A cloud robotics architecture for an emergency management and monitoring service in a smart cityenvironment

Cloud robotics is revolutionizing not only the robotics industry but also the ICT world, giving robots more storage and computing capacity, opening new scenarios that blend the physical to the digital world. In this vision new IT architectures are required to manage robots, retrieve data from them and create services to interact with users. In this paper a possible implementation of a cloud robotics architecture for the interaction between users and UAVs is described. Using the latter as monitoring agents, a service for fighting crime in urban environment is proposed, making one step forward towards the idea of smart cit

Quantitative real-time PCR and high-resolution melting (HRM) analysis for strain-specific monitoring of fluorescent pseudomonads used as biocontrol agents against soil-borne pathogens of food crops

Fluorescent pseudomonads colonizing roots of crop plants and producing antifungal metabolites are regarded as a reliable alternative to chemical fungicides against soil-borne phytopathogens. Key factors in successful pathogen control are presence and activity at the appropriate concentration, time, and place of biocontrol agents. Thus, quantification methods to monitor population dynamics are pivotal to the development of reliable application protocols. Real-time PCR is nowadays the most widespread cultureindependent technique for the detection and enumeration of different target sequences. Here, its implementation with high resolution melting analysis as a powerful tool to accurately discriminate microbial inoculants is discussed

Localization and subcellular association of Grapevine Pinot Gris Virus in grapevine leaf tissues

Despite the increasing impact of Grapevine Pinot gris disease (GPG-disease) worldwide, etiology about this disorder is still uncertain. The presence of the putative causal agent, the Grapevine Pinot Gris Virus (GPGV), has been reported in symptomatic grapevines (presenting stunting, chlorotic mottling, and leaf deformation) as well as in symptom-free plants. Moreover, information on virus localization in grapevine tissues and virus-plant interactions at the cytological level is missing at all. Ultrastructural and cytochemical investigations were undertaken to detect virus particles and the associated cytopathic effects in field-grown grapevine showing different symptom severity. Asymptomatic greenhouse-grown grapevines, which tested negative for GPGV by real time RT-PCR, were sampled as controls. Multiplex real-time RT-PCR and ELISA tests excluded the presence of viruses included in the Italian certification program both in field-grown and greenhouse-grown grapevines. Conversely, evidence was found for ubiquitous presence of Grapevine Rupestris Stem Pitting-associated Virus (GRSPaV), Hop Stunt Viroid (HSVd), and Grapevine Yellow Speckle Viroid 1 (GYSVd-1) in both plant groups. Moreover, in every field-grown grapevine, GPGV was detected by real-time RT-PCR. Ultrastructural observations and immunogold labelling assays showed filamentous flexuous viruses in the bundle sheath cells, often located inside membrane-bound organelles. No cytological differences were observed among field-grown grapevine samples showing different symptom severity. GPGV localization and associated ultrastructural modifications are reported and discussed, in the perspective of assisting management and control of the disease. \ua9 2017 The Author(s

Subject and Object Clitic Pronouns in Valdostan Francoprovençal:The Fenisan Variety

Subject clitics (SCLs) are a debated topic regarding their structural position and their categorial status. Accounting for a phenomenon he observed in some Francoprovencal varieties and that he calls `OCL-for-SCL\u27, Roberts (2015, 2018b) proposes that SCLs in Francoprovencal are the result of the morphophonological process of fission. Focusing on a particular variety of Francoprovencal (the Fenisan Francoprovencal, or FFP), and based on Roberts\u27 (2010) approach of cliticization as incorporation, this study shows that Roberts\u27 proposal on the nature of SCLs in Francoprovencal cannot account for the data in FFP. Relying on the behavior of subject and object clitics with lexical and functional verbs, I show that SCLs in FFP are better analyzed as syntactic objects base-generated in argumental position inside the vP/VP domain. FFP also provides new arguments for considering both the auxiliary and possessive verb have and both the auxiliary and copular verb be as functional verbs, as the clitics behave differently with these verbs contrary to lexical verbs. This study also shows that there can be a direct interface between syntax and phonology regarding the representation of clitic pronouns

Pre-Modern Bosom Serpents and Hippocrates' Epidemiae 5: 86: A Comparative and Contextual Folklore Approach

A short Hippocratic passage (Epidemiae 5: 86) might constitute the earliest Western surviving variant of the well-known narrative and experiential theme of snakes or other animals getting into the human body (motif B784, tale-type ATU 285B*). This paper aims: 1) to throw light on this ancient passage through a comparative folkloric analysis and through a philological-contextual study, with reference to modern and contemporary interpretations; and 2) to offer an examination of previous scholarly enquiries on the fantastic intrusion of animals into the human body. In medieval and post-medieval folklore and medicine, sleeping out in the field was dangerous: snakes and similar animals could, it was believed, crawl into the sleeper’s body through the ears, eyes, mouth, nostrils, anus and vagina. Comparative material demonstrates, meanwhile, that the thirsty snake often entered the sleeper’s mouth because of its love of milk and wine. I will argue that while Epidemiae 5: 86 is modelled on this long-standing legendary pattern, for which many interesting literary pre-modern (and modern) parallels exist, its relatively precise historical and cultural framework can be efficiently analysed. The story is embedded in a broad set of Graeco-Roman ideas and practices surrounding ancient beliefs about snakes and attitudes to the drinking of unmixed wine

Advances in Human Robot Interaction for Cloud Robotics applications

In this thesis are analyzed different and innovative techniques for Human Robot Interaction. The focus of this thesis is on the interaction with flying robots. The first part is a preliminary description of the state of the art interactions techniques. Then the first project is Fly4SmartCity, where it is analyzed the interaction between humans (the citizen and the operator) and drones mediated by a cloud robotics platform. Then there is an application of the sliding autonomy paradigm and the analysis of different degrees of autonomy supported by a cloud robotics platform. The last part is dedicated to the most innovative technique for human-drone interaction in the User’s Flying Organizer project (UFO project). This project wants to develop a flying robot able to project information into the environment exploiting concepts of Spatial Augmented Realit

Phloem cytochemical modification and gene expression following the recovery of apple plants from apple proliferation

Recovery of apple trees from apple proliferation was studied by combining ultrastructural, cytochemical, and gene expression analyses to possibly reveal changes linked to recovery-associated resistance. When compared with either healthy or visibly diseased plants, recovered apple trees showed abnormal callose and phloem-protein accumulation in their leaf phloem. Although cytochemical localization detected Ca2+ ions in the phloem of all the three plant groups, Ca2+ concentration was remarkably higher in the phloem cytosol of recovered trees. The expression patterns of five genes encoding callose synthase and of four genes encoding phloem proteins were analyzed by quantitative real-time reverse transcription- polymerase chain reaction. In comparison to both healthy and diseased plants, four of the above nine genes were remarkably upregulated in recovered trees. As in infected apple trees, phytoplasma disappear from the crown during winter, but persist in the roots, and it is suggested that callose synthesis/deposition and phloem-protein plugging of the sieve tubes would form physical barriers preventing the recolonization of the crown during the following spring. Since callose deposition and phloem-protein aggregation are both Ca2+-dependent processes, the present results suggest that an inward flux of Ca2+ across the phloem plasma membrane could act as a signal for activating defense reactions leading to recovery in phytoplasma-infected apple trees.L'articolo é disponibile sul sito dell'editore: http://www.apsjournals.apsnet.or

The intelligent industry of the future: A survey on emerging trends, research challenges and opportunities in Industry 4.0

Strongly rooted in the Internet of Things and Cyber-Physical Systems-enabled manufacturing, disruptive paradigms like the Factory of the Future and Industry 4.0 envision knowledge-intensive industrial intelligent environments where smart personalized products are created through smart processes and procedures. The 4th industrial revolution will be based on Cyber-Physical Systems that will monitor, analyze and automate business processes, transforming production and logistic processes into smart factory environments where big data capabilities, cloud services and smart predictive decision support tools are used to increase productivity and efficiency. This survey provides insights into the latest developments in these domains, and identifies relevant research challenges and opportunities to shape the future of intelligent manufacturing environments.status: publishe