The distinct roles of the nucleus and nucleus-cytoskeleton connections in three-dimensional cell migration

Cells often migrate in vivo in an extracellular matrix that is intrinsically three-dimensional (3D) and the role of actin filament architecture in 3D cell migration is less well understood. Here we show that, while recently identified linkers of nucleoskeleton to cytoskeleton (LINC) complexes play a minimal role in conventional 2D migration, they play a critical role in regulating the organization of a subset of actin filament bundles – the perinuclear actin cap - connected to the nucleus through Nesprin2giant and Nesprin3 in cells in 3D collagen I matrix. Actin cap fibers prolong the nucleus and mediate the formation of pseudopodial protrusions, which drive matrix traction and 3D cell migration. Disruption of LINC complexes disorganizes the actin cap, which impairs 3D cell migration. A simple mechanical model explains why LINC complexes and the perinuclear actin cap are essential in 3D migration by providing mechanical support to the formation of pseudopodial protrusions

ExoClock Project III: 450 new exoplanet ephemerides from ground and space observations

The ExoClock project has been created with the aim of increasing the efficiency of the Ariel mission. It will achieve this by continuously monitoring and updating the ephemerides of Ariel candidates over an extended period, in order to produce a consistent catalogue of reliable and precise ephemerides. This work presents a homogenous catalogue of updated ephemerides for 450 planets, generated by the integration of $\sim$18000 data points from multiple sources. These sources include observations from ground-based telescopes (ExoClock network and ETD), mid-time values from the literature and light-curves from space telescopes (Kepler/K2 and TESS). With all the above, we manage to collect observations for half of the post-discovery years (median), with data that have a median uncertainty less than one minute. In comparison with literature, the ephemerides generated by the project are more precise and less biased. More than 40\% of the initial literature ephemerides had to be updated to reach the goals of the project, as they were either of low precision or drifting. Moreover, the integrated approach of the project enables both the monitoring of the majority of the Ariel candidates (95\%), and also the identification of missing data. The dedicated ExoClock network effectively supports this task by contributing additional observations when a gap in the data is identified. These results highlight the need for continuous monitoring to increase the observing coverage of the candidate planets. Finally, the extended observing coverage of planets allows us to detect trends (TTVs - Transit Timing Variations) for a sample of 19 planets. All products, data, and codes used in this work are open and accessible to the wider scientific community.Comment: Recommended for publication to ApJS (reviewer's comments implemented). Main body: 13 pages, total: 77 pages, 7 figures, 7 tables. Data available at http://doi.org/10.17605/OSF.IO/P298

Gliese 12 b: A temperate Earth-sized planet at 12 pc ideal for atmospheric transmission spectroscopy

Recent discoveries of Earth-sized planets transiting nearby M dwarfs have made it possible to characterize the atmospheres of terrestrial planets via follow-up spectroscopic observations. However, the number of such planets receiving low insolation is still small, limiting our ability to understand the diversity of the atmospheric composition and climates of temperate terrestrial planets. We report the discovery of an Earth-sized planet transiting the nearby (12 pc) inactive M3.0 dwarf Gliese 12 (TOI-6251) with an orbital period ($P_{\rm{orb}}$) of 12.76 days. The planet, Gliese 12b, was initially identified as a candidate with an ambiguous $P_{\rm{orb}}$ from TESS data. We confirmed the transit signal and $P_{\rm{orb}}$ using ground-based photometry with MuSCAT2 and MuSCAT3, and validated the planetary nature of the signal using high-resolution images from Gemini/NIRI and Keck/NIRC2 as well as radial velocity (RV) measurements from the InfraRed Doppler instrument on the Subaru 8.2 m telescope and from CARMENES on the CAHA 3.5 m telescope. X-ray observations with XMM-Newton showed the host star is inactive, with an X-ray-to-bolometric luminosity ratio of $\log L_{\rm X}/L_{\rm bol} \approx -5.7$. Joint analysis of the light curves and RV measurements revealed that Gliese 12b has a radius of 0.96 $\pm$ 0.05 $R_\oplus$, a 3$\sigma$ mass upper limit of 3.9 $M_\oplus$, and an equilibrium temperature of 315 $\pm$ 6 K assuming zero albedo. The transmission spectroscopy metric (TSM) value of Gliese 12b is close to the TSM values of the TRAPPIST-1 planets, adding Gliese 12b to the small list of potentially terrestrial, temperate planets amenable to atmospheric characterization with JWST.Comment: 29 pages (20 pages in main body), 13 figures (10 figures in main body). Equal contributions from M. K. and A. F.. Accepted for Publication in ApJL at 2024 March 2

ExoClock Project. III. 450 New Exoplanet Ephemerides from Ground and Space Observations

The ExoClock project has been created to increase the efficiency of the Ariel mission. It will achieve this by continuously monitoring and updating the ephemerides of Ariel candidates, in order to produce a consistent catalog of reliable and precise ephemerides. This work presents a homogenous catalog of updated ephemerides for 450 planets, generated by the integration of ∼18,000 data points from multiple sources. These sources include observations from ground-based telescopes (the ExoClock network and the Exoplanet Transit Database), midtime values from the literature, and light curves from space telescopes (Kepler, K2, and TESS). With all the above, we manage to collect observations for half of the postdiscovery years (median), with data that have a median uncertainty less than 1 minute. In comparison with the literature, the ephemerides generated by the project are more precise and less biased. More than 40% of the initial literature ephemerides had to be updated to reach the goals of the project, as they were either of low precision or drifting. Moreover, the integrated approach of the project enables both the monitoring of the majority of the Ariel candidates (95%), and also the identification of missing data. These results highlight the need for continuous monitoring to increase the observing coverage of the candidate planets. Finally, the extended observing coverage of planets allows us to detect trends (transit-timing variations) for a sample of 19 planets. All the products, data, and codes used in this work are open and accessible to the wider scientific community

A study of low-energy guest phonon modes in clathrate-II NaxSi136 (x = 3, 23, and 24)

Single-crystal x-ray diffraction from clathrate-II NaxSi136 (x = 24) prepared by a new technique reveals the exceptionally large Na@Si-28 atomic displacement parameter (U-eq) is strongly temperature dependent, and can be attributed to low-energy rattling modes associated with the Na guest. Inelastic neutron scattering (INS) spectra collected from NaxSi136 powder specimens (x = 3, 23) confirm the presence of low-energy guest-derived phonon modes for Na@Si-28 and Na@Si-20. The lower energy Na@Si-28 rattler mode falls in the frequency range of the silicon host acoustic phonons, indicating the possibility for interaction with these phonons. The presence of these low-energy modes combined with the ability to controllably vary the guest content presents a unique opportunity for exploring the influence of guest-framework interactions on the lattice dynamics in intermetallic clathrates