LIM domain protein FHL1B interacts with PP2A catalytic β subunit - A novel cell cycle regulatory pathway

Four-and-a-half LIM domain protein 1B (FHL1B) is an alternatively-spliced isoform of FHL1. In this study, FHL1B was demonstrated to interact with the β catalytic subunit (Cβ) of a type 2A protein phosphatase (PP2A) by yeast two-hybrid screening. Domain studies using a small-scale yeast two-hybrid interaction assay revealed the mediation of protein-protein interaction by FHL1B's C-terminus. Interaction between FHL1B and PP2A was further verified by co-immunoprecipitation. FHL1B was also shown to shuttle between nucleus and cytoplasm at different phases of the cell cycle. These data suggest that the FHL1B/PP2A Cβ interaction may illustrate a novel cell-cycle regulatory pathway. Structured summary: MINT- 8044739: FHL1B (uniprotkb: Q13642-2) physically interacts (MI: 0915) with PP2Acbeta (uniprotkb: P62714) by two hybrid (MI: 0018). MINT- 8044769, MINT- 8044778: FHL1B (uniprotkb: Q13642-2) physically interacts (MI: 0915) with PP2Acbeta (uniprotkb: P62714) by anti bait coimmunoprecipitation (MI: 0006). © 2010 Federation of European Biochemical Societies.link_to_subscribed_fulltex