Hydrogen Storage: State-of-the-Art and Future Perspective.

Abstract not availableJRC.F-Institute for Energy (Petten

A new perspective on open innovation: established and new technology firms in UK bio‐pharmaceuticals

The aim of this paper is to explore open innovation (OI) implementation and its impact on firm innovation performance in sectors experiencing technological discontinuities. The paper employs the framework of inbound, outbound and coupled OI to identify processes reflecting sourcing, externalising and exchanging knowledge across organisational boundaries on upstream and downstream innovation activities and explores their impact on the innovation performance of new and established technology firms. The empirical setting is the UK bio‐pharmaceuticals sector during 1991 and 2001, a paradigmatic era of discontinous change and intensified OI implementation. First, our findings show that new technology firms (NTFs) and established technology firms (ETFs) differ in their extent and patterns of inbound, outbound and coupled OI, reflecting that they implement OI to manage their competences in light of technological change. Second, we identify a complex and multifaceted relationship between OI and patenting performance, with NTFs experiencing enhanced performance from some OI processes while ETFs experiencing challenges. The paper suggests that delineating OI into inbound, outbound and coupled, along upstream and downstream activities, offers a deeper understanding of the role of OI in innovation, guiding selective implementation in pursuing enhanced innovation performance during periods of discontinuous technological change

L2 acquisition of interrogative and relative clauses by Greek learners of English: that-trace effects and subject-object extraction

The present paper investigates the L2 acquisition of wh-interrogative and relative clauses in English by L1 Greek speakers. Based on SLA theories that support partial availability of Universal Grammar, such as the No Parameter Resetting Hypothesis (Tsimpli & Roussou 1991) and the Interpretability Hypothesis (Tsimpli & Dimitrakopoulou 2007), the study explores the nature of Interlanguage representation and the role of uninterpretable features with respect to specific grammatical properties: that-trace effects, Agreement, wh-subject and object extractions in interrogative and relative clauses. The acquisitional data were collected by intermediate and advanced Greek learners of English (n=40) and a control group of native speakers (n=10) using a grammaticality judgement task (consisting of 80 sentences). The results of the empirical study show that both intermediate and advanced L2 learners accept the ungrammatical that-trace structures in subj.wh-interrogatives due to the uninterpretability of the feature involved. L2 learners seem to transfer the properties of subj.-verb agreement in Greek to English L2. Moreover, no developmental change is observed with respect to Subj.(+that) interrogatives. This paper attempts to confirm the claim that uninterpretable features are inaccessible to L2 learners and cause learnability problems in L2 acquisition

Tau deletion prevents stress-induced dendritic atrophy in prefrontal cortex: role of synaptic mitochondria

Tau protein in dendrites and synapses has been recently implicated in synaptic degeneration and neuronal malfunction. Chronic stress, awell-known inducer of neuronal/synaptic atrophy, triggers hyperphosphorylation of Tau protein and cognitive deficits. However, the cause–effect relationship between these events remains to be established. To test the involvement of Tau in stress-induced impairments of cognition,we investigated the impact of stress on cognitive behavior, neuronal structure, and the synaptic proteome in the prefrontal cortex (PFC) of Tau knock-out (Tau-KO) and wild-type (WT) mice. Whereas exposure to chronic stress resulted in atrophy of apical dendrites and spine loss in PFC neurons as well as significant impairments in working memory in WT mice, such changes were absent in Tau-KO animals. Quantitative proteomic analysis of PFC synaptosomal fractions, combined with transmission electron microscopy analysis, suggested a prominent role for mitochondria in the regulation of the effects of stress. Specifically, chronically stressed animals exhibit Tau-dependent alterations in the levels of proteins involved in mitochondrial transport and oxidative phosphorylation aswell as in the synaptic localization of mitochondria in PFC. These findings provide evidence for a causal role of Tau in mediating stress-elicited neuronal atrophy and cognitive impairment and indicate that Tau may exert its effects through synaptic mitochondria.This work was funded by the Portuguese Foundation for Science & Technology (FCT, grant number NMC-113934 to I.S.), the EU Consortium Switchbox (grant number Health-FP7-2010-259772 to O.F.X.A. and N.S.), the Deutsche Forschungsgemeinschaft (grant number FI 1895/1-1 to M.D.F.) and the Max Planck Society (M.D.F., G.M., C.W.T., and O.F.X.A.). In addition, this workwas also co-financed by the Portuguese North Regional Operational Program (ON.2 – O Novo Norte) under the National Strategic Reference Framework (QREN), through the European Regional Development Fund (FEDER) (N.S.). S.L. and I.S. are holders of FCT Fellowships. J.V-S. is a recipient of a PhD fellowship (PD/ BD/105938/2014) of the University of Minho MD/PhD Program funded by FCT.info:eu-repo/semantics/publishedVersio

R&D collaborations: Is diversity enhancing innovation performance?

We develop a theoretical framework which builds on the existence of a feedback loop relationship between internal innovation efforts and the diversity of types of R&D collaborations. Such a feedback loop allows for decomposing the total effects of both internal and external knowledge sources on innovation performance in direct and indirect effects. We argue that such feedback loop lies in the heart of the interplay between the benefits and costs associated with generating knowledge internally and accessing knowledge from diverse external knowledge sources. In particular we argue that anticipated benefits from accessing knowledge from diverse external knowledge sources may be outweighed by (i) costs associated with accessing increasingly diverse knowledge through collaboration and (ii) a negative network effect on firms' internal innovation efforts. We employ Structural Equation Modelling on a bespoke dataset of Greek R&D active manufacturing firms; empirical results confirm the existence of an idiosyncratic feedback loop relationship and show that internal innovation efforts positively influence firm innovation performance. On the other hand, diversity in external collaborations has a negative impact on internal innovation efforts, elevating the importance of the optimal balance between internal R&D investments and the diversity of R&D collaborations. The same picture emerges when examining the corresponding direct and indirect effects of internal and external knowledge sources on innovation performance

Ketamine's antidepressant effect is mediated by energy metabolism and antioxidant defense system.

Fewer than 50% of all patients with major depressive disorder (MDD) treated with currently available antidepressants (ADs) show full remission. Moreover, about one third of the patients suffering from MDD does not respond to conventional ADs and develop treatment-resistant depression (TRD). Ketamine, a non-competitive, voltage-dependent N-Methyl-D-aspartate receptor (NMDAR) antagonist, has been shown to have a rapid antidepressant effect, especially in patients suffering from TRD. Hippocampi of ketamine-treated mice were analysed by metabolome and proteome profiling to delineate ketamine treatment-affected molecular pathways and biosignatures. Our data implicate mitochondrial energy metabolism and the antioxidant defense system as downstream effectors of the ketamine response. Specifically, ketamine tended to downregulate the adenosine triphosphate (ATP)/adenosine diphosphate (ADP) metabolite ratio which strongly correlated with forced swim test (FST) floating time. Furthermore, we found increased levels of enzymes that are part of the 'oxidative phosphorylation' (OXPHOS) pathway. Our study also suggests that ketamine causes less protein damage by rapidly decreasing reactive oxygen species (ROS) production and lend further support to the hypothesis that mitochondria have a critical role for mediating antidepressant action including the rapid ketamine response

Welfare and productivity of growing rabbits in collective housing systems

Public opinion asks for adopting welfare-friendly techniques during rearing of farm animals. The housing system in pairs within wire-net cages (2 rabbits/cage) or small groups (4-8 rabbits/cage), which is currently implemented for rabbits should change and alternative pen housing should be adopted to ameliorate animal welfare in respect of growth performance and meat traits. The farming environment should change and be adjusted to the presence of a large group of animals with diversified social relationships that could be influenced by gender composition and slaughter age. The passage from the conventional housing systems to the new alternative ones cannot be achievable without previous research on the effects of this new system on growth performance and welfare of animals. To achieve this general goal, four trials have been conducted under the present thesis and the summaries of the results obtained are reported below: Trial No. 1-Welfare and productivity of growing rabbits housed in bicellular cages and collective pens Trial No. 2 - Effect of floor type, stocking density, slaughter age, and gender on welfare and productivity of growing rabbits housed in collective pens Trial No. 3–Effect of environmental enrichment and slaughter age on welfare and productivity of growing rabbits housed in collective pens Trial No.4–Effect of different gender composition and floor combinations on welfare and productivity of growing rabbits housed in collective pens On the base of the results of the present Thesis, pen housing may be a succesfull way for growing rabbits giving productive results comparable with those obtained in the current cage systems, but only if housing conditions are suitable for the animal

Traitement des mélanges par le système trigéminal

Le système trigéminal –tout comme l’olfaction et la gustation– est un sens chimique qui permet la perception des informations chimiosensorielles de notre environnement. Contrairement à l’olfaction et à la gustation, notre connaissance du traitement des mélanges par le système trigéminal est limitée. Nous avons donc utilisé des mélanges de trois agonistes relativement spécifiques à des récepteurs (eucalyptol, agoniste TRPM8; aldéhyde cinnamique, agoniste TRPA1 ; camphre, agoniste TRPV1) et d’une odeur pure (alcool phényléthylique) dans différentes proportions afin de déterminer les dimensions de base de la perception trigéminale. Quatre dimensions principales se sont avérées pertinentes: l’intensité, la sensation de chaleur, la sensation de froid et la douleur. Nous avons utilisé ces dimensions pour étudier la perception de mélanges et de combinaisons dans différentes proportions d’un stimulus qui procure une sensation de froid (eucalyptol) et d’un stimulus qui procure une sensation de chaleur (aldéhyde cinnamique). Les résultats indiquent que les mélanges obtiennent généralement des scores plus élevés que les combinaisons sur les dimensions « intensité », « sensation de chaleur » et « douleur » alors que les combinaisons obtiennent des scores plus élevés sur la dimension « sensation de froid ». Ces résultats suggèrent des interactions spécifiques pour les différentes dimensions de la perception trigéminale. Nous en venons à la conclusion d’un effet d’additivité pour les mélanges sur les dimensions « intensité », « sensation de chaleur » et « douleur » alors que nous observons plutôt un effet de suppression de la perception de froid pour les deux stimuli dans les mélanges, ce qui semble indiquer des interactions particulières pouvant prendre place aux niveaux périphérique ou central.The trigeminal system – along with smell and taste – is a chemical sense that allows the perception of chemosensory information in our environment. However, contrary to smell and taste, we lack knowledge of the trigeminal processing of mixtures. We therefore investigated trigeminal perception using mixtures of three relatively receptor specific agonists (eucalyptol, TRPM8 agonist; cinnamaldehyde, TRPA1 agonist and camphor, TRPV1 agonist) together with one control odor (phenyl ethyl alcohol or PEA) in different proportions to determine basic dimensions of trigeminal perception. Four main dimensions were proven relevant to trigeminal perception: intensity, warmth sensation, cold sensation and pain sensation. Subsequently, we used these dimensions to investigate perception of mixtures and combinations of trigeminal stimuli using different concentrations of a cooling stimulus (eucalyptol) with a stimulus which evokes warmth perception (cinnamaldehyde). Results showed mixtures generally yielded higher ratings than combinations on the trigeminal dimensions “intensity”, “warm” and “painful” whereas combinations yielded higher ratings than mixtures on the trigeminal dimension “cold”. These results suggest dimension specific interactions in the perception of trigeminal mixtures. For the mixtures we used, we reach the conclusion of additivity for the dimensions “intensity”, “warm” and “painful”; we observed suppression of the cold perception of both stimuli in mixtures suggesting particular interactions which may take place on peripheral or central levels