Application of kaolin and italian natural chabasite-rich zeolitite to mitigate the effect of global warming in vitis vinifera l. Cv. sangiovese

High temperatures and the anomalous distribution of rainfall during the growing season may have a negative impact on grapevine yield and berry composition. In recent years, many studies have focused on the application of agronomical techniques to reduce the negative impact of heat waves on secondary metabolites such as phenols. In particular, treatments with kaolin have shown positive effects on reducing canopy temperatures, enhancing the accumulation of anthocyanins. In regard to the above, three treatments were evaluated: untreated control (C), kaolin (CAO), and chabasite-rich zeolitites (ZEO) applications on cv. Sangiovese in order to verify the cooling effects on leaves and bunches, and the impact on gas exchange, yield parameters, berry composition, and on both chemical and sensory notes of wine. Minerals were sprayed twice around the veraison on the entire canopy at a 3% concentration. The results showed that the application of the minerals was able to reduce the berry temperatures in both years of the trial as compared to the untreated control (C), without affecting vine gas exchange, yield, and soluble solid accumulation. Furthermore, the cooling effect determined an increase in anthocyanin on both the grapes and the wine. At testing, CAO and ZEO wines stood out regarding greater color intensity and were preferred by the judges

Innovative approach for a classic target: fragment screening on trypanothione reductase reveals new opportunities for drug design

Trypanothione reductase (TR) is a key factor in the redox homeostasis of trypanosomatid parasites, critical for survival in the hostile oxidative environment generated by the host to fight infection. TR is considered an attractive target for the development of new trypanocidal agents as it is essential for parasite survival but has no close homolog in humans. However, the high efficiency and turnover of TR challenging targets since only potent inhibitors, with nanomolar IC50, can significantly affect parasite redox state and viability. To aid the design of effective compounds targeting TR, we performed a fragment-based crystal screening at the Diamond Light Source XChem facility using a library optimized for follow-up synthesis steps. The experiment, allowing for testing over 300 compounds, resulted in the identification of 12 new ligands binding five different sites. Interestingly, the screening revealed the existence of an allosteric pocket close to the NADPH binding site, named the "doorstop pocket" since ligands binding at this site interfere with TR activity by hampering the "opening movement" needed to allow cofactor binding. The second remarkable site, known as the Z-site, identified by the screening, is located within the large trypanothione cavity but corresponds to a region not yet exploited for inhibition. The fragments binding to this site are close to each other and have some remarkable features making them ideal for follow-up optimization as a piperazine moiety in three out of five fragments

A Novel Workflow for Electrophysiology Studies in Patients with Brugada Syndrome

Brugada Syndrome (BrS) is a primary electrical epicardial disease characterized by ST-segment elevation followed by a negative T-wave in the right precordial leads on the surface electrocardiogram (ECG), also known as the ‘type 1’ ECG pattern. The risk stratification of asymptomatic individuals with spontaneous type 1 ECG pattern remains challenging. Clinical and electrocardiographic prognostic markers are known. As none of these predictors alone is highly reliable in terms of arrhythmic prognosis, several multi-factor risk scores have been proposed for this purpose. This article presents a new workflow for processing endocardial signals acquired with high-density RV electro-anatomical mapping (HDEAM) from BrS patients. The workflow, which relies solely on Matlab software, calculates various electrical parameters and creates multi-parametric maps of the right ventricle. The workflow, but it has already been employed in several research studies involving patients carried out by our group, showing its potential positive impact in clinical studies. Here, we will provide a technical description of its functionalities, along with the results obtained on a BrS patient who underwent an endocardial HDEAM

Impact of the environmental pollution on cardiovascular diseases: from epidemiological to molecular evidence

Environmental pollution poses a significant threat to human health, particularly concerning its impact on cardiovascular diseases (CVDs). This review synthesizes epidemiological and molecular evidence to elucidate the intricate relationship between environmental pollutants and CVDs. Epidemiological studies highlight the association between exposure to air, water, and soil pollutants and increased CVD risk, including hypertension, coronary artery disease, and stroke. Furthermore, molecular investigations unravel the underlying mechanisms linking pollutant exposure to CVD pathogenesis, such as oxidative stress, inflammation, endothelial dysfunction, and autonomic imbalance. Understanding these molecular pathways is crucial for developing targeted interventions and policy strategies to mitigate the adverse effects of environmental pollution on cardiovascular health. By integrating epidemiological and molecular evidence, this review provides insights into the complex interplay between environmental factors and CVDs, emphasizing the urgent need for comprehensive preventive measures and environmental policies to safeguard public health

A Performance Analysis of the Network Formation Process in IEEE 802.15.4e TSCH Wireless Sensor/Actuator Networks

Time Slotted Channel Hopping (TSCH) is one of the access behavior modes defined in the IEEE 802.15.4e standard. It combines time slotted access with multi-channel and channel hopping capabilities, providing predictable latency, energy efficiency, high network capacity, and high communication reliability. In this paper we focus on the formation process of TSCH networks, which relies on the regular advertisement of Enhanced Beacons (EBs). We consider a simple random-based advertisement algorithm, and evaluate its performance, through analysis and simulation, in terms of joining time (i.e., total time taken by a new node to join the network). We found that the joining time depends on a number of factors and, mainly, on the number of channels used for EB advertisement

The Prickle Effect Comes From Fabrics Made of South American Camelid (Alpaca and Lama) Fibers. Mechanical and/or Genetic Solutions.

In this paper we intends to analyze the physical attributes that determine the comfort of fabrics made of South American Camelid fibers (Lama and Alpaca), the effect on their value and their possible mechanical and/or genetic solutions. While emphasis has always been on mean fiber diameter, the fiber frequency exceeding 30 microns has a key role in quality. This is essential for light fabrics, where the effect of prickle plays a critical part in consumer´s choice. Yet the genetic solution of the problem lies in the slow selection response. Dehairing provides an immediate solution, though excessive fiber breakage should be addressed. It is concluded that the textile fiber quality of South American Camelids is promissory if the presence of objectionable fibers is solved, resulting in a tolerable frequency for consumers (<3%). This process could be explored via genetic selection or applying dehairing technology. This implies a true paradigm shift with regard to the classic textile processing of Alpaca and Lama fibers. This would enhance the fiber softness to touch, together with other important features that would render the fiber price more competitive