Cysteamine Suppresses Invasion, Metastasis and Prolongs Survival by Inhibiting Matrix Metalloproteinases in a Mouse Model of Human Pancreatic Cancer

Background: Cysteamine, an anti-oxidant aminothiol, is the treatment of choice for nephropathic cystinosis, a rare lysosomal storage disease. Cysteamine is a chemo-sensitization and radioprotection agent and its antitumor effects have been investigated in various tumor cell lines and chemical induced carcinogenesis. Here, we investigated whether cysteamine has anti-tumor and anti-metastatic effects in transplantable human pancreatic cancer, an aggressive metastatic disease. Methodology/Principal Findings: Cysteamine’s anti-invasion effects were studied by matrigel invasion and cell migration assays in 10 pancreatic cancer cell lines. To study mechanism of action, we examined cell viability and matrix metalloproteinases (MMPs) activity in the cysteamine-treated cells. We also examined cysteamine’s anti-metastasis effect in two orthotopic murine models of human pancreatic cancer by measuring peritoneal metastasis and survival of animals. Cysteamine inhibited both migration and invasion of all ten pancreatic cancer cell lines at concentrations (,25 mM) that caused no toxicity to cells. It significantly decreased MMPs activity (IC50 38–460 mM) and zymographic gelatinase activity in a dose dependent manner in vitro and in vivo; while mRNA and protein levels of MMP-9, MMP-12 and MMP-14 were slightly increased using the highest cysteamine concentration. In vivo, cysteamine significantly decreased metastasis in two established pancreatic tumor models, although it did not affect the size of primary tumors. Additionally, cysteamin

Self-radioiodination of iodogen

WOS: 000167611400006PubMed ID: 11258523Iodogen (1,3,4,6-tetrachloro-3 alpha ,6 alpha -diphenylglucoluril) is commonly used for the radioiodination of proteins as an oxidative agent. The oxidative character of iodogen is not clear, but the two carbonyl groups in its structure probably have an essential role in its oxidizing character. In this study, the self-radioiodination of iodogen has been examined. It was observed that about 10-20% of the initial iodine radioactivity was consumed for the self-radioiodination of iodogen itself. On the other hand, the radioiodinated iodogen removed by ethyl alcohol from the iodogen-coated tubes showed clearly that no thyroid uptake was observed and that it was rapidly cleared out from the whole body of a rabbit administered with the radioiodinated iodogen by injection via the ear vein. (C) 2001 Elsevier Science Ltd. All rights reserved

(18)FDG conjugated magnetic nanoparticle probes: synthesis and in vitro investigations on MCF-7 breast cancer cells

WOS: 000314895400026(18)FDG conjugated magnetic iron oxide nanoparticles (MNPs) were synthesized as PET-MR hybrid imaging agent. Synthesized and characterized NPs were then applied to MCF-7 human breast cancer cells. (18)FDG conjugated MNPs exhibited the cell incorporation ratio up to 30 %. As well as the characterization studies, apoptotic effects were observed depending on the cellular incorporations by the time. In conclusion, synthesized structures could have a potential as hybrid imaging agent in PET-MR imaging systems besides apoptotic effect on cancer cells.Ege University Scientific Research FundEge University [2008 NBE 08, 2009 FEN 007]The authors thank to Ege University Scientific Research Fund for the financial support with the Project Numbers 2008 NBE 08 and 2009 FEN 007. Authors also thank to Adnan Menderes University Science Technology Research and Application Center for cell culture experiments