Multi-Gigabit Wireless data transfer at 60 GHz

In this paper we describe the status of the first prototype of the 60 GHz wireless Multi-gigabit data transfer topology currently under development at University of Heidelberg using IBM 130 nm SiGe HBT BiCMOS technology. The 60 GHz band is very suitable for high data rate and short distance applications as for example needed in the HEP experments. The wireless transceiver consist of a transmitter and a receiver. The transmitter includes an On-Off Keying (OOK) modulator, an Local Oscillator (LO), a Power Amplifier (PA) and a BandPass Filter (BPF). The receiver part is composed of a BandPass- Filter (BPF), a Low Noise Amplifier (LNA), a double balanced down-convert Gilbert mixer, a Local Oscillator (LO), then a BPF to remove the mixer introduced noise, an Intermediate Amplifier (IF), an On-Off Keying demodulator and a limiting amplifier. The first prototype would be able to handle a data-rate of about 3.5 Gbps over a link distance of 1 m. The first simulations of the LNA show that a Noise Figure (NF) of 5 dB, a power gain of 21 dB at 60 GHz with a 3 dB bandwidth of more than 20 GHz with a power consumption 11 mW are achieved. Simulations of the PA show an output referred compression point P1dB of 19.7 dB at 60 GHz.Comment: Proceedings of the WIT201

Modulation of LAT1 (SLC7A5) transporter activity and stability by membrane cholesterol.

LAT1 (SLC7A5) is a transporter for both the uptake of large neutral amino acids and a number of pharmaceutical drugs. It is expressed in numerous cell types including T-cells, cancer cells and brain endothelial cells. However, mechanistic knowledge of how it functions and its interactions with lipids are unknown or limited due to inability of obtaining stable purified protein in sufficient quantities. Our data show that depleting cellular cholesterol reduced the Vmax but not the Km of the LAT1 mediated uptake of a model substrate into cells (L-DOPA). A soluble cholesterol analogue was required for the stable purification of the LAT1 with its chaperon CD98 (4F2hc,SLC3A2) and that this stabilised complex retained the ability to interact with a substrate. We propose cholesterol interacts with the conserved regions in the LAT1 transporter that have been shown to bind to cholesterol/CHS in Drosophila melanogaster dopamine transporter. In conclusion, LAT1 is modulated by cholesterol impacting on its stability and transporter activity. This novel finding has implications for other SLC7 family members and additional eukaryotic transporters that contain the LeuT fold

Australian human and parrot Chlamydia psittaci strains cluster within the highly virulent 6BC clade of this important zoonotic pathogen

Chlamydia psittaci is an avian pathogen and zoonotic agent of atypical pneumonia. The most pathogenic C. psittaci strains cluster into the 6BC clade, predicted to have recently emerged globally. Exposure to infected parrots is a risk factor with limited evidence also of an indirect exposure risk. Genome sequencing was performed on six Australian human and a single avian C. psittaci strain isolated over a 9 year period. Only one of the five human patients had explicit psittacine contact. Genomics analyses revealed that the Australian C. psittaci strains are remarkably similar, clustering tightly within the C. psittaci 6BC clade suggested to have been disseminated by South America parrot importation. Molecular clock analysis using the newly sequenced C. psittaci genomes predicted the emergence of the 6BC clade occurring approximately 2,000 years ago. These findings reveal the potential for an Australian natural reservoir of C. psittaci 6BC strains. These strains can also be isolated from seriously ill patients without explicit psittacine contact. The apparent recent and global spread of C. psittaci 6BC strains raises important questions over how this happened. Further studies may reveal whether the dissemination of this important zoonotic pathogen is linked to Australian parrot importation rather than parrots from elsewhere

Culture-independent genome sequencing of clinical samples reveals an unexpected heterogeneity of infections by chlamydia pecorum

Copyright © 2015, American Society for Microbiology. All Rights Reserved. Chlamydia pecorum is an important global pathogen of livestock, and it is also a significant threat to the long-term survival of Australia's koala populations. This study employed a culture-independent DNA capture approach to sequence C. pecorum genomes directly from clinical swab samples collected from koalas with chlamydial disease as well as from sheep with arthritis and conjunctivitis. Investigations into single-nucleotide polymorphisms within each of the swab samples revealed that a portion of the reads in each sample belonged to separate C. pecorum strains, suggesting that all of the clinical samples analyzed contained mixed populations of genetically distinct C. pecorum isolates. This observation was independent of the anatomical site sampled and the host species. Using the genomes of strains identified in each of these samples, whole-genome phylogenetic analysis revealed that a clade containing a bovine and a koala isolate is distinct from other clades comprised of livestock or koala C. pecorum strains. Providing additional evidence to support exposure of koalas to Australian livestock strains, two minor strains assembled from the koala swab samples clustered with livestock strains rather than koala strains. Culture-independent probebased genome capture and sequencing of clinical samples provides the strongest evidence yet to suggest that naturally occurring chlamydial infections are comprised of multiple genetically distinct strains

Early MicroRNA expression profile as a prognostic biomarker for the development of pelvic inflammatory disease in a mouse model of chlamydial genital infection

It is not currently possible to predict the probability of whether a woman with a chlamydial genital infection will develop pelvic inflammatory disease (PID). To determine if specific biomarkers may be associated with distinct chlamydial pathotypes, we utilized two Chlamydia muridarum variants (C. muridarum Var001 [CmVar001] and CmVar004) that differ in their abilities to elicit upper genital tract pathology in a mouse model. CmVar004 has a lower growth rate in vitro and induces pathology in only 20% of C57BL/6 mouse oviducts versus 83.3% of oviducts in CmVar001-infected mice. To determine if chemokine and cytokine production within 24 h of infection is associated with the outcome of pathology, levels of 15 chemokines and cytokines were measured. CmVar004 infection induced significantly lower levels of CXCL1, CXCL2, tumor necrosis factor alpha (TNF-α), and CCL2 in comparison to CmVar001 infection with similar rRNA (rs16) levels for Chlamydiae. A combination of microRNA (miRNA) sequencing and quantitative real-time PCR (qRT-PCR) analysis of 134 inflammation-related miRNAs was performed 24 h postinfection to determine if the chemokine/cytokine responses would also be reflected in miRNA expression profiles. Interestingly, 12 miRNAs (miR-135a-5p, miR298-5p, miR142-3p, miR223-3p, miR299a-3p, miR147-3p, miR105, miR325-3p, miR132-3p, miR142-5p, miR155-5p, and miR-410-3p) were overexpressed during CmVar004 infection compared to CmVar001 infection, inversely correlating with the respective chemokine/cytokine responses. To our knowledge, this is the first report demonstrating that early biomarkers elicited in the host can differentiate between two pathological variants of chlamydiae and be predictive of upper tract disease. © 2014 Yeruva et al

A CAPTCHA model based on visual psychophysics: Using the brain to distinguish between human users and automated computer bots

Demand for the use of online services such as free emails, social networks, and online polling is increasing at an exponential rate. Due to this, online service providers and retailers feel pressurised to satisfy the multitude of end-user expectations. Meanwhile, automated computer robots (known as “bots”) are targeting online retailers and service providers by acting as human users and providing false information in order to abuse their service provisioning. CAPTCHA is a set of challenge/response protocol, which was introduced to protect online retailers and service providers from misuse and automated computer attacks. Text-based CAPTCHAs are the most popular form, and are used by most online service providers to differentiate between the human users and bots. However, the vast majority of text-based CAPTCHAs have been broken using the Optical Character Recognition (OCR) techniques and thus, reinforces the need for developing a secure and robust CAPTCHA model. Security and usability are the two fundamental issues that pose a trade-off in the design of a CAPTCHA; a hard CAPTCHA model could also be difficult for human users to resolve, which affects its usability, and vice versa. The model developed in this study uses the unsurpassed abilities of the Human Visual System (HVS) to superimpose and integrate complex information presented in individual frames, using the mechanism of trans-saccadic memory. In this context, the model integrates in its design the concept of persistence of vision, which enables humans to see the world in a continuous fashion. Preliminary results from the proposed model based on this technique are encouraging. To ensure the usability of the proposed CAPTCHA model, we set the threshold for the ORO parameter at 40%. This ensured that our CAPTCHA strings would be recognised by human observers at a rate of over 99% (or as close to 100% as is realistic). In turn, when examining the robustness of our VICAP model to computer programme attacks, we can observe that for the traditional case of OCR recognition, based on a single-frame scenario, the Computer Recognition Success Rate (CRSR) was about 0%, while in the case of a multi-frame scenario, the CRSR could increase to up to 50%

Drag Show, 2019

Photographs from the annual Drag Show put on by PRISM (now GSA) in 2019 featuring Drag Queens from Joplin, MO and student performers in the U-Club.https://digitalcommons.pittstate.edu/gsadragshow/1000/thumbnail.jp

Dynamic response and robustness of tall buildings under blast loading

Recently, extensive research has been focused on the progressive collapse analysis of the multi-storey buildings. However, most of the research is based on the Alternative Path Method (APM) with sudden removal of the columns, ignoring the duration of the blast load working on the structures. In this paper, a 3-D numerical model with the direct simulation of blast load is proposed to study the real behaviour of a 20 storey tall building under the blast loading. A typical package bomb charge of 15 kg was detonated on the 12th floor. The corresponding dynamic response of structure was studied in details. The robustness of the building under blast load was assessed. Comparison between the proposed method and the APM was also made. It is found that, due to the uplift and downw ard pressure working on the slab, the column force under the direct blast simulation method is smaller than that of the alternative path method. The method to enhance the robustness of the buildings is also recommended

Study on collapse mechanism of steel frame with CFST-columns under column-removal scenario

The design of structure against progressive collapse has tended towards more quantitative design where utilizing catenary action becomes essential. In this paper, a single internal column removal test was conducted for a 1/3 scale 4-bay steel frame with concrete-filled steel tubular (CFST) columns. The anti-collapse mechanism of the frame under the scenario of column loss is discussed. Both FE model and simplified analytical model are developed to investigate the behavior of steel frame with CFST columns in resisting progressive collapse. The accuracy of the two models is verified through the experimental results. The anti-collapse measures of the proposed model are sensitive to the modeling techniques used to simulate the CFST columns. A method based on the energy equivalence is used to evaluate the dynamic behavior of the frame. The results show that the DAF (dynamic amplification factor) value of 2.0 which is recommended by DoD provision in linear static analysis is reasonable. However the mobilization of “catenary action” which is not considered in DoD provision would increase the DAF value as currently given in DoD

A laboratory methodology for dual RNA-sequencing of bacteria and their host cells in vitro

© 2017 Marsh, Humphrys and Myers. Dual RNA-Sequencing leverages established next-generation sequencing (NGS)-enabled RNA-Seq approaches to measure genome-wide transcriptional changes of both an infecting bacteria and host cells. By simultaneously investigating both organisms from the same biological sample, dual RNA-Seq can provide unique insight into bacterial infection processes and reciprocal host responses at once. However, the difficulties involved in handling both prokaryotic and eukaryotic material require distinct, optimized procedures. We previously developed and applied dual RNA-Seq to measure prokaryotic and eukaryotic expression profiles of human cells infected with bacteria, using in vitro Chlamydia-infected epithelial cells as proof of principle. Here we provide a detailed laboratory protocol for in vitro dual RNA-Seq that is readily adaptable to any host-bacteria system of interest