Detecting groundwater discharge dynamics from point-to-catchment scale in a lowland stream : Combining hydraulic and tracer methods

Acknowledgements. We would like to thank members of the Northern Rivers Institute, Aberdeen University, for helpful discussions of data. We also thank Lars Rasmussen, Jolanta Kazmierczak and Charlotte Ditlevsen for help in the field. This study is part of the Hydrology Observatory, HOBE (http://www.hobe.dk), funded by the Villum Foundation and was as well funded by the Aarhus University Research Foundation.Peer reviewedPublisher PD

Subsurface nitrate reduction under wetlands takes place in narrow superficial zones

This study aims to investigate the depth distribution of the Nitrate Reduction Potential (NRP) on a natural and a re-established wetland. The obtained NRP provides a valuable data of the driving factors affecting denitrification, the Dissimilatory Nitrate Reduction to Ammonium (DNRA) process and the performance of a re-established wetland. Intact soil cores were collected and divided in slices for the determination of Organic Matter (OM) through Loss of Ignition (LOI) as well as Dissolved Organic Carbon (DOC) and NRP spiking nitrate in batch tests. The Nitrate Reduction (NR) was fitted as a pseudo-first order rate constant (k) from where NRPs were obtained. NR took place in a narrow superficial zone showing a dropping natural logarithmic trend along depth. The main driving factor of denitrification, besides depth, was OM. Although, DOC and LOI could not express by themselves and absolute correlation with NRP, high amounts of DOC ensured enough quantity and quality of labile OM for NR. Besides, high concentration of LOI but a scarce abundance of DOC failed to drive NR. DNRA was only important in superficial samples with high contents of OM. Lastly, the high NRP of the re-established wetland confirms that wetlands can be restored satisfactorily.Preprin

Groundwater–surface water interaction in Denmark

The study of groundwater–surface water interaction has attracted growing interest among researchers in recent years due to its wide range of implications from the perspectives of water management, ecology and contamination. Many of the studies shed light on conditions on a local scale only, without exploring a regional angle. To provide a broad and historical overview of groundwater–surface water interaction, a review of research carried out in Denmark was undertaken due to the high density of studies conducted in the country. The extent to which this topic has been investigated is related to Denmark's physiography and climate, the presence of numerous streams and lakes combined with shallow groundwater, and historical, funding, and administrative decisions. Study topics comprise groundwater detection techniques, numerical modeling, and contaminant issues including nutrients, ranging from point studies all the way to studies at national scale. The increase in studies in recent decades corresponds with the need to maintain the good status of groundwater-dependent ecosystems and protect groundwater resources. This review of three decades of research revealed that problems such as the difference in scales between numerical models and field observations, interdisciplinary research integrating hydrological and biological methods, and the effect of local processes in regional systems remain persistent challenges. Technical progress in the use of unmanned aerial vehicles, distributed temperature sensing, and new cost-effective methods for detecting groundwater discharge as well as the increasing computing capacity of numerical models emerge as opportunities for dealing with complex natural systems that are subject to modifications in future triggered by climate change.Next-Generation EU fundingPrograma Maria Zambrano Sénior (REF: MZSA03

Investigating the molecular mechanism of positive and negative allosteric modulators in the calcium-sensing receptor dimer

AbstractAllosteric modulators that are targeting the calcium-sensing receptor (CaSR) hold great therapeutic potential, and elucidating the molecular basis for modulation would thus benefit the development of novel therapeutics. In the present study, we aimed at investigating the mechanism of allosteric modulation in CaSR by testing dimers carrying mutations in the allosteric site of one or both of the subunits. To ensure measurements on a well-defined dimer composition, we applied a trans-activation system in which only the specific heterodimer of two loss-of-function mutants responded to agonist. Although one of these mutants was potentiated by a positive allosteric modulator, we showed that receptor activity was further potentiated in a trans-activation heterodimer containing a single allosteric site, however only when the allosteric site was located in the subunit responsible for G protein coupling. On the contrary, preventing activation in both subunits was necessary for obtaining full inhibition by a negative allosteric modulator. These findings correlate with the proposed activation mechanism of the metabotropic glutamate receptors (mGluRs), in which only a single transmembrane domain is activated at a time. CaSR and mGluRs belong to the class C G protein-coupled receptors, and our findings thus suggest that the activation mechanism is common to this subfamily.</jats:p

Evaluation of Temperature Profiling and Seepage Meter Methods for Quantifying Submarine Groundwater Discharge to Coastal Lagoons:Impacts of Saltwater Intrusion and the Associated Thermal Regime

Surface water-groundwater interactions were studied in a coastal lagoon performing 180 seepage meter measurements and using heat as a tracer in 30 locations along a lagoon inlet. The direct seepage meter measurements were compared with the results from analytical solutions for the 1D heat transport equation in three different scenarios: (1) Homogeneous bulk thermal conductivity (Ke); (2) horizontal heterogeneity in Ke; and (3) horizontal and vertical heterogeneity in Ke. The proportion of fresh groundwater and saline recirculated lagoon water collected from the seepage experiment was used to infer the location of the saline wedge and its effect on both the seepage meter results and the thermal regime in the lagoon bed, conditioning the use of the thermal methods. The different scenarios provided the basis for a better understanding of the underlying processes in a coastal groundwater-discharging area, a key factor to apply the best-suited method to characterize such processes. The thermal methods were more reliable in areas with high fresh groundwater discharge than in areas with high recirculation of saline lagoon water. The seepage meter experiments highlighted the importance of geochemical water sampling to estimate the origin of the exchanged water through the lagoon bed

Application of Stable Isotopes of Water to Study Coupled Submarine Groundwater Discharge and Nutrient Delivery

Submarine groundwater discharge (SGD)&mdash;including terrestrial freshwater, density-driven flow at the saltwater&ndash;freshwater interface, and benthic exchange&mdash;can deliver nutrients to coastal areas, generating a negative effect in the quality of marine water bodies. It is recognized that water stable isotopes (18O and 2H) can be helpful tracers to identify different flow paths and origins of water. Here, we show that they can be also applied when assessing sources of nutrients to coastal areas. A field site near a lagoon (Ringk&oslash;bing Fjord, Denmark) has been monitored at a metric scale to test if stable isotopes of water can be used to achieve a better understanding of the hydrochemical processes taking place in coastal aquifers, where there is a transition from freshwater to saltwater. Results show that 18O and 2H differentiate the coastal aquifer into three zones: Freshwater, shallow, and deep saline zones, which corresponded well with zones having distinct concentrations of inorganic phosphorous. The explanation is associated with three mechanisms: (1) Differences in sediment composition, (2) chemical reactions triggered by mixing of different type of fluxes, and (3) biochemical and diffusive processes in the lagoon bed. The different behaviors of nutrients in Ringk&oslash;bing Fjord need to be considered in water quality management. PO4 underneath the lagoon exceeds the groundwater concentration inland, thus demonstrating an intra-lagoon origin, while NO3, higher inland due to anthropogenic activity, is denitrified in the study area before reaching the lagoon

Tracing the Spatial Distribution of Whole-Lake Exchange of Groundwater and Lake Water in Low-Hydraulic Gradient Systems Using delta O-18 and Electrical Conductivity and Uncertain End-Member Mixing Analysis

&delta;18O and electrical conductivity (EC) were used successfully to trace the spatial distribution of whole-lake groundwater-lake exchange for a small (four ha) groundwater-fed lake situated in a low relief and low hydraulic gradient area. The method relies on quick sampling of shallow groundwater, direct analysis of EC in the field, and relatively in-expensive analysis of &delta;18O in the laboratory. Ternary uncertain end-member mixing analysis (precipitation, groundwater, and lake water) quantified the composition of water discharging to and recharging from the lake. The tracer distribution and mixing analysis were in agreement with the interpreted groundwater flow near the lake. The use of only one tracer (either &delta;18O or EC) gave the same results for the recharge segments, but the discharge segments changed the origin of the water from being groundwater to precipitation controlled. The two tracers complemented each other, especially with different signals in precipitation and groundwater. The uncertain end-members were assessed based on local (groundwater and lake water) and off-site (precipitation) data. The off-site data were found to be useful if it contained representative information on local-site seasonality (uncertainty, variance). Final end-member concentrations could explain the transience of the hydrology at the site (i.e., flooding of the area adjacent to the lake during periods with high precipitation, and variability of the &delta;18O signal in precipitation). This methodology potentially represents a new option to study groundwater-lake systems. The tracer information collected over only two days is useful by itself for developing the next steps like the quantification of fluxes based on other standard methods (Darcy approach, seepage meters, or temperature). The tracer information can provide quantitative estimation of inputs and outputs by using the mixing analysis

Languages' impact on emotional classification methods

There is currently a lack of research concerning whether Emotional Classification (EC) research on a language is applicable to other languages. If this is the case then we can greatly reduce the amount of research needed for different languages. Therefore, we propose a framework to answer the following null hypothesis: The change in classification accuracy for Emotional Classification caused by changing a single preprocessor or classifier is independent of the target language within a significance level of p = 0.05. We test this hypothesis using an English and a Danish data set, and the classification algorithms: Support-Vector Machine, Naive Bayes, and Random Forest. From our statistical test, we got a p-value of 0.12852 and could therefore not reject our hypothesis. Thus, our hypothesis could still be true. More research is therefore needed within the field of cross-language EC in order to benefit EC for different languages