Athletes' perceptions of role ambiguity and coaching competency in sport teams: A multilevel analysis

20 pagesInternational audienceThe purpose of this study was to examine the relationship between athletes' perceptions of role ambiguity and two theoretically derived dimensions of coaching competency (i.e., game strategy and technique competencies). A total of 243 players from 26 teams representing various interdependent sports completed French versions of the Role Ambiguity Scale and the Coaching Competency Scale. Multilevel analyses supported the existence of relationships between the four dimensions of role ambiguity and the two dimensions of coaching competency at both individual and team levels. When the levels were considered jointly, athletes perceiving greater ambiguity in their role in both offensive and defensive contexts were more critical of their coach's capacities to lead their team during competitions and to diagnose or formulate instructions during training sessions. The results also indicated that the dimension of scope of responsibilities was the main contributor to the relationship with coaching competency at an individual level, whereas role evaluation was the main contributor to this relationship at a group level. Findings are discussed in relation to the role episode model, the role ambiguity dimensions involved in the relationships according to the level of analysis considered, and the salience of ambiguity perceptions in the offensive context

Understanding female sport attrition in a stereotypical male sport within the framework of Eccles' expectancy-value model.

International audienceAn empirical research study based upon the expectancy-value model of Eccles and colleagues (1983) investigated the effect of gender role orientations on psychological dimensions of female athletes' sport participation and the likelihood of their continued participation in a stereotypical masculine activity. The model (Eccles et al., 1983) posits that gender role orientation is linked to the intention to persist or discontinue sport participation which is later acted upon indirectly, as mediated by two motivational variables: an individual's perceived competence and the perceived value of the activity. Three models were compared to test this mediation hypothesis with 333 female adolescent handball players in a prospective study. Results from structural equation modeling showed that a fully mediated model better fit the data and that: (1) the masculinity orientation positively predicted value for and perceived competence in handball, whereas the femininity orientation negatively predicted perceived competence; (2) the two motivational variables negatively predicted intention to dropout. And finally such intentions are constituted the more proximal predictors of actual dropout

Athletes' perceptions of role ambiguity and coaching competency in sport teams: A multilevel analysis

20 pagesInternational audienceThe purpose of this study was to examine the relationship between athletes' perceptions of role ambiguity and two theoretically derived dimensions of coaching competency (i.e., game strategy and technique competencies). A total of 243 players from 26 teams representing various interdependent sports completed French versions of the Role Ambiguity Scale and the Coaching Competency Scale. Multilevel analyses supported the existence of relationships between the four dimensions of role ambiguity and the two dimensions of coaching competency at both individual and team levels. When the levels were considered jointly, athletes perceiving greater ambiguity in their role in both offensive and defensive contexts were more critical of their coach's capacities to lead their team during competitions and to diagnose or formulate instructions during training sessions. The results also indicated that the dimension of scope of responsibilities was the main contributor to the relationship with coaching competency at an individual level, whereas role evaluation was the main contributor to this relationship at a group level. Findings are discussed in relation to the role episode model, the role ambiguity dimensions involved in the relationships according to the level of analysis considered, and the salience of ambiguity perceptions in the offensive context

The European AntibotABE Framework Program and Its Update: Development of Innovative Botulinum Antibodies

The goal of the AntiBotABE Program was the development of recombinant antibodies that neutralize botulinum neurotoxins (BoNT) A, B and E. These serotypes are lethal and responsible for most human botulinum cases. To improve therapeutic efficacy, the heavy and light chains (HC and LC) of the three BoNT serotypes were targeted to achieve a synergistic effect (oligoclonal antibodies). For antibody isolation, macaques were immunized with the recombinant and non-toxic BoNT/A, B or E, HC or LC, followed by the generation of immune phage-display libraries. Antibodies were selected from these libraries against the holotoxin and further analyzed in in vitro and ex vivo assays. For each library, the best ex vivo neutralizing antibody fragments were germline-humanized and expressed as immunoglobulin G (IgGs). The IgGs were tested in vivo, in a standardized model of protection, and challenged with toxins obtained from collections of Clostridium strains. Protective antibody combinations against BoNT/A and BoNT/B were evidenced and for BoNT/E, the anti-LC antibody alone was found highly protective. The combination of these five antibodies as an oligoclonal antibody cocktail can be clinically and regulatorily developed while their high “humanness” predicts a high tolerance in humans.Peer reviewe

Neutralization of Botulinum Neurotoxin Type E by a Humanized Antibody

Botulinum neurotoxins (BoNTs) cause botulism and are the deadliest naturally-occurring substances known to humans. BoNTs have been classified as one of the category A agents by the Centers for Disease Control and Prevention, indicating their potential use as bioweapons. To counter bio-threat and naturally-occurring botulism cases, well-tolerated antibodies by humans that neutralize BoNTs are relevant. In our previous work, we showed the neutralizing potential of macaque (Macaca fascicularis)-derived scFv-Fc (scFv-Fc ELC18) by in vitro endopeptidase immunoassay and ex vivo mouse phrenic nerve-hemidiaphragm assay by targeting the light chain of the botulinum neurotoxin type E (BoNT/E). In the present study, we germline-humanized scFv-Fc ELC18 into a full IgG hu8ELC18 to increase its immunotolerance by humans. We demonstrated the protection and prophylaxis capacity of hu8ELC18 against BoNT/E in a mouse model. A concentration of 2.5 ng/mouse of hu8ELC18 protected against 5 mouse lethal dose (MLD) in a mouse protection assay and complete neutralization of 1 LD50 of pure BoNT/E toxin was achieved with 8 ng of hu8ELC18 in mouse paralysis assay. Furthermore, hu8ELC18 protected mice from 5 MLD if injected up to 14 days prior to intraperitoneal BoNT/E administration. This newly-developed humanized IgG is expected to have high tolerance in humans.Peer reviewe

Literature-based discovery of diabetes- and ROS-related targets

Abstract Background Reactive oxygen species (ROS) are known mediators of cellular damage in multiple diseases including diabetic complications. Despite its importance, no comprehensive database is currently available for the genes associated with ROS. Methods We present ROS- and diabetes-related targets (genes/proteins) collected from the biomedical literature through a text mining technology. A web-based literature mining tool, SciMiner, was applied to 1,154 biomedical papers indexed with diabetes and ROS by PubMed to identify relevant targets. Over-represented targets in the ROS-diabetes literature were obtained through comparisons against randomly selected literature. The expression levels of nine genes, selected from the top ranked ROS-diabetes set, were measured in the dorsal root ganglia (DRG) of diabetic and non-diabetic DBA/2J mice in order to evaluate the biological relevance of literature-derived targets in the pathogenesis of diabetic neuropathy. Results SciMiner identified 1,026 ROS- and diabetes-related targets from the 1,154 biomedical papers (http://jdrf.neurology.med.umich.edu/ROSDiabetes/). Fifty-three targets were significantly over-represented in the ROS-diabetes literature compared to randomly selected literature. These over-represented targets included well-known members of the oxidative stress response including catalase, the NADPH oxidase family, and the superoxide dismutase family of proteins. Eight of the nine selected genes exhibited significant differential expression between diabetic and non-diabetic mice. For six genes, the direction of expression change in diabetes paralleled enhanced oxidative stress in the DRG. Conclusions Literature mining compiled ROS-diabetes related targets from the biomedical literature and led us to evaluate the biological relevance of selected targets in the pathogenesis of diabetic neuropathy.http://deepblue.lib.umich.edu/bitstream/2027.42/78315/1/1755-8794-3-49.xmlhttp://deepblue.lib.umich.edu/bitstream/2027.42/78315/2/1755-8794-3-49-S7.XLShttp://deepblue.lib.umich.edu/bitstream/2027.42/78315/3/1755-8794-3-49-S10.XLShttp://deepblue.lib.umich.edu/bitstream/2027.42/78315/4/1755-8794-3-49-S8.XLShttp://deepblue.lib.umich.edu/bitstream/2027.42/78315/5/1755-8794-3-49-S3.XLShttp://deepblue.lib.umich.edu/bitstream/2027.42/78315/6/1755-8794-3-49-S1.XLShttp://deepblue.lib.umich.edu/bitstream/2027.42/78315/7/1755-8794-3-49-S4.XLShttp://deepblue.lib.umich.edu/bitstream/2027.42/78315/8/1755-8794-3-49-S2.XLShttp://deepblue.lib.umich.edu/bitstream/2027.42/78315/9/1755-8794-3-49-S12.XLShttp://deepblue.lib.umich.edu/bitstream/2027.42/78315/10/1755-8794-3-49-S11.XLShttp://deepblue.lib.umich.edu/bitstream/2027.42/78315/11/1755-8794-3-49-S9.XLShttp://deepblue.lib.umich.edu/bitstream/2027.42/78315/12/1755-8794-3-49-S5.XLShttp://deepblue.lib.umich.edu/bitstream/2027.42/78315/13/1755-8794-3-49-S6.XLShttp://deepblue.lib.umich.edu/bitstream/2027.42/78315/14/1755-8794-3-49.pdfPeer Reviewe

Molecular insights of p47phox phosphorylation dynamics in the regulation of NADPH oxidase activation and superoxide production

Phagocyte superoxide production by a multicomponent NADPH oxidase is important in host defense against microbial invasion. However inappropriate NADPH oxidase activation causes inflammation. Endothelial cells express NADPH oxidase and endothelial oxidative stress due to prolonged NADPH oxidase activation predisposes many diseases. Discovering the mechanism of NADPH oxidase activation is essential for developing novel treatment of these diseases. The p47phox is a key regulatory subunit of NADPH oxidase; however, due to the lack of full protein structural information, the mechanistic insight of p47phox phosphorylation in NADPH oxidase activation remains incomplete. Based on crystal structures of three functional domains, we generated a computational structural model of the full p47phox protein. Using a combination of in silico phosphorylation, molecular dynamics simulation and protein/protein docking, we discovered that the C-terminal tail of p47phox is critical for stabilizing its autoinhibited structure. Ser-379 phosphorylation disrupts H-bonds that link the C-terminal tail to the autoinhibitory region (AIR) and the tandem Src homology 3 (SH3) domains, allowing the AIR to undergo phosphorylation to expose the SH3 pocket for p22phox binding. These findings were confirmed by site-directed mutagenesis and gene transfection of p47phox_/_ coronary microvascular cells. Compared with wild-type p47phoxcDNAtransfected cells, the single mutation of S379A completely blocked p47phox membrane translocation, binding to p22phox and endothelial O2 . production in response to acute stimulation of PKC. p47phox C-terminal tail plays a key role in stabilizing intramolecular interactions at rest. Ser-379 phosphorylation is a molecular switch which initiates p47phox conformational changes and NADPH oxidase-dependent superoxide production by cells

Prise de décisions de cadres confrontés à un environnement dynamique, coopératif et compétitif. Une approche en ergonomie cognitive (application à l'entraînement professionnel de handball de match)

Ce travail, adossé au paradigme de la prise de décision en situation (Klein, Orasanu, Calderwood, & Zsambok, 1993) porte sur le processus de prise de décisions d entraîneurs de handball au cours du match. La situation de match, caractérisée par ses aspects dynamiques, coopératifs et compétitifs, donne une conception chaotique de ce processus (Bowes & Jones, 2006). C est pourquoi, son étude nécessite de mobiliser plusieurs approches et modèles théoriques (e.g., cognition collective, théorie du focus régulateur [Higgins, 1997], théorie du contrôle [Carver & Scheier, 1982], modèles de persuasion [Chaiken, 1980 ; Petty & Cacioppo, 1986], communication dialogique [Goffman, 1961], modèle de l activité coopérative [Hoc, 2001]). Les données, issues de situations réelles de matchs (protocoles verbaux, choix de système défensif) ou d entretiens (e.g., auto-confrontations avec technique de rappel stimulé) font l objet d analyses quantitatives ou qualitatives. Les résultats révèlent les connaissances procédurales des entraîneurs concernant : la gestion de l équipe, les informations permettant aux joueurs d acquérir ou de mettre à jour des structures de connaissances adaptées à la situation, et les tentatives d influence des arbitres. Ces connaissances procédurales sont fonction des conditions du match (notamment le rapport d opposition entre les deux équipes) et influencées par des connaissances sur le jeu et les caractéristiques des joueurs. Différents modes de contrôle cognitif et styles de coaching sont aussi mis en évidence. Ainsi, le processus de prise de décisions d entraîneurs experts possède des régularités et suit des règles génériques, des heuristiques ou patterns.This work, based on naturalistic decision-making paradigm (Klein, Orasanu, Calderwood, & Zsambok, 1993) is focused on team sport (handball) coaches decision-making during match. The coaching activity is dynamic and chaotic (Bowes & Jones, 2006) and generated by on-going events, especially during match. Therefore, coaches decision-making studies require to use many theoretical approaches and models (e.g., team cognition, regulatory focus theory [Higgins, 1997], control theory [Carver & Scheier, 1982], persuasive models [Chaiken, 1980 ; Petty & Cacioppo, 1986], dialogic communication [Goffman, 1961], cooperative activity model [Hoc, 2001]). Data have been collected from official games (verbal protocols, defense system choices) or interviews (self-confrontation with stimulated technique recall) and analysed with quantitative and qualitative approach. Results highlight procedural knowledge concerning team management, information allowing players to acquire or update knowledge structures, and influence referee. This procedural knowledge varied depending on the game conditions and is influenced by the coach s deep knowledge of the game and player profiles. Results highlight also different cognitive control modes, and different coaching styles. There exist contextual factors effects on procedural knowledge and cognitive control modes. Among these factors, ratio of strength between the two teams appears especially salient. Therefore, our empirical studies show that expert coaches decision-making process have some regularity and follow generic rules, heuristics or patternsPARIS11-SCD-Bib. électronique (914719901) / SudocSudocFranceF