Viscoelastic gels of guar and xanthan gum mixtures provide long-term stabilization of iron micro- and nanoparticles

Iron micro- and nanoparticles used for groundwater remediation and medical applications are prone to fast aggregation and sedimentation. Diluted single biopolymer water solutions of guar gum (GG) or xanthan gum (XG) can stabilize these particles for few hours providing steric repulsion and by increasing the viscosity of the suspension. The goal of the study is to demonstrate that amending GG solutions with small amounts of XG (XG/GG weight ratio 1:19; 3 g/L of total biopolymer concentration) can significantly improve the capability of the biopolymer to stabilize highly concentrated iron micro- and nanoparticle suspensions. The synergistic effect between GG and XG generates a viscoelastic gel that can maintain 20 g/L iron particles suspended for over 24 h. This is attributed to (i) an increase in the static viscosity, (ii) a combined polymer structure the yield stress of which contrasts the downward stress exerted by the iron particles, and (iii) the adsorption of the polymers to the iron surface having an anchoring effect on the particles. The XG/GG viscoelastic gel is characterized by a marked shear thinning behavior. This property, coupled with the low biopolymer concentration, determines small viscosity values at high shear rates, facilitating the injection in porous media. Furthermore, the thermosensitivity of the soft elastic polymeric network promotes higher stability and longer storage times at low temperatures and rapid decrease of viscosity at higher temperatures. This feature can be exploited in order to improve the flowability and the delivery of the suspensions to the target as well as to effectively tune and control the release of the iron particle

The Transcription Factor GLI1 Mediates TGFb1 Driven EMT in Hepatocellular Carcinoma via a SNAI1-Dependent Mechanism

The role of the epithelial-to-mesenchymal transition (EMT) during hepatocellular carcinoma (HCC) progression is well established, however the regulatory mechanisms modulating this phenomenon remain unclear. Here, we demonstrate that transcription factor glioma-associated oncogene 1 (GLI1) modulates EMT through direct up-regulation of SNAI1 and serves as a downstream effector of the transforming growth factor-b1 (TGFb1) pathway, a well-known regulator of EMT in cancer cells. Overexpression of GLI1 increased proliferation, viability, migration, invasion, and colony formation by HCC cells. Conversely, GLI1 knockdown led to a decrease in all the above-mentioned cancer-associated phenotypes in HCC cells. Further analysis of GLI1 regulated cellular functions showed that this transcription factor is able to induce EMT and identified SNAI1 as a transcriptional target of GLI1 mediating this cellular effect in HCC cells. Moreover, we demonstrated that an intact GLI1-SNAI1 axis is required by TGFb1 to induce EMT in these cells. Together, these findings define a novel cellular mechanism regulated by GLI1, which controls the growth and EMT phenotype in HCC.National Institutes of Health Grants CA100882 and CA128633 (to LRR) and CA165076; the Mayo Clinic Center for Cell Signaling in Gastroenterology (NIDDK P30DK084567) (to MEFZ); the Mayo Clinic Cancer Center (CA15083), the Mayo Clinic Center for Translational Science Activities (NIH/NCRR CTSA Grant Number KL2 RR024151), and an American Gastroenterological Association Foundation for Digestive Health and Nutrition Bridging Grant (to LRR)

Energy performance and climate control in mechanically ventilated greenhouses: A dynamic modelling-based assessment and investigation

Controlled environment agriculture in greenhouse is a promising solution for meeting the increasing food demand of world population. The accurate control of the indoor environmental conditions proper of greenhouses enhances high crop productivity but, contemporarily, it entails considerable energy consumption due to the adoption of mechanical systems. This work presents a new modelling approach for estimating the energy consumption for climate control of mechanically ventilated greenhouses. The novelty of the proposed energy model lies in its integrated approach in simulating the greenhouse dynamics, considering the dynamic thermal and hygric behaviour of the building and the dynamic response of the cultivated crops to the variation of the solar radiation. The presented model simulates the operation of the systems and the energy performance, considering also the variable angular speed fans that are a new promising energy-efficient technology for this productive sector. The main outputs of the model are the hourly thermal and electrical energy use for climate control and the main indoor environmental conditions. The presented modelling approach was validated against a dataset acquired in a case study of a new fully mechanically controlled greenhouse during a long-term monitoring campaign. The present work contributes to increase the knowledge about the dynamics and the energy consumption of greenhouses, and it can be a valuable decision support tool for industry, farmers, and researchers to properly address an energy efficiency optimisation in mechanically ventilated greenhouses to reach the overall objective of decreasing the rising energy consumption of the agricultural sector

Nueva nave de Ensayos Mecánicos en el Instituto Eduardo Torroja

This important building meets an urgent need at the Institute Eduardo Torroja to have a mechanical testing hall that is capable of dealing with all kinds of tests that are likely to arise in the foreseeable future. In designing it account has been taken of all requirements anticipated as a result of the experience obtained during the use of the earlier testing hall which the Institute already has. The new testing hall consists essentially of a metallic structure, 33.50 m high, 11.50 m wide and 8 m high, enclosed by prefabricated LECA type panels. The hall has a powerful reinforced concrete slab, 11.50 m wide and 25.50 m long, which is 1.20 m thick. The slab has 270 anchorage points, with a load capacity of 50 t and 100 t each. These points can be loaded singly, or in groups. The slab rests on two lateral reinforced concrete walls, of 80 cm thickness. Below the slab there is a basement, which can be used in conjunction with the hall above for testing purposes, since the anchorage points make it possible to communicate both sides of the slab. A careful system of illumination has also been provided, and heavy vehicles can enter both the basement and the main hall. One ten ton bridge crane has been installed, although two such cranes can be fitted. All calculations have been done with the aid of the electronic computer of the I.E.T.c.c. The total design makes this project one of the most important of its type in Europe.A los quince años de estrenar la primera nave de Ensayos Mecánicos con que contó el Instituto, se inaugura esta segunda nave. A ella nos vamos a referir en el presente artículo, esencialmente descriptivo. No obstante, son tan singulares las razones que han motivado su necesidad que, aunque sea en forma sumamente breve, creemos de interés dar cuenta de ellas en este preámbulo

External gap progression after cyclic fatigue of adhesive overlays and crowns made with high translucency zirconia or lithium silicate

Objectives: To evaluate three-dimensional external gap progression after chewing simulation of high translucency zirconia (HTZ) and zirconia-reinforced lithium silicate (ZLS) applied on endodontically treated teeth with different preparation designs. Materials and Method: Endodontically treated molars were prepared with low-retentive (adhesive overlay) and high-retentive (full crown) designs above cementum-enamel junction and restored with HTZ and ZLS. Micro-computed tomography analysis was assessed before and after chewing simulation to evaluate three-dimensionally the external gap progression. Results were statistically analyzed with two-way ANOVA and post-hoc Tukey test. Results: High-retentive preparation design had a significantly inferior gap progression compared to the overlay preparation (p < 0.01); ZLS exhibited a significant inferior gap progression compared to HTZ (p < 0.01). Conclusions: High-retentive preparations restored with ZLS seem to better perform in maintaining the sealing of the external margin after cyclic fatigue. Clinical significance: The clinician should pay attention to the proper combination of preparation designs and ceramic material selection for an endodontically treated molar restoration. HTZ seems to perform worse than lithium silicate in terms of marginal sealing, still showing lacks in resistance to cyclic fatigue when adhesive preparations are performed