Supkutana ektopija pluća u vratnom području u teleta - prikaz slučaja.

This case presents a clinical, radiological and histopathological description of ectopic lung in a calf. The present case was a 45-day-old, female Simmental calf, showing a congenital mass in the lower neck region. Upon clinical inspection, it was determined that the calf was in excellent condition, but a pedunculated tumorlike mass appeared in the neck region. Palpation revealed that the mass had normal body temperature and was somewhat hard and flexible. The mass was surgically removed under inhalation anesthesia and diagnosed as an ectopic lung based on the histopathological examination. The calf was monitored for two years after the operation and was observed to be healthy and better developed than its peers. We concluded that a rare case like this should be presented as a contribution to the literature and veterinary practice.Prikazan je klinički, radiološki i histopatološki opis ektopije pluća u teleta. U ženskog teleta simentalske pasmine, u dobi od 45 dana, primijećena je kongenitalna masa u ventralnom području vrata. Tele je klinički bilo u odličnoj kondiciji s visećom masom nalik na tumor u području vrata. Palpacijom je ustanovljena masa normalne tjelesne temperature, ponešto tvrđa i pomična. Masa je kirurški bila uklonjena pod inhalacijskom anestezijom. Dijagnosticirana je ektopija pluća na osnovi histopatološke pretrage. Tele je bilo promatrano tijekom dvije godine nakon operacije. Ostalo je zdravo i bolje se razvijalo od ostalih iste dobi. Prikaz ovog slučaja koristan je prilog literaturi i veterinarskoj praksi

Uloga oksidacijskog i nitrozativnog stresa kod skvamocelularnih karcinoma oka u goveda

Oxidative stress is strongly linked to carcinogenesis, especially head, neck and oral SCCs in humans. In this study, we aimed to evaluate the role of oxidative and nitrosative stress in Bovine ocular squamous cell carcinomas (BOSCCs) using immunohistochemical markers such as Nitrotyrosine (NT), Malondialdehyde (MDA) and 8-hydroxy-2’ -deoxyguanosine (8-OHdG). Tissues were collected from 24 cattle brought to the Pathology Department for routine diagnosis. Tumour samples were fixed in 10% neutral buffered formalin, processed routinely, and embedded in paraffin wax. Tissue sections were cut into 5 μm thickness and stained with Hematoxylin-Eosin to detect histopathological changes. The sections were examined under a light microscope and photographed. The avidin-biotin-peroxidase method was used as immunohistochemical staining. It was observed that the cauliflower-like tumoral masses were mostly located on the upper and lower eyelids, the third eyelid, cornea, limbus, or covering the entire eyeball. NT, MDA and 8-OHdG expressions were statistically increased in poorly-differentiated (PD) cases compared to welldifferentiated (WD) and moderately-differentiated (MD) cases. On the basis of the results of the increase in these markers, we concluded that nitrosative and oxidative stress may have an important role in the carcinogenesis of BOSCCs.Oksidacijski je stres snažno povezan s karcinogenezom, osobito kad je riječ o skvamocelularnom karcinomu (SCC) u području glave, vrata i usne šupljine u ljudi. Cilj je istraživanja bio procijeniti ulogu oksidacijskog i nitrozativnog stresa kod skvamocelularnih karcinoma oka u goveda (BOSCCs) upotrebom imunohistokemijskih biljega kao što su nitrotirozin (NT), malondialdehid (MDA) i 8-hidroksi-2›-deoksigvanozin (8-OHdG). Prikupljena su tkiva 24 goveda i dopremljena na Odjel za patologiju radi rutinske dijagnostike. Uzorci tumora fiksirani su u 10%-tnom neutralnom puferiranom formalinu, rutinski obrađeni i ugrađeni u parafinski vosak. Dijelovi tkiva rezani su na debljinu od 5 μm i obojeni hematoksilin-eozin bojenjem kako bi se otkrile histopatološke promjene. Rezovi su pregledani svjetlosnim mikroskopom i fotografirani. Za imunohistokemijsko bojenje primijenjena je metoda avidin-biotinske peroksidaze. Uočeno je da su tumorske tvorbe nalik na cvjetaču većinom smještene na gornjem i donjem kapku, na trećem kapku, rožnici i limbusu, ili su prekrivale cijelu očnu jabučicu. Izražaji NT, MDA i 8-OHdG bili su statistički znakovito povećani u slabo diferenciranim (PD) slučajevima u usporedbi s dobro diferenciranim (WD) i umjereno diferenciranim (MD) slučajevima. Na temelju povećanih vrijednosti ovih biljega zaključili smo da nitrozativni i oksidacijski stres mogu imati važnu ulogu u karcinogenezi skvamoznih stanica oka u goveda

Sarkoid donjeg očnog kapka kod magarca (Equus asinus) izazvan bovinim papiloma 2 virusom

Sarcoid tumors were described by means of histopathological and molecular procedures in a 5-year-old donkey. Histopathological examination showed epithelial changes including hyperkeratosis, epithelial hyperplasia, koilocytosis, and rete peg formation. Neoplastic fibroblastic cells were plumb, large spindle to stellate and embedded in dense collagenous tissue. Results of Polymerase Chain Reaction and DNA sequence analysis showed that the etiological agent belonged to Bovine Papilloma Virus-II species in the delta papilloma virus genus. This case study represents the first report demonstrating the presence of Bovine Papilloma Virus-II in donkey sarcoid.Sarkoidi su opisani primenom histopatoloških i molelularanih metoda i procedura kod magarca, starog pet godina. Histopatološko ispitivanje, ukazalo je na promene u epitelu koje su uključivale hiperkeratozu, hiperplaziju epitela, koilocitozu i formiranje invaginacija epitela ptema dubokom dermisu - rete peg. Fibroblastine neoplastične ćelije su bile krupne, ovalnovretenaste i zvezdaste, uronjene u tkivo bogato kolagenom. Ispitivanje PCR metodom kao i rezultati DNK sekvencioniranja pokazali su da je etiološki agens spadao u grupu bovinog papilomavirusa-2 koji pripada rodu delta papilomavirusa. Ova studija predstavlja prvi opisani slučaj prisustva papiloma virusa goveda-2 kod sarkoida magarca

Immunohistochemical assessment of mda and 8-ohdg expression in the skin, lungs and kidneys of lambs naturally infected with sheeppox virus confirmed with pcr

This study aimed to assess the role of free radicals in the pathogenesis and progression of sheeppox with the immunohistochemical investigation of MDA and 8-OhdG expressions. The study material comprised of 24 lamb carcasses (suspected of being infected with sheeppox), which were referred to the Pathology Department for routine diagnosis, and 6 healthy lambs used for control purposes. Commercial MDA and 8-OHdG were used for labeling with the avidin-biotin-Peroxidase complex technique by the manufacturer"s instructions. By using PCR the correct-sized amplicon was obtained from eight of the samples. Characteristic pock nodules were detected in the skin, lung, and liver tissues. In histopathological examinations, sheeppox cells and Guarneri bodies, which are quite typical for the diagnosis of the disease, were found in the internal organs. All of the sheeppox-infected cases yielded positive results for MDA and 8-OHdG immunostainings. When compared to the control group, the sheeppoxinfected group displayed statistically higher levels of MDA and 8-OHdG expressions. In conclusion, increased MDA and 8-OHdG expressions in the visceral organs of lambs naturally infected with the sheeppox virus demonstrated that sheeppox is associated with the disruption of the antioxidant/oxidant balance and the occurrence of significant oxidative stress-induced damage to macromolecules such as lipids and the DNA. The positive correlation detected between the severity of the disease and the expression of these biomarkers showed that free radicals are actively involved in the development of pox lesions

Expression of PCNA, MMP-9 and P53 in bovine ocular squamous cell carcinomas: An immunohistochemical study

In this study, we aimed to compare and correlate the PCNA, MMP-9 and p53 expressions and differentiation degree of bovine ocular squamous cell carcinomas (BOSCCs) by immunohistochemical methods. The material of this study was composed of BOSCC biopsy samples taken from 30 cattle brought to our department. Tissue samples were fixed in 10% buffered formalin, processed routinely, embedded in paraffin, sectioned at 5 μm, and stained with hematoxylin & eosin in order to detect histopathological changes. Sections were examined and photographed under a light microscope. Avidin-biotin-peroxidase method was used for immunohistochemistry. Macroscopically the masses were nodular to cauliflower-like shaped. The surfaces of the masses were highly hemorrhagic and ulcerative, and sometimes covered with a purulent discharge. Histopathologically, we defined cases with excessive and large numbers of keratin pearls, large tumoral islands, and evident squamous differentiation as well-differentiated. Cases with smaller tumoral islets, decreased number and size of keratin pearls, but higher number of poorly-differentiated cells compared to well-differentiated cases were defined as moderately-differentiated. Tumors in which keratinization was either absent or formed in individual cells were classified as poorly-differentiated. Statistical analysis revealed that there was no significant difference between well, moderately, and poorly differentiated tumors in terms of PCNA and MMP-9 expressions, but we found that the increase in p53 expression negatively correlated with the degree of differentiation of the tumor. In conclusion, we think that p53 can be a useful marker in determining the prognosis of BOSCCs

Apoptosis in bovine ocular squamouse cell carcinomas

Aim: Cell death, especially apoptosis, is one of the most studied topics regarding cells. Understanding the mechanism of apoptosis in diseases will help understand the pathogenesis of diseases, as well as the treatment. This study aims to grade the Bovine ocular squamous cell carcinoma (BOSCC) and to evaluate apoptosis in tumors with different anaplastic features. Materials and Methods: A total of 30 formalin-fixed tumor tissues consisting of 10 poorly-differentiated, 10 moderately-differentiated, and 10 well-differentiated tissues were used to examine apoptosis in BOSCCs. Hematoxylin & eosin (HE) was performed for histopathological examination and TUNEL staining was performed for detection of apoptosis in all BOSCCs. Results: It was determined that apoptosis decreased as the malignancy increased in BOSCCs (p<0.001). Although all the tumors examined were malignant, apoptosis, i.e. controlled cell death, was found to be severe in well-differentiated type and mild in moderate and poorly-differentiated types. Conclusion: It was concluded that this case observed in bovines should be considered in studies on the pathogenesis of cancer and treatment

The immunohistochemical evaluation of the expression of intermediary filaments, pcna, p53 and mmp-9 in feline fibrosarcomas

Aim: The present study was aimed at the immunohistochemical evaluation of the expression of cancer indicators; including the Proliferating Cell Nucleus Antigen (PCNA), Matrix Metalloproteinase-9 (MMP-9), vimentin, alphasmooth muscle actin (?-SMA), S-100, desmin and pan-Cytokeratin (Pan- CK), as well as intermediary filaments, with a view to demonstrate the cell proliferation index, p53 tumour-suppressor gene status, tumour metastasis, invasion capacity and cellular origin of feline fibrosarcomas. Materials and Methods: The study material comprised of tumoral tissue samples from 7 cats, which were referred to the Pathology Department for routine histopathological examination. In order to evaluate the histopathological changes, the sections were stained with Haematoxylin and Eosin. Streptavidin-biotin peroxidase complex method was used for immunohistochemical staining. Results: Out of the seven spontaneous cutaneous fibrosarcoma cases, five were in stage I, one was in stage II, and one was in stage III. The presence of conspicuous collagen bands in the tumoral tissues was demonstrated by Masson's trichrome (MT) staining. All cases stained positively for vimentin and ?-SMA. The tumoral cells reacted negatively for pan-CK, S-100 and desmin. All cases were immunopositive for PCNA. Two cases stained positively for p53. These two cases were advanced stage. Only a single case of fibrosarcoma (stage III) presented with intracytoplasmic MMP-9 expression in neoplastic giant cells. Conclusion: Despite only very few tumour samples having been examined in the present study, it was concluded that PCNA, p53 and MMP-9 served as highly useful indicators for determining cell proliferation, grading malignancy, detecting tumour aggressiveness and behaviour, as well as predicting prognosis in spontaneous feline fibrosarcomas