Amplitude-equation formalism for four-wave-mixing geometry with transmission gratings

An amplitude equation is derived for a four-wave-mixing geometry with nearly counterpropagating, mutually incoherent, nondiffracting pump beams, spatially overlapping in a photorefractive material with a nonlocal response. This equation extends the earlier linear two-dimensional theory to the weakly nonlinear regime. The analysis also starts from a more complete equation for the photorefractive effect, which leads to the prediction of novel effects especially apparent in the nonlinear regime. Precise predictions for the spatiotemporal behavior of the grating amplitude in the nonlinear regime are presented. The range of validity of the amplitude equation is studied. The characteristics of the instability in the nonlinear regime are analyzed through a front-selection analysis

Temporal evolution of photorefractive double phase-conjugate mirrors

We present wave-optics calculations of the temporal and spatial evolution from random noise of a double phase-conjugate mirror in photorefractive media that show its image exchange and phase-reversal properties. The calculations show that for values of coupling coefficient times length greater than two the process exhibits excellent conjugation fidelity, behaves as an oscillator, and continues to operate even when the noise required for starting it is set to zero. For values less than two, the double phase-conjugation process exhibits poor fidelity and disappears when the noise is set to zero

Transition to an oscillator for double phase-conjugate mirror

Summary form only given. Some of the novel quantified characteristics for double phase conjugate mirrors are analysed including the effects of the nonlinearity on the critical dynamics (approach to saturation) and on the spatial distribution of the grating (large scale distortion of the beams and conjugation fidelity) and sensitivity to noise (seeding). The approach used also clarifies the question of linear instability and predicts a new transition to an oscillatory regime

Double phase conjugation

We model the double phase-conjugate mirror (DPCM) as a function of time, the average direction of propagation of the two beams forming the DPCM, and one transverse coordinate. Calculations show that the conjugation fidelity and reflectivity have different dependencies on the photorefractive coupling coefficient times length; the fidelity turns on abruptly with a threshold, whereas the reflectivity increases smoothly. The DPCM behaves as an oscillator at and above threshold: the time required for the reflectivity to reach the steady state dramatically slows down near threshold (like critical slowing down in lasers); above threshold the DPCM is self-sustaining even if the random noise terms used to start the process are set to zero. A decrease in the noise level improves the fidelity but increases the response time. The use of unbalanced input beam ratios results in asymmetric conjugation such that the fidelity obtained on the side of the weaker input beam is significantly reduced. The slowing down diminishes with increasing noise level or unbalanced input intensities

Differences in telomere length between sporadic and familial cutaneous melanoma

BACKGROUND: Several pieces of evidence indicate that a complex relationship exists between constitutional telomere length (TL) and the risk of cutaneous melanoma. Although the general perception is that longer telomeres increase melanoma risk, some studies do not support this association. We hypothesise that discordant data are due to the characteristics of the studied populations. OBJECTIVES: To evaluate the association of telomere length with familial and sporadic melanoma. METHODS: TL was measured by multiplex quantitative PCR in leukocytes from 310 melanoma patients according to familial/sporadic and single/multiple cancers and 216 age-matched controls. RESULTS: Patients with sporadic melanoma were found to have shorter telomeres as compared to those with familial melanoma. In addition, shorter telomeres, while tending to reduce the risk of familial melanoma regardless of single or multiple tumors, nearly trebled the risk of single sporadic melanoma. CONCLUSIONS: This is the first time that TL has been correlated to opposite effects on melanoma risk according to the presence or absence of familial predisposition. Individual susceptibility to melanoma should be taken into account when assessing the role of TL as a risk factor. This article is protected by copyright. All rights reserved

Acid-Labile Traceless Click Linker for Protein Transduction

Intracellular delivery of active proteins presents an interesting approach in research and therapy. We created a protein transduction shuttle based on a new traceless click linker that combines the advantages of click reactions with implementation of reversible pH-sensitive bonds. The azidomethyl-methylmaleic anhydride (AzMMMan) linker was found compatible with different click chemistries, demonstrated in bioreversible protein modification with dyes, polyethylene glycol, or a transduction carrier. Linkages were stable at physiological pH but reversible at the mild acidic pH of endosomes or lysosomes. We show that pH-reversible attachment of a defined endosome-destabilizing three-arm oligo(ethane amino)amide carrier generates an effective shuttle for protein delivery. The cargo protein nlsEGFP, when coupled via the traceless AzMMMan linker, experiences efficient cellular uptake and endosomal escape into the cytosol, followed by import into the nucleus. In contrast, irreversible linkage to the same shuttle hampers nuclear delivery of nlsEGFP which after uptake remains trapped in the cytosol. Successful intracellular delivery of bioactive ß-galactosidase as a model enzyme was also demonstrated using the pH-controlled shuttle system

(En)gendering the political: Citizenship from marginal spaces

This introduction sets out the central concerns of this special issue, the relationship between marginality and the political. In doing so it makes the argument that the process of marginalisation, the sites and experiences of ‘marginality’ provide a different lens through which to understand citizenship. Viewing the political as the struggle over belonging it considers how recent studies of citizenship have understood political agency. It argues that marginality can help us understand multiple scales, struggles and solidarities both within and beyond citizenship. Whilst there is a radical potential in much of the existing literature in citizenship studies it is also important to consider political subjectivities and acts which are not subsumed by right claims. Exploring marginality in this way means understanding how subjects are disenfranchised by regimes of citizenship and at the same how time this also (en)genders new political possibilities which are not always orientated towards 'inclusion'. The introduction then sets out how each article contributes to this project

Clinicopathological Profile and Surgical Treatment of Abdominal Tuberculosis: A Single Centre Experience in Northwestern Tanzania.

Abdominal tuberculosis continues to be a major public health problem worldwide and poses diagnostic and therapeutic challenges to general surgeons practicing in resource-limited countries. This study was conducted to describe the clinicopathological profile and outcome of surgical treatment of abdominal tuberculosis in our setting and compare with what is described in literature. A prospective descriptive study of patients who presented with abdominal tuberculosis was conducted at Bugando Medical Centre (BMC) in northwestern Tanzania from January 2006 to February 2012. Ethical approval to conduct the study was obtained from relevant authorities. Statistical data analysis was performed using SPSS version 17.0. Out of 256 patients enrolled in the study, males outnumbered females. The median age was 28 years (range = 16-68 years). The majority of patients (77.3%) had primary abdominal tuberculosis. A total of 127 (49.6%) patients presented with intestinal obstruction, 106 (41.4%) with peritonitis, 17 (6.6%) with abdominal masses and 6 (2.3%) patients with multiple fistulae in ano. Forty-eight (18.8%) patients were HIV positive. A total of 212 (82.8%) patients underwent surgical treatment for abdominal tuberculosis. Bands /adhesions (58.5%) were the most common operative findings. Ileo-caecal region was the most common bowel involved in 122 (57.5%) patients. Release of adhesions and bands was the most frequent surgical procedure performed in 58.5% of cases. Complication and mortality rates were 29.7% and 18.8% respectively. The overall median length of hospital stay was 32 days and was significantly longer in patients with complications (p < 0.001). Advanced age (age ≥ 65 years), co-morbid illness, late presentation, HIV positivity and CD4+ count < 200 cells/μl were statistically significantly associated with mortality (p < 0.0001). The follow up of patients were generally poor as only 37.5% of patients were available for follow up at twelve months after discharge. Abdominal tuberculosis constitutes a major public health problem in our environment and presents a diagnostic challenge requiring a high index of clinical suspicion. Early diagnosis, early anti-tuberculous therapy and surgical treatment of the associated complications are essential for survival

4-Phenylbutyric acid treatment rescues trafficking and processing of a mutant surfactant protein C

Mutations in the SFTPC gene, encoding surfactant protein–C (SP-C), are associated with interstitial lung disease (ILD). Knowledge of the intracellular fate of mutant SP-C is essential in the design of therapies to correct trafficking/processing of the proprotein, and to prevent the formation of cytotoxic aggregates. We assessed the potential of a chemical chaperone to correct the trafficking and processing of three disease-associated mutant SP-C proteins. HEK293 cells were stably transfected with wild-type (SP-C(WT)) or mutant (SP-C(L188Q), SP-C(Δexon4), or SP-C(I73T)) SP-C, and cell lines with a similar expression of SP-C mRNA were identified. The effects of the chemical chaperone 4-phenylbutyric acid (PBA) and lysosomotropic drugs on intracellular trafficking to the endolysosomal pathway and the subsequent conversion of SP-C proprotein to mature peptide were assessed. Despite comparable SP-C mRNA expression, proprotein concentrations varied greatly: SP-C(I73T) was more abundant than SP-C(WT) and was localized to the cell surface, whereas SP-C(Δexon4) was barely detectable. In contrast, SP-C(L188Q) and SP-C(WT) proprotein concentrations were comparable, and a small amount of SP-C(L188Q) was localized to the endolysosomal pathway. PBA treatment restored the trafficking and processing of SP-C(L188Q) to SP-C(WT) concentrations, but did not correct the mistrafficking of SP-C(I73T) or rescue SP-C(Δexon4). PBA treatment also promoted the aggregation of SP-C proproteins, including SP-C(L188Q). This study provides proof of the principle that a chemical chaperone can correct the mistrafficking and processing of a disease-associated mutant SP-C proprotein