: Tubulin dimer binding proteins

International audienceMicrotubules play an essential role in eukaryotic cells, where they perform a wide variety of functions. In this paper, we describe the characterization of proteins associated to tubulin dimer in its native form, using affinity chromatography and mass spectrometry. We used an immunoaffinity column with coupled-monoclonal antibody directed against the alpha-tubulin C-terminus. Tubulin was first loaded onto the column, then interphase and mitotic cell lysates were chromatographed. Tubulin-binding proteins were eluted using a peptide mimicking the alpha-tubulin C-terminus. Elution fractions were analyzed by SDS-PAGE, and a total of 14 proteins were identified with high confidence by mass spectrometry. These proteins could be grouped in four classes: known tubulin-binding proteins, one microtubule-associated protein, heat shock proteins, and proteins that were not shown previously to bind tubulin dimer or microtubules

The alcohol use disorders identification test (AUDIT): validation of a Nepali version for the detection of alcohol use disorders and hazardous drinking in medical settings

BACKGROUND: Alcohol problems are a major health issue in Nepal and remain under diagnosed. Increase in consumption are due to many factors, including advertising, pricing and availability, but accurate information is lacking on the prevalence of current alcohol use disorders. The AUDIT (Alcohol Use Disorder Identification Test) questionnaire developed by WHO identifies individuals along the full spectrum of alcohol misuse and hence provides an opportunity for early intervention in non-specialty settings. This study aims to validate a Nepali version of AUDIT among patients attending a university hospital and assess the prevalence of alcohol use disorders along the full spectrum of alcohol misuse. METHODS: This cross-sectional study was conducted in patients attending the medicine out-patient department of a university hospital. DSM-IV diagnostic categories (alcohol abuse and alcohol dependence) were used as the gold standard to calculate the diagnostic parameters of the AUDIT. Hazardous drinking was defined as self reported consumption of ≥21 standard drink units per week for males and ≥14 standard drink units per week for females. RESULTS: A total of 1068 individuals successfully completed the study. According to DSM-IV, drinkers were classified as follows: No alcohol problem (n=562; 59.5%), alcohol abusers (n= 78; 8.3%) and alcohol dependent (n=304; 32.2%). The prevalence of hazardous drinker was 67.1%. The Nepali version of AUDIT is a reliable and valid screening tool to identify individuals with alcohol use disorders in the Nepalese population. AUDIT showed a good capacity to discriminate dependent patients (with AUDIT ≥11 for both the gender) and hazardous drinkers (with AUDIT ≥5 for males and ≥4 for females). For alcohol dependence/abuse the cut off values was ≥9 for both males and females. CONCLUSION: The AUDIT questionnaire is a good screening instrument for detecting alcohol use disorders in patients attending a university hospital. This study also reveals a very high prevalence of alcohol use disorders in Nepal

Effect of Training on Primary Care Residents' Performance in Brief Alcohol Intervention: A Randomized Controlled Trial

Background: Brief alcohol interventions (BAI) reduce alcohol use and related problems in primary care patients with hazardous drinking behavior. The effectiveness of teaching BAI on the performance of primary care residents has not been fully evaluated. Methods: A cluster randomized controlled trial was conducted with 26 primary care residents who were randomized to either an 8-hour, interactive BAI training workshop (intervention) or a lipid management workshop (control). During the 6-month period after training (i.e., from October 1, 2003 to March 30, 2004), 506 hazardous drinkers were identified in primary care, 260 of whom were included in the study. Patients were interviewed immediately and then 3months after meeting with each resident to evaluate their perceptions of the BAI experience and to document drinking patterns. Results: Patients reported that BAI trained residents: conducted more components of BAI than did controls (2.4 vs 1.5, p = .001); were more likely to explain safe drinking limits (27% vs 10%, p = .001) and provide feedback on patients' alcohol use (33% vs 21%, p = .03); and more often sought patient opinions on drinking limits (19% vs 6%, p = .02). No between-group differences were observed in patient drinking patterns or in use of 9 of the 12 BAI components. Conclusions: The BAI-trained residents did not put a majority of BAI components into practice, thus it is difficult to evaluate the influence of BAI on the reduction of alcohol use among hazardous drinker

ASPECTS OF THE BIRD FAUNA FROM CIRIC VALLEY’S (IAȘI)

The Ciric Valley, which is located in the northeastern region of Iași, integrates a considerable variety of habitats. Wetlands are dominant, being partially covered with compact reed beds. The other habitats are the deciduous forest, and meadows located in the northern part of the studied area. These allow the settlement of a diverse bird fauna in the area. Using the method of transects, the fixed-point observation, and sound identification, we identified 84 bird species between March 2020 and February 2021. Most species belong to the order Passeriformes (53 species), the rest of the orders having a significantly lower diversity. With the exception of the 20 sedentary species, Ciric’s avifauna consisted mostly of migratory species (39 summer visitors, 8 winter visitors, 13 vagrant species), and 5 partial migrant species during the research period. Among the identified bird species are 58 breeding species and 5 possible breeding species, representing 75% of the total number of the observed bird species. During the study, we identified 2 vulnerable species (Nycticorax nycticorax - black-crowned night-heron and Streptopelia turtur - european turtle-dove), 2 endangered species (Egretta garzetta - small egret and Ardea alba – great white egret) included in The Red Book of Vertebrates in Romania, and 13 species that require special conservation measures according to the Annex 1 of the Birds’ Directive

A través del espejo : El cuerpo en la obra de Julio Cortázar

Resumen temporalmente no disponible. La presente obra no cuenta con resumen provisto por el autor.Fil. Gache, Belén. Universidad de Buenos Aires. Facultad de Filosofía y Letras

Contribution à l'étude de la symbolique des animaux dans l'oeuvre "Chantecler" d'Edmond Rostand

Chantecler est une pièce en quatre actes, en vers, d'Edmond Rostand, représentée pour la première fois à Paris en février 1910. Elle met en scène, autour de Chantecler (le Coq), des animaux de basse cour, de jardin et des bois. Chantecler croit avoir pour mission de faire lever le soleil, auquel il chante un hymne. Cependant ses ennemis font en sorte qu'il perde cette illusion. Il n'en continuera pas moins à remplir sa fonction: après la foi perdue, il reste le devoir Une étude des différents personnages de la pièce montre que ces animaux symboliques représentent les prétentions et les jalousies des hommes, en particulier des milieux littéraires. Chantecler connut tout au plus un succès d'estime. Et pourtant, la poésie de Rostand y apparaît dans toute sa splendeur

Connaissance des pharmaciens d'officine à propos du droit de prescription des sages-femmes

Introduction : La profession de sage-femme est une profession médicale à compétences définies. L'une d'entre elles nous intéresse particulièrement : le droit de prescription. Nous avons voulu évaluer les connaissances des pharmaciens d'officine, principaux intéressés de la mise en application de cette compétence. Matériel et méthode : L'objectif de ce travail de recherche était d'évaluer les connaissances des pharmaciens d'officine à propos du droit de prescription des sages-femmes ainsi que de faire un état des lieux de l'utilisation des différents moyens de mise à jour des connaissances. Il s'agit d'une étude descriptive, transversale à visée étiologique. Un questionnaire d'évaluation des connaissances des compétences et du droit de prescription des sages-femmes a été distribué aux pharmaciens d'officine de la région Auvergne. Résultats et discussion : Les pharmaciens d'officine peuvent améliorer leurs connaissances des compétences des sages-femmes : par exemple, trop peu savent que la sage-femme a le droit de prescription auprès des nouveau-nés. Cependant, les taux de réponses exactes concernant les différents domaines du droit de prescription des sages-femmes avoisinaient les 2/3 : taux satisfaisants mais perfectibles. En outre, les moyens de mise à jour des connaissances sont inégalement utilisés et semblent inefficaces puisque les dernières modifications législatives ne sont pas connues. Conclusion : Les connaissances des pharmaciens sont inégales en ce qui concerne les différents domaines du droit de prescription des sages-femmes et l'utilisation des différents moyens de mises à jour des connaissances est insuffisante

Considerações sobre a escrita assêmica: o caso de Mirtha Dermisache

The text addresses the writing of Argentine artist Mirtha Dermisache, one of the main devotees of asemic writing. Her texts unfold into a series of different writing systems in which we come across traits that develop from the concepts of repetition, alternation, symmetry and rhythm in a form of writing without a precise semantic content. As they lack words, the reader can only look at them.El texto aborda la escritura de la artista argentina Mirtha Dermisache, una de las principales cultoras de la escritura asémica. Sus textos se despliegan en una serie de sistemas escriturarios diferentes en los que nos enfrentamos con unos trazos que se desarrollan a partir de los conceptos de repetición, alternancia, simetria y ritmo en una forma de escritura sin um contenido semántico preciso. Como carecen de palabras, el lector solo puede mirarlas.  O texto aborda a escrita da artista argentina Mirtha Dermisache, uma das principais devotas da escrita assêmica. Seus textos se desdobram em uma série de diferentes sistemas de escrita nos quais nos deparamos com traços que se desenvolvem a partir dos conceitos de repetição, alternância, simetria e ritmo em uma forma de escrita sem um conteúdo semântico preciso. Como carecem de palavras, o leitor só pode olhar para elas

Formación online posgrado en terapia manual ortopédica: impacto en los fisioterapéutas de Marbella

La fisioterapia es una disciplina sanitaria que se basa en el empleo de medios físicos para el tratamiento de los pacientes, entre los que se encuentra el uso de las manos del profesional para técnicas de manipulación y movilización. Esto se denomina Terapia Manual Ortopédica (TMO). La formación en este tipo de técnicas supone la adquisición de competencias específicas como la sensibilidad y la especificidad de manejo que pueden resultar un obstáculo para la formación online. El objetivo de este trabajo de investigación trata de establecer si los conocimientos iniciales adquiridos en el grado universitario, junto con las competencias que se trabajan en los cursos online posgrado, son suficientes para garantizar la calidad de este tipo de formación. Para ello, se va a utilizar una metodología cualitativa que nos proporcionará los datos relevantes para valorar la calidad de la docencia online en la TMO y poder abrir una nueva vía de estudio de investigación en el futuro.Universidad Europea de ValenciaMáster Universitario en Docencia UniversitariaVirtua