Respiratory Syncytial Virus Binds and Undergoes Transcription in Neutrophils From the Blood and Airways of Infants With Severe Bronchiolitis

Background. Neutrophils are the predominant cell in the lung inflammatory infiltrate of infants with respiratory syncytial virus (RSV) bronchiolitis. Although it has previously been shown that neutrophils from both blood and bronchoalveolar lavage (BAL) are activated, little is understood about their role in response to RSV infection. This study investigated whether RSV proteins and mRNA are present in neutrophils from blood and BAL of infected infants

Rapid subtyping of influenza A virus isolates by membrane fluorescence

During the winter of 1977-1978 three influenza A virus serotypes (A/Vic/3/75, A/Texas/1/77 [both H3N2], and A/USSR/90/77 [H1N1]) circulated in Denver, offering us the opportunity to apply fluorescent antibody techniques to the specific identification of these viruses. Surface antigens of infected, unfixed primary monkey kidney cells were stained in suspension by an indirect immunofluorescence technique with anti-H3N2 and anti-H1N1 antisera. In tests of cells infected with known viruses, the members of the H3N2 family could not be distinguished from one another, but were easily distinguished from H1N1 strains. A total of 101 hemadsorption-positive clinical specimens were evaluated over a 6-month period. Forty-five of 48 influenza A H3N2 and 24 of 29 H1N1 specimens confirmed by hemagglutination inhibition were correctly identified by membrane fluorescence of cultured cells, with no misidentifications among influenza strains and with 1 false positive among 24 non-influenza isolates. The average time to identification by this technique was 4 days compared to 7 days by hemagglutination inhibition. Live cell membrane fluorescence is a simple, rapid, and accurate method for identifying and grouping influenza A viruses.</jats:p

Cryopreservation of virus-infected cells for use in the fluorescent antibody to membrane antigen test

Tissue culture cells infected with varicella-zoster, respiratory syncytial, para-influenza types 1, 2, and 3, and influenza A/Texas/1/77 and A/USSR/90/77 viruses were exposed to ultraviolet light and frozen in the presence of a final concentration of 20% glycerol. These cells were quick thawed at 37 degrees C and compared with freshly prepared, living infected tissue culture cells in assays of fluorescence antibodies to membrane antigens of the infecting agents in serum, nasal wash secretions, colostrum, and breast milk. Frozen cells performed as efficiently as fresh cells as targets and retained their activity for long periods of time. Cryopreservation combined with photoinactivation of infected target cells allows this useful antibody test to be performed in routine serology laboratories.</jats:p