Rollenspiel

Das Rollenspiel als Erhebungsmethode in der qualitativen Sozialforschung bietet, verbunden mit dem Auswertungsverfahren der tiefenhermeneutischen Textinterpretation, die Möglichkeit, kollektiv unbewusste Prozesse in Gruppen in ihrem Bedeutungsgehalt für die den Interaktionen zugrunde liegenden Muster zu erkennen und zu verstehen. Gruppendynamische Prozesse werden erfasst und das rollenspezifische Handeln der Gruppenteilnehmenden bezogen auf das jeweilige Erfahrungsfeld analysiert. In dem Beitrag werden die Durchführung der Rollenspiele sowie deren Dokumentation und die Auswertung der in den Rollenspielen erhobenen Daten vorgestellt, insbesondere anhand konkreter Anwendungsbeispiele die Dokumentations- und Auswertungsschritte praxisorientiert dargelegt und Limitationen der Einsatzes von Rollenspielen diskutiert

Stimulating Myocardial Regeneration with Periostin Peptide in Large Mammals Improves Function Post-Myocardial Infarction but Increases Myocardial Fibrosis

AIMS: Mammalian myocardium has a finite but limited capacity to regenerate. Experimentally stimulating proliferation of cardiomyocytes with extracellular regeneration factors like periostin enhances cardiac repair in rodents. The aim of this study was to develop a safe method for delivering regeneration factors to the heart and to test the functional and structural effects of periostin peptide treatment in a large animal model of myocardial infarction (MI). METHODS AND RESULTS: We developed a controlled release system to deliver recombinant periostin peptide into the pericardial space. A single application of this method was performed two days after experimental MI in swine. Animals were randomly assigned to receive either saline or periostin peptide. Experimental groups were compared at baseline, day 2, 1 month and 3 months. Treatment with periostin peptide increased the EF from 31% to 41% and decreased by 22% the infarct size within 12 weeks. Periostin peptide-treated animals had newly formed myocardium strips within the infarct scar, leading to locally improved myocardial function. In addition the capillary density was increased in animals receiving periostin. However, periostin peptide treatment increased myocardial fibrosis in the remote region at one week and 12 weeks post-treatment. CONCLUSION: Our study shows that myocardial regeneration through targeted peptides is possible. However, in the case of periostin the effects on cardiac fibrosis may limit its clinical application as a viable therapeutic strategy