Situational awareness and safety

This paper considers the applicability of situation awareness concepts to safety in the control of complex systems. Much of the research to date has been conducted in aviation, which has obvious safety implications. It is argued that the concepts could be extended to other safety critical domains. The paper presents three theories of situational awareness: the three-level model, the interactive sub-systems approach, and the perceptual cycle. The difference between these theories is the extent to which they emphasise process or product as indicative of situational awareness. Some data from other studies are discussed to consider the negative effects of losing situational awareness, as this has serious safety implications. Finally, the application of situational awareness to system design, and training are presented

Systematic and Performance Tests of the Hard X-ray Polarimeter X-Calibur

Mentor: Matthias Beilicke From the Washington University Undergraduate Research Digest: WUURD, Volume 9, Issue 1, Fall 2013. Published by the Office of Undergraduate Research. Joy Zalis Kiefer Director of Undergraduate Research and Assistant Dean in the College of Arts & Sciences

Service Learning for the Port Jefferson History and Nature Center: Senior Capstone Forestry Course

A community partnership between the Collins Academy, Jefferson, Texas, and the Arthur Temple College of Forestry and Agriculture, Stephen F. Austin State University, Nacogdoches, Texas, developed a plan to manage the 38-acre Port Jefferson History and Nature Center, located on Big Cypress Bayou. Students used knowledge of the resources, GPS (global positioning systems), ArcGIS 10.4, presentations, and posters to complete the project. The reflection and feedback with the community partners continued throughout the project and as guidance for future work. This partnership instilled in participating students the value of incorporating service learning into development of professional relationships for continued cooperation in vegetation removal, construction of gardens for the monarch butterfly and the Neches River rose mallow, and trail modifications. The project received a service-learning award for engaged student learning

Use of fluorescent Fab/Ab complexes and IncuCyte live-cell analysis to dynamically track cell surface markers and cell populations in mixed cultures

Abstract Fluorescently-labeled antibodies are widely used for visualising cellular protein expression/distribution (e.g. immunocytochemistry) and immuno-phenotyping (e.g. flow cytometry). However, their applications are largely confined to end-point or short term (min-h) detection, and the cell processing and labeling steps that are required often perturb the biology of interest. To enable longer-term, fully kinetic applications of cell surface protein markers in living cells, we have developed a novel strategy based on fluorescently-labeled antibody fragments (Fabs) and IncuCyte live-cell analysis. An Fc-targeted anti-mouse Fab fragment conjugated to a green-emitting fluoroprobe (IncuCyte FabFluor-488) was used to tag Abs to surface markers (e.g. CD4, CD20, Her2) via a simple one-step, no-wash protocol. Addition of the corresponding FabFluor-488-Ab complex to living cells (e.g. Jurkats, RAMOS, SKOV-3) produced long lasting (up to 5d), specific and stable cellular fluorescence at relatively low Ab concentrations (1mg ml−1) that did not perturb cell morphology or growth (IncuCyte S3). To illustrate the application of this approach the method was used to (a) quantify upregulation of the checkpoint protein PDL-1 over 48h in IFN-gamma-treated MDA-MB231 and SKOV-3 and tumor cells, (b) identify cellular subsets in PBMCs, and (c) observe proximity and engagement of A549 target cells by CD-8 positive PBMCs in immune-cell killing experimentsThis FabFluor-488/IncuCyte method enables long-term tracking and quantification of cell surface protein expression and the ability to identify cell subsets in living cultures over time. This method should prove powerful in analyses on complex and advanced heterogeneous cell models.</jats:p

Toward a Learning/Instruction Process Model for Facilitating the Instructional Design Cycle

Abstract. Many instructional design models have been proposed and their benefits are evident. However, there is lack of a common and formal notation to describe the product of the design. This causes difficulty in evaluating the product (the course) in the development. To eliminate the difficulty, we need a formal framework which has enough semantics for keeping the consistency of the product. Thus, this work aims at proposing a unified modeling framework for learning and instruction based on ontologies that has the potential to support some phases of instructional design. Furthermore, we give an example of how one-to-one instruction and collaborative learning are modeled on the proposed framework