Pairwise hydrodynamic interactions and diffusion in a vesicle suspension

The hydrodynamic interaction of two deformable vesicles in shear flow induces a net displacement, in most cases an increase of their distance in the transverse direction. The statistical average of these interactions leads to shear-induced diffusion in the suspension, both at the level of individual particles which experience a random walk made of successive interactions, and at the level of suspension where a non-linear down-gradient diffusion takes place, an important ingredient in the structuring of suspension flows. We make an experimental and computational study of the interaction of a pair of lipid vesicles in shear flow by varying physical parameters, and investigate the decay of the net lateral displacement with the distance between the streamlines on which the vesicles are initially located. This decay and its dependency upon vesicle properties can be accounted for by a simple model based on the well established law for the lateral drift of a vesicle in the vicinity of a wall. In the semi-dilute regime, a determination of self-diffusion coefficients is presented

Fibroblast growth factor receptor 4 single nucleotide polymorphism Gly388Arg in head and neck carcinomas

BACKGROUND Head and neck squamous cell carcinoma (HNSCC) is considered to be a progressive disease resulting from alterations in multiple genes regulating cell proliferation and differentiation like receptor tyrosine kinases (RTKs) and members of the fibroblast growth factor receptors (FGFR)-family. Single-nucleotide polymorphism (SNP) Arg388 of the FGFR4 is associated with a reduced overall survival in patients with cancers of various types. We speculate that FGFR4 expression and SNP is associated with worse survival in patients with HSNCC. AIM To investigate the potential clinical significance of FGFR4 Arg388 in the context of tumors arising in HNSCC, a comprehensive analysis of FGFR4 receptor expression and genotype in tumor tissues and correlated results with patients' clinical data in a large cohort of patients with HNSCC was conducted. METHODS Surgical specimens from 284 patients with HNSCC were retrieved from the Institute of Pathology at the Ludwig-Maximilian-University in Germany. Specimens were analyzed using immunohistochemistry and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The expression of FGFR4 was analyzed in 284 surgical specimens of HNSCC using immunohistochemstry. FGFR4 polymorphism was detected by PCR-RFLP. Patients' clinical data with a minimum follow-up of 5 syears were statistically evaluated with a special emphasis on survival analysis employing Kaplan-Meier estimator and Cox regression analysis. RESULTS Concerning the invasive tumor areas the intensity of the FGFR4 expression was evaluated in a four-grade system: no expression, low expression, intermediate and high expression. FGFR4 expression was scored as "high" (+++) in 74 (26%), "intermediate" (++) in 103 (36.3%), and "low" (+) in 107 (36.7%) cases. Analyzing the FGFR4 mutation it was found in 96 tumors (33.8%), 84 of them (29.6%) having a heterozygous and 12 (4.2%) homozygous mutated Arg388 allele. The overall frequency concerning the mutant alleles demonstrated 65% vs 34% mutated alleles in general. FGFR4 Arg388 was significantly associated with advanced tumor stage (P < 0.004), local metastasis (P < 0.0001) and reduced disease-free survival (P < 0.01). Furthermore, increased expression of FGFR4 correlated significantly with worse overall survival (P < 0.003). CONCLUSION In conclusion, the FGFR4 Arg388 genotype and protein expression of FGFR4 impacts tumor progression in patients with HNSCC and may present a useful target within a multimodal therapeutic intervention

Backaction in metasurface etalons

We consider the response of etalons created by a combination of a conventional mirror and a metasurface, composed of a periodic lattice of metal scatterers with a resonant response. This geometry has been used previously for perfect absorption, in so-called Salisbury screens, and for hybridization of localized plasmons with Fabry-Perot resonances. The particular aspect we address is if one can assume an environment-independent reflectivity for the metasurface when calculating the reflectivity of the composite system, as in a standard Fabry-Perot analysis, or whether the fact that the metasurface interacts with its own mirror image renormalizes its response. Using lattice sum theory, we take into account all possible retarded dipole-dipole interactions of scatterers in the metasurface amongst each other, and through the mirror. We show that while a layer-by-layer Fabry-Perot formalism captures the main qualitative features of metasurface etalons, in fact the mirror modifies both the polarizability and reflectivity of the metasurface in a fashion that is akin to Drexhage's modification of the radiative properties of a single dipole.Comment: 10 pages, 5 figure

Phylogeny of sea cucumber (echinodermata: holothuroidea) as inferred from 16s mitochondrial rRNa gene sequences

This study aimed to determine phylogenetic relationship between and among selected species of sea cucumbers (Echinodermata: Holothuroidea) using 16S mitochondrial ribosomal RNA (rRNA) gene. Phylogenetic analyses of 37 partial sequences of 16S mitochondrial rRNA gene using three main methods namely neighbour joining (NJ), maximum parsimony (MP) and maximum likelihood (ML) showed the presence of five main genera of sea cucumbers: Molpadia from order Molpadiida and four genera of order Aspidochirotida namely Holothuria, Stichopus, Bohadschia and Actinopyga. All of the 17 species obtained from Malaysia distributed among the main genera except within Actinopyga. Interestingly, Holothuria excellens was out of Holothuria group causing Holothuria to be paraphyletic. High bootstrap value and consistent clustering made Molpadia, Stichopus, Bohadschia and Actinopyga monophyletic. The relationship of Actinopyga with the other genera was unclarified and Stichopus was sister to Molpadia. The latter finding caused the resolution at order level unclear. The pairwise genetic distance calculated using Kimura 2-parameter model further supported and verified findings from the phylogenetic trees. Further studies with more samples and different mitochondrial DNA genes need to be done to get a better view and verification on the molecular phylogeny of sea cucumber

Analyses multifractales et spatio-temporelles des précipitations du modèle Méso-NH et des données radar

International audienceDans le cadre des multifractals universels, il est possible de caractériser la variabilité spatio-temporelle de la pluie sur une grande gamme d'échelle à l'aide de trois paramètres invariants d'échelles. Dans cette étude, nous avons estimé ces paramètres multifractals sur des simulations numériques effectuées avec le modèle méso-échelle Méso-NH (développé par Météo - France et le Laboratoire d'Aérologie), et des images radar composites, couvrant le même événement pluvieux, à savoir un orage particulièrement violent, dit de type Cévenol, ayant eu lieu sur la partie sud de la France du 5 au 9 septembre 2005. La comparaison des résultats montre que les deux types de données présentent des domaines d'invariance d'échelle relativement similaires, et dont les propriétés sont en accord avec les modèles de précipitation spatio-temporels unifiés et scalant les plus simples. Néanmoins l'évaluation de leurs exposants conduit à des valeurs parfois fortement différentes

Phylogeny of sea cucumber (echinodermata: holothuroidea) as inferred from 16s mitochondrial rRNa gene sequences

This study aimed to determine phylogenetic relationship between and among selected species of sea cucumbers (Echinodermata: Holothuroidea) using 16S mitochondrial ribosomal RNA (rRNA) gene. Phylogenetic analyses of 37 partial sequences of 16S mitochondrial rRNA gene using three main methods namely neighbour joining (NJ), maximum parsimony (MP) and maximum likelihood (ML) showed the presence of five main genera of sea cucumbers: Molpadia from order Molpadiida and four genera of order Aspidochirotida namely Holothuria, Stichopus, Bohadschia and Actinopyga. All of the 17 species obtained from Malaysia distributed among the main genera except within Actinopyga. Interestingly, Holothuria excellens was out of Holothuria group causing Holothuria to be paraphyletic. High bootstrap value and consistent clustering made Molpadia, Stichopus, Bohadschia and Actinopyga monophyletic. The relationship of Actinopyga with the other genera was unclarified and Stichopus was sister to Molpadia. The latter finding caused the resolution at order level unclear. The pairwise genetic distance calculated using Kimura 2-parameter model further supported and verified findings from the phylogenetic trees. Further studies with more samples and different mitochondrial DNA genes need to be done to get a better view and verification on the molecular phylogeny of sea cucumber

Particle Size Distribution of Copper Mine Tailings from Lohan Ranau Sabah and its Relationship with Heavy Metal Content

Particle size analyses were carried out on copper mine tailings from Lohan, Ranau Sabah. About 99.64% of the tailing particles were < 1000 /lm in size while 52.23%,15.72% and 4.87% were < 125 j.l.m, < 62.5 pm and < 38 j.l.m/respectively. The amount of heavy metal extracted by 0.5 HCI was in the order Mn > Cu > Ni > Zn > Co > Cd and is relatively higher in the finer tailing particles

Warehouse Selection for Storage of Finished Goods

In selecting a warehouse for storage of finished goods, from qualitative and quantitative data, aggregate value was obtained as a requirement to make choices and determine costs

Green roof and storm water management policies: monitoring experiments on the ENPC Blue Green Wave

International audienceCurrently widespread in new urban projects, green roofs have shown a positive impact on urban runoff at the building/parcel scale. Nevertheless, there is no specific policy promoting their implementation neither in Europe nor in France. Moreover they are not taken into account (and usually considered as an impervious area) in the sizing of a retention basin for instance. An interesting example is located in the heart of the Paris-East Cluster for Science and Technology (Champs-sur-Marne, France). Since 2013 a large (1 ha) wavy-form vegetated roof (called bleu green wave) is implemented. Green roof area and impervious areas are connected to a large retention basin, which has been oversized. The blue green wave represents a pioneering site where an initially amenity (decorative) design project has been transformed into a research oriented one. Several measurement campaigns have been conducted to investigate and better understand the hydrological behaviour of such a structure. Rainfall, humidity, wind velocity, water content and temperature have been particularly studied. The data collected are used for several purposes: (i) characterize the spatio-temporal variability of the green roof response, (ii) calibrate and validate a specific model simulating its hydrological behavior. Based on monitoring and modeling results, green roof performances will be quantified. It will be possible to estimate how they can reduce stormwater runoff and how these performances can vary in space and in time depending on green roof configuration, rainfall event characteristics and antecedent conditions. These quantified impacts will be related to regulation rules established by stormwater managers in order to connect the parcel to the sewer network. In the particular case of the building of a retention basin, the integration of green roof in the sizing of the basin will be studied