A DNA-priming protein-boosting regimen significantly improves type 1 immune response but not protective immunity to Trypanosoma cruzi infection in a highly susceptible mouse strain

BALB/c or C57Bl/6 mice immunized with plasmids containing Trypanosoma cruzi genes developed specific immune responses and protective immunity against lethal parasitic infection. in contrast, in the highly susceptible mouse strain A/Sn, DNA vaccination reduced the peak parasitemia but promoted limited mouse survival after challenge. in the present study, we tested whether the immunogenicity and protective efficacy of vaccination could be improved by combining DNA and recombinant protein immunization regimens. A/Sn mice immunized with plasmid p154/13 which harbours the gene encoding Trypanosoma cruzi trans -sialidase developed a predominant type 1 immune response. in contrast, immunization with the recombinant Trypanosoma cruzi trans -sialidase protein adsorbed to alum generated a typical type 2 immune response. Simultaneous administration of both p154/13 and recombinant Trypanosoma cruzi trans -sialidase protein also led to a predominant type 2 immune response. Sequential immunization consisting of two priming doses of p154/13 followed by booster injections with recombinant Trypanosoma cruzi trans -sialidase protein significantly improved specific type 1 immune response, as revealed by a drastic reduction of the serum IgG1/IgG2a ratio and by an increase in the in vitro interferon-gamma secretion by CD4 T cells. Our observations confirm and extend previous data showing that a DNA-priming protein-boosting regimen might be a general strategy to enhance type 1 immune response to DNA vaccines. Upon challenge with Trypanosoma cruzi , no improvement in protective immunity was observed in mice immunized with the DNA-priming protein-boosting regimen when compared to animals that received DNA only. Therefore, our results suggest that in this experimental model there is no correlation between the magnitude of type 1 immune response and protective immunity against Trypanosoma cruzi infection.Universidade Federal de São Paulo, Escola Paulista Med, Ctr Terapia Genica Cintergen, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Ctr Terapia Genica Cintergen, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, BrazilWeb of Scienc

Structure of isobaric analog states in 91Nb populated by the 90Zr(a,t) reaction

Decay via proton emission of isobaric analog states (IAS's) in $^{91}{Nb}$ was studied using the $^{90}{Zr}(\alpha,t)$ reaction at $E_\alpha$=180 MeV. This study provides information about the damping mechanism of these states. Decay to the ground state and low-lying phonon states in $^{90}{Zr}$ was observed. The experimental data are compared with theoretical predictions wherein the IAS `single-particle' proton escape widths are calculated in a continuum RPA approach. The branching ratios for decay to the phonon states are explained using a simple model.Comment: 3 figures. submitted to Phys. Lett.

Fatores de produção na cultura do milho sob efeito de doses e queima de resíduos orgânicos.

Os resíduos orgânicos quando corretamente manejados e utilizados revertem-se em fornecedores de nutrientes e condicionadores das propriedades físicas e biológicas do solo, garantindo ambiente adequado para o desenvolvimento do sistema radicular das plantas por uma lado e aumentando a produção de alimentos, fibras e energia, por outro. O aproveitamento agrícola dos resíduos de podas das árvores das cidades, na forma de composto orgânico ou in natura tem sido apontado como uma solução adequada, uma vez que, proporciona a liberação de nutrientes para as plantas semcausar grande impacto..

Gamow-Teller Strengths of the Inverse-Beta Transition 176Yb --> 176Lu for Spectroscopy of Proton-Proton and other sub-MeV Solar Neutrinos

Discrete Gamow-Teller (GT) transitions, 176Yb-->176Lu at low excitation energies have been measured via the (3He,t) reaction at 450 MeV and at 0 degrees. For 176Yb, two low-lying states are observed, setting low thresholds Q(neutrino)=301 and 445 keV for neutrino capture. Capture rates estimated from the measured GT strengths, the simple two-state excitation structure, and the low Q(neutrino) in Yb--Lu indicate that Yb-based neutrino-detectors are well suited for a direct measurement of the complete sub-MeV solar electron-neutrino spectrum (including pp neutrinos) where definitive effects of flavor conversion are expected

Near-threshold Lambda(1520) production by the gamma p -> K+Lambda(1520) reaction at forward K+ angles

Differential cross sections and photon-beam asymmetries for the gamma p -> K+ Lambda(1520) reaction have been measured with linearly polarized photon beams at energies from the threshold to 2.4 GeV at 0.6<cos(theta)<1. A new bump structure was found at W=2.11 GeV in the cross sections. The bump is not well reproduced by theoretical calculations introducing a nucleon resonance with J<=3/2. This result suggests that the bump might be produced by a nucleon resonance possibly with J>=5/2 or by a new reaction process, for example an interference effect with the phi photoproduction having a similar bump structure in the cross sections.Comment: 5 pages, 4 figures, published in Phys. Rev. Let

The Renormalization Group and Singular Perturbations: Multiple-Scales, Boundary Layers and Reductive Perturbation Theory

Perturbative renormalization group theory is developed as a unified tool for global asymptotic analysis. With numerous examples, we illustrate its application to ordinary differential equation problems involving multiple scales, boundary layers with technically difficult asymptotic matching, and WKB analysis. In contrast to conventional methods, the renormalization group approach requires neither {\it ad hoc\/} assumptions about the structure of perturbation series nor the use of asymptotic matching. Our renormalization group approach provides approximate solutions which are practically superior to those obtained conventionally, although the latter can be reproduced, if desired, by appropriate expansion of the renormalization group approximant. We show that the renormalization group equation may be interpreted as an amplitude equation, and from this point of view develop reductive perturbation theory for partial differential equations describing spatially-extended systems near bifurcation points, deriving both amplitude equations and the center manifold.Comment: 44 pages, 2 Postscript figures, macro \uiucmac.tex available at macro archives or at ftp://gijoe.mrl.uiuc.edu/pu

Efeito de doses e queima de resíduos orgânicos sobre a biomassa microbiana do solo.

O carbono da biomassa microbiana é um indicador sensível às mudanças ocorridas no solo. Este trabalho teve o objetivo de avaliar o efeito da queima de resíduos orgânicos sobre o conteúdo de carbono da biomassa microbiana do solo (C-bio) cultivado com milho adubado e sem adubação química..

Interference effect between ϕ\phi and Λ(1520)\Lambda(1520) production channels in the γp→K+K−p\gamma p \rightarrow K^+K^-p reaction near threshold

The $\phi$-$\Lambda(1520)$ interference effect in the $\gamma p\to K^+K^-p$ reaction has been measured for the first time in the energy range from 1.673 to 2.173 GeV. The relative phases between $\phi$ and $\Lambda(1520)$ production amplitudes were obtained in the kinematic region where the two resonances overlap. The measurement results support strong constructive interference when $K^+K^-$ pairs are observed at forward angles, but destructive interference for proton emission at forward angles. Furthermore, the observed interference effect does not account for the $\sqrt{s}=2.1$ GeV bump structure in forward differential cross sections for $\phi$ photoproduction. This fact suggests possible exotic structures such as a hidden-strangeness pentaquark state, a new Pomeron exchange and rescattering processes via other hyperon states

Standing sausage waves in photospheric magnetic waveguides

By focusing on the oscillations of the cross-sectional area and the intensity of magnetic waveguides located in the lower solar atmosphere, we aim to detect and identify magnetohydrodynamic (MHD) sausage waves. Capturing several series of high-resolution images of pores and sunspots and employing wavelet analysis in conjunction with empirical mode decomposition (EMD) makes the MHD wave analysis possible. For this paper, two sunspots and one pore (with a light bridge) were chosen as representative examples of MHD waveguides in the lower solar atmosphere. The sunspots and pore display a range of periods from 4 to 65 minutes. The sunspots support longer periods than the pore - generally enabling a doubling or quadrupling of the maximum pore oscillatory period. All of these structures display area oscillations indicative of MHD sausage modes and in-phase behaviour between the area and intensity, presenting mounting evidence for the presence of the slow sausage mode within these waveguides. The presence of fast and slow MHD sausage waves has been detected in three different magnetic waveguides in the lower solar photosphere. Furthermore, these oscillations are potentially standing harmonics supported in the waveguides which are sandwiched vertically between the temperature minimum in the lower solar atmosphere and the transition region. Standing harmonic oscillations, by means of solar magneto-seismology, may allow insight into the sub-resolution structure of photospheric MHD waveguides

Wave Damping Observed in Upwardly Propagating Sausage-mode Oscillations contained within a Magnetic Pore

We present observational evidence of compressible MHD wave modes propagating from the solar photosphere through to the base of the transition region in a solar magnetic pore. High cadence images were obtained simultaneously across four wavelength bands using the Dunn Solar Telescope. Employing Fourier and wavelet techniques, sausage-mode oscillations displaying significant power were detected in both intensity and area fluctuations. The intensity and area fluctuations exhibit a range of periods from 181 to 412 s, with an average period ~290 s, consistent with the global p-mode spectrum. Intensity and area oscillations present in adjacent bandpasses were found to be out of phase with one another, displaying phase angles of 6fdg12, 5fdg82, and 15fdg97 between the 4170 Å continuum–G-band, G-band–Na i D1, and Na i D1–Ca ii K heights, respectively, reiterating the presence of upwardly propagating sausage-mode waves. A phase relationship of ~0° between same-bandpass emission and area perturbations of the pore best categorizes the waves as belonging to the "slow" regime of a dispersion diagram. Theoretical calculations reveal that the waves are surface modes, with initial photospheric energies in excess of 35,000 W m−2. The wave energetics indicate a substantial decrease in energy with atmospheric height, confirming that magnetic pores are able to transport waves that exhibit appreciable energy damping, which may release considerable energy into the local chromospheric plasma