High velocity blue-shifted FeII absorption in the dwarf star-forming galaxy PHL293B: Evidence for a wind driven supershell?

X-shooter and ISIS WHT spectra of the starforming galaxy PHL 293B also known as A2228-00 and SDSS J223036.79-000636.9 are presented in this paper. We find broad (FWHM = 1000km/s) and very broad (FWZI = 4000km/s) components in the Balmer lines, narrow absorption components in the Balmer series blueshifted by 800km/s, previously undetected FeII multiplet (42) absorptions also blueshifted by 800km/s, IR CaII triplet stellar absorptions consistent with [Fe/H] < -2.0 and no broad components or blushifted absorptions in the HeI lines. Based on historical records, we found no optical variability at the 5 sigma level of 0.02 mag between 2005 and 2013 and no optical variability at the level of 0.1mag for the past 24 years. The lack of variability rules out transient phenomena like luminous blue variables or SN IIn as the origin of the blue shifted absorptions of HI and FeII. The evidence points to either a young and dense expanding supershell or a stationary cooling wind, in both cases driven by the young cluster wind.Comment: Accepted for publication in MNRAS; 15 pages, 10 figure

p38(MAPK)/p53 signalling axis mediates neuronal apoptosis in response to tetrahydrobiopterin-induced oxidative stress and glucose uptake inhibition: implication for neurodegeneration.

BH4 (tetrahydrobiopterin) induces neuronal demise via production of ROS (reactive oxygen species). In the present study we investigated the mechanisms of its toxicity and the redox signalling events responsible for the apoptotic commitment in SH-SY5Y neuroblastoma cells and in mouse primary cortical neurons. We identified in p38(MAPK)/p53 a BH4-responsive pro-apoptotic signalling axis, as demonstrated by the recovery of neuronal viability achieved by gene silencing or pharmacological inhibition of both p38(MAPK) and p53. BH4-induced oxidative stress was characterized by a decrease in the GSH/GSSG ratio, an increase in protein carbonylation and DNA damage. BH4 toxicity and the redox-activated apoptotic pathway were counteracted by the H2O2-scavengers catalase and N-acetylcysteine and enhanced by the GSH neo-synthesis inhibitor BSO (buthionine sulfoximine). We also demonstrated that BH4 impairs glucose uptake and utilization, which was prevented by catalase administration. This effect contributes to the neuronal demise, exacerbating BH4-induced nuclear damage and the activation of the pro-apoptotic p38(MAPK)/p53 axis. Inhibition of glucose uptake was also observed upon treatment with 6-hydroxydopamine, another redox-cycling molecule, suggesting a common mechanism of action for auto-oxidizable neurotoxins

Sulphur abundance determinations in star-forming regions-I: Ionization Correction Factor

In the present work we used a grid of photoionization models combined with stellar population synthesis models to derive reliable Ionization Correction Factors (ICFs) for the sulphur in star-forming regions. These models cover a large range of nebular parameters and yielding ionic abundances in consonance with those derived through optical and infrared observational data of star-forming regions. From our theoretical ICFs, we suggested an {\alpha} value of 3.27 in the classical Stasinska formulae. We compared the total sulphur abundance in the gas phase of a large sample of objects by using our Theoretical ICF and other approaches. In average, the differences between the determinations via the use of the different ICFs considered are similar to the uncertainties in the S/H estimations. Nevertheless, we noted that for some objects it could reach up to about 0.3 dex for the low metallicity regime. Despite of the large scatter of the points, we found a trend of S/O ratio to decrease with the metallicity, independently of the ICF used to compute the sulphur total abundance.Comment: Accepted for publication in MNRAS, 21 pages, 8 figures, 5 table

Interaction effects on galaxy pairs with Gemini/GMOS- II: Oxygen abundance gradients

In this paper we derived oxygen abundance gradients from HII regions located in eleven galaxies in eight systems of close pairs. Long-slit spectra in the range 4400-7300A were obtained with the Gemini Multi-Object Spec- trograph at Gemini South (GMOS). Spatial profiles of oxygen abundance in the gaseous phase along galaxy disks were obtained using calibrations based on strong emission-lines (N2 and O3N2). We found oxygen gradients signifi- cantly flatter for all the studied galaxies than those in typical isolated spiral galaxies. Four objects in our sample, AM1219A, AM1256B, AM 2030A and AM2030B, show a clear break in the oxygen abundance at galactocentric radius R/R25 between 0.2 and 0.5. For AM1219A and AM1256B we found negative slopes for the inner gradients, and for AM2030B we found a positive one. In all these three cases they show a flatter behaviour to the outskirts of the galaxies. For AM2030A, we found a positive-slope outer gradient while the inner one is almost compatible with a flat behaviour. A decrease of star forma- tion efficiency in the zone that corresponds to the oxygen abundance gradient break for AM1219A and AM2030B was found. For the former, a minimum in the estimated metallicities was found very close to the break zone that could be associated with a corotation radius. On the other hand, AM1256B and AM2030A, present a SFR maximum but not an extreme oxygen abundance value. All the four interacting systems that show oxygen gradient breakes the extreme SFR values are located very close to break zones. Hii regions lo- cated in close pairs of galaxies follow the same relation between the ionization parameter and the oxygen abundance as those regions in isolated galaxies.Comment: 30 pages, 14 figures, accepted MNRAS, (Figs. 1 and 2 are in low resolution

Carcinoma cells activate AMP-activated protein kinase-dependent autophagy as survival response to kaempferol-mediated energetic impairment.

Kaempferol, a dietary cancer chemopreventive polyphenol, has been reported to trigger apoptosis in several tumor histotypes, but the mechanism underlying this phenomenon is not fully understood. Here, we demonstrate that in HeLa cells, kaempferol induces energetic failure due to inhibition of both glucose uptake and Complex I of the mitochondrial respiratory chain. As adaptive response, cells activate autophagy, the occurrence of which was established cytofluorometrically, upon acridine orange staining, and immunochemically, by following the increase of the autolysosome-associated form of the microtubule-associated protein light chain 3 (LC3-II). Autophagy is an early and reversible process occurring as survival mechanisms against apoptosis. Indeed, chemical inhibition of autophagy, by incubations with monensin, wortmannin, 3-methyladenine, or by silencing Atg5, significantly increases the extent of apoptosis, which takes place via the mitochondrial pathway, and shortens the time in which the apoptotic markers are detectable. We also demonstrate that autophagy depends on the early activation of the AMP-activated protein kinase (AMPK)/mTOR-mediated pathway. The overexpression of dominant negative AMPK results in a decrease of autophagic cells, a decrement of LC3-II levels, and a significant increase of apoptosis. Experiments performed with another carcinoma cell line yielded the same results, suggesting for kaempferol a unique mechanism of action

Under the ROS…thiol network is the principal suspect for autophagy commitment.

Low molecular weight and protein sulphydryls undergo reactive oxygen species (ROS)-mediated oxidation. However, in contrast to the irreversible damages that oxidative conditions yield on biomolecules, the oxidation of reactive cysteines frequently results in reversible modifications, which represent the prototype of the molecular mechanisms underlying redox signaling. Many proteins involved in a wide range of cellular processes have been classified as “redoxsensitive,” thereby modulating their function/activity dependent on the redox state of their critical thiols. Growing evidence from the past few years supports the idea that ROS production also correlates with the occurrence of autophagy. Nonetheless, the cysteine protease Atg4 remains the sole example of a protein whose redox regulation has been completely characterized and demonstrated to be necessary for the progression of autophagy. The principal aim of this commentary is to draw attention to the remarkable number of proteins that can fit the double role of: (i) being involved in autophagy, especially in autophagosome formation and (ii) sensing alterations of the cellular redox state by means of reactive cysteine residues. We will also attempt to provide a hypothetical model to explain the possible functional role of thiols in the occurrence of autophagy and outline a network of redox reactions likely concurring to allow the correct initiation and completion of autophagosomes

Rapid onset of bronchodilation with formoterol/beclomethasone Modulite and formoterol/budesonide Turbuhaler as compared to formoterol alone in patients with COPD

In the present study, we examined whether there is a difference in the onset of bronchodilatation between formoterol/beclomethasone 12/200 μg Modulite and formoterol/budesonide 9/320 μg Turbuhaler in patients with COPD. We enrolled 28 patients with stable COPD. Both formoterol/beclomethasone and formoterol/budesonide elicited a larger mean FEV1–AUC0−15min than formoterol alone, whereas there was no significant difference between their FEV1–AUC0−15min. Also the change in FEV1 15 min after inhalation of formoterol/beclomethasone combination or formoterol/budesonide combination was greater than that induced by formoterol alone. This study confirms the rapid effect of the inhaled corticosteroid component when combined with formoterol and indicates that the onset of bronchodilation of formoterol/beclomethasone Modulite and formoterol/budesonide Turbuhaler are similar and greater than formoterol alone in patients with COPD

Glutamine deprivation enhances antitumor activity of 3-bromopyruvate through the stabilization of monocarboxylate transporter-1

Anticancer drug efficacy might be leveraged by strategies to target certain biochemical adaptations of tumors. Here we show how depriving cancer cells of glutamine can enhance the anticancer properties of 3-bromopyruvate, a halogenated analog of pyruvic acid. Glutamine deprival potentiated 3-bromopyruvate chemotherapy by increasing the stability of the monocarboxylate transporter-1, an effect that sensitized cells to metabolic oxidative stress and autophagic cell death. We further elucidated mechanisms through which resistance to chemopotentiation by glutamine deprival could be circumvented. Overall, our findings offer a preclinical proof-of-concept for how to employ 3-bromopyruvate or other monocarboxylic-based drugs to sensitize tumors to chemotherap

Interaction effects on galaxy pairs with Gemini/GMOS- I: electron density

We present an observational study about the impacts of the interactions on the electron density of HII regions located in seven systems of interacting galaxies. The data consist of long-slit spectra in the range 4400-7300 A, obtained with the Gemini Multi-Object Spectrograph at Gemini South (GMOS-S). The electron density was determined using the ratio of emission-lines [S II] lambda 6716/ lambda 6731. Our results indicate that the electron density estimates obtained of HII regions from our sample of interacting galaxies are systematically higher than those derived for isolated galaxies. The mean electron density values of interacting galaxies are in the range of Ne = 24 - 532 cm^{-3}, while those obtained for isolated galaxies are in the range of Ne = 40 - 137 cm^{-3}. Comparing the observed emission lines with predictions of photoionization models, we verified that almost all the HII regions of the galaxies AM1054A, AM2058B, AM2306B have emission lines excited by shocks, such as in AM2322B (one point) and AM 2322A (four points). No correlation is obtained between the presence of shocks and electron densities. Indeed, the highest electron density values found in our sample do not belong to the objects with gas shock excitation. We emphasize the importance of considering theses quantities especially when the metallicity is derived for this type of systems.Fil: Krabbe, A.. Universidade Do Vale Do Paraiba; BrasilFil: Rosa, D. A.. Universidade Do Vale Do Paraiba; BrasilFil: Dors Jr., Oli L.. Universidade Do Vale Do Paraiba; BrasilFil: Pastoriza, Miriani G.. Universidade Federal Do Rio Grande Do Sul; BrasilFil: Winge, C.. Gemini Observatory; ChileFil: Hägele, Guillermo Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Astrofísica de la Plata; Argentina; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Astronómicas y Geofísicas; ArgentinaFil: Cardaci, Monica Viviana. Universidad Nacional de la Plata. Facultad de Ciencias Astronómicas y Geofísicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Astrofísica de La Plata; Argentina; ArgentinaFil: Rodrigues, Irapuan. Universidade Do Vale Do Paraiba; Brasi