Nanoplastics: From tissue accumulation to cell translocation into Mytilus galloprovincialis hemocytes. resilience of immune cells exposed to nanoplastics and nanoplastics plus Vibrio splendidus combination

Plastic litter is an issue of global concern. In this work Mytilus galloprovincialis was used to study the distribution and effects of polystyrene nanoplastics (PS NPs) of different sizes (50 nm, 100 nm and 1 mu m) on immune cells. Internalization and translocation of NPs to hemolymph were carried out by in vivo experiments, while endocytic routes and effects of PS NPs on hemocytes were studied in vitro. The smallest PS NPs tested were detected in the digestive gland and muscle. A fast and size-dependent translocation of PS NPs to the hemolymph was recorded after 3 h of exposure. The internalization rate of 50 nm PS NPs was lower when caveolae and clathrin endocytosis pathways were inhibited. On the other hand, the internalization of larger particles decreased when phagocytosis was inhibited. The hemocytes exposed to NPs had changes in motility, apoptosis, ROS and phagocytic capacity. However, they showed resilience when were infected with bacteria after PS NP exposure being able to recover their phagocytic capacity although the expression of the antimicrobial peptide Myticin C was reduced. Our findings show for the first time the translocation of PS NPs into hemocytes and how their effects trigger the loss of its functional parameters

Kinematic groups across the MW disc: insights from models and from the RAVE catalogue

With the advent of the Gaia data, the unprecedented kinematic census of great part of the Milky Way disc will allow us to characterise the local kinematic groups and new groups in different disc neighbourhoods. First, we show here that the models predict a stellar kinematic response to the spiral arms and bar strongly dependent on disc position. For example, we find that the kinematic groups induced by the spiral arm models change significantly if one moves only ~ 0.6 kpc in galactocentric radius, but ~ 2 kpc in azimuth. There are more and stronger groups as one approaches the spiral arms. Depending on the spiral pattern speed, the kinematic imprints are more intense in nearby vicinities or far from the Sun. Secondly, we present a preliminary study of the kinematic groups observed by RAVE. This sample will allow us, for the first time, to study the dependence on Galactic position of the (thin and thick) disc moving groups. In the solar neighbourhood, we find the same kinematics groups as detected in previous surveys, but now with better statistics and over a larger spatial volume around the Sun. This indicates that these structures are indeed large scale kinematic features.Comment: 4 pages, 3 figures, to appear in the proceedings of "Assembling the Puzzle of the Milky Way", Le Grand Bornand (April 17-22, 2011), C. Reyle, A. Robin, M. Schultheis (eds.

Adherence to and Invasion of Human Intestinal Cells by Arcobacter Species and Their Virulence Genotypes

The genus Arcobacter is composed of 17 species which have been isolated from various sources. Of particular interest are A. butzleri, A. cryaerophilus, and A. skirrowii, as these have been associated with human cases of diarrhea, the probable transmissionrroutes being through the ingestion of contaminated drinking water and food. To date, only limited studies of virulence traits in this genus have been undertaken. The present study used 60 Arcobacter strains isolated from different sources, representing 16 of the 17 species of the genus, to investigate their ability to adhere to and invade the human intestinal cell line Caco-2. In addition, the presence of five putative virulence genes (ciaB, cadF, cj1349, hecA, and irgA) was screened for in these strains by PCR. All Arcobacter species except A. bivalviorum and Arcobacter sp. strain W63 adhered to Caco-2 cells, and most species (10/16) were invasive. The most invasive species were A. skirrowii, A. cryaerophilus, A. butzleri, and A. defluvii. All invasive strains were positive for ciaB (encoding a putative invasion protein). Other putative virulence genes were present in other species, i.e., A. butzleri (cadF, cj1349, irgA, and hecA), A. trophiarum (cj1349), A. ellisii (cj1349), and A. defluvii (irgA). No virulence genes were detected in strains which showed little or no invasion of Caco-2 cells. These results indicate that many Arcobacter species are potential pathogens of humans and animals

A Low-Cost FPGA-Based Test and Diagnosis Architecture for SRAMs

The continues improvement of manufacturing technologies allows the realization of integrated circuits containing an ever increasing number of transistors. A major part of these devices is devoted to realize SRAM blocks. Test and diagnosis of SRAM circuits are therefore an important challenge for improving quality of next generation integrated circuits. This paper proposes a flexible platform for testing and diagnosis of SRAM circuits. The architecture is based on the use of a low cost FPGA based board allowing high diagnosability while keeping costs at a very low leve

Characterizing the Galactic warp with Gaia: I. The tilted ring model with a twist

We explore the possibility of detecting and characterizing the warp of the stellar disc of our Galaxy using synthetic Gaia data. The availability of proper motions and, for the brightest stars radial velocities, adds a new dimension to this study. A family of Great Circle Cell Counts (GC3) methods is used. They are ideally suited to find the tilt and twist of a collection of rings, which allow us to detect and measure the warp parameters. To test them, we use random realizations of test particles which evolve in a realistic Galactic potential warped adiabatically to various final configurations. In some cases a twist is introduced additionally. The Gaia selection function, its errors model and a realistic 3D extinction map are applied to mimic three tracer populations: OB, A and Red Clump stars. We show how the use of kinematics improves the accuracy in the recovery of the warp parameters. The OB stars are demonstrated to be the best tracers determining the tilt angle with accuracy better than $\sim0.5$ up to Galactocentric distance of $\sim16$ kpc. Using data with good astrometric quality, the same accuracy is obtained for A type stars up to $\sim13$ kpc and for Red Clump up to the expected stellar cut-off. Using OB stars the twist angle is recovered to within $< 3^\circ$ for all distances.Comment: 18 pages, 16 figures, accepted for publication in MNRA

Walkway on coastal dunes negatively affects mobility of the spiny–footed lizard Acanthodactylus erythrurus

Las pasarelas en las dunas costeras perjudican la movilidad de la lagartija colirroja Acanthodactylus erythrurus Los sistemas dunares son los ecosistemas más degradados de toda la costa europea y la actividad humana ha causado una profunda transformación en la costa mediterránea de España. En Torredembarra (Tarragona, España) habita una población de lagartija colirroja Acanthodactylus erythrurus en algunas de las dunas naturales y parches de vegetación que aún existen, donde la fauna silvestre coexiste con unas intensas actividades turísticas. Nuestro objetivo fue conocer si las pasarelas instaladas sobre las dunas estaban afectando a la movilidad de la lagartija colirroja. Se comparó la movilidad de lagartijas marcadas entre dos zonas con hábitat similar, pero una con pasarelas y otra sin. Se observó que las pasarelas acortaron las distancias entre reavistamientos consecutivos y que afectaban más a los juveniles de lagartija colirroja que a los adultos. Llegamos a la conclusión de que la pasarela puede estar afectando a las interacciones sociales naturales de la especie.Dune systems are the most degraded ecosystems of the entire European coast, and human activity on the Mediterranean coast of Spain has caused dramatic transformation. In Torredembarra (Tarragona, Spain), a population of spiny–footed lizards Acanthodactylus erythrurus inhabits the few remaining natural dunes and vegetation patches where wildlife coexists with intensive tourism activities. Our aim was to know whether walkways installed across the dunes were affecting the mobility of the spiny–footed lizard. We compared the mobility of marked lizards in two areas with a similar habitat, one with and one without a walkway. We found that the walkway reduced the distances between consecutive resightings, affecting juveniles more than adults. We conclude that the walkway may affect social interactions in the species.Las pasarelas en las dunas costeras perjudican la movilidad de la lagartija colirroja Acanthodactylus erythrurus Los sistemas dunares son los ecosistemas más degradados de toda la costa europea y la actividad humana ha causado una profunda transformación en la costa mediterránea de España. En Torredembarra (Tarragona, España) habita una población de lagartija colirroja Acanthodactylus erythrurus en algunas de las dunas naturales y parches de vegetación que aún existen, donde la fauna silvestre coexiste con unas intensas actividades turísticas. Nuestro objetivo fue conocer si las pasarelas instaladas sobre las dunas estaban afectando a la movilidad de la lagartija colirroja. Se comparó la movilidad de lagartijas marcadas entre dos zonas con hábitat similar, pero una con pasarelas y otra sin. Se observó que las pasarelas acortaron las distancias entre reavistamientos consecutivos y que afectaban más a los juveniles de lagartija colirroja que a los adultos. Llegamos a la conclusión de que la pasarela puede estar afectando a las interacciones sociales naturales de la especie

Monolithic integration of Giant Magnetoresistance (GMR) devices onto standard processed CMOS dies

Giant Magnetoresistance (GMR) based technology is nowadays the preferred option for low magnetic fields sensing in disciplines such as biotechnology or microelectronics. Their compatibility with standard CMOS processes is currently investigated as a key point for the development of novel applications, requiring compact electronic readout. In this paper, such compatibility has been experimentally studied with two particular non-dedicated CMOS standards: 0.35 μm from AMS (Austria MicroSystems) and 2.5 μm from CNM (Centre Nacional de Microelectrònica, Barcelona) as representative examples. GMR test devices have been designed and fabricated onto processed chips from both technologies. In order to evaluate so obtained devices, an extended characterization has been carried out including DC magnetic measurements and noise analysis. Moreover, a 2D-FEM (Finite Element Method) model, including the dependence of the GMR device resistance with the magnetic field, has been also developed and simulated. Its potential use as electric current sensors at the integrated circuit level has also been demonstrated