Native People's Rights

Ce sont de facteurs sociaux, économiques et politiques que dépend la survie des populations indigènes. La protection de certains droits est considérée de première importance. L'auteur conclut dans cette optique qu'il est primordial d'accéder à une reconnaissance effective des droits des peuples indigènes et cela, par l'intermédiaire de garanties constitutionnelles et de conventions internationales. Les peuples indigènes constituent une force vitale et doivent être traités de façon à ce qu'ils se réalisen

C6orf10 low-frequency and rare variants in italian multiple sclerosis patients

In light of the complex nature of multiple sclerosis (MS) and the recently estimated contribution of low-frequency variants into disease, decoding its genetic risk components requires novel variant prioritization strategies. We selected, by reviewing MS Genome Wide Association Studies (GWAS), 107 candidate loci marked by intragenic single nucleotide polymorphisms (SNPs) with a remarkable association (p-value <= 5 x 10(-6)). A whole exome sequencing (WES)-based pilot study of SNPs with minor allele frequency (MAF) <= 0.04, conducted in three Italian families, revealed 15 exonic low-frequency SNPs with affected parent-child transmission. These variants were detected in 65/120 Italian unrelated MS patients, also in combination (22 patients). Compared with databases (controls gnomAD, dbSNP150, ExAC, Tuscany-1000 Genome), the allelic frequencies of C6orf10 rs 16870005 and IL2RA rs12722600 were significantly higher (i.e., controls gnomAD, p = 9.89 x 10(-7) and p < 1 x 10(-20)). TET2 rs61744960 and TRAF3 rs138943371 frequencies were also significantly higher, except in Tuscany-1000 Genome. Interestingly, the association of C6orf10 rs16870005 (Ala431Thr) with MS did not depend on its linkage disequilibrium with the HLA-DRB1 locus. Sequencing in the MS cohort of the C6orf10 3' region revealed 14 rare mutations (10 not previously reported). Four variants were null, and significantly more frequent than in the databases. Further, the C6orf10 rare variants were observed in combinations, both intra-locus and with other low-frequency SNPs. The C6orf10 Ser389Xfr was found homozygous in a patient with early onset of the MS. Taking into account the potentially functional impact of the identified exonic variants, their expression in combination at the protein level could provide functional insights in the heterogeneous pathogenetic mechanisms contributing to MS.In light of the complex nature of multiple sclerosis (MS) and the recently estimated contribution of low-frequency variants into disease, decoding its genetic risk components requires novel variant prioritization strategies. We selected, by reviewing MS Genome Wide Association Studies (GWAS), 107 candidate loci marked by intragenic single nucleotide polymorphisms (SNPs) with a remarkable association (p-value ≤ 5 × 10−6). A whole exome sequencing (WES)-based pilot study of SNPs with minor allele frequency (MAF) ≤ 0.04, conducted in three Italian families, revealed 15 exonic low-frequency SNPs with affected parent-child transmission. These variants were detected in 65/120 Italian unrelated MS patients, also in combination (22 patients). Compared with databases (controls gnomAD, dbSNP150, ExAC, Tuscany-1000 Genome), the allelic frequencies of C6orf10 rs16870005 and IL2RA rs12722600 were significantly higher (i.e., controls gnomAD, p = 9.89 × 10−7 and p < 1 × 10−20). TET2 rs61744960 and TRAF3 rs138943371 frequencies were also significantly higher, except in Tuscany-1000 Genome. Interestingly, the association of C6orf10 rs16870005 (Ala431Thr) with MS did not depend on its linkage disequilibrium with the HLA-DRB1 locus. Sequencing in the MS cohort of the C6orf10 3′ region revealed 14 rare mutations (10 not previously reported). Four variants were null, and significantly more frequent than in the databases. Further, the C6orf10 rare variants were observed in combinations, both intra-locus and with other low-frequency SNPs. The C6orf10 Ser389Xfr was found homozygous in a patient with early onset of the MS. Taking into account the potentially functional impact of the identified exonic variants, their expression in combination at the protein level could provide functional insights in the heterogeneous pathogenetic mechanisms contributing to MS

Influence of pre-reproductive maternal enrichment on coping response to stress and expression of c-Fos and glucocorticoid receptors in adolescent offspring

Environmental enrichment (EE) is an experimental setting broadly used for investigating the effects of complex social, cognitive, and sensorimotor stimulations on brain structure and function. Recent studies point out that parental EE experience, even occurring in the pre-reproductive phase, affects neural development and behavioral trajectories of the offspring. In the present study we investigated the influences of pre-reproductive EE of female rats on maternal behavior and adolescent male offspring's coping response to an inescapable stressful situation after chronic social isolation. For this purpose female Wistar rats were housed from weaning to breeding age in enriched or standard environments. Subsequently, all females were mated and housed in standard conditions until offspring weaning. On the first post partum day (ppd 1), mother-pup interactions in undisturbed conditions were recorded. Further, after weaning the male pups were reared for 2 weeks under social isolation or in standard conditions, and then submitted or not to a single-session Forced Swim Test (FST). Offspring's neuronal activation and plastic changes were identified by immunohistochemistry for c-Fos and glucocorticoid receptors (GRs), and assessed by using stereological analysis. The biochemical correlates were measured in the hippocampus, amygdala and cingulate cortex, structures involved in hypothalamic-pituitary-adrenocortical axis regulation. Enriched dams exhibited increased Crouching levels in comparison to standard reared dams. In the offspring of both kinds of dams, social isolation reduced body weight, decreased Immobility, and increased Swimming during FST. Moreover, isolated offspring of enriched dams exhibited higher levels of Climbing in comparison to controls. Interestingly, in the amygdala of both isolated and control offspring of enriched dams we found a lower number of c-Fos immunopositive cells in response to FST and a higher number of GRs in comparison to the offspring of standard dams. These results highlight the profound influence of a stressful condition, such as the social isolation, on the brain of adolescent rats, and underline intergenerational effects of maternal experiences in regulating the offspring response to stress

La Comida y las Memorias: Food, Positionality, and the Problematics of Making One\u27s Home

This work explores how people talk about food. My original problem was to find how the idea of a cultural group one may see as comprised of Mexican Americans may or may not be complicated by a regional comparison (of rural Hispanos in Colorado and New Mexico and urban Mexicans and Mexican Americans in Philadelphia). The main question I asked myself in this exploration (as well as asked field consultants) was: How does food play a role in the enactment of self-definition? Out of a comparison of over 30 rural and urban food narratives gathered during fieldwork in both regions emerged the interesting link between food, place, and culture. These food narratives demonstrated a sense of place, a topophilia enacted through talking about food in one\u27s life. The men and women interviewed for this thesis use location and space to define and characterize (as well as to perform) the role of food in their lives, an action I call performative mapping. My conclusion was that the notion of home and of cultural borders expressed in narrative contrasts of us versus them, antes versus ahora, and here versus there are used by interviewees to perform authenticity as well as cultural boundary maintenance

Decreased VLDL-Apo B 100 fractional synthesis rate despite hypertriglyceridemia in subjects with type 2 diabetes and nephropathy

Subjects with Type 2 Diabetes Mellitus (T2DM) and diabetic nephropathy (DN) often exhibit hypertriglyceridemia. The mechanism(s) of such an increase are poorly known. OBJECTIVE: We investigated VLDL-Apo B 100 kinetics in T2DM subjects with and without DN, and in healthy controls. DESIGN: Stable isotope 13C-leucine infusion, and modelling analysis of tracer-to-tracee ratio dynamics in the protein product pool in the 6-8 hr period following tracer infusion, were employed. SETTING: Male subjects affected by T2DM, either with (n=9) or without (n=5) DN, and healthy male controls (n=6), were studied under spontaneous glycemic levels in the post-absorptive state. RESULTS: In the T2DM patients with DN, plasma triglyceride (TG) (2.2\ub10.8 mmol/L, Mean\ub1SD) and VLDL-Apo B 100 (17.4\ub110.4 mg/dl) concentrations, and VLDL-Apo B 100 pool (0.56\ub10.29 g), were 3e60-80% greater (p<0.05 or less) than those of the T2DM subjects without DN (TG: 1.4\ub10.5 mmol/L; VLDL-Apo B 100: 9.9\ub12.5 mg/dl; VLDL-Apo B 100 pool: 0.36\ub10.09 g), and 3e80-110% greater (p<0.04 or less) than those of nondiabetic controls (TG: 1.2\ub10.4 mmol/L; VLDL-Apo B 100: 8.2\ub11.7 mg/dl; VLDL-Apo B 100: 0.32\ub10.09 g). In sharp contrast however, in the subjects with T2DM and DN, VLDL-Apo B 100 FSR was 6550% lower (4.8\ub12.2 pools/day) than that of either the T2DM subjects without DN (9.9\ub14.3 pools/day, p<0.025) or the control subjects (12.5\ub19.1 pools/day, p<0.04). CONCLUSIONS: The hypertriglyceridemia of T2DM patients with DN is not due to hepatic VLDL-Apo B 100 overproduction, which is decreased, but it should be attributed to decreased apolipoprotein removal

Global environmental sustainability trends: A temporal comparison using a new interval-based composite indicator

Assessing progress on the pursuit of the Sustainable Development Goals is crucial for evaluating the sustainability of a Country, although this is not easy, considering the interdependencies or interconnections of individual goals with others, and the fact that there are several indicators for each goal. The aims of this research are: (1) to propose a novel interval-based environmental sustainable composite index (ESI) suitable to monitor the worldwide environmental SDGs' implementation at national scale, (2) to solve the problem of missing data in large databases and the subjectivity in computing a composite index (CI), (3) to group and compare statistically countries according to the ESI, and (4) to represent spatially the results to identify areas of the world more or less environmentally sustainable than others. Clustering and Sankey diagrams have supported the temporal and spatial analysis of ESI trends, showing that Canada, Brazil, New Zealand, and several European countries have been the most sustainable in 2019. The novelty of this indicator is that each country presents an ESI central value, the most probable value of the composite indicator, and a range, which represents the uncertainty given by the lower and upper bounds. In this sense, it is possible to better interpret the results of the composite indicator, while simultaneously obtaining a measure of the uncertainty of the results. The composite indicator can be used to monitor countries’ vulnerability towards the unsustainability risk, as well as countries that are not able to escape from a sort of “unsustainability trap”

Disulfide-linked allosteric modulators for multi-cycle kinetic control of DNA-based nanodevices

Nature employs sulfur switches, that is, redox-active disulfides, to kinetically control biological pathways in a highly efficient and reversible way. Inspired by this mechanism, we describe herein a DNA-based synthetic nanodevice that acts as a sulfur switch and can be temporally controlled though redox regulation. To do this, we rationally designed disulfide DNA strands (modulators) that hybridize to a ligand-binding DNA nanodevice and act as redox-active allosteric regulators inducing the nanodevice to release or load its ligand. Upon reduction, the allosteric modulator spontaneously de-hybridizes from the nanodevice and, as a result, its effect is transient. The system is reversible and has an unprecedented high tolerance to waste products and displays transient behavior for over 40 cycles without significant loss of efficiency. Kinetic control of DNA-based ligand-binding nanodevices through purely chemical reactions paves the way for temporal regulation of more complex chemical pathways

Integrated 3D Mapping and Diagnosis for the Structural Assessment of Architectural Heritage: Morano’s Parabolic Arch

: The architectural heritage of the 20th century is affected by several conservation problems in terms of material preservation, structural analysis, and reuse. Among these, material degradation and durability issues are the ones that have the most effect on the health state and, consequently, the survival of the constructions of the period. In order to conduct a proper analysis for preservation purposes, an interdisciplinary approach is necessary. The parabolic arch in Morano sul Po (Italy) is a reinforced concrete landmark in the Casale Monferrato area and is related to the industrial vocation of the territory, which is indissolubly linked to the cement production chain. The present paper reports the results of a non-destructive test campaign by a Politecnico di Torino multidisciplinary group, which combined acquisitions using different methods. The paper highlights the importance of a structured procedure to integrate different information coming from different techniques. The aim was to assess the health state of the structure and define the best procedures for building an information system based on the as-built modeling strategy, which could serve as the basis to provide conservation guidelines

Fostering the Resiliency of Urban Landscape through the Sustainable Spatial Planning of Green Spaces

none5siBackground: It has been recognized that urban green spaces play a crucial role in providing many landscape services. The research aimed at identifying the main knowledge gaps in this framework and to support urban planning, taking into account the spatial configuration of green areas through a pilot study area, and mapping urban landscape services. Methods: In this research, (1) a systematic review, analyzed through a network analysis; (2) an urban pilot study to map the Urban Green Index and, jointly, the spatial composition and configuration of urban green areas, through the integration of three landscape metrics; and (3) the mapping of Urban Landscape Services Index have been carried out. Results: The 37% of the reviewed articles focused on regulating services, while the network analysis identified four clusters. The total Urban Green Index was 26%, and some districts showed a percentage that surpassed it. The total overall Green Connectivity Index was 21%. Some districts were the best providers of landscape services. Conclusions: This research was in line with the EU Joint Science for Policy Report suggesting giving emphasis to the spatial pattern map of green spaces in European cities to provide spatial data available for decision-makers in relation to GI deployment.openDonatella Valente, María Victoria Marinelli, Erica Maria Lovello, Cosimo Gaspare Giannuzzi, Irene PetrosilloValente, Donatella; Marinelli, MARIA VICTORIA; Lovello, ERICA MARIA; Gaspare Giannuzzi, Cosimo; Petrosillo, Iren