Methylglyoxal-dependent glycative stress and deregulation of SIRT1 functional network in the ovary of PCOS mice

Advanced glycation end-products (AGEs) are involved in the pathogenesis and consequences of polycystic ovary syndrome (PCOS), a complex metabolic disorder associated with female infertility. The most powerful AGE precursor is methylglyoxal (MG), a byproduct of glycolysis, that is detoxified by the glyoxalase system. By using a PCOS mouse model induced by administration of dehydroepiandrosterone (DHEA), we investigated whether MG-dependent glycative stress contributes to ovarian PCOS phenotype and explored changes in the Sirtuin 1 (SIRT1) functional network regulating mitochondrial functions and cell survival. In addition to anovulation and reduced oocyte quality, DHEA ovaries revealed altered collagen deposition, increased vascularization, lipid droplets accumulation and altered steroidogenesis. Here we observed increased intraovarian MG-AGE levels in association with enhanced expression of receptor for AGEs (RAGEs) and deregulation of the glyoxalase system, hallmarks of glycative stress. Moreover, DHEA mice exhibited enhanced ovarian expression of SIRT1 along with increased protein levels of SIRT3 and superoxide dismutase 2 (SOD2), and decreased peroxisome proliferator-activated receptor gamma co-activator 1 alpha (PGC1 alpha), mitochondrial transcriptional factor A (mtTFA) and translocase of outer mitochondrial membrane 20 (TOMM20). Finally, the presence of autophagy protein markers and increased AMP-activated protein kinase (AMPK) suggested the involvement of SIRT1/AMPK axis in autophagy activation. Overall, present findings demonstrate that MG-dependent glycative stress is involved in ovarian dysfunctions associated to PCOS and support the hypothesis of a SIRT1-dependent adaptive response

Measurements and calibration of the stripline BPM for the ELI-NP facility with the stretched wire method

A methodology has been developed to perform electrical characterization of the stripline BPMs for the future Gamma Beam System of ELI Nuclear Physics facility in Romania. Several prototype units are extensively benchmarked and the results are presented in this paper. The BPM sensitivity function is determined using a uniquely designed motorized test bench with a stretched wire to measure the BPM response map. Here, the BPM feedthroughs are connected to Libera Brilliance electronics and the wire is fed by continuous wave signal, while the two software-controlled motors provide horizontal and vertical motion of the BPM around the wire. The electrical offset is obtained using S-parameter measurements with a Network Analyzer (via the “Lambertson” method) and is referenced to the mechanical offse

Preliminary assessment of a two-phase direct cooling of Lithium-Ion battery pack for e-bike mobility

Electric mobility is playing an increasingly central role in emission reduction policies to mitigate climate change effect. During the operation of electric vehicles, the batteries may be subject to high variation of the required current, which can lead to a sudden increase in the cell temperature. If this condition occurs repeatedly, there would be a reduction in battery capacity and useful life, and autonomy reduction of the electric vehicle. In the worst case, this problem can lead to the thermal runaway. Therefore, cooling of electric vehicle propulsion systems is a fundamental issue for the electric mobility development. In this article we propose an innovative cooling system using a dielectric low boiling fluid in which the batteries are directly immersed. The system was tested on an electric bicycle under real operative conditions. A special test bench was realized, consisting of a real electric bicycle, a training roller to simulate the load due to road slope and an external electric motor to simulate the pedaling of the cyclist. The results show a substantial decrease in the temperature of the cells with the proposed cooling system and there was a marked improvement in the temperature uniformity of the cells inside the battery pack

Assessment of a Flow-dependent Subgrid Characteristic Length for Large-Eddy Simulation on Anisotropic Grids

This paper presents the latest results of a long track development activity in the context of low-dissipative finite volume method for compressible flows. Specifically, here we focus our attention on the Large-Eddy Simulation (LES) approach which can be considered a good candidate for turbulent flow simulations over the next decades. One of the key ingredients of LES models is the subgrid length scale which is typically evaluated based on the local mesh size. This standard approach suffers from loss of accuracy on anisotropic grids that are commonly employed to obtain sufficient wall-normal resolution, whilst keeping the total cell count to a minimum. In order to avoid this issue, we assess the effectiveness of a velocity-gradient-based length scale, referred to as least square length (LSQ) [1]. In this paper, we present for the first time results obtained with the LSQ length scale in the context of compressible LES. The superiority of the LSQ approach over the standard cubic-root length scale is demonstrated in terms of accuracy and overall time to solution

Development of a Renewable Energy Forecasting Strategy Based on Numerical Weather Prediction for the Cold Ironing System at the Port of Ancona, Italy

Received: 15 October 2024. Revised: 14 November 2024. Accepted: 30 November 2024. Available online: 31 December 2024.Since renewable energy sources have an intermittent nature, forecasting strategies are increasingly important. In parallel, ports are characterized by large energy demands, especially from berthed ships. Cold ironing systems have already been proven to reduce their environmental impact by connecting ships to the electricity grid and allowing them to switch off their auxiliary engines in port. In this work, a local energy production, consisting of photovoltaic, wind turbines, and an energy storage system, is proposed to cover the energy demand of ships. In addition, an energy forecasting strategy is presented, where the solar and wind energy potential is provided by the Weather and Research Forecasting (WRF) mesoscale model. By forecasting the energy production for the following day, the storage system can be charged from the grid at night, namely in offpeak periods, reducing the pressure on the grid in on-peak periods. The methodology is tested on the port of Ancona (Italy). Results show that energy production can directly cover 54% of energy demand, and up to 70% by adding the storage system. The forecasting strategy reduces the energy withdrawn during the daytime by 24.9% and increases that during the nighttime by 18.9%, proving the effectiveness of the proposed strategy

High power test results of the Eli-NP S-Band gun fabricated with the new clamping technology without brazing

High gradient RF photoguns have been a key development to enable several applications of high quality electron beams. They allow the generation of beams with very high peak current and low transverse emittance, thus satisfying the tight demands of free-electron lasers, energy recovery linacs, Compton/Thomson sources and high-energy linear colliders. A new fabrication technique for this type of structures has been recently developed and implemented at the Laboratories of Frascati of the National Institute of Nuclear Physics (INFN-LNF, Italy). It is based on the use of special RF-vacuum gaskets, that allow a brazing-free realization process. The S-band gun of the ELI-NP gamma beam system (GBS) has been fabricated with this new technique. It operates at 100 Hz with 120 MV/m cathode peak field and 1.5 μs long RF pulses to house the 32 bunches necessary to reach the target gamma flux. High gradient tests, performed at full power and full repetition rate, have shown extremely good performances of the structure in terms of breakdown rate. In the paper, we report and discuss all the experimental results, the electromagnetic design and the mechanical realization processes

Endothelial cells from umbilical cord of women affected by gestational diabetes: A suitable in vitro model to study mechanisms of early vascular senescence in diabetes

Human umbilical cord endothelial cells (HUVECs) obtained from women affected by gestational diabetes (GD-HUVECs) display durable pro-atherogenic modifications and might be considered a valid in vitro model for studying chronic hyperglycemia effects on early endothelial senescence. Here, we demonstrated that GD- compared to C-HUVECs (controls) exhibited oxidative stress, altered both mitochondrial membrane potential and antioxidant response, significant increase of senescent cells characterized by a reduced NAD-dependent deacetylase sirtuin-1 (SIRT1) activity together with an increase in cyclin-dependent kinase inhibitor-2A (P16), cyclin-dependent kinase inhibitor-1 (P21), and tumor protein p53 (P53) acetylation. This was associated with the p300 activation, and its silencing significantly reduced the GD-HUVECs increased protein levels of P300 and Ac-P53 thus indicating a persistent endothelial senescence via SIRT1/P300/P53/P21 pathway. Overall, our data suggest that GD-HUVECs can represent an “endothelial hyperglycemic memory” model to investigate in vitro the early endothelium senescence in cells chronically exposed to hyperglycemia in vivo

TEX (TEst stand for X-band) at LNF

TEX facility if commissioned for high power testing to characterize accelerating structures and validate them for the operation on future particle accelerators for medical, industrial and research applications. At this aim, TEX is directly involved in the LNF leading project EuPRAXIA@SPARC_Lab. The brief description of the facility and its status and prospective will be provided.Comment: Talk presented at the International Workshop on Future Linear Colliders (LCWS 2023), 15-19 May 2023. C23-05-15.