Superoxide reductase from Giardia intestinalis: structural characterization of the first sor from a eukaryotic organism shows an iron centre that is highly sensitive to photoreduction

Superoxide reductase (SOR), which is commonly found in prokaryotic organisms, affords protection from oxidative stress by reducing the superoxide anion to hydrogen peroxide. The reaction is catalyzed at the iron centre, which is highly conserved among the prokaryotic SORs structurally characterized to date. Reported here is the first structure of an SOR from a eukaryotic organism, the protozoan parasite Giardia intestinalis (GiSOR), which was solved at 2.0 Å resolution. By collecting several diffraction data sets at 100 K from the same flash-cooled protein crystal using synchrotron X-ray radiation, photoreduction of the iron centre was observed. Reduction was monitored using an online UV-visible microspectrophotometer, following the decay of the 647 nm absorption band characteristic of the iron site in the glutamate-bound, oxidized state. Similarly to other 1Fe-SORs structurally characterized to date, the enzyme displays a tetrameric quaternary-structure arrangement. As a distinctive feature, the N-terminal loop of the protein, containing the characteristic EKHxP motif, revealed an unusually high flexibility regardless of the iron redox state. At variance with previous evidence collected by X-ray crystallography and Fourier transform infrared spectroscopy of prokaryotic SORs, iron reduction did not lead to dissociation of glutamate from the catalytic metal or other structural changes; however, the glutamate ligand underwent X-ray-induced chemical changes, revealing high sensitivity of the GiSOR active site to X-ray radiation damage

Increased levels of interleukin-6 exacerbate the dystrophic phenotype in mdx mice

Duchenne muscular dystrophy (DMD) is characterized by progressive lethal muscle degeneration and chronic inflammatory response. The mdx mouse strain has served as the animal model for human DMD. However, while DMD patients undergo extensive necrosis, the affected muscles of adult mdx mice rapidly regenerates and regains structural and functional integrity. The basis for the mild effects observed in mice compared with the lethal consequences in humans remains unknown. In this study, we provide evidence that interleukin-6 (IL-6) is causally linked to the pathogenesis of muscular dystrophy. We report that forced expression of IL-6, in the adult mdx mice, recapitulates the severe phenotypic characteristics of DMD in humans. Increased levels of IL-6 exacerbate the dystrophic muscle phenotype, sustaining inflammatory response and repeated cycles of muscle degeneration and regeneration, leading to exhaustion of satellite cells. The mdx/IL6 mouse closely approximates the human disease and more faithfully recapitulates the disease progression in humans. This study promises to significantly advance our understanding of the pathogenic mechanisms that lead to DMD

A bag of tricks: Using proper motions of Galactic stars to identify the Hercules ultra-faint dwarf galaxy members

Hercules is the prototype of the ultra-faint dwarf (UFD) galaxies. To date, there are still no firm constraints on its total luminosity, due to the difficulty of disentangling Hercules bona-fide stars from the severe Galactic field contamination. In order to better constrain Hercules properties we aim at removing foreground and background contaminants in the galaxy field using the proper motions of the Milky Way stars and the colour-colour diagram. We have obtained images of Hercules in the rSloan, BBessel and Uspec bands with the Large Binocular Telescope (LBT) and LBC-BIN mode capabilities. The rSloan new data-set combined with data from the LBT archive span a time baseline of about 5 yr, allowing us to measure for the first time proper motions of stars in the Hercules direction. The Uspec data along with existing LBT photometry allowed us to use colour-colour diagram to further remove the field contamination. Thanks to a highly-accurate procedure to derive the rSloan-filter geometric distortion solution for the LBC-red, we were able to measure stellar relative proper motions to a precision of better than 5 mas yr^-1 down to rSloan=22 mag and disentangle a significant fraction (\>90\%) of Milky Way contaminants. We ended up with a sample of 528 sources distributed over a large portion of the galaxy body (0.12 deg^2). Of these sources, 171 turned out to be background galaxies and additional foreground stars, from the analysis of the Uspec - BBessel vs. BBessel - rSloan colour-colour diagram. This leaves us with a sample of 357 likely members of the Hercules UFD. We compared the cleaned colour-magnitude diagram (CMD) with evolutionary models and synthetic CMDs, confirming the presence in Hercules of an old population (t=12\pm 2 Gyr), with a wide spread in metallicity (-3.3\<[Fe/H]\<-1.8).Comment: 16 pages, 15 figures, 3 tables, accepted for publication in A&

Decentralized approach for translational motion estimation with multistatic inverse synthetic aperture radar systems

This paper addresses the estimation of the target translational motion by using a multistatic Inverse Synthetic Aperture Radar (ISAR) system composed of an active radar sensor and multiple receiving-only devices. Particularly, a two-step decentralized technique is derived: the first step estimates specific signal parameters (i.e., Doppler frequency and Doppler rate) at the single-sensor level, while the second step exploits these estimated parameters to derive the target velocity and acceleration components. Specifically, the second step is organized in two stages: the former is for velocity estimation, while the latter is devoted to velocity estimation refinement if a constant velocity model motion can be regarded as acceptable, or to acceleration estimation if a constant velocity assumption does not apply. A proper decision criterion to select between the two motion models is also provided. A closed-form theoretical performance analysis is provided for the overall technique, which is then used to assess the achievable performance under different distributions of the radar sensors. Additionally, a comparison with a state-of-the-art centralized approach has been carried out considering computational burden and robustness. Finally, results obtained against experimental multisensory data are shown confirming the effectiveness of the proposed technique and supporting its practical application

Musculoskeletal disorders among robotic surgeons: A questionnaire analysis

Objective: Robotic surgical systems offer better workplace in order to relieve surgeons from prolonged physical efforts and improve their surgical outcomes. However, robotic surgery could produce musculoskeletal disorders due to the prolonged sitting position of the operator, the fixed position of the console viewer and the movements of the limbs. Until today, no one study has been reported concerning the association between robotics and musculoskeletal pain. The aim of this work was verify the prevalence of musculoskeletal disorders among Italian robotic surgeons. Material and methods: Between July 2011 and April 2012 a modified Standardized Nordic Questionnaire was delivered to thirty-nine Italian robotic centres. Twentytwo surgeons (56%) returned the questionnaires but only seventeen questionnaires (43.5%) were evaluable. Results: Seven surgeons (41.2%) reported musculoskeletal disorders, by since their first use of the robot which significantly persisted during the daily surgical activity (P &lt; 0.001). Regarding the body parts affected, musculoskeletal disorders were mainly reported in the cervical spine (29.4%) and in the upper limbs (23.5%). Six surgeons (35.3%) defined the robotic console as less comfortable or neither comfortable/uncomfortable with a negative influence on their surgical procedures. Conclusions: In spite of some important limitations, our data showed musculoskeletal disorders due to posture discomfort with negative impact on daily surgical activity among robotic surgeons. These aspects could be due to the lack of ergonomic seat and to the fixed position of the console viewer which could have produced an inadequate spinal posture. The evaluation of these postural factors, in particular the development of an integrated and more ergonomic chair, could further improve the comfort feeling of the surgeon at the console and probably his surgical outcomes

Hide and seek between Andromeda's halo, disk, and giant stream

Photometry in B, V (down to V ~ 26 mag) is presented for two 23' x 23' fields of the Andromeda galaxy (M31) that were observed with the blue channel camera of the Large Binocular Telescope during the Science Demonstration Time. Each field covers an area of about 5.1kpc x 5.1kpc at the distance of M31 ((m-M)o ~ 24.4 mag), sampling, respectively, a northeast region close to the M31 giant stream (field S2), and an eastern portion of the halo in the direction of the galaxy minor axis (field H1). The stream field spans a region that includes Andromeda's disk and the giant stream, and this is reflected in the complexity of the color magnitude diagram of the field. One corner of the halo field also includes a portion of the giant stream. Even though these demonstration time data were obtained under non-optimal observing conditions the B photometry, acquired in time-series mode, allowed us to identify 274 variable stars (among which 96 are bona fide and 31 are candidate RR Lyrae stars, 71 are Cepheids, and 16 are binary systems) by applying the image subtraction technique to selected portions of the observed fields. Differential flux light curves were obtained for the vast majority of these variables. Our sample includes mainly pulsating stars which populate the instability strip from the Classical Cepheids down to the RR Lyrae stars, thus tracing the different stellar generations in these regions of M31 down to the horizontal branch of the oldest (t ~ 10 Gyr) component.Comment: 59 pages, 26 figures, 12 tables, ApJ in pres

A Case Study of Multidisciplinary Surface Faulting Assessment in the Urbanized Fucino Basin, Italy

The occurrence of coseismic surface ruptures along fault traces in urbanised areas creates a serious hazard to the vulnerability of man-made manufactures. In order to mitigate such hazard, it is necessary to investigate the geometry, the activity and the capability of faults located close to urbanised areas. This paper presents a case study of the investigation of capable faults within a sensitive area in Italy that is characterized by a high density of population and industrial activities, high levels of seismicity and the presence of faults proven to be capable of rupturing the surface during medium-to-large earthquakes. We focused on the Luco fault (Fucino basin, Central Italy), which previous studies have suggested to cross the industrial district of the town of Avezzano. We present a multidisciplinary approach, consisting of Electrical Resistivity Tomography surveys, continuous-coring boreholes and paleoseismological trenches, aimed at accurately constraining the trace of the Luco fault and documenting the associated fault displacement. This allowed us to constrain the geometry of the Luco fault and to assess the associated fault displacement hazard. We suggest that the proposed methodology represents a pilot study for further investigations of capable faults in the Italian and other similar seismotectonic contexts.Published104-121OST3 Vicino alla fagliaJCR Journa

Integration of new biological and physical retrospective dosimetry methods into EU emergency response plans : joint RENEB and EURADOS inter-laboratory comparisons

Purpose: RENEB, 'Realising the European Network of Biodosimetry and Physical Retrospective Dosimetry,' is a network for research and emergency response mutual assistance in biodosimetry within the EU. Within this extremely active network, a number of new dosimetry methods have recently been proposed or developed. There is a requirement to test and/or validate these candidate techniques and inter-comparison exercises are a well-established method for such validation. Materials and methods: The authors present details of inter-comparisons of four such new methods: dicentric chromosome analysis including telomere and centromere staining; the gene expression assay carried out in whole blood; Raman spectroscopy on blood lymphocytes, and detection of radiation induced thermoluminescent signals in glass screens taken from mobile phones. Results: In general the results show good agreement between the laboratories and methods within the expected levels of uncertainty, and thus demonstrate that there is a lot of potential for each of the candidate techniques. Conclusions: Further work is required before the new methods can be included within the suite of reliable dosimetry methods for use by RENEB partners and others in routine and emergency response scenarios

Reinforcement of Polylactic Acid / Poly Butylene Adipate-co-Terephthalate blends by starch addition: a coupled computational and experimental study

Poly Lactic Acid / Poly Butylene Adipate-co-Terephthalate blends are used as packaging green materials since they constitute hydrophilic and biodegradable plastic. With the aim of improving the mechanical characteristics of such blends as biodegradable packaging materials for food products the addition of starch has been considered. In silico test performed by classical molecular dynamics highlighted that the addition of starch can reinforce the polymeric structure via starch-polymer interactions, suggesting that starch can be a suitable material to be added to the Poly Lactic Acid / Poly Butylene Adipate-co-Terephthalate blend to obtain more resistant packaging materials. Experimental analysis of the mechanical properties of the polymeric blend containing different amounts of starch confirmed what foreseen by MD, highlighting an increase of Young modulus and glass transition as a function of added starch. The coupled theoretical/experimental approach constitutes added value of the present work, furnishing important data on the reinforcement of the packaging material performances and a molecule-based interpretation and comprehension of the observed phenomenon