2009-2010 Nothing but Klezmer

Concert Dates: Friday, March 19, 2010 at 7:30 pm Saturday, March 20, 2010 at 7:30 pmhttps://spiral.lynn.edu/conservatory_otherseasonalconcerts/1027/thumbnail.jp

2007-2008 Nothing but Klezmer

Concert Dates: Friday, March 21, 2008 at 7:30 pm Saturday, March 22, 2008 at 7:30 pmhttps://spiral.lynn.edu/conservatory_otherseasonalconcerts/1034/thumbnail.jp

2008-2009 Nothing but Klezmer

Concert Dates: Friday, December 12, 2008 at 7:30 pm Saturday, December 13, 2008 at 4:00 pm Saturday, December 13, 2008 at 7:30 pmhttps://spiral.lynn.edu/conservatory_otherseasonalconcerts/1032/thumbnail.jp

2010-2010 Nothing but Klezmer

https://spiral.lynn.edu/conservatory_otherseasonalconcerts/1024/thumbnail.jp

2011-2012 Nothing but Klezmer

https://spiral.lynn.edu/conservatory_otherseasonalconcerts/1023/thumbnail.jp

Real time micro-fiberoptic monitoring of endogenous fluorescence in the rat conceptus during hypoxia

A micro-fiberoptic methodology has been developed for noninvasive, real time measurement of endogenous pyridine nucleotide fluorescence from the surface of the visceral yolk sac (VYS) in intact, viable rat conceptuses. Gestational day (GD) 10–12 conceptuses are maintained in a customized perifusion system, which allows for control of oxygenation, as well as the continuous measurement of pH and oxygen concentration in the effluent perifusate. Miniaturized light guides were constructed by drawing 250 Μm ESKA acrylic optical fibers through a stainless steel sheath with a high strength epoxy polymer. A single fiber supplied the excitation signal from a mercury arc lamp at a wavelength of 366 nm. The emission signal was returned via three additional fibers, electronically amplified, processed, and recorded, using a dual channel lamp-compensated fluorometer, optimized for detection of reduced pyridine nucleotides at 455 nm. Endogenous fluorescence in the conceptus was monitored by placing the polished tip of the sensor directly on the surface of the VYS. Oxygen-equilibrated conceptuses, exposed to 100% nitrogen, produced a reproducible biphasic surface fluorescence peak, which returned to baseline levels upon reoxygenation of the perifusate. This biphasic response consisted of an initial rapid rise in fluorescence (phase I), followed by an attenuated rate in fluorescence signal increase (phase II). The hypoxia produced age-dependent rates of fluorescence change during phase I, while phase II remained relatively unchanged throughout GD 10–12. These results demonstrate the ability to monitor endogenous fluorescence, non-invasively and in real time, during the period of organogenesis in the intact rat conceptus and will provide valuable information in studies of embryonic metabolism and response to chemical embryotoxicants. © 1993 Wiley-Liss, Inc.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/38167/1/1420480408_ftp.pd

Cerebral ornithine decarboxylase levels following gestational exposure to cocaine

The pre- and postnatal developmental course of cerebral ornithine decarboxylase (ODC) has been studied in infant rats after treatment of pregnant dams with cocaine. Levels of cocaine attained in brains and serum of embryos were not initially increased over corresponding maternal values, but were more persistent. However, cocaine was not longer detectable in these tissues 4 days after administration. The cerebral ODC level of treated pups was initially depressed and subsequently elevated relative to control values. These changes were apparent at times when cocaine was not detected in the developing brain. Results indicate that a transient exposure to cocaine in utero may lead to prolonged developmental abnormality

Methanol, formaldehyde, and sodium formate exposure in rat and mouse conceptuses: A potential role of the visceral yolk sac in embryotoxicity

BACKGROUND Methanol (CH 3 OH) is believed to be teratogenic based on rodent studies. The mouse is more sensitive than the rat, but mechanisms of toxicity and identification of teratogenic metabolites are uncertain. METHODS Rat and mouse whole embryo cultures are used to distinguish toxicity of CH 3 OH and its metabolites, formaldehyde (HCHO) and formate (HCOONa), which are produced following transit through the visceral yolk sac (VYS), via addition to culture medium, or by direct embryonic exposure through microinjection into the amnion. RESULTS Embryonic viability, increased dysmorphogenesis, and decreased growth parameters were altered in a dose-dependent fashion for each compound. Mouse embryos were more sensitive than rat, as indicated by significant decreases in viability at comparable, lower concentrations. HCHO produced dysmorphogenesis and caused embryolethality at nearly 1000-fold lower concentrations (0.004 mg/ml) than seen with either CH 3 OH or HCOONa. All agents produced incomplete axial rotation and delayed neural tube closure in mice, but only CH 3 OH elicited similar effects in the rat. Increased growth retardation, blood pooling in the head and VYS, enlarged pericardium, accumulation of necrotic matter in the amnion, and hypoplastic prosencephalon were observed in both species with all compounds. Microinjection of compounds into the amnion produced higher mortality in mouse and rat, compared to equimolar amounts added to the culture medium. CH 3 OH did not prevent neural tube closure in the rat when microinjected. CONCLUSIONS HCHO is the most embryotoxic CH 3 OH metabolite and elicits the entire spectrum of lesions produced by CH 3 OH. The VYS serves a general protective role against toxicity and inherent differences in the embryonic metabolism of CH 3 OH may determine species sensitivity. Birth Defects Research (Part A), 2004. © 2004 Wiley-Liss, Inc.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/35296/1/20094_ftp.pd