Biomass production and land use management in the Italian context: regulations, conflicts, and impacts

Renewable energy sources, such as biomass can make a positive impact on climate change phenomenon by decreasing our dependence on fossil fuels. The use of biomass energy is directly linked to the use of the land, from which biomass feedstock is obtained, such as farm land and forests, and its ecosystem services. The biomass production and the use of land and ecosystem services are usually associated with a wide range of environmental and social impacts, depending on what choices are made regarding what types of biomass are used, as well as where and how they are produced. Choosing management practices that minimize negative impacts and complement planning policies and energy production objectives is often associated with land-use conflicts among both different institutional levels, local, national and European, and different social actors. Yet, European Directive 2009/28/CE establishes that the energy production from renewable energy by 2020, as well as from biofuel, defined for each member state (Annex 1), must be achieved through a “sustainable†production. Such definition is assigned to national and local contexts, arising issues in policy making, conflicts analysis and methodologies. The present paper discusses on the recent acknowledgment of the above mentioned EU directive in several Italian Regions, such as Puglia and Marche, which have defined regulations/guidelines regarding their potential contribution to the national objectives of production and consumption of energy from renewable sources (EFR). Moreover, the present paper confronts such regulations with results found in literature. Several analyses have been done on the energy production from biomass based on technical and economic aspects of the problem. However, few studies have applied integrated approaches able to take into consideration crucial aspects such as biodiversity conservation and landscape fragmentation, as required by EU Directive 2009/28/CE, side by side with the economic and social dimensions. This paper aims at filling this gap proposing the application of an integrated framework of analysis, based on multi-criteria approaches able to take into consideration socio-economic, environmental and landscape criteria, as well as institutional and social conflicts linked to the biomass production.

Italian hybrid fire prevention code

Fire safety of residential buildings and activities subjected to fire inspection is a difficult task, especially when the safety targets have to be adopted in built buildings or in activities that are going to be modified into more complex ones. Generally, these circumstances show more constraints and it could be difficult to achieve an acceptable level of fire residual risk by prescriptive based fireregulations. Therefore, the Italian National Fire Rescue and Service in charge for fire safety, in August 2015 issued a new Fire Prevention Code whose design methodology is more oriented to fire performance based design rather than prescriptive fire codes. The flexibility of this new fire design methodology offers a very complex tool to experts in order to design fire safety measures and strategies of buildings and activities subjected to fire inspection. The present paper aims tohighlig hts the contents and the fire safety strategy design methodology of the new Italian Fire Prevention Code

The diversity of Class II transposable elements in mammalian genomes has arisen from ancestral phylogenetic splits during ancient waves of proliferation through the genome

DNA transposons make up three percent of the human genome, roughly the same percentage as genes. However, due to their inactivity, they are often ignored in favour of the more abundant, active, retroelements. Despite this relative ignominy, there are a number of interesting questions to be asked of these transposon families. One particular question relates to the timing of proliferation and inactivation of elements in a family. Does an ongoing process of turnover occur, or is the process more akin to a life cycle for the family, with elements proliferating rapidly before deactivation at a later date? We answer this question by tracing back to the most recent common ancestor of each modern transposon family, using two different methods. The first method identifies the most recent common ancestor of the species in which a family of transposon fossils can still be found, which we assume will have existed soon after the true origin date of the transposon family. The second method uses molecular dating techniques to predict the age of the most recent common ancestor element from which all elements found in a modern genome are descended. Independent data from five pairs of species are used in the molecular dating analysis: Human- Chimpanzee, Human-Orangutan, Dog-Panda, Dog-Cat and Cow-Pig. Orthologous pairs of elements from host species pairs are included, and the divergence dates of these species are used to constrain the analysis. We discover that, in general, the times to element common ancestry, for a given family, are the same for the different species pairs, suggesting that there has been no order-specific process of turnover. Furthermore, for most families, the ages of the common ancestor of the host species and of that of the elements are similar, suggesting a life cycle model for the proliferation of transposons. Where these two ages differ, in families found only in Primates and Rodentia, for example, we find that the host species date is later than that of the common ancestor of the elements, implying that there may be large deletions of elements from host species, examples of which were found in their ancestors

Deep Sequencing of Mixed Total DNA without Barcodes Allows Efficient Assembly of Highly Plastic Ascidian Mitochondrial Genomes

Ascidians or sea squirts form a diverse group within chordates, which includes a few thousand members of marine sessile filter-feeding animals. Their mitochondrial genomes are characterized by particularly high evolutionary rates and rampant gene rearrangements. This extreme variability complicates standard polymerase chain reaction (PCR) based techniques for molecular characterization studies, and consequently only a few complete Ascidian mitochondrial genome sequences are available. Using the standard PCR and Sanger sequencing approach, we produced the mitochondrial genome of Ascidiella aspersa only after a great effort. In contrast, we produced five additional mitogenomes (Botrylloides aff. leachii, Halocynthia spinosa, Polycarpa mytiligera, Pyura gangelion, and Rhodosoma turcicum) with a novel strategy, consisting in sequencing the pooled total DNA samples of these five species using one Illumina HiSeq 2000 flow cell lane. Each mitogenome was efficiently assembled in a single contig using de novo transcriptome assembly, as de novo genome assembly generally performed poorly for this task. Each of the new six mitogenomes presents a different and novel gene order, showing that no syntenic block has been conserved at the ordinal level (in Stolidobranchia and in Phlebobranchia). Phylogenetic analyses support the paraphyly of both Ascidiacea and Phlebobranchia, with Thaliacea nested inside Phlebobranchia, although the deepest nodes of the Phlebobranchia–Thaliacea clade are not well resolved. The strategy described here thus provides a cost-effective approach to obtain complete mitogenomes characterized by a highly plastic gene order and a fast nucleotide/amino acid substitution rate

Evolutionary Analysis of Mitogenomes from Parasitic and Free-Living Flatworms

Copyright: © 2015 Solà et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. The attached file is the published version of the article

The Mechanisms of Codon Reassignments in Mitochondrial Genetic Codes

Many cases of non-standard genetic codes are known in mitochondrial genomes. We carry out analysis of phylogeny and codon usage of organisms for which the complete mitochondrial genome is available, and we determine the most likely mechanism for codon reassignment in each case. Reassignment events can be classified according to the gain-loss framework. The gain represents the appearance of a new tRNA for the reassigned codon or the change of an existing tRNA such that it gains the ability to pair with the codon. The loss represents the deletion of a tRNA or the change in a tRNA so that it no longer translates the codon. One possible mechanism is Codon Disappearance, where the codon disappears from the genome prior to the gain and loss events. In the alternative mechanisms the codon does not disappear. In the Unassigned Codon mechanism, the loss occurs first, whereas in the Ambiguous Intermediate mechanism, the gain occurs first. Codon usage analysis gives clear evidence of cases where the codon disappeared at the point of the reassignment and also cases where it did not disappear. Codon disappearance is the probable explanation for stop to sense reassignments and a small number of reassignments of sense codons. However, the majority of sense to sense reassignments cannot be explained by codon disappearance. In the latter cases, by analysis of the presence or absence of tRNAs in the genome and of the changes in tRNA sequences, it is sometimes possible to distinguish between the Unassigned Codon and Ambiguous Intermediate mechanisms. We emphasize that not all reassignments follow the same scenario and that it is necessary to consider the details of each case carefully.Comment: 53 pages (45 pages, including 4 figures + 8 pages of supplementary information). To appear in J.Mol.Evo

Group Portrait:the Ispettrici Nazionali of the Italian Fascist Party 1937-1943

The years of fascist rule in Italy saw an unprecedented mass political mobilization of women, a mobilization that has, to date, been little studied by historians. This article focuses on the role of the ispettrici nazionali – the highest rank that women ever reached in the fascist party hierarchy. It attempts to piece together a ‘group portrait’ of these hitherto unstudied female hierarchs, who were appointed from 1937 onwards to form a group leadership for the fasci femminili – the women's section of the party and the only way that women could join it. The article investigates who these women were, how they managed to rise to this prominent position, their ideas and motivations, and their role in organizing and mobilizing millions of female party members for political campaigns and for the war effort

Challenges of implementing maritime spatial planning in the Eastern Mediterranean

The Maritime Spatial Planning Directive (2014/89/EU) was launched to create a common framework for maritime spatial planning (MSP) in Europe. The case of the Adriatic and Ionian Region (AIR) is emblematic of the peculiar challenges to be faced while implementing MSP in the region

Migración haitiana en Santiago de Chile: Expulsiones, imaginarios e inserción social en un Estado-nación neoliberal

The objective of this article is to characterize and analyze Haitian migration in Chile, in the global context in which it occurs, addressing it from a micro and meso level, understanding from its subjectivities the vulnerable relationships established with the different actors with which they interact in life daily and the role they play in the gradual transformation of social and work spaces. The type of research is qualitative, through the ethnographic method. It is observed that Haitian migration is a challenge for the Chilean State in terms of demand for services and coexistence, located in the field of economic, social, political and cultural rights. Particularly, Haitian migration anthropologically questions the idea of Chilean spatial, temporal and identity boundaries, offering various plans on the possibility of improving their lives and overcoming the barriers they face in a neo-liberal society. It is concluded that Haitians tend to be racialized and excluded, being subalternized, however, to the extent that they manage to work in a stable manner, they are inserted and even generate a willingness to take root considering that the possibility of returning to Haiti is quite uncertain, given the serious political-economic problems from your country of origin.El objetivo del presente artículo es caracterizar y analizar la migración haitiana en Chile, en el contexto global en que ocurre, abordándolo desde un nivel micro y meso, comprendiendo desde sus subjetividades las vulnerables relaciones que establecen con los distintos actores con que interactúan en la vida cotidiana y el papel que juegan en la paulatina transformación de espacios sociales y laborales. El tipo de investigación es cualitativo, a través del método etnográfico. Se observa que la migración haitiana en un desafío para el Estado chileno en términos de demanda de servicios y convivencia, ubicado en el ámbito de los derechos económicos, sociales, políticos y culturales. Particularmente la migración haitiana cuestiona antropológicamente la idea de los límites espaciales, temporales e identitarios chilenos, ofreciendo diversos planos sobre la posibilidad de mejorar su vida y superar las barreras que enfrentan en una sociedad neoliberal. Se concluye que los/as haitianos/as tienden a ser racializados y excluidos, quedando subalternizados, sin embargo, en la medida que logran trabajar de manera estable, se insertan e incluso generan una disposición al arraigo considerando que la posibilidad del regreso hacia Haití es bastante incierta, dados los graves problemas político-económicos de su país de origen

Comparative genomics provides an operational classification system and reveals early emergence and biased spatio-temporal distribution of SARS-CoV-2

Effective systems for the analysis of molecular data are of fundamental importance for real-time monitoring of the spread of infectious diseases and the study of pathogen evolution. While the Nextstrain and GISAID portals offer widely used systems for the classification of SARS-CoV-2 genomes, both present relevant limitations. Here we propose a highly reproducible method for the systematic classification of SARS-CoV-2 viral types. To demonstrate the validity of our approach, we conduct an extensive comparative genomic analysis of more than 20,000 SARS-CoV-2 genomes. Our classification system delineates 12 clusters and 4 super-clusters in SARS-CoV-2, with a highly biased spatio-temporal distribution worldwide, and provides important observations concerning the evolutionary processes associated with the emergence of novel viral types. Based on the estimates of SARS-CoV-2 evolutionary rate and genetic distances of genomes of the early pandemic phase, we infer that SARS-CoV-2 could have been circulating in humans since August-November 2019. The observed pattern of genomic variability is remarkably similar between all clusters and super-clusters, being UTRs and the s2m element, a highly conserved secondary structure element, the most variable genomic regions. While several polymorphic sites that are specific to one or more clusters were predicted to be under positive or negative selection, overall, our analyses also suggest that the emergence of novel genome types is unlikely to be driven by widespread convergent evolution and independent fixation of advantageous substitutions. While, in the absence of rigorous experimental validation, several questions concerning the evolutionary processes and the phenotypic characteristics (increased/decreased virulence) remain open, we believe that the approach outlined in this study can be of relevance for the tracking and functional characterization of different types of SARS-CoV-2 genomes