Restoration of biogeomorphic landscapes by creating ‘windows of opportunity’

Coastal and arid ‘biogeomorphic’ ecosystems are harsh environments where physical disturbances constantly destroy colonizing vegetation by remobilizing and overturning the sediment. These forces can be impeded by dense stands of vegetation, whose canopy slows the flow of wind and water, and whose roots constrict and immobilize the sediment. But in order for vegetation to take control of these processes, young pioneer plants must first somehow survive the initially extreme environment. How is that possible?The ‘window of opportunity’ concept suggests an answer to this question: the occasionally successful establishment of pioneer vegetation in hostile environments occurs because the state of the environment is non-constant. In periods of unusually calm conditions, establishing organisms can develop the tolerances required to survive once disturbances reappear. This conceptual framework identifies three key parameters that determine how likely an environmental is to experience a ‘window’ between disturbances that will be sufficient to facilitate pioneer establishment. These are: (1) the intensity of disturbances, (2) the interval of calm conditions between disturbances, (3) and the rate at which an organism develops tolerance to disturbances. Based on this premise, the brief manipulation of any of these three parameters should create opportunities for pioneer establishment. In this thesis, we first study how observed natural establishment events fit within the ‘window-of-opportunity’ framework. Then, using this knowledge, we develop restoration tools that recreate the conditions observed in natural establishment events to artificially provoke pioneer recruitment

Identifying Individual T Cell Receptors of Optimal Avidity for Tumor Antigens.

Cytotoxic T cells recognize, via their T cell receptors (TCRs), small antigenic peptides presented by the major histocompatibility complex (pMHC) on the surface of professional antigen-presenting cells and infected or malignant cells. The efficiency of T cell triggering critically depends on TCR binding to cognate pMHC, i.e., the TCR-pMHC structural avidity. The binding and kinetic attributes of this interaction are key parameters for protective T cell-mediated immunity, with stronger TCR-pMHC interactions conferring superior T cell activation and responsiveness than weaker ones. However, high-avidity TCRs are not always available, particularly among self/tumor antigen-specific T cells, most of which are eliminated by central and peripheral deletion mechanisms. Consequently, systematic assessment of T cell avidity can greatly help distinguishing protective from non-protective T cells. Here, we review novel strategies to assess TCR-pMHC interaction kinetics, enabling the identification of the functionally most-relevant T cells. We also discuss the significance of these technologies in determining which cells within a naturally occurring polyclonal tumor-specific T cell response would offer the best clinical benefit for use in adoptive therapies, with or without T cell engineering

Salt marsh fragmentation in a mesotidal estuary:Implications for medium to long-term management

During the last decades many salt marshes worldwide have suffered important losses in their extent and associated ecosystem services. The salt marshes of San Vicente de la Barquera estuary (N Spain) are a clear example of this, with a drastic reduction in vegetation surface over the last 60 years. This paper provides insights into the main factors controlling salt marsh functioning in sheltered estuarine areas. Regional and local factors have been disaggregated to identify the main drivers controlling the functioning of the salt marsh to develop appropriate management measures according to the evolution of the system. These factors have been studied in their spatial context through detailed maps of change in vegetation cover combined with topographic data obtained from UAV and RTK-DGPS surveys. The results demonstrate that in this estuary the salt marsh area is declining following a fragmentation process. No clear pattern of vegetation loss/gain with elevation has been identified. However, the results point to increased hydrodynamic stress in the area, with stronger currents inside the estuary. This is probably the major factor responsible for the decline of the salt marshes in the San Vicente de la Barquera estuary. Furthermore, several human interventions during the 20th century (local drivers) have also probably contributed to a lower resilience against SLR (regional driver). This work demonstrates that both natural and human drivers of change need to be considered when characterizing the evolution of salt marshes, wherever efficient management strategies need to be designed

Elevated micro-topography boosts growth rates in <i>Salicornia procumbens</i> by amplifying a tidally driven oxygen pump:Implications for natural recruitment and restoration

• Background and Aims: The growth rate of pioneer species is known to be a critical component determining recruitment success of marsh seedlings on tidal flats. By accelerating growth, recruits can reach a larger size at an earlier date, which reduces the length of the disturbance-free window required for successful establishment. Therefore, the pursuit of natural mechanisms that accelerate growth rates at a local scale may lead to a better understanding of the circumstances under which new establishment occurs, and may suggest new insights with which to perform restoration. This study explores how and why changes in local sediment elevation modify the growth rate of recruiting salt marsh pioneers. • Methods: A mesocosm experiment was designed in which the annual salt marsh pioneer Salicornia procumbens was grown over a series of raised, flat and lowered sediment surfaces, under a variety of tidal inundation regimes and in vertically draining or un-draining sediment. Additional physical tests quantified the effects of these treatments on sediment water-logging and oxygen dynamics, including the use of a planar optode experiment. • Key Results: In this study, the elevation of sediment micro-topography by 2 cm was the overwhelming driver of plant growth rates. Seedlings grew on average 25 % faster on raised surfaces, which represented a significant increase when compared to other groups. Changes in growth aligned well with the amplifying effect of raised sediment beds on a tidally episodic oxygenation process wherein sediment pore spaces were refreshed by oxygen-rich water at the onset of high tide. • Conclusions: Overall, the present study suggests this tidally driven oxygen pump as an explanation for commonly observed natural patterns in salt marsh recruitment near drainage channels and atop raised sediment mounds and reveals a promising way forward to promote the establishment of pioneers in the field

Salt marshes for nature-based flood defense:Sediment type, drainage, and vegetation drive the development of strong sediment beds

In face of sea-level rise and increasing risks for storm impacts on shorelines, there is a growing demand for developing nature-based flood defenses, for example by restoring or creating salt marshes in front of engineered structures such as dikes. However, salt marshes can only optimally provide flood defense if their sediment beds are erosion resistant, even under very high flow velocities. It remains unknown how fast sediment strength develops in marshes restored or created for nature-based flood defense. Therefore, this study investigated how 1) sediment type, 2) tidal drainage depth and duration, and 3) pioneer vegetation species drive the development rate of sediment strength. A controlled experiment was set up with pots filled with two sediment types, which were either left bare or planted with Spartina anglica or Scirpus maritimus, two dominant salt marsh pioneers in NW Europe. All treatments were subjected to four different tidal regimes with different tidal drainage depth and duration. The results showed that sandy mud (with a 37% silt and clay fraction) led to much stronger sediments than fine mud (with a 77% silt and clay fraction). Sediment strength was higher in the treatments with deeper tidal drainage depth and longer drainage duration. The presence of vegetation increased sediment strength and this effect was stronger with Scirpus maritimus than with Spartina anglica. Plant roots increased sediment strength directly, and the presence of vegetation also seemed to increase sediment strength through enhanced evaporation and transpiration. From these results it can be concluded that to restore or create erosion resistant salt marshes for flood defense, it is essential to ensure that marshes can form at relatively high elevations from well-draining sand-mud mixtures, thereby also ensuring vegetation growth.</p

Emerging trade-offs in saltmarsh ecosystem services under sea-level rise

Coastal defence and carbon storage by coastal wetlands provide an increasingly recognised means of adaptation and mitigation in the face of climate change, yet these same services are threatened by the impacts against which they protect. Our understanding to date tends to consider impacts of rising sea levels on individual marsh services but fails to consider trade-offs and potential interactions between them. For instance, sea-level rise has the potential to enhance carbon storage but may decrease soil stability, erosion resistance and wave-attenuation capacity by creating sub-optimal growth conditions, causing plants to be weaker and smaller. As such, we aim to answer the question: will rising sea levels drive unidirectional impacts, or contrasting ‘trade-offs’, between saltmarsh carbon storage and coastal defence? We conducted a field experiment across increasing inundation durations beyond the usual seaward limit of Spartina anglica growth, mimicking SLR at established vegetation patches, and measured (1) carbon decomposition rates using the teabag index as proxy, (2) sediment stability, and (3) biomechanical traits of S.anglica as proxy for wave-attenuation capacity. With increasing inundation duration, rates of carbon decomposition slowed, supporting carbon storage, and therefore positively impacted climate change mitigation. In contrast, both sediment stability and vegetation stiffness decreased and hence negatively impacted climate change adaptation. Our findings point to a short-term trade-off in climate ecosystem services with SLR, where positive impacts on carbon storage may offset negative impacts on coastal defence services. However, since lower sediment stability increases the chance of marsh erosion under extreme events, this will increase over time the likelihood of sediment resuspension and the ultimate rerelease of previously stored carbon into the marine carbon cycle. Thus, the interaction between ecosystem services may lead to a net negative impact of SLR on climate mitigation services. Overall, we highlight the necessity of taking a multi-service approach to considering the impact of global change effects on ecosystem services.</p

Thermal stress affects bioturbators' burrowing behavior:A mesocosm experiment on common cockles (<i>Cerastoderma edule</i>)

The intensity of marine heatwaves is increasing due to climate change. Heatwaves may affect macroinvertebrates' bioturbating behavior in intertidal areas, thereby altering the deposition-erosion balance at tidal flats. Moreover, small-scale topographic features on tidal flats can create tidal pools during the low tide, thus changing the heat capacity of tidal flats. These pools could then potentially operate as refuge environments during marine heatwaves. We studied behavior responses to heat waves using the well-known bioturbating cockle Cerastoderma edule as a model species. Different temperature regimes (i.e., fluctuating between 20 and 40 °C) and micro-topographies (i.e., presence vs. absence of tidal water pools) were mimicked in a mesocosm experiment with regular tidal regimes. Our results demonstrate that behavioral responses to heat stress strongly depend on the site-specific morphological features. Cockles covered by shallow water pools moved up when exposed to thermal stress, while burrowing deeper into the sediment in the absence of water pools. But in both cases, their migratory behavior increased under heat stress compared to regular ambient treatments. Moreover, long-term cumulative heat stress increased cockles' respiration rates and decreased their health conditions, causing mass mortality after four weeks of gradually increasing heat exposure. Overall, the present findings provide the first insights into how bioturbating behavior on tidal flats may change in response to global warming

The role of seasonality in reproduction of multiannual delayed gametophytes of <i>Saccharina latissima</i>

Delayed gametophytes are able to grow vegetatively for prolonged periods of time. As such, they are potentially very valuable for kelp aquaculture given their great promise in opening up novel opportunities for kelp breeding and farming. However, large-scale application would require more in-depth understanding of how to control reproduction in delayed gametophytes. For newly formed gametophytes, many environmental factors for reproduction have been identified, with key drivers being light intensity, temperature, and the initial gametophyte density. However, the question of whether delayed gametophytes react similarly to these life cycle controls remains open for exploration. In this study, we performed a full factorial experiment on the influences of light intensity, temperature, and density on the reproduction of multiannual delayed gametophytes of Saccharina latissima, during which the number of sporophytes formed was counted. We demonstrate that delayed gametophytes of S. latissima can reliably reproduce sexually after more than a year of vegetative growth, depending on the effects between light intensity and temperature. Under higher light intensities (≥29 µmol photons · m-2 · s-1 ), optimal reproduction was observed at lower temperatures (10.2°C), while at lower light intensities (≤15 µmol photons · m-2 · s-1 ), optimal reproduction was observed at higher temperatures (≥12.6°C). Given the seasonal lag between solar radiation and sea surface temperature in natural systems, these conditions resemble those found during spring (i.e., increasing light intensity with low temperatures) and autumn (i.e., decreasing light intensity with higher temperatures). Seasonality can be used as an aquaculture tool to better control the reproduction of delayed gametophytes