Agrobiodiversity endangered by sugarcane farming in Mumias and Nzoia Sugarbelts of Western Kenya

Commercial sugarcane farming has been practised in western Kenya for nearly forty years. This monocultural land use is associated with loss of natural vegetation and cropland, thus undermining food security status of a place. Further, sugarcane farming is a major contributor to loss of biodiversity in western Kenya. This study was therefore aimed at determining the long-term effects of sugarcane farming on indigenous food crops and vegetables in Mumias and Nzoia sugarbelts of western Kenya. Up to 188 respondents in three divisions of Mumias and 178 respondents of three divisions in Nzoia were purposively selected. These included small-scale and large-scale farmers. Data were collected using questionnaires, Participatory Rural Appraisal tool, interviews and field observations. Secondary data were obtained from documented materials. Land under indigenous food crops and vegetable has been declining since the introduction of sugarcane. Indigenous food crops and vegetable cultivation by farmers in the sugarbelts has been declining. Furthermore, some farmers have abandoned the growing of these crops altogether. Our results imply that sugarcane farming is a major contributor to agrobiodiversity erosion, but that there are also other important reasons such as change of consumer preference, land fragmentation, climate variability among others. In order to curb further loss of biodiversity, efforts should particularly focus on food crops and livelihood diversification and adoption of farming technologies such as agroforestry.Key words: Biodiversity, farming, indigenous crops, monoculture, Western Kenya

Infections with Avian Pathogenic and Fecal Escherichia coli Strains Display Similar Lung Histopathology and Macrophage Apoptosis

The purpose of this study was to compare histopathological changes in the lungs of chickens infected with avian pathogenic (APEC) and avian fecal (Afecal) Escherichia coli strains, and to analyze how the interaction of the bacteria with avian macrophages relates to the outcome of the infection. Chickens were infected intratracheally with three APEC strains, MT78, IMT5155, and UEL17, and one non-pathogenic Afecal strain, IMT5104. The pathogenicity of the strains was assessed by isolating bacteria from lungs, kidneys, and spleens at 24 h post-infection (p.i.). Lungs were examined for histopathological changes at 12, 18, and 24 h p.i. Serial lung sections were stained with hematoxylin and eosin (HE), terminal deoxynucleotidyl dUTP nick end labeling (TUNEL) for detection of apoptotic cells, and an anti-O2 antibody for detection of MT78 and IMT5155. UEL17 and IMT5104 did not cause systemic infections and the extents of lung colonization were two orders of magnitude lower than for the septicemic strains MT78 and IMT5155, yet all four strains caused the same extent of inflammation in the lungs. The inflammation was localized; there were some congested areas next to unaffected areas. Only the inflamed regions became labeled with anti-O2 antibody. TUNEL labeling revealed the presence of apoptotic cells at 12 h p.i in the inflamed regions only, and before any necrotic foci could be seen. The TUNEL-positive cells were very likely dying heterophils, as evidenced by the purulent inflammation. Some of the dying cells observed in avian lungs in situ may also be macrophages, since all four avian E. coli induced caspase 3/7 activation in monolayers of HD11 avian macrophages. In summary, both pathogenic and non-pathogenic fecal strains of avian E. coli produce focal infections in the avian lung, and these are accompanied by inflammation and cell death in the infected areas

Determination of the mass of the W boson

Previous studies of the physics potential of LEP2 indicated that with the design luminosity of 500 inverse picobarn one may get a direct measurement of the mass of the W-boson with a precision in the range 30 - 50 MeV. This report presents an updated evaluation of the estimated error on the mass of the W-boson based on recent simulation work and improved theoretical input. The most efficient experimental methods which will be used are also described

Developing locally managed marine areas: lessons learnt from Kenya

Coastal communities in Kenya are increasingly adopting Locally Managed Marine Areas (LMMAs) and by 2015, 24 had been established. Coastal communities perceive the objectives of these LMMAs are to primarily conserve fisheries and marine resources and secure alternative sources of income. In this study we examined if there are generic approaches in how these LMMAs were established, that can be used for developing national guidelines as well as have application to other locations in the western Indian Ocean region. The study involved a literature review of all documents available on the LMMAs and key informant interviews.We found LMMAs in Kenya go through five phases to become fully established and operational: i) Conceptualisation, ii) Inception, iii) Implementation, iv) Monitoring and management; and v) Ongoing Adaptive Management. We defined each stage by the activities that are taking place which determine how far a LMMA has reached in its development. The final phase is when a LMMA exists sustainably in a continuous learning process. Out of 19 LMMAs assessed, four had reached the fifth stage of ‘Ongoing Adaptive Management’ though not all elements of this stage were fully operational.The Kenyan model differs from the widely known Pacific model of four phases due to an additional initial ‘Conceptualisation’ phase. Our results illustrate the need for full acceptance of the LMMA concept by stakeholders before progressing to the ‘Inception phase.’ When this step was missed many LMMAs stalled due to unaddressed training needs, incomplete involvement of stakeholders and lack of financial resources, management and operational structures. These five phases provide a useful guide for communities and other stakeholders to follow when developing LMMAs, or for those that are established and need guidance on their operations.Common factors that we found associated with the development of LMMAs were informed and committed community members, past training in community based marine resource management, a supportive legal framework, external funding and opportunities for sharing LMMA information. The occurrence of an exchange visit to an existing LMMA was invariably the trigger for a community to establish their own LMMA. Weaknesses were seen in poor enforcement on the water and inadequate ongoing education and training. Further there was very little understanding of the costs of establishing and running a LMMA, therefore long term financial sustainability was problematic. Thus, although the rapid increase in the number of LMMAs in Kenya is a conservation success, their effectiveness will be thwarted if enforcement and financial management are not addressed.</div

Factors influencing performance of health workers in the management of seriously sick children at a Kenyan tertiary hospital - Participatory action research

Background: Implementation of World Health Organization case management guidelines for serious childhood illnesses remains a challenge in hospitals in low-income countries. Facilitators of and barriers to implementation of locally adapted clinical practice guidelines (CPGs) have not been explored. Methods. This ethnographic study based on the theory of participatory action research (PAR) was conducted in Kenyatta National Hospital, Kenya's largest teaching hospital. The primary intervention consisted of dissemination of locally adapted CPGs. The PRECEDE-PROCEED health education model was used as the conceptual framework to guide and examine further reinforcement activities to improve the uptake of the CPGs. Activities focussed on introduction of routine clinical audits and tailored educational sessions. Data were collected by a participant observer who also facilitated the PAR over an eighteen-month period. Naturalistic inquiry was utilized to obtain information from all hospital staff encountered while theoretical sampling allowed in-depth exploration of emerging issues. Data were analysed using interpretive description. Results: Relevance of the CPGs to routine work and emergence of a champion of change facilitated uptake of best-practices. Mobilization of basic resources was relatively easily undertaken while activities that required real intellectual and professional engagement of the senior staff were a challenge. Accomplishments of the PAR were largely with the passive rather than active involvement of the hospital management. Barriers to implementation of best-practices included i) mismatch between the hospital's vision and reality, ii) poor communication, iii) lack of objective mechanisms for monitoring and evaluating quality of clinical care, iv) limited capacity for planning strategic change, v) limited management skills to introduce and manage change, vi) hierarchical relationships, and vii) inadequate adaptation of the interventions to the local context. Conclusions: Educational interventions, often regarded as 'quick-fixes' to improve care in low-income countries, may be necessary but are unlikely to be sufficient to deliver improved services. We propose that an understanding of organizational issues that influence the behaviour of individual health professionals should guide and inform the implementation of best-practices. © 2014 Irimu et al.; licensee BioMed Central Ltd

Performance of health workers in the management of seriously sick children at a Kenyan tertiary hospital: before and after a training intervention.

BACKGROUND: Implementation of WHO case management guidelines for serious common childhood illnesses remains a challenge in hospitals in low-income countries. The impact of locally adapted clinical practice guidelines (CPGs) on the quality-of-care of patients in tertiary hospitals has rarely been evaluated. METHODS AND FINDINGS: We conducted, in Kenyatta National Hospital, an uncontrolled before and after study with an attempt to explore intervention dose-effect relationships, as CPGs were disseminated and training was progressively implemented. The emergency triage, assessment and treatment plus admission care (ETAT+) training and locally adapted CPGs targeted common, serious childhood illnesses. We compared performance in the pre-intervention (2005) and post-intervention periods (2009) using quality indicators for three diseases: pneumonia, dehydration and severe malnutrition. The indicators spanned four domains in the continuum of care namely assessment, classification, treatment, and follow-up care in the initial 48 hours of admission. In the pre-intervention period patients' care was largely inconsistent with the guidelines, with nine of the 15 key indicators having performance of below 10%. The intervention produced a marked improvement in guideline adherence with an absolute effect size of over 20% observed in seven of the 15 key indicators; three of which had an effect size of over 50%. However, for all the five indicators that required sustained team effort performance continued to be poor, at less than 10%, in the post-intervention period. Data from the five-year period (2005-09) suggest some dose dependency though the adoption rate of the best-practices varied across diseases and over time. CONCLUSION: Active dissemination of locally adapted clinical guidelines for common serious childhood illnesses can achieve a significant impact on documented clinical practices, particularly for tasks that rely on competence of individual clinicians. However, more attention must be given to broader implementation strategies that also target institutional and organisational aspects of service delivery to further enhance quality-of-care