The accordian and the deep bowl of spaghetti: Eight researchers' experiences of using IPA as a methodology

Since 1996 Interpretative Phenomenological Analysis (IPA) has grown rapidly and been applied in areas outside its initial “home” of health psychology. However, explorations of its application from a researcher's perspective are scarce. This paper provides reflections on the experiences of eight individual researchers using IPA in diverse disciplinary fields and cultures. The research studies were conducted in the USA, Malaysia, Australia, New Zealand, Ireland and the UK by researchers with backgrounds in business management, consumer behaviour, mental health nursing, nurse education, applied linguistics, clinical psychology, health and education. They variously explored media awareness, employee commitment, disengagement from mental health services, in-vitro fertilisation treatment, student nurses' experience of child protection, second language acquisition in a university context, the male experience of spinal cord injury and academics experience of working in higher education and women’s experiences of body size and health practices. By bringing together intercultural, interdisciplinary experiences of using IPA, the paper discusses perceived strengths and weaknesses of IPA

Doing it the best way that we can : men's and women's experiences during the early stages of IVF: an interpretative phenomenological analysis

This research examined how men and women experience stress and coping during the early stages of IVF, focusing on time, gender and couples. Both members of three heterosexual couples took part separately in two or three semi-structured interviews over a six-month period, producing fourteen accounts. Interpretative Phenomenological Analysis was used to preserve participants' unique experiences alongside interpretation and generation of broader themes by the researcher. Infertility and fertility treatment were not always seen as stressful, but often as a problem to be tackled in the best way, both emotionally and practically. Stress arose from specific, time-limited issues. Participants' emotional responses were shaped by perceptions of the effect of stress on fertility, a desire to stay positive, and downward comparison with other fertility patients who were perceived to be coping poorly. Participants emphasised their choices as logical, careful decisions, weighing up multiple factors including alternatives like adoption, and temporal and financial investments. Over time, perceptions of IVF changed from a precise, technical process to one subject to luck and chance, although the process itself was perceived as becoming easier with experience. The study was originally positioned within the transactional stress and coping model, but a self-regulatory perspective provided a better fit for the data. The findings are linked to each model where appropriate, and the implications suggest use of the transactional model to understanding specific, time limited events, and a self-regulatory framework to explore general fertility treatment experiences. Suggestions for future work include greater use of the self-regulatory framework to study infertility and fertility treatment; paying attention to couples' willingness to adopt in shaping infertility experiences; conducting interviews at different times during treatment cycles, and during different treatment cycles; and using alternative data gathering methods including Internet Mediated Research

The Accordion and the Deep Bowl of Spaghetti: Eight Researchers\u27 Experiences of Using IPA as a Methodology

Since 1996 Interpretative Phenomenological Analysis (IPA) has grown rapidly and been applied in areas outside its initial “home” of health psychology. However, explorations of its application from a researcher\u27s perspective are scarce. This paper provides reflections on the experiences of eight individual researchers using IPA in diverse disciplinary fields and cultures. The research studies were conducted in the USA, Malaysia, Australia, New Zealand, Ireland and the UK by researchers with backgrounds in business management, consumer behaviour, mental health nursing, nurse education, applied linguistics, clinical psychology, health and education. They variously explored media awareness, employee commitment, disengagement from mental health services, in-vitro fertilisation treatment, student nurses\u27 experience of child protection, second language acquisition in a university context, the male experience of spinal cord injury and academics experience of working in higher education and women’s experiences of body size and health practices. By bringing together intercultural, interdisciplinary experiences of using IPA, the paper discusses perceived strengths and weaknesses of IPA

An interpretative phenomenological analysis of men’s and women’s coping strategy selection during early IVF treatment

Objectives: To describe the coping strategies that men and women adopted during the early stages of in vitro fertilisation treatment, and explore why and how they selected those strategies. Background: Previous research has identified coping strategies used during fertility treatment and the impact of those strategies on adjustment, but not how and why individuals choose the strategies they did, which is important for understanding coping strategy use as a self-regulatory process. Methods: Three heterosexual couples took part in two or three individual semi-structured interviews over six months, producing fourteen accounts, which were analysed using interpretative phenomenological analysis. Results: The emergent themes were: not dwelling on emotional issues; getting on with treatment; and keeping busy with other things. Participants selected coping strategies in a conscious, deliberate way, by making comparisons with other patients and by drawing on their broader, customary ways of coping. The strategies participants adopted made sense in the context of their long-term goals as well as their short-term treatment objectives. Conclusion: This research shows that for these participants, shorter-term behavioural strategies were informed by longer-term goals, which is consistent with a self-regulatory approach to understanding how people cope with the stress of treatment for infertility.N/

Exploring female academics' resiliency during the pandemic

Academics in Higher Education Institutions (HEIs) faced difficulties during the pandemic when moving from face-to-face instruction to online learning and teaching platforms. In particular, female academics were challenged to find unique and innovative methods of merging their professional responsibilities with personal commitments amid COVID-19 restrictions. In response to Harris’s (2022) endorsement of the fact that resiliency could be part of the solution in future-proofing education systems, three female academics from different geographic locations question how they maintained their personal and professional resiliency, and how their resiliency compared before and after the pandemic. This collaborative autoethnographical study was based on an epistemological interpretivist paradigm and used in vivo and descriptive coding to place the researchers’ narratives into themes and sub-themes including personal issues of blurred work boundaries, isolation from friends and family, concern for others’ health and the sacrifice of family time. Professional themes included ‘compassion fatigue’ when meeting student needs, demands as ‘mothering’ academics, and a desire for socialisation. It was found that resiliency was maintained both personally and professionally, but as a process rather than being measured in binary terms. Interestingly, it was strengthened when participants cultivated optimistic attitudes whilst taking the opportunity to ‘nest’ and practice ‘self care’ leading each participant to be more resilient after the pandemic, than before. Strategies are suggested for future proofing female academics’ resiliency as they transition to the new post-pandemic normal. Whilst research is available about how to maintain personal self care and professional development, this study is unique as it identifies the benefits for professional self care. Also, as an autoethnographic study the findings speak for female academics whose voices are normally heard as researchers, but are not researched themselves

British Gynaecological Cancer Society Recommendations for Evidence Based, Population Data Derived Quality Performance Indicators for Ovarian Cancer

Ovarian cancer survival in the UK lags behind comparable countries. Results from the ongoing National Ovarian Cancer Audit feasibility pilot (OCAFP) show that approximately 1 in 4 women with advanced ovarian cancer (Stage 2, 3, 4 and unstaged cancer) do not receive any anticancer treatment and only 51% in England receive international standard of care treatment, i.e., the combination of surgery and chemotherapy. The audit has also demonstrated wide variation in the percentage of women receiving anticancer treatment for advanced ovarian cancer, be it surgery or chemotherapy across the 19 geographical regions for organisation of cancer delivery (Cancer Alliances). Receipt of treatment also correlates with survival: 5 year Cancer survival varies from 28.6% to 49.6% across England. Here, we take a systems wide approach encompassing both diagnostic pathways and cancer treatment, derived from the whole cohort of women with ovarian cancer to set out recommendations and quality performance indicators (QPI). A multidisciplinary panel established by the British Gynaecological Cancer Society carefully identified QPI against criteria: metrics selected were those easily evaluable nationally using routinely available data and where there was a clear evidence base to support interventions. These QPI will be valuable to other taxpayer funded systems with national data collection mechanisms and are to our knowledge the only population level data derived standards in ovarian cancer. We also identify interventions for Best practice and Research recommendations

De Novo Variants in DENND5B Cause a Neurodevelopmental Disorder

The Rab family of guanosine triphosphatases (GTPases) includes key regulators of intracellular transport and membrane trafficking targeting specific steps in exocytic, endocytic, and recycling pathways. DENND5B (Rab6-interacting Protein 1B-like protein, R6IP1B) is the longest isoform of DENND5, an evolutionarily conserved DENN domain-containing guanine nucleotide exchange factor (GEF) that is highly expressed in the brain. Through exome sequencing and international matchmaking platforms, we identified five de novo variants in DENND5B in a cohort of five unrelated individuals with neurodevelopmental phenotypes featuring cognitive impairment, dysmorphism, abnormal behavior, variable epilepsy, white matter abnormalities, and cortical gyration defects. We used biochemical assays and confocal microscopy to assess the impact of DENND5B variants on protein accumulation and distribution. Then, exploiting fluorescent lipid cargoes coupled to high-content imaging and analysis in living cells, we investigated whether DENND5B variants affected the dynamics of vesicle-mediated intracellular transport of specific cargoes. We further generated an in silico model to investigate the consequences of DENND5B variants on the DENND5B-RAB39A interaction. Biochemical analysis showed decreased protein levels of DENND5B mutants in various cell types. Functional investigation of DENND5B variants revealed defective intracellular vesicle trafficking, with significant impairment of lipid uptake and distribution. Although none of the variants affected the DENND5B-RAB39A interface, all were predicted to disrupt protein folding. Overall, our findings indicate that DENND5B variants perturb intracellular membrane trafficking pathways and cause a complex neurodevelopmental syndrome with variable epilepsy and white matter involvement

Identification of Early Requirements for Preplacodal Ectoderm and Sensory Organ Development

Preplacodal ectoderm arises near the end of gastrulation as a narrow band of cells surrounding the anterior neural plate. This domain later resolves into discrete cranial placodes that, together with neural crest, produce paired sensory structures of the head. Unlike the better-characterized neural crest, little is known about early regulation of preplacodal development. Classical models of ectodermal patterning posit that preplacodal identity is specified by readout of a discrete level of Bmp signaling along a DV gradient. More recent studies indicate that Bmp-antagonists are critical for promoting preplacodal development. However, it is unclear whether Bmp-antagonists establish the proper level of Bmp signaling within a morphogen gradient or, alternatively, block Bmp altogether. To begin addressing these issues, we treated zebrafish embryos with a pharmacological inhibitor of Bmp, sometimes combined with heat shock-induction of Chordin and dominant-negative Bmp receptor, to fully block Bmp signaling at various developmental stages. We find that preplacodal development occurs in two phases with opposing Bmp requirements. Initially, Bmp is required before gastrulation to co-induce four transcription factors, Tfap2a, Tfap2c, Foxi1, and Gata3, which establish preplacodal competence throughout the nonneural ectoderm. Subsequently, Bmp must be fully blocked in late gastrulation by dorsally expressed Bmp-antagonists, together with dorsally expressed Fgf and Pdgf, to specify preplacodal identity within competent cells abutting the neural plate. Localized ventral misexpression of Fgf8 and Chordin can activate ectopic preplacodal development anywhere within the zone of competence, whereas dorsal misexpression of one or more competence factors can activate ectopic preplacodal development in the neural plate. Conversely, morpholino-knockdown of competence factors specifically ablates preplacodal development. Our work supports a relatively simple two-step model that traces regulation of preplacodal development to late blastula stage, resolves two distinct phases of Bmp dependence, and identifies the main factors required for preplacodal competence and specification