Circ-0000979 promotes the development of gastric carcinoma by sponging miR-136 and modulating SP1 mRNA expression level

Circular RNAs (circRNAs) are a new class of non-coding RNAs that play pivotal biological roles in several types of cancer cells. However, the role of circ0000979 in gastric cancer (GC) has never been explored. Therefore, the current study aims to examine the functional effects of circ-0000979 in GC development and progression. The expression level of circ-0000979 was validated using qRT-PCR analysis. We found that circ-0000979 is significantly upregulated in GC samples. Using AGS and HGC27 GC cell line, we examined the biological functions and regulatory mechanisms of circ0000979 in GC in vitro and in vivo by knocking down circ-0000979. We found that circ-0000979 is subcellularly localized in the cytoplasm of GC cells. Functionally, silencing circ-0000979 leads to a significant reduction in GC cell proliferation and migration. In vivo assays showed that circ-0000979 knockdown markedly reduced GC tumor growth. CircRNA interactome predicted miR-136 as circ0000979 targeting miRNA, while starbase prediction result showed that miR-136 targeted the 3’UTR region of SP1 mRNA. Taken together, our results demonstrated that circ-0000979, as a carcinogenic circRNA, promotes the progression of GC by regulating the miR-136/SP1 pathway. Circ-0000979 is a potential RNA-based therapeutic target for GC treatment

An ancestral NB-LRR with duplicated 3'UTRs confers stripe rust resistance in wheat and barley.

Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a global threat to wheat production. Aegilops tauschii, one of the wheat progenitors, carries the YrAS2388 locus for resistance to Pst on chromosome 4DS. We reveal that YrAS2388 encodes a typical nucleotide oligomerization domain-like receptor (NLR). The Pst-resistant allele YrAS2388R has duplicated 3' untranslated regions and is characterized by alternative splicing in the nucleotide-binding domain. Mutation of the YrAS2388R allele disrupts its resistance to Pst in synthetic hexaploid wheat; transgenic plants with YrAS2388R show resistance to eleven Pst races in common wheat and one race of P. striiformis f. sp. hordei in barley. The YrAS2388R allele occurs only in Ae. tauschii and the Ae. tauschii-derived synthetic wheat; it is absent in 100% (n = 461) of common wheat lines tested. The cloning of YrAS2388R will facilitate breeding for stripe rust resistance in wheat and other Triticeae species

Differentially Private Diffusion Auction: The Single-unit Case

Diffusion auction refers to an emerging paradigm of online marketplace where an auctioneer utilises a social network to attract potential buyers. Diffusion auction poses significant privacy risks. From the auction outcome, it is possible to infer hidden, and potentially sensitive, preferences of buyers. To mitigate such risks, we initiate the study of differential privacy (DP) in diffusion auction mechanisms. DP is a well-established notion of privacy that protects a system against inference attacks. Achieving DP in diffusion auctions is non-trivial as the well-designed auction rules are required to incentivise the buyers to truthfully report their neighbourhood. We study the single-unit case and design two differentially private diffusion mechanisms (DPDMs): recursive DPDM and layered DPDM. We prove that these mechanisms guarantee differential privacy, incentive compatibility and individual rationality for both valuations and neighbourhood. We then empirically compare their performance on real and synthetic datasets

How does ego-depletion affect the reemployment intention of county public sector retirees? The mediating effect of psychological ownership and the moderating effect of social trust

In recent years, with the increasingly severe situation of population aging in China, it had become one of the important ways to implement the active aging strategy and promote the reemployment of the retired young healthy elderly people. At present, there were quite a number of young public sector workers in China who were still able to work at the county level and were forced to quit their jobs due to retirement. To promote the reemployment of these precious labor resources was of great significance to alleviate the problem of labor shortage in China. In this paper, ego-depletion was divided into three dimensions: cognitive depletion, emotional depletion and behavioral depletion. The influence of various dimensions of ego-depletion on reemployment intention was systematically analyzed. The effect of psychological ownership and social trust in the relationship between ego-depletion and reemployment intention was analyzed. The results showed that: (1) All dimensions of ego-depletion had a negative effect on the reemployment intention of younger public sector retirees. The effects of each dimension were as follows: cognitive depletion > behavioral depletion > emotional depletion; (2) Cognitive depletion and emotional depletion had significant negative effects on psychological ownership; Behavior depletion had no significant effect on psychological ownership. (3) Psychological ownership played a mediating effect in the inhibition of reemployment intention by cognitive depletion and emotional depletion; There was no effect on the effect of behavioral depletion on reemployment intention; (4) Social trust positively moderated the relationship between ego-depletion and psychological ownership; In addition, social trust positively moderated the effects of cognitive depletion and behavioral depletion on reemployment intention, but had no moderating effect on the relationship between emotional depletion and reemployment intention

Decreased oocyte quality in patients with endometriosis is closely related to abnormal granulosa cells

Infertility and menstrual abnormalities in endometriosis patients are frequently caused by aberrant follicular growth or a reduced ovarian reserve. Endometriosis typically does not directly harm the oocyte, but rather inhibits the function of granulosa cells, resulting in a decrease in oocyte quality. Granulosa cells, as oocyte nanny cells, can regulate meiosis, provide the most basic resources required for oocyte development, and influence ovulation. Endometriosis affects oocyte development and quality by causing granulosa cells apoptosis, inflammation, oxidative stress, steroid synthesis obstacle, and aberrant mitochondrial energy metabolism. These aberrant states frequently interact with one another, however there is currently relatively little research in this field to understand the mechanism of linkage between abnormal states

Applications and Therapeutic Actions of Complementary and Alternative Medicine for Women with Genital Infection

Genital infection is a common worldwide disease among females with clinical features such as bilateral lower abdominal tenderness, abnormal vaginal or cervical discharge, fever, abnormal vaginal bleeding, dyspareunia, vaginal itching, and adnexal tenderness, which can significantly impair women’s health and quality of life. Genital infection is commonly treated with antibiotics, leading to an imbalance in gut flora due to prolonged use of antibiotics. Therefore, it is necessary to discover safe and efficacious alternative treatment strategies for patients with genital infection. Complementary and alternative medicine (CAM) is becoming increasingly prevalent among women with genital infection. CAM has interested the western mainstream medical community because of its less invasive, safe, effective, economical, and convenient therapies. CAM focuses on the prevention and treatment of disease and has become an important force in treating chronic disease. During the last few decades, the popularity of CAM has gradually increased. To further understand the efficacy of CAM in treating genital infection, our paper will review the current progress of treating genital infection including vulvitis, vaginitis, cervicitis, and pelvic inflammatory disease (PID) with CAM therapies. Several CAM strategies including traditional Chinese medicine (TCM), acupuncture, Psychology interference, and physical therapy are introduced in this review.</jats:p