Black-hole masses of type 1 AGN in the XMM-Newton bright serendipitous survey

We derive masses of the central super-massive black hole (SMBH) and accretion rates for 154 type1 AGN belonging to a well-defined X-ray-selected sample, the XMM-Newton Serendipitous Sample (XBS). To this end, we use the most recent "single-epoch" relations, based on Hbeta and MgII2798A emission lines, to derive the SMBH masses. We then use the bolometric luminosities, computed on the basis of an SED-fitting procedure, to calculate the accretion rates, both absolute and normalized to the Eddington luminosity (Eddington ratio). The selected AGNs cover a range of masses from 10^7 to 10^10 Msun with a peak around 8x10^8 Msun and a range of accretion rates from 0.01 to ~50 Msun/year (assuming an efficiency of 0.1), with a peak at ~1 Msun/year. The values of Eddington ratio range from 0.001 to ~0.5 and peak at 0.1.Comment: 14 pages, 11 figures. Accepted for publication in Astronomy & Astrophysic

Photografted methacrylate-based monolithic columns coated with cellulose tris(3,5-dimethylphenylcarbamate) for chiral separation in CEC

A chiral capillary monolithic column for enantiomer separation in capillary electrochromatography was prepared by coating cellulose tris(3,5-dimethylphenylcarbamate) on porous glycidyl methacrylate-co-ethylene dimethacrylate monolith in capillary format grafted with chains of [2(methacryloyloxy)ethyl] trimethylammonium chloride. The surface modification of the monolith by the photografting of [2(methacryloyloxy)ethyl] trimethylammonium chloride monomer as well as the coating conditions of cellulose tris(3,5-dimethylphenylcarbamate) onto the grafted monolithic scaffold were optimized to obtain a stable and reproducible chiral stationary phase for capillary electrochromatography. The effect of organic modifier (acetonitrile) in aqueous mobile phase for the enantiomer separation by capillary electrochromatography was also investigated. Several pairs of enantiomers including acidic, neutral, and basic analytes were tested and most of them were partially or completely resolved under aqueous mobile phases. The prepared monolithic chiral stationary phases exhibited a good stability, repeatability, and column-to-column reproducibility, with relative standard deviations below 11% in the studied electrochromatographic parameters.Fil: Echevarria, Romina Noel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; ArgentinaFil: Carrasco Correa, Enrique Javier. Universidad de Valencia; EspañaFil: Keunchkarian, Sonia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; ArgentinaFil: Reta, Mario Roberto. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Herrero Martinez, José Manuel. Universidad de Valencia; Españ

SDSSJ143244.91+301435.3: a link between radio-loud narrow-line Seyfert 1 galaxies and compact steep-spectrum radio sources?

We present SDSSJ143244.91+301435.3, a new case of radio-loud narrow line Seyfert 1 (RL NLS1) with a relatively high radio power (P1.4GHz=2.1x10^25 W Hz^-1) and large radioloudness parameter (R1.4=600+/-100). The radio source is compact with a linear size below ~1.4 kpc but, contrary to most of the RL NLS1 discovered so far with such a high R1.4, its radio spectrum is very steep (alpha=0.93) and not supporting a 'blazar-like' nature. Both the small mass of the central super-massive black-hole and the high accretion rate relative to the Eddington limit estimated for this object (3.2x10^7 Msun and 0.27, respectively, with a formal error of ~0.4 dex on both quantities) are typical of the class of NLS1. Through a modeling of the spectral energy distribution of the source we have found that the galaxy hosting SDSSJ143244.91+301435.3 is undergoing a quite intense star-formation (SFR=50 Msun y^-1) which, however, is expected to contribute only marginally (~1 per cent) to the observed radio emission. The radio properties of SDSSJ143244.91+301435.3 are remarkably similar to those of compact steep spectrum (CSS) radio sources, a class of AGN mostly composed by young radio galaxies. This may suggest a direct link between these two classes of AGN, with the CSS sources possibly representing the misaligned version (the so-called parent population) of RL NLS1 showing blazar characteristics.Comment: 14 pages, 7 figures, 4 tables, accepted for publication in MNRA

Reversible two-step unfolding of heme-human serum albumin: A 1H-NMR relaxometric and circular dichroism study

Human serum albumin (HSA) participates in heme scavenging, the bound heme turning out to be a reactivity center and a powerful spectroscopic probe. Here, the reversible unfolding of heme-HSA has been investigated by H-1-NMR relaxometry, circular dichroism, and absorption spectroscopy. In the presence of 6 equiv of myristate ( thus fully saturating all available fatty acid binding sites in serum heme-albumin), 1.0 M guanidinium chloride induces some unfolding of heme-HSA, leading to the formation of a folding intermediate; this species is characterized by increased relaxivity and enhanced dichroism signal in the Soret region, suggesting a more compact heme pocket conformation. Heme binds to the folding intermediate with K-d = (1.2 +/- 0.1) x 10(-6) M. In the absence of myristate, the conformation of the folding intermediate state is destabilized and heme binding is weakened [K-d = (3.4 +/- 0.1) x 10(-5) M]. Further addition of guanidinium chloride ( up to 5 M) brings about the usual denaturation process. In conclusion, myristate protects HSA from unfolding, stabilizing a folding intermediate state in equilibrium with the native and the fully unfolded protein, envisaging a two-step unfolding pathway for heme-HSA in the presence of myristate

Interaction of anticancer reduced Schiff base coumarin derivatives with human serum albumin investigated by fluorescence quenching and molecular modeling

The specific binding of five reduced Schiff base derived 7-amino-coumarin compounds with antitumor activity to human serum albumin, the principal binding protein of blood, was studied by fluorescence spectroscopy. Their conditional binding constants were computed and the reversible binding at the Sudlow’s site I was found to be strong (KD ~ 0.03-2.09 M). Based on the data albumin can provide a depot for the compounds and is responsible for their biodistribution and transport processes. The experimental data is complemented by protein– ligand docking calculations for two representatives which support the observations. The proton dissociation constants of the compounds were also determined by UV-Vis spectrophotometric and fluorometric titrations to obtain the actual charges and distribution of the species in the various protonation states at physiological pH

An enantioselective high-performance liquid chromatography-mass spectrometry method to study the fate of quizalofop-P-ethyl in soil and selected agricultural products

In this study, the attention was focused on quizalofop-ethyl, a chiral herbicide whose formulation has recently been marketed as quizalofop-P-ethyl, i.e. the (+)-enantiomer exhibiting herbicidal activity. To verify the real enantiomeric purity of this product as well as to study its environmental fate, the enantioselective separation of the P- and M- enantiomers of quizalofop-ethyl was achieved on Lux Cellulose-2 column (3-chloro,4-methylphenilcarbamate cellulose) under isocratic conditions in polar organic mode. Once established that the commercial formulation contains ̃0.6% (enantiomeric fraction) of M as an impurity, an HPLC-MS/MS method was developed, validated and applied to the analysis of soil, carrots and turnips treated with the herbicide. A simple solid-liquid extraction allowed recoveries greater than 70%; limits of detections of P and M enantiomers were below 5 ng/g. The analyses of the real samples showed a modification of the enantiomeric fraction of quizalofop-M-ethyl between the commercial formulation (EFM = 0.63 & PLUSMN; 0.03%) and the analysed matrices (EFM = 7.6 & PLUSMN; 0.1% for carrots; EFM = 0% for the other matrices). This outcome highlighted the occurrence of an enantioselective biotic dissipation, responsible for a greater persistency of the distomer in carrots. On the other hand, since screening analyses revealed the occurrence of residues of the metabolite quizalofop-acid with the same EFs as the ester precursor, it was concluded that the hydrolytic conversion was an abiotic process