Cytochrome cM decreases photosynthesis under photomixotrophy in Synechocystis sp. PCC 6803

Photomixotrophy is a metabolic state that enables photosynthetic microorganisms to simultaneously perform photosynthesis and metabolism of imported organic carbon substrates. This process is complicated in cyanobacteria, since many, including Synechocystis sp. PCC 6803, conduct photosynthesis and respiration in an interlinked thylakoid membrane electron transport chain. Under photomixotrophy, the cell must therefore tightly regulate electron fluxes from photosynthetic and respiratory complexes. In this study, we demonstrate, via characterization of photosynthetic apparatus and the proteome, that photomixotrophic growth results in a gradual inhibition of QA- reoxidation in wild-type Synechocystis, which largely decreases photosynthesis over 3 d of growth. This process is circumvented by deleting the gene encoding cytochrome cM (CytM), a cryptic c-type heme protein widespread in cyanobacteria. The ΔCytM strain maintained active photosynthesis over the 3-d period, demonstrated by high photosynthetic O2 and CO2 fluxes and effective yields of PSI and PSII. Overall, this resulted in a higher growth rate compared to that of the wild type, which was maintained by accumulation of proteins involved in phosphate and metal uptake, and cofactor biosynthetic enzymes. While the exact role of CytM has not been determined, a mutant deficient in the thylakoid-localized respiratory terminal oxidases and CytM (ΔCox/Cyd/CytM) displayed a phenotype similar to that of ΔCytM under photomixotrophy. This, in combination with other physiological data, and in contrast to a previous hypothesis, suggests that CytM does not transfer electrons to these complexes. In summary, our data suggest that CytM may have a regulatory role in photomixotrophy by modulating the photosynthetic capacity of cells

New optical filamentary structures in Pegasus

Deep Halpha+[N II] CCD images have been obtained in the area of the Pegasus Constellation. The resulting mosaic covers an extent of ~7.5 degr x ~8.5 degr and filamentary and diffuse emission is discovered. Several long filaments (up to ~1 degr) are found within the field, while diffuse emission is present mainly in the central and northern areas. The filaments show variations in the intensity along their extent suggesting inhomogeneous interstellar clouds. Faint soft X-ray emission is also detected in the ROSAT All-Sky Survey. It is mainly concentrated in the central areas of our field and overlaps the optical emission. The low ionization images of [S II] of selected areas mainly show faint diffuse emission, while in the medium ionization images of [O III] diffuse and faint filamentary structures are present. Spectrophotometric observations have been performed on the brightest filaments and indicate emission from photoionized or shock-heated gas. The sulfur line ratios indicate electron densities below ~600 cm^{-3}, while the absolute Halpha emission lies in the range of 1.1 - 8.8 x 10^{-17} erg s^{-1} cm^{-2} arcsec^{-2}. The detected optical line emission could be part of a single or multiple supernova explosions.Comment: 13 pages, 7 figures, accepted for publication in A&

Improving self-referral for diabetes care following hypoglycaemic emergencies: a feasibility study with linked patient data analysis

Background  Hypoglycaemia is a common and potentially life threatening consequence of insulin and sulphonylurea treated Diabetes. Some severe hypoglycaemic events result in emergency ambulance attendance. Many of these patients are treated at home and do not require immediate transportation to an Emergency Department. However only 27-37% of patients then follow up their care with a diabetes specialist. Consequently repeat severe hypoglycaemic events occur.  Methods  The intervention was implemented for 8months, using a prospective cohort design with a historic control, in one Scottish Health Board in 2012. Data was collected using postal survey questionnaires to patients and ambulance clinicians, telephone survey follow-up questions to patients. Scottish Ambulance Service electronic records were linked with the SCI-Diabetes database of patient records to enable objective measurement of follow-up behaviour.  Results  Ambulance clinicians’ (n = 92) awareness of the intervention was high and both the prompt card and telephone call components of the intervention were delivered to most eligible patients. The intervention was perceived as highly acceptable to patients (n = 37), and very useful by both patients and ambulance clinicians. However, comparison of patient follow-up behaviours using linked-data (n = 205), suggest that the intervention was unsuccessful in improving rates of patients’ following up their care.  Conclusions  This study shows that the intervention is implementable, highly acceptable to patients, and considered very useful by both patients and ambulance clinicians. However, preliminary evidence of effectiveness is not encouraging. The study’s novel use of linking existing clinical data for outcome measurement exposed challenges in the feasibility of using this data for intervention development and evaluation. Future research should examine challenges to the successful testing and effectiveness of the intervention. Revisions are likely to be required, both to study design and the optimisation of the intervention’s content and components

Improving post-hypoglycaemic patient safety in the prehospital environment: a systematic review

To determine the extent to which post-hypoglycaemic patients with diabetes, who are prescribed oral hypoglycaemic agents (OHA) are at risk of repeat hypoglycaemic events (RHE) after being treated in the prehospital environment and whether they should be transported to hospital regardless of their post-treatment response, a systematic literature review was carried out using an overlapping retrieval strategy that included both published and unpublished literature. Retrieved papers were reviewed by each author for inclusion. Disagreements regarding inclusion were resolved through discussion. Ninety-eight papers and other relevant material were retrieved using the developed search strategy. Twenty-three papers and other relevant material were included in the final review. A narrative synthesis of the findings is presented. Although several case reports demonstrate the risks associated with repeat or prolonged hypoglycaemia, the review was unable to locate any specific high quality research in this area. Consequently, caution is required in interpreting the findings of the studies. Post-hypoglycaemic patients treated in the prehospital environment have a 2–7% risk of experiencing a RHE within 48 h. The literature retrieved in this study recognises the potential for OHA to cause RHE. However, the extent to which this occurs in practice remains unknown. This lack of evidence has led to the recommendation that conservative management, through admission to hospital, is appropriate. The review concludes with recommendations for both practice and research

Stellar Nucleosynthesis in the Hyades Open Cluster

We report a comprehensive light element (Li, C, N, O, Na, Mg, and Al) abundance analysis of three solar-type main sequence (MS) dwarfs and three red giant branch (RGB) clump stars in the Hyades open cluster using high-resolution and high signal-to-noise spectroscopy. For each group (MS or RGB), the CNO abundances are found to be in excellent star-to-star agreement. Our results confirm that the giants have undergone the first dredge-up and that material processed by the CN cycle has been mixed to the surface layers. The observed abundances are compared to predictions of a standard stellar model based on the Clemson-American University of Beirut (CAUB) stellar evolution code. The model reproduces the observed evolution of the N and O abundances, as well as the previously derived 12C/13C ratio, but it fails to predict by a factor of 1.5 the observed level of 12C depletion. Li abundances are derived to determine if non-canonical extra mixing has occurred in the Hyades giants. The Li abundance of the giant gamma Tau is in good accord with the predicted level of surface Li dilution, but a ~0.35 dex spread in the giant Li abundances is found and cannot be explained by the stellar model. Possible sources of the spread are discussed; however, it is apparent that the differential mechanism responsible for the Li dispersion must be unrelated to the uniformly low 12C abundances of the giants. Na, Mg, and Al abundances are derived as an additional test of our stellar model. All three elements are found to be overabundant by 0.2-0.5 dex in the giants relative to the dwarfs. Such large enhancements of these elements are not predicted by the stellar model, and non-LTE effects significantly larger (and, in some cases, of opposite sign) than those implied by extant literature calculations are the most likely cause.Comment: 40 pages, 6 figures, 6 tables; accepted by Ap

The 0.4-Mo Eclipsing Binary CU Cancri: Absolute Dimensions, Comparison with Evolutionary Models and Possible Evidence for a Circumstellar Dust Disk

Photometric observations in the R and I bands of the detached M-type double-lined eclipsing binary CU Cnc have been acquired and analysed. The photometric elements obtained from the analysis of the light curves have been combined with an existing spectroscopic solution to yield high-precision (errors<2%) absolute dimensions: M_A=0.4333+/-0.0017 Mo, M_B=0.3980+/-0.0014 Mo, R_A=0.4317+/-0.0052 Ro, and R_B=0.3908+/-0.0094 Ro. The mean effective temperature of the system has been estimated to be Teff=3140+/-150 K by comparing multi-band photometry with synthetic colors computed from model atmospheres. Additionally, we have been able to obtain an estimate for the age (~320 Myr) and chemical composition ([Fe/H]~0.0) of the binary system through its membership of the Castor moving group. With all these observational constraints, we have carried out a critical test of recent stellar models for low-mass stars. The comparison reveals that most evolutionary models underestimate the radius of the stars by as much as 10%, thus confirming the trend observed by Torres & Ribas (2002) for YY Gem and V818 Tau. In the mass-absolute magnitude diagram, CU Cnc is observed to be dimmer than other stars of the same mass. After ruling out a number of different scenarios, the apparent faintness of CU Cnc can be explained if its components are some 10% cooler than similar-mass stars or if there is some source of circumstellar dust absorption. The latter could be a tantalizing indirect evidence for a coplanar (Vega-like) dusty disk around this relatively young M-type binary.Comment: 14 pages, 5 figures, 6 tables. Accepted for publication in A&A. Tables 1 and 2 available in electronic form at the CDS after publicatio

Implementation of routine outcome measurement in child and adolescent mental health services in the United Kingdom: a critical perspective

The aim of this commentary is to provide an overview of clinical outcome measures that are currently recommended for use in UK Child and Adolescent Mental Health Services (CAMHS), focusing on measures that are applicable across a wide range of conditions with established validity and reliability, or innovative in their design. We also provide an overview of the barriers and drivers to the use of Routine Outcome Measurement (ROM) in clinical practice

Early X-ray and optical observations of the soft gamma-ray repeater SGR 0418+5729

Emission of two short hard X-ray bursts on 2009 June 5 disclosed the existence of a new soft gamma-ray repeater, now catalogued as SGR 0418+5729. After a few days, X-ray pulsations at a period of 9.1 s were discovered in its persistent emission. SGR 0418+5729 was monitored almost since its discovery with the Rossi X-ray Timing Explorer (2-10 keV energy range) and observed many times with Swift (0.2-10 keV). The source persistent X-ray emission faded by a factor 10 in about 160 days, with a steepening in the decay about 19 days after the activation. The X-ray spectrum is well described by a simple absorbed blackbody, with a temperature decreasing in time. A phase-coherent timing solution over the 160 day time span yielded no evidence for any significant evolution of the spin period, implying a 3-sigma upper limit of 1.1E-13 s/s on the period derivative and of 3E+13 G on the surface dipole magnetic field. Phase-resolved spectroscopy provided evidence for a significant variation of the spectrum as a function of the stellar rotation, pointing to the presence of two emitting caps, one of which became hotter during the outburst. Finally, a deep observation of the field of SGR 0418+5729 with the new Gran Telescopio Canarias 10.4-m telescope allowed us to set an upper limit on the source optical flux of i'>25.1 mag, corresponding to an X-ray-to-optical flux ratio exceeding 10000, consistent with the characteristics of other magnetars.Comment: The paper (10 pages) contains 6 colour figures and 2 tables; accepted for publication in MNRA

On the influence of the companion star in Eta Carinae: 2D radiative transfer modeling of the ultraviolet and optical spectra

We present 2D radiative transfer modeling of the Eta Carinae binary system accounting for the presence of a wind-wind collision (WWC) cavity carved in the optically-thick wind of the primary star. By comparing synthetic line profiles with HST/STIS spectra obtained near apastron, we show that the WWC cavity has a strong influence on multi-wavelength diagnostics. This influence is regulated by the modification of the optical depth in the continuum and spectral lines. We find that H-alpha, H-beta, and Fe II lines are the most affected by the WWC cavity, since they form over a large volume of the primary wind. These spectral lines depend on latitude and azimuth since, according to the orientation of the cavity, different velocity regions of a spectral line are affected. For 2D models with orientation corresponding to orbital inclination angle 110deg < i < 140deg and longitude of periastron 210deg < omega < 330deg, the blueshifted and zero-velocity regions of the line profiles are the most affected. These orbital orientations are required to simultaneously fit the UV and optical spectrum of Eta Car, for a half-opening angle of the cavity in the range 50-70deg. We find that the excess P-Cygni absorption seen in H-alpha, H-beta and optical Fe II lines in spherical models becomes much weaker or absent in the 2D models, in agreement with the observations. The observed UV spectrum of Eta Car, dominated by Fe II absorption lines, is superbly reproduced by our 2D cavity models. Small discrepancies still remain, as H-gamma and H-delta absorptions are overestimated by our models. We suggest that photoionization of the wind of the primary by the hot companion star is responsible for the weak absorption seen in these lines. Our CMFGEN models indicate that the primary star has a mass-loss rate of 8.5x10e-4 Msun/yr and wind terminal velocity of 420 km/s around the 2000 apastron.Comment: 20 pages, 14 figures, accepted for publication in MNRA

Minding the gaps : using narrative account to explore people’s experiences of using a voluntary sector mental health counselling service

Introduction: In the UK almost 50% of illness diagnosed among working age adults is mental distress, with depression and chronic anxiety cited as the two most prevalent psychological illnesses. However, only 24% of those who experience anxiety and depression, consistent with diagnoses, receive National Health Service (NHS) interventions. Effective mental health care is predicated on understanding the lived experiences of those using services in order to provide sensitively-attuned therapy. An understanding of the process of counselling and what makes it effective will only be achieved through hearing the voices of service users. However, with regard to counselling, the literature foregrounding the perspectives of those using services remains sparse. Method: This paper reports on a qualitative research project, adopting a case study approach, the aim of which was to explore detailed narratives of the experiences of people who had used a voluntary sector mental health counselling service in order to elicit the strengths and/or opportunities for improvement of the service. Twelve participants, five males and seven females, were interviewed on a one to one basis and six themes were identified; Mindful of the Gap; Easing like Sunday Morning; Magic Moments; Love is in the Air; Lighting up a Future and Following up the Changes: Spreading the Word. Results: Findings suggest the service is beneficial, with therapeutic interventions being tailor-made to meet the person’s needs and one which ensures a safe environment and compassionate care for those in distress