production in urinary isolates of K. pneumoniae in relation to the presence of bla SHV, bla TEM and bla CTX-M genes. Methods: Antibiotic resistance of 51 clinical isolates of K. pneumoniae was determined to amoxicillin, amikacin, ceftazidime, cefotaxime, cefteriaxon, ceftizoxime, gentamicin, ciprofloxacin and nitrofurantoin by disc diffusion. Minimum inhibitory concentrations were also measured for ceftazidime, cefotaxime, cefteriaxon, ceftizoxime and ciprofloxacin. ESBL production was detected by the double disc synergy test and finally, presence of the bla SHV, bla TEM and bla CTX-M genes were shown using specific primers and PCR. Results: Disc diffusion results showed that 96.08 % of the isolates were resistant to amoxicillin followed by 78.43 % resistance to nitrofurantoin, 49.02 % to amikacin and ceftazidime, 41.17 % to ceftriaxone, 37.25% resistance to cefotaxime and ceftizoxime, and 29.42 % to gentamicin and ciprofloxacin. Both resistant and intermediately resistant organisms were resistant in MIC determinations. Twenty two isolates (43.14%) carried bla SHV, 18 (35.29%) had bla TEM and 16 (31.37%) harbored bla CTX-M genes. ESBL production was present in 14 isolates (27.45 %) of which, 3 did not harbor any of the 3 genes. Among the non- ESBL producers, 9 lacked all 3 genes and 2 carried them all. Conclusion: No relation was found between gene presence and ESBL expression

Band-edge Bilayer Plasmonic Nanostructure for Surface Enhanced Raman Spectroscopy

Spectroscopic analysis of large biomolecules is critical in a number of applications, including medical diagnostics and label-free biosensing. Recently, it has been shown that Raman spectroscopy of proteins can be used to diagnose some diseases, including a few types of cancer. These experiments have however been performed using traditional Raman spectroscopy and the development of the Surface enhanced Raman spectroscopy (SERS) assays suitable for large biomolecules could lead to a substantial decrease in the amount of specimen necessary for these experiments. We present a new method to achieve high local field enhancement in surface enhanced Raman spectroscopy through the simultaneous adjustment of the lattice plasmons and localized surface plasmon polaritons, in a periodic bilayer nanoantenna array resulting in a high enhancement factor over the sensing area, with relatively high uniformity. The proposed plasmonic nanostructure is comprised of two interacting nanoantenna layers, providing a sharp band-edge lattice plasmon mode and a wide-band localized surface plasmon for the separate enhancement of the pump and emitted Raman signals. We demonstrate the application of the proposed nanostructure for the spectral analysis of large biomolecules by binding a protein (streptavidin) selectively on the hot-spots between the two stacked layers, using a low concentration solution (100 nM) and we successfully acquire its SERS spectrum

A unified approach to mode splitting and scattering loss in high-Q whispering-gallery-mode microresonators

Current theoretical treatment of mode splitting and scattering loss resulting from sub-wavelength scatterers attached to the surface of high-quality-factor whispering-gallery-mode microresonators is not satisfactory. Different models have been proposed for two distinct scatterer regimes, i.e., a-few- and many-scatterers. In addition, many experimental results seem difficult to understand within the existing theoretical framework. Here we develop a unified approach that applies to an arbitrary number of scatterers, which reveals the applicable conditions and the limits of the existing theoretical models. Moreover, many new understandings on mode splitting and scattering loss have been achieved, which are supported by numerical and experimental evidences. Such a unified approach is essential for the fundamental studies as well as the practical applications of mode splitting and scattering loss in high-quality-factor whispering-gallery-mode microresonators.Comment: 19 pages, 10 figure

Self-synchronization of Kerr-nonlinear Optical Parametric Oscillators

We introduce a new, reduced nonlinear oscillator model governing the spontaneous creation of sharp pulses in a damped, driven, cubic nonlinear Schroedinger equation. The reduced model embodies the fundamental connection between mode synchronization and spatiotemporal pulse formation. We identify attracting solutions corresponding to stable cavity solitons and Turing patterns. Viewed in the optical context, our results explain the recently reported $\pi$ and $\pi/2$ steps in the phase spectrum of microresonator-based optical frequency combs

Genome-wide gene expression profiling of stress response in a spinal cord clip compression injury model.

BackgroundThe aneurysm clip impact-compression model of spinal cord injury (SCI) is a standard injury model in animals that closely mimics the primary mechanism of most human injuries: acute impact and persisting compression. Its histo-pathological and behavioural outcomes are extensively similar to human SCI. To understand the distinct molecular events underlying this injury model we analyzed global mRNA abundance changes during the acute, subacute and chronic stages of a moderate to severe injury to the rat spinal cord.ResultsTime-series expression analyses resulted in clustering of the majority of deregulated transcripts into eight statistically significant expression profiles. Systematic application of Gene Ontology (GO) enrichment pathway analysis allowed inference of biological processes participating in SCI pathology. Temporal analysis identified events specific to and common between acute, subacute and chronic time-points. Processes common to all phases of injury include blood coagulation, cellular extravasation, leukocyte cell-cell adhesion, the integrin-mediated signaling pathway, cytokine production and secretion, neutrophil chemotaxis, phagocytosis, response to hypoxia and reactive oxygen species, angiogenesis, apoptosis, inflammatory processes and ossification. Importantly, various elements of adaptive and induced innate immune responses span, not only the acute and subacute phases, but also persist throughout the chronic phase of SCI. Induced innate responses, such as Toll-like receptor signaling, are more active during the acute phase but persist throughout the chronic phase. However, adaptive immune response processes such as B and T cell activation, proliferation, and migration, T cell differentiation, B and T cell receptor-mediated signaling, and B cell- and immunoglobulin-mediated immune response become more significant during the chronic phase.ConclusionsThis analysis showed that, surprisingly, the diverse series of molecular events that occur in the acute and subacute stages persist into the chronic stage of SCI. The strong agreement between our results and previous findings suggest that our analytical approach will be useful in revealing other biological processes and genes contributing to SCI pathology

The effect of luteal phase gonadotropin-releasing hormone antagonist administration on IVF outcomes in women at risk of OHSS

Gonadotropin-releasing hormone (GnRH) plays essential roles in embryo implantation, invasion of trophoblastic tissue, and steroid synthesis in the placenta. Objective: The aim of this study was to evaluate the effect of GnRH antagonist administration on pregnancy outcomes in early implantation period. Materials and Methods: In this retrospective study, 94 infertile women undergoing GnRH antagonist protocol who were at risk of ovarian hyperstimulation syndrome (OHSS) were included. Sixty-seven patients (group I) received Cetrorelix 0.25 mg/daily in the luteal phase for 3 days while in 27 participants (group II), it was not administered. Pregnancy outcomes were assessed based on chemical and clinical pregnancy rates. Results: The pregnancy outcomes were not significantly different between two groups (p=0.224). Conclusion: The present study proposed that luteal phase GnRH antagonist administration does not influence the chance of successful pregnancy outcomes

Towards Next Generation Neural Interfaces: Optimizing Power, Bandwidth and Data Quality

In this paper, we review the state-of-the-art in neural interface recording architectures. Through this we identify schemes which show the trade-off between data information quality (lossiness), computation (i.e. power and area requirements) and the number of channels. These trade-offs are then extended by considering the front-end amplifier bandwidth to also be a variable. We therefore explore the possibility of band-limiting the spectral content of recorded neural signals (to save power) and investigate the effect this has on subsequent processing (spike detection accuracy). We identify the spike detection method most robust to such signals, optimize the threshold levels and modify this to exploit such a strategy.Accepted versio

Capturing personality from Facebook photos and photo-related activities: How much exposure do you need?

Photo-related activities are noticeably prevalent among social media users. On Facebook, users predominantly communicate visually and manage their self-presentation. Such online behaviours tend to mimic what would be expected of individuals’ offline personalities. This study sought to address the link between Facebook users’ photo-related activities and the Big Five personality traits by encoding basic Facebook visual features. Content analysis on the actual profiles (n = 115) and multiple regression analyses revealed many associations as a manifestation of users’ characteristics. For instance, Neuroticism and Extraversion predicted more photo uploads. Conscientiousness was predictive of more self-generated albums and video uploads and Agreeableness predicted the average number of received ‘likes’ and ‘comments’ on profile pictures. Additionally, the Facebook experience in interaction with the personality factors was found to be influential on the type of photo-related activity and the level of photo participation of users. The findings provide evidence that Facebook users with various personality traits set up albums and upload photos differently. Given the uses and gratification model, users adapt the construction of their profiles and manage their interactions to gratify their psychological needs on Facebook

Self-synchronization Phenomena in the Lugiato-Lefever Equation

The damped driven nonlinear Schr\"odinger equation (NLSE) has been used to understand a range of physical phenomena in diverse systems. Studying this equation in the context of optical hyper-parametric oscillators in anomalous-dispersion dissipative cavities, where NLSE is usually referred to as the Lugiato-Lefever equation (LLE), we are led to a new, reduced nonlinear oscillator model which uncovers the essence of the spontaneous creation of sharply peaked pulses in optical resonators. We identify attracting solutions for this model which correspond to stable cavity solitons and Turing patterns, and study their degree of stability. The reduced model embodies the fundamental connection between mode synchronization and spatiotemporal pattern formation, and represents a novel class of self-synchronization processes in which coupling between nonlinear oscillators is governed by energy and momentum conservation.Comment: This manuscript is published in Physical Review A. Copyright 2017 by the American Physical Society. arXiv admin note: text overlap with arXiv:1602.0852