Shock pair observation

On day 84, 1969, the HEOS 1 satellite observed a shock pair connected with a plasma bulk velocity increase from 400 to approximately 750 km/sec. Both shocks were fast shocks. The forward shock had a Mach number of 1.7, the reverse shock had M(fast) = 1.4. The time interval between the two shocks was 7 hrs, 10 min. The time delay between HEOS 1 and Explorer 35 reverse shock observation (20 + or - 6 min) agrees with the computed time delay (11 + or - 4 min)

HEOS 1 helium observations in the solar wind

Results of alpha-particle observations performed by the European satellite HEOS 1, in the period from December 9, 1968, to April 13, 1969, and from September 6, 1969, to April 15, 1970, are presented. The average bulk velocities of protons V sub p and alpha-particles V sub alpha appear to be equal; however, due to an instrumental bias, the possibility of V sub alpha being lower than V sub p cannot be ruled out. Comparison with observations of Vela 3 and Explorer 34 satellites gives evidence of a dependence of helium abundance on the solar cycle. The problem of the stability of differences between the bulk velocities of protons and alpha-particles is investigated. The behavior of alpha-particles through interplanetary shock waves is illustrated in connection with magnetic field measurements

Large parallel and perpendicular electric fields on electron spatial scales in the terrestrial bow shock

Large parallel ($\leq$ 100 mV/m) and perpendicular ($\leq$ 600 mV/m) electric fields were measured in the Earth's bow shock by the vector electric field experiment on the Polar satellite. These are the first reported direct measurements of parallel electric fields in a collisionless shock. These fields exist on spatial scales comparable to or less than the electron skin depth (a few kilometers) and correspond to magnetic field-aligned potentials of tens of volts and perpendicular potentials up to a kilovolt. The perpendicular fields are amongst the largest ever measured in space, with energy densities of $\epsilon_0 E^2/ n k_b T_e$ of order 10%. The measured parallel electric field implies that the electrons can be demagnetized, which may result in stochastic (rather than coherent) electron heating

Thermal stability and aggregation of sulfolobus solfataricus b-glycosidase are dependent upon the N-e-methylation of specific lysyl residues: critical role of in vivo post-translational modifications.

Methylation in vivo is a post-translational modification observed in several organisms belonging to eucarya, bacteria, and archaea. Although important implications of this modification have been demonstrated in several eucaryotes, its biological role in hyperthermophilic archaea is far from being understood. The aim of this work is to clarify some effects of methylation on the properties of β-glycosidase from Sulfolobus solfataricus, by a structural comparison between the native, methylated protein and its unmethylated counterpart, recombinantly expressed in Escherichia coli. Analysis by Fourier transform infrared spectroscopy indicated similar secondary structure contents for the two forms of the protein. However, the study of temperature perturbation by Fourier transform infrared spectroscopy and turbidimetry evidenced denaturation and aggregation events more pronounced in recombinant than in native β-glycosidase. Red Nile fluorescence analysis revealed significant differences of surface hydrophobicity between the two forms of the protein. Unlike the native enzyme, which dissociated into SDS-resistant dimers upon exposure to the detergent, the recombinant enzyme partially dissociated into monomers. By electrospray mapping, the methylation sites of the native protein were identified. A computational analysis of β-glycosidase three-dimensional structure and comparisons with other proteins from S. solfataricus revealed analogies in the localization of methylation sites in terms of secondary structural elements and overall topology. These observations suggest a role for the methylation of lysyl residues, located in selected domains, in the thermal stabilization of β-glycosidase from S. solfataricu

Age-Related Impairment in Insulin Release The Essential Role of beta(2)-Adrenergic Receptor

In this study, we investigated the significance of β(2)-adrenergic receptor (β(2)AR) in age-related impaired insulin secretion and glucose homeostasis. We characterized the metabolic phenotype of β(2)AR-null C57Bl/6N mice (β(2)AR(-/-)) by performing in vivo and ex vivo experiments. In vitro assays in cultured INS-1E β-cells were carried out in order to clarify the mechanism by which β(2)AR deficiency affects glucose metabolism. Adult β(2)AR(-/-) mice featured glucose intolerance, and pancreatic islets isolated from these animals displayed impaired glucose-induced insulin release, accompanied by reduced expression of peroxisome proliferator-activated receptor (PPAR)γ, pancreatic duodenal homeobox-1 (PDX-1), and GLUT2. Adenovirus-mediated gene transfer of human β(2)AR rescued these defects. Consistent effects were evoked in vitro both upon β(2)AR knockdown and pharmacologic treatment. Interestingly, with aging, wild-type (β(2)AR(+/+)) littermates developed impaired insulin secretion and glucose tolerance. Moreover, islets from 20-month-old β(2)AR(+/+) mice exhibited reduced density of β(2)AR compared with those from younger animals, paralleled by decreased levels of PPARγ, PDX-1, and GLUT2. Overexpression of β(2)AR in aged mice rescued glucose intolerance and insulin release both in vivo and ex vivo, restoring PPARγ/PDX-1/GLUT2 levels. Our data indicate that reduced β(2)AR expression contributes to the age-related decline of glucose tolerance in mice

Three-body interactions in colloidal systems

We present the first direct measurement of three-body interactions in a colloidal system comprised of three charged colloidal particles. Two of the particles have been confined by means of a scanned laser tweezers to a line-shaped optical trap where they diffused due to thermal fluctuations. Upon the approach of a third particle, attractive three-body interactions have been observed. The results are in qualitative agreement with additionally performed nonlinear Poissson-Boltzmann calculations, which also allow us to investigate the microionic density distributions in the neighborhood of the interacting colloidal particles

Jeans analysis of self-gravitating systems in f(R)-gravity

Dynamics and collapse of collisionless self-gravitating systems is described by the coupled collisionless Boltzmann and Poisson equations derived from $f(R)$-gravity in the weak field approximation. Specifically, we describe a system at equilibrium by a time-independent distribution function $f_0(x,v)$ and two potentials $\Phi_0(x)$ and $\Psi_0(x)$ solutions of the modified Poisson and collisionless Boltzmann equations. Considering a small perturbation from the equilibrium and linearizing the field equations, it can be obtained a dispersion relation. A dispersion equation is achieved for neutral dust-particle systems where a generalized Jeans wave-number is obtained. This analysis gives rise to unstable modes not present in the standard Jeans analysis (derived assuming Newtonian gravity as weak filed limit of $f(R)=R$). In this perspective, we discuss several self-gravitating astrophysical systems whose dynamics could be fully addressed in the framework of $f(R)$-gravity.Comment: 8 pages, 2 figures, Accepted for publication in PR