Bacillus sp. alone or combined with salicylic acid inhibited Trichoderma spp. infection on harvested white Hypsizygus marmoreus

IntroductionWhite Hypsizygus marmoreus is a popular edible mushroom. It is rich in nutrition and flavor but vulnerable to fungal disease, resulting in nutrient loss and aging.MethodsIn this study, the pathogenic fungus Trichoderma spp. BBP-6 and its antagonist Bacillus sp. 1–23 were isolated and identified. The negative effects caused by this pathogen were judged by detecting a series of changes in the infected white H. marmoreus. The effects of Bacillus sp. 1–23 on Trichoderma spp. BBP-6 and the infected white H. marmoreus were detected. The effect of Bacillus sp. 1–23 treatment combined with salicylic acid (SA) was also considered.ResultsThe results showed that Trichoderma spp. BBP-6 could affect the activities of antioxidant enzymes PAL, POD, CAT, SOD, GR, PPO, and APX to interfere with the stability of the white H. marmoreus antioxidant enzyme system and cause the mushroom severe browning and nutrition loss, as well as general quality deterioration. Bacillus sp. 1–23 could produce chitinase and chitosanase enzymes to inhibit Trichoderma spp. BBP-6 directly. SA reinforced this inhibitory. Bacillus sp. 1–23 alone or combined with SA could help white H. marmoreus from the Trichoderma spp. BBP-6 infection to effectively maintain nutrients, restore and stabilize the antioxidant system, and reduce the production of malondialdehyde, superoxide anion and hydrogen peroxide.DiscussionThus, such treatments could be considered potential methods to alleviate damage from disease and extend the shelf life of white H. marmoreus

The potential linkage between sediment oxygen demand and microbes and its contribution to the dissolved oxygen depletion in the Gan River

The role of sediment oxygen demand (SOD) in causing dissolved oxygen (DO) depletion is widely acknowledged, with previous studies mainly focusing on chemical and biological SOD separately. However, the relationship between the putative functions of sediment microbes and SOD, and their impact on DO depletion in overlying water, remains unclear. In this study, DO depletion was observed in the downstream of the Gan River during the summer. Sediments were sampled from three downstream sites (YZ, Down1, and Down2) and one upstream site (CK) as a control. Aquatic physicochemical parameters and SOD levels were measured, and microbial functions were inferred from taxonomic genes through analyses of the 16S rRNA gene. The results showed that DO depletion sites exhibited a higher SOD rate compared to CK. The microbial community structure was influenced by the spatial variation of Proteobacteria, Chloroflexi, and Bacteroidota, with total organic carbon (TOC) content acting as a significant environmental driver. A negative correlation was observed between microbial diversity and DO concentration (p < 0.05). Aerobic microbes were more abundant in DO depletion sites, particularly Proteobacteria. Microbes involved in various biogeochemical cycles, such as carbon (methane oxidation, methanotrophs, and methylotrophs), nitrogen (nitrification and denitrification), sulfur (sulfide and sulfur compound oxidation), and manganese cycles (manganese oxidation), exhibited higher abundance in DO depletion sites, except for the iron cycle (iron oxidation). These processes were negatively correlated with DO concentration and positively with SOD (p < 0.05). Overall, the results highlight that aerobic bacteria’s metabolic processes consume oxygen, increasing the SOD rate and contributing to DO depletion in the overlying water. Additionally, the study underscores the importance of targeting the removal of in situ microbial molecular mechanisms associated with toxic H2S and CH4 to support reoxygenation efforts in rehabilitating DO depletion sites in the Gan River, aiding in identifying factors controlling DO consumption and offering practical value for the river’s restoration and management

Effects of Pretreatment with Three Different Enzymes on Physicochemical Properties, Biological Activity andSensory of Banana Jiaosu

In order to compare the effects of different enzyme pretreatments on the quality of fruit fermented Jiaosu. Bananas were used as raw materials in this paper. Cellulase, pectinase and papain were used to pretreat bananas, and the effects of different enzyme pretreatments on the physical and chemical indexes, antioxidant, sensory of banana enzyme were compared. The results showed that enzyme treatment had little effect on pH and total acid. The ability of reducing sugar utilization in the enzyme pretreatment group was significant, and the final sugar content was reduced by about 10 g/L. At 72 h of fermentation, the total phenolic content of cellulase and papain treatment groups was significantly increased by about 1.31 times (P<0.05), and the flavonoid content of cellulase treatment group was significantly increased by about 40% compared with the other treatment groups (P<0.05). After 72 h fermentation, the SOD activity of cellulase treatment group was significantly increased by about 9 times compared with other groups, and the other two groups were significantly increased by about 3 times (P<0.05). During the fermentation process, the ability of cellulase pretreatment group to produce γ-aminobutyric acid was significantly and increased by 25% at 72 h (P<0.05). The pretreatment of cellulase, papain and pectinase increased the DPPH and ABTS+ free radical scavenging activity and FRAP reducing power during the fermentation of banana enzyme, and enhanced the antioxidant activity. Compared with other enzymes, cellulase was more powerful in the utilization of fermented sugar and the ability to produce SOD enzymes, and had high antioxidant activity and excellent taste. This study provides a theoretical basis for the production of banana Jiaosu, and expands the research and application of banana Jiaosu

Wafer-scale arrayed p-n junctions based on few-layer epitaxial GaTe

Two-dimensional (2D) materials have attracted substantial attention in electronic and optoelectronic applications with superior advantages of being flexible, transparent and highly tunable. Gapless graphene exhibits ultra-broadband and fast photoresponse while the 2D semiconducting MoS2 and GaTe unveil high sensitivity and tunable responsivity to visible light. However, the device yield and the repeatability call for a further improvement of the 2D materials to render large-scale uniformity. Here we report a layer-by-layer growth of wafer-scale GaTe with a hole mobility of 28.4 cm2/Vs by molecular beam epitaxy. The arrayed p-n junctions were developed by growing few-layer GaTe directly on three-inch Si wafers. The resultant diodes reveal good rectifying characteristics, photoresponse with a maximum photoresponsivity of 2.74 A/W and a high photovoltaic external quantum efficiency up to 62%. The photocurrent reaches saturation fast enough to capture a time constant of 22 {\mu}s and shows no sign of device degradation after 1.37 million cycles of operation. Most strikingly, such high performance has been achieved across the entire wafer, making the volume production of devices accessible. Finally, several photo-images were acquired by the GaTe/Si photodiodes with a reasonable contrast and spatial resolution, demonstrating for the first time the potential of integrating the 2D materials with the silicon technology for novel optoelectronic devices

Mantle Flow Underneath the South China Sea Revealed by Seismic Anisotropy

It Has Long Been Established that Plastic Flow in the Asthenosphere Interacts Constantly with the overlying Lithosphere and Plays a Pivotal Role in Controlling the Occurrence of Geohazards Such as Earthquakes and Volcanic Eruptions. Unfortunately, Accurately Characterizing the Direction and Lateral Extents of the Mantle Flow Field is Notoriously Difficult, Especially in Oceanic Areas Where Deployment of Ocean Bottom Seismometers (OBSs) is Expensive and Thus Rare. in This Study, by Applying Shear Wave Splitting Analyses to a Dataset Recorded by an OBS Array that We Deployed between Mid-2019 and Mid-2020 in the South China Sea (SCS), We Show that the Dominant Mantle Flow Field Has a NNW-SSE Orientation, Which Can Be Attributed to Mantle Flow Extruded from the Tibetan Plateau by the Ongoing Indian-Eurasian Collision. in Addition, the Results Suggest that E-W Oriented Flow Fields Observed in South China and the Indochina Peninsula Do Not Extend to the Central SCS

Fuzzy control and simulation of boiler drum water level

Boiler drum water level control system is hard to develop practical mathematical model and has nonlinear, stable and delay characteristics. The traditional boiler drum water level control often uses three impulse PID control method. The existing problem of ordinary PID control strategy is: once good parameter setting, it is hard to adapt to the change of the operation of the system dynamic greatly. In order to solve the problems of the above, the research object of the subject is a certain 120 t/h boiler steam capacity, the boiler drum water level control system is designed, and the control strategies are studied. In order to obtain better contrast, the ordinary PID controller and Fuzzy controller are designed, and the control strategies of the simulation are analyzed