Did Bacillus megaterium pick up plasmid virulence genes?

Plasmids are known to carry genes that allow bacteria to survive in different environments. Virulence genes that cause bacteria to be pathogenic are also found on plasmids. Bacillus megaterium is a non-pathogenic, spore-forming bacteria that is found in soil, but recently a strain of B. megaterium was reported to cause a mild case of diarrhea in an infant. This appears to be the first case of infection caused by B. megaterium (CHI). Bacillus cereus is a related species and a known gastroenteric pathogen. Research has shown that the pathogenicity in some strains of B. cereus is caused by either an operon containing four genes, hblA/B/C/D, or by a single gene, bceT. The pathogenic strain of B. megaterium, (CHI), was tested by PCR and hybridization, to see if it picked up either of these two factors that cause pathogenicity in B. cereus by plasmid exchange. PCR products were obtained in CHI for the genes hblA and bceT using specific primers suggesting that such genes are present in the B. megaterium (CHI) strain. However, a hybridization experiment using bceT product as a probe for CHI failed to show a signal

Optimization under uncertainty and risk: Quadratic and copositive approaches

Robust optimization and stochastic optimization are the two main paradigms for dealing with the uncertainty inherent in almost all real-world optimization problems. The core principle of robust optimization is the introduction of parameterized families of constraints. Sometimes, these complicated semi-infinite constraints can be reduced to finitely many convex constraints, so that the resulting optimization problem can be solved using standard procedures. Hence flexibility of robust optimization is limited by certain convexity requirements on various objects. However, a recent strain of literature has sought to expand applicability of robust optimization by lifting variables to a properly chosen matrix space. Doing so allows to handle situations where convexity requirements are not met immediately, but rather intermediately. In the domain of (possibly nonconvex) quadratic optimization, the principles of copositive optimization act as a bridge leading to recovery of the desired convex structures. Copositive optimization has established itself as a powerful paradigm for tackling a wide range of quadratically constrained quadratic optimization problems, reformulating them into linear convex-conic optimization problems involving only linear constraints and objective, plus constraints forcing membership to some matrix cones, which can be thought of as generalizations of the positive-semidefinite matrix cone. These reformulations enable application of powerful optimization techniques, most notably convex duality, to problems which, in their original form, are highly nonconvex. In this text we want to offer readers an introduction and tutorial on these principles of copositive optimization, and to provide a review and outlook of the literature that applies these to optimization problems involving uncertainty

Roll Motion of a Water Filled Floating Cylinder—Additional Experimental Verification

Understanding the behaviour of water filled bodies is important from an applied engineering perspective when understanding the sea-keeping performance of certain floating platforms and vessels. Even by assuming that the deformation is negligible small in relation to the motion of the structure, these fluid-structure-fluid interactions are challenging to model, both physically and numerically, and there is a notable lack of reference data sets and studies to support the validation of this work. Most of the existing information is highly specific to certain hulls forms, or is limited to small motions. A previous study addressed this by modelling a floating cylinder (giving a more generic case) with roll and pitch motions in excess of 20°. The presented experiment expands on that work to further investigate the previously observed switch between pitch and roll in the cylinder under wave action as induced by the sloshing of the internal water volume. An additional experimental investigation, focused on a single draft, was conducted to test open research questions from the previous study. Here we show that the roll response of the water filled cylinder is repeatable, independent of the tank position and wave amplitude, provided the observation time is long enough to capture the fully developed motion response of the floating object. The mooring system used comprised four soft lines connected on two points on the cylinder. This arrangement resulted in slightly different restoring forces in different wave directions. A relative change of the wave direction by 90° led to a larger wave frequency band in which the roll motion occurred. These cases were, again, also conducted with the solid ballast. Both sets of data provide an interesting validation case for future work on water ballast inside a floating object

Additional Experimental Data of a Floating Cylinder in a Wave Tank - Verification Experiments

The presented experimental results provide additional data to a previously published investigation of a floating cylinder under regular wave conditions (https://doi.org/10.7488/ds/2627). Three test series are added to investigate the roll response of the water filled structure. This includes a change of the initial position of the cylinder in the wave tank as well as a rotation of the wave direction. The latter was also conducted for the solid ballast option to provide a direct comparison with the water filled cylinder. This research was carried out at the FloWave Ocean Energy Research Facility of the Institute for Energy Systems, University of Edinburgh

Accuracy Analysis of the Measurement of Centre of Gravity and Moment of Inertia with a Swing

Floating devices under wave and current loads are typically designed based on numerical methods followed by a validation with experimental investigations. This allows an independent check due to the comparison of two different modelling approaches based on different assumptions. At an early stage of the project, numerical simulations are based on theoretical (ideal) values of the centre of gravity (CG) and moment of inertia (MI). The building process of a scaled model results very often in a requested simplification of certain parts, which can influence the CG and also the MI of the scaled model. Knowing those discrepancies allows us to improve the comparability of both approaches but the measurement of those values is connected with either a higher uncertainty or a high level of effort. A significant improvement of such measurements can be reached by the deployment of a specific experimental set-up. This paper presents the classification of the newly designed swing with a high accuracy inertial inclinometer, which was verified by the marker-based motion capturing system. The achieved experiences are useful for the future use of the set-up as well as similar investigations. The comparison with the theoretical values for the swing as well as an example model showed very good agreements and a high accuracy of few millimetres for the CG and an error smaller 1% for MI

Isoenzyme der Creatinkinase in der Perinatalperiode

Peer Reviewe

The dispersion of spherical droplets in source–sink flows and their relevance to the COVID-19 pandemic

In this paper, we investigate the dynamics of spherical droplets in the presence of a source-sink pair flow field. The dynamics of the droplets is governed by the Maxey-Riley equation with Basset-Boussinesq history term neglected. We find that, in the absence of gravity, there are two distinct behaviours for the droplets: small droplets cannot go further than a specific distance, which we determine analytically, from the source before getting pulled into the sink. Larger droplets can travel further from the source before getting pulled into the sink by virtue of their larger inertia, and their maximum travelled distance is determined analytically. We investigate the effects of gravity, and we find that there are three distinct droplet behaviours categorised by their relative sizes: small, intermediate-sized, and large. Counterintuitively, we find that the droplets with minimum horizontal range are neither small nor large, but of intermediate size. Furthermore, we show that in conditions of regular human respiration, these intermediate-sized droplets range in size from a few $\mu$m to a few hundred $\mu$m. The result that such droplets have a very short range could have important implications for the interpretation of existing data on droplet dispersion.Comment: 14 pages, 7 figure